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請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/53094
完整後設資料紀錄
DC 欄位值語言
dc.contributor.advisor廖文亮
dc.contributor.authorYu-Lung Luen
dc.contributor.author陸昱龍zh_TW
dc.date.accessioned2021-06-15T16:44:02Z-
dc.date.available2015-08-20
dc.date.copyright2015-08-20
dc.date.issued2015
dc.date.submitted2015-08-10
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dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/53094-
dc.description.abstract本研究的目的在於探討在飼料中添加不同比例的靈芝固態培養基對南美白蝦在成長表現與非特異性免疫的影響。實驗分為添加0g/kg , 7.5g/kg , 12.5g/kg , 25g/kg , 50g/kg的靈芝固態培養基來分別評估其對白蝦在成長及免疫表現上的影響。成長試驗是透過檢測其飼養6周後的存活率和增重率來做為其成長表現的依據,其結果顯示經過6周的投餵實驗後,靈芝固態培養基不會對白蝦成長有任何影響,惟添加量50g/kg時存活率有下降的現象值得被注意。免疫試驗是透過在餵食實驗飼料後的第0、4、7、10、14天分別檢測其原酚氧化酶活性、吞噬活性、超氧陰離子產生量及溶菌酶活性。實驗結果顯示,超氧陰離子產生量和溶菌酶活性在靈芝固態培養基添加量12.5g/kg時有較好的表現,原酚氧化酶活性和吞噬活性則在靈芝固態培養基添加量12.5g/kg 、50g/kg時有最好的表現能力。
綜合實驗結果發現,添加靈芝固態培養基於白蝦飼料中能有效提高白蝦的非特異性免疫,其中又以添加12.5~25g/kg的靈芝固態培養基為作為白蝦免疫激活物的最適添加量。
zh_TW
dc.description.abstractThe study purpose to evaluate the effect of Ganoderma lucidum solid medium on growth performance and non-specific immune response of white shrimp (Litopenaeus vannamei).The shrimp diets was supplemented with different levels of Ganoderma lucidum solid medium (0g/kg , 7.5g/kg , 12.5g/kg , 25g/kg , 50g/kg)respectively. In growth performance test,5 groups of shrimp was fed for 6 weeks . The results show that all groups have no significant on Ganoderma lucidum solid medium supply,but 50g/kg supply have low survival than other groups. In immunity test, prophenoloxidase activity (proPO),phagocytic activity,superoxide anion production (o2-),and lysozyme activity were examined after feeding for 0,4,7,10,14 days. The results indicated that 12.5g/kg supply with Ganoderma lucidum solid medium have the best superoxide anion production (o2-),and lysozyme activity than other treatments. On the other hand, The prophenoloxidase activity and phagocytic activity were significantly higher when shrimp fed with 12.5g/kg、50g/kg supply of Ganoderma lucidum solid medium.
The present study confirm that supplemented with Ganoderma lucidum solid medium could elevate non-specific immune index of white shrimp. Further, the optimal ratio of Ganoderma lucidum solid medium as immunostimulants to feed white shrimp
is 12.5~25g/kg.
en
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en
dc.description.tableofcontents圖表目錄 III
中文摘要 IV
Abstract V
壹 文獻整理 1
一.蝦種介紹 1
二.產業歷史與現況 2
三.蝦類免疫 3
四.免疫激活物(Immunostimulants) 10
五.靈芝 11
六.研究動機與目的 12
貳 材料與方法 13
實驗動物與材料 13
實驗設計 13
成長分析 14
免疫分析 14
參 結果 18
一.成長表現 18
二.原酚氧化酵素活性(Prophenoloxidase activity,PO activity) 18
三.吞噬活性(Phagocytic activity) 18
四.超氧陰離子(Superoxide anion, O2-)產生量 19
五.溶菌酶活性(Lysozyme activity) 19
肆 討論 20
一.成長表現 20
二.免疫指標 20
伍 結論 24
陸 參考文獻 25
dc.language.isozh-TW
dc.subject南美白蝦zh_TW
dc.subject靈芝zh_TW
dc.subject固態培養基zh_TW
dc.subject免疫zh_TW
dc.subject成長zh_TW
dc.subjectGanoderma lucidum solid mediumen
dc.subjectLitopenaeus vannameien
dc.subjectgrowthen
dc.subjectinnate immune responseen
dc.title飼料中添加不同比例之靈芝固態培養基對南美白蝦成長表現及非特異性免疫之影響zh_TW
dc.titleEffects of dietary different levels of Ganoderma lucidum solid medium on the growth and innate immune response of White shrimp (Litopenaeus vannamei)en
dc.typeThesis
dc.date.schoolyear103-2
dc.description.degree碩士
dc.contributor.coadvisor陳秀男
dc.contributor.oralexamcommittee劉富光,王永松
dc.subject.keyword靈芝,固態培養基,南美白蝦,成長,免疫,zh_TW
dc.subject.keywordGanoderma lucidum solid medium,Litopenaeus vannamei,growth,innate immune response,en
dc.relation.page50
dc.rights.note有償授權
dc.date.accepted2015-08-10
dc.contributor.author-college生命科學院zh_TW
dc.contributor.author-dept漁業科學研究所zh_TW
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