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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳克強(Keqiang Wu) | |
| dc.contributor.author | Wei-Ting Shih | en |
| dc.contributor.author | 施威廷 | zh_TW |
| dc.date.accessioned | 2021-06-15T16:44:28Z | - |
| dc.date.available | 2015-08-16 | |
| dc.date.copyright | 2015-08-16 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-10 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/53101 | - |
| dc.description.abstract | WRKY轉錄因子在植物界中是一個很大的蛋白家族。在先前的研究當中，阿拉伯芥WRKY轉錄因子被報導在生物性防禦反應中扮演十分重要的角色，但在非生物逆境與植物生長發育的調控中的作用所知仍然較少。在本研究中我們發現屬於IIIa類的WRKY轉錄因子WRKY63蛋白會和RPD3類的組蛋白去乙酰化酶HDA6有交互作用，我們也觀察到WRKY63的突變株abo3對鹽逆境及離層酸有敏感的表型，並且發現WRKY63和HDA6的雙突變株abo3/axe1-5對鹽逆境會有較單突變更敏感的表型；另外，我們也觀察到abo3突變株有比野生型早開花的表型。透過檢測這些突變株與野生型植株中的基因表達，我們發現鹽逆境反應與開花調控的下游基因表達，相對於野生型在突變株中皆有受到影響。從這些結果我們可以知道WRKY63可能會藉由調控下游基因的表達來影響鹽逆境反應及開花時間的控制。 | zh_TW |
| dc.description.abstract | WRKY transcription factors constitute a large protein family in plants. Previous studies indicated that WRKY transcription factors play important roles in the regulation of gene expression associated with plant defense responses, but the role of WRKY proteins in abiotic stress responses and development is still unclear. In this study, we show that WRKY63, a member of WRKY IIIa subfamily, could interact with the RPD3-like histone deacetylase HDA6. The WRKY63 T-DNA insertion knock-out mutant, abo3, is hypersensitive to ABA and salt stress. Furthermore, abo3/axe1-5 double mutant plants displayed increased salt sensitivity compared with abo3 and axe1-5 plants. In addition, the abo3 mutant showed the early flowering phenotype compared with wild type plants. The expression levels of salt stress-responsive genes as well as genes involved in flowering time control were changed in the abo3 mutants. These data suggested that WRKY63 plays an important role in the regulation of gene expression involved in salt stress responses and flowering time control. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T16:44:28Z (GMT). No. of bitstreams: 1 ntu-104-R02b42019-1.pdf: 4038409 bytes, checksum: 9f15bbbcb5bbca55ed4916d2729aa9d7 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 摘要 …………………………………………………………………………………..I
Abstract ……………………………………………………………………………...II Index………………………………………………………………………………...III List of Tables ……………………………………………………………………….VI List of Figures ………………………………………………………………….….VII List of Supplementary Figures ……………………………………………………..X List of Abbreviations………………………………………………………………..XI Introduction…………………………………………………………………………..1 WRKY transcription factors……………………………………………………….1 WRKY transcription factors involved in biotic stress responses………………….2 WRKY transcription factors involved in abiotic stress responses………………...4 Histone deacetylases (HDAs) in Arabidopsis………….………….………………5 Material and Methods………….…………………………………………...………..8 Plant materials……………………………………………………………………..8 Seed germination in Petri dishes…………………………………………………..8 Measurement of germination rates………………………………………………...9 High salinity treatment………………………………………………………….....9 Quick DNA extraction…………………………………………………………...10 RNA isolation…………………………………………………………………….10 DNase treatment………………………………………………………………….12 Quantitative RT-PCR analysis……………………………………………………12 Chromatin immunoprecipitation assays………………………………………….13 Bimolecular Fluorescence Complementation (BiFC) assays…………………….22 Yeast two-hybrid assays………………………………………………………….27 In vitro pull-down assays………………………………………………………...27 Co-immunoprecipitation assays………………………………………………….28 Results………………………………………………………………………….……31 Phylogenic tree of WRKY transcription factor superfamily……………………..31 WRKY63 interacted with the RPD3-type HDAs in Arabidopsis……………......31 Identification of interaction domains of HDA6 and WRKY63………………….32 Expression of WRKY63 was induced by salt stress………………………………33 Identification of homozygous T-DNA insertion mutants of WRKY63…………...33 wrky63 mutants are hypersensitive to ABA and salt stress………………………34 Expression of stress-responsive genes under the salt treatment………………….35 Analysis of transgenic plants overexpressing WRKY63………………………….36 The abo3/axe1-5 double mutant was hypersensitive to salt stress……………….37 ERD genes were hyperacetylated in abo3/axe1-5 plants………………………...37 WRKY63 disassociated from ERD15 during the salt stress response…………...38 WRKY63 mutants displayed an early flowering phenotype……………………..39 FLC was down-regulated in abo3 mutants………………………………………39 WRKY63 targeted to FCA, FPA, and VIN3……………………………………...40 Vernalization-insensitive phenotype of abo3…………………………………….41 The relationship between WRKY63 and HDA6 in flowering time control……...41 HDA6 physically interacted with FCA and FPA…………………………………42 Discussion..…………………………………………………………………………..43 WRKY63 interacts with HDA6 in Arabidopsis………………………………….43 WRKY63 is involved in salt stress responses in Arabidopsis…………………...44 WRKY63 and HDA6 regulate gene expression through histone deacetylation…45 WRKY63 is involved in flowering time control in Arabidopsis………………...46 Repression of FCA and FPA needed the HDAs activity………………………...46 Figures………………………………………………………………………………48 Supplementary Figures…………………………………………………………….97 Tables……………………………………………………………………………….104 References…………………………………………………………………………..110 Appendix…………………………………………………………………………118 | |
| dc.language.iso | en | |
| dc.subject | WRKY63 | zh_TW |
| dc.subject | 開花時間 | zh_TW |
| dc.subject | 鹽逆境 | zh_TW |
| dc.subject | 阿拉伯芥 | zh_TW |
| dc.subject | 組蛋白去乙醯化? | zh_TW |
| dc.subject | salt stress | en |
| dc.subject | histone deacetylases | en |
| dc.subject | WRKY63 | en |
| dc.subject | flowering time | en |
| dc.subject | Arabidopsis | en |
| dc.title | 阿拉伯芥WRKY63在鹽逆境及開花時間調控的功能性分析 | zh_TW |
| dc.title | Functional analysis of Arabidopsis WRKY63 in salt stress response and flowering time control | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 施明哲(Ming-Che Shih),葉開溫(Kai-Wen Yeh),靳宗洛(Tsung-Luo Jinn),鄭秋萍(Chiu-Ping Cheng) | |
| dc.subject.keyword | 阿拉伯芥,組蛋白去乙醯化?,WRKY63,鹽逆境,開花時間, | zh_TW |
| dc.subject.keyword | Arabidopsis,histone deacetylases,WRKY63,salt stress,flowering time, | en |
| dc.relation.page | 118 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-08-10 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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| ntu-104-1.pdf 未授權公開取用 | 3.94 MB | Adobe PDF |
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