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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林?輝(Feng-Huei Lin) | |
| dc.contributor.author | Fan-Qi Meng | en |
| dc.contributor.author | 孟繁琦 | zh_TW |
| dc.date.accessioned | 2021-06-15T16:29:00Z | - |
| dc.date.available | 2020-08-25 | |
| dc.date.copyright | 2015-08-25 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-13 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52817 | - |
| dc.description.abstract | 退化性關節炎是種相當常見的疾病，會嚴重的影響患者的活動行為及生活品質。目前對於早期的退化性膝關節炎治療方式為注射透明質酸 (HA) 或服用止痛藥，病情嚴重時必須進行人工關節置換手術。透明質酸注射治療是將透明質酸注射進膝關節腔，可以增加滑液囊中的潤滑液，減少關節互相摩擦，達到減輕病人疼痛、延緩退化症狀惡化的效果，在臨床上廣泛應用多年。但因為注射進入的透明質酸會被關節腔內的透明質酸酶和自由基分解，故治療效果短。病人需要接受多次注射治療，在醫療物資及金錢上都造成了相當的損耗。 在以往的文獻指出氧化鈰奈米顆粒 (CeO2) 具有消除自由基、抗发炎的功能，故本研就運用氧化鈰奈米顆粒結合透明質酸，期望可以预防早期退化性关节炎。 本研究運用金屬鹽水解法在常溫下製備氧化鈰納米顆粒，進行如下分析1.氧化鈰納米顆粒物性分析。2.氧化鈰納米顆粒消除自由基功能分析3.氧化鈰納米顆粒體外測試。4. 試驗檢測氧化鈰納米顆粒對初級培養豬軟骨細胞的預防效果。 本研究結果顯示以金屬鹽水解法成功製備出氧化鈰納米顆粒，其在細胞培養基中粒徑為120 nm左右，具有良好的分散性。氧化鈰納米顆粒濃度在6.7 ng CeO2/1x104細胞具有良好的生物相容性。運用過氧化氫的濃度0.03 mmol/1x104細胞處理初級培養豬軟骨細胞30分鐘後可以建立初期退化性關節炎的損傷模型。當初級培養豬軟骨細胞經過氧化鈰納米顆粒/透明質酸共同培養一天後，進行初期退化性關節炎的損傷模型實驗，發現該處理可以減少過氧化氫對初級培養豬軟骨細胞的損傷。 由研究結果得知氧化鈰納米顆粒/透明質酸對於早期退化性關節炎的预防具有潛在性的臨床運用價值。 | zh_TW |
| dc.description.abstract | Osteoarthritis (OA) is the most common type of joint disease, accompanied by varying degrees of functional limitation, reducing life quality of patients, especially knee osteoarthritis. Hyaluronic acid (HA) joint injections and pain relievers are efficient treatments for early stage osteoarthritis of the knee whereas patients have to knee replacement surgery in the later stages of osteoarthritis. After injected directly into the cavity around the knee joint, HA works by acting like a lubricant and shock absorber in the joints, eases the pain and helps the joints to work properly. However, for the decomposition by hyaluronidase and free radicals in knee joint, HA injection treatment has limited effect time. HA injection shows low benefits to patients suffer from knee and joint osteoarthritis. The cerium oxide nanoparticles (CNPs) has been reported that a long time free radical scavenger. CNPs combined with HA expected that extending HA decomposition time and having positive effect to osteoarthritis therapy. CNPs were synthesis by salt hydrolysis at room temperature. This study has divided four parts: 1. to analyze for physical property of CNPs. 2. To exam the free radicals are removed by CNPs. 3. The in vitro assays of CNPs. 4. To exam the CNPs /HA for early protection of chondrocyte. CNPs had successful synthesized by salt hydrolysis with particle size about 120 nm, showed good dispersibility in culture medium and good antioxidant ability. CNPs are biocompatible under the ratio of 6.7 ng/ 1x104 cells. The OA model can be established by the cell treated with H2O2 for 30 min, which ratio to 1x104 cells is 0.03 mmol. The H2O2 damage effect could be resisted when chondrocyte cultured with CNPs /HA after 1 day. The results suggest that CNPs /HA would be a potential candidate for early stage osteoarthritis treatment. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T16:29:00Z (GMT). No. of bitstreams: 1 ntu-104-R02527068-1.pdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 審定書 i 誌 謝 ii 摘 要 iii Abstract v 圖目錄 ix 表目錄 xi 縮 寫 xii 第 1 章 緒 論 1 1.1 研究背景 1 1.2 退化性關節炎簡介 1 1.3 退化性關節炎的治療 4 1.4 研究目的 5 第 2 章 理論基礎 6 2.1 氧化鈰簡介 6 2.1.1 氧化鈰的結構與性質 6 2.1.2 氧化鈰的應用 7 2.1.3 氧化鈰的製備 14 2.2 透明質酸 16 第 3 章 實驗方法 17 3.1 實驗儀器 17 3.2 實驗藥品 18 3.3 試驗方法及流程 19 3.4 氧化鈰納米顆粒的合成 19 3.5 氧化鈰納米顆粒的分析 20 3.5.1 形貌表徵 20 3.5.2 成分表徵 21 3.5.3 抗氧化性能測試 21 3.6 軟骨細胞培養 23 3.6.1 培養基的配製 23 3.6.2 磷酸鹽緩衝溶液的配製 23 3.6.3 軟骨細胞獲取和培養 23 3.7 生物相容性測試 24 3.7.1 WST-1細胞增生及活性測試 24 3.7.2 LDH 細胞毒性測試 26 3.7.3 氧化鈰納米顆粒細胞相容性測試 28 3.8 過氧化氫誘導退化性關節炎初期的軟骨細胞受損模型的建立 28 3.8.1 live/dead細胞活性毒性檢測 28 3.8.2 細胞實驗 30 3.9 細胞凋亡實驗 31 3.9.1 annexinⅤ-FITC / PI染色 31 3.9.2 實驗設計 32 3.10 即時螢光定量PCR 34 3.11 阿爾新藍染色 35 第 4 章 結果與討論 37 4.1 納米氧化鈰表徵 37 4.1.1 形貌分析 37 4.1.2 成分分析 39 4.1.3 抗氧化能力測定 40 4.1.4 粘度測試 41 4.2 過氧化氫誘導退化性關節炎初期的軟骨細胞受損模型的建立 42 4.3 納米氧化鈰顆粒在軟骨細胞中的生物相容性 44 4.4 細胞凋亡實驗 45 4.5 基因表現 49 4.6 GAG 染色 51 第 5 章 結論 59 第 6 章 参考文献 60 | |
| dc.language.iso | zh-TW | |
| dc.subject | 納米氧化鈰 | zh_TW |
| dc.subject | 退化性關節炎 | zh_TW |
| dc.subject | 自由基 | zh_TW |
| dc.subject | 透明質酸 | zh_TW |
| dc.subject | hyaluronic acid | en |
| dc.subject | free radical | en |
| dc.subject | osteoarthritis | en |
| dc.subject | cerium oxide nanoparticles | en |
| dc.title | 含氧化鈰抗氧化劑的透明質酸水凝膠於退化性關節炎的治療 | zh_TW |
| dc.title | The study of cerium oxide nanoparticles antioxidant and hyaluronic acid hydrogel for osteoarthritis treatment | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 楊禎明(Jen-Ming Yang),郭士民(Shyh-Ming Kuo),張淑真(Shwu-Jen Chang) | |
| dc.subject.keyword | 退化性關節炎,納米氧化鈰,自由基,透明質酸, | zh_TW |
| dc.subject.keyword | osteoarthritis,cerium oxide nanoparticles,free radical,hyaluronic acid, | en |
| dc.relation.page | 64 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-08-14 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 材料科學與工程學研究所 | zh_TW |
| 顯示於系所單位： | 材料科學與工程學系 | |
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