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DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李芳仁 | |
dc.contributor.author | Kang-Yu Peng | en |
dc.contributor.author | 彭康禹 | zh_TW |
dc.date.accessioned | 2021-06-15T16:26:15Z | - |
dc.date.available | 2020-09-25 | |
dc.date.copyright | 2015-09-25 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-08-14 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52757 | - |
dc.description.abstract | 酵母菌核醣核酸結合蛋白Rbp1最初被發現為會負向調控生長的因子。其在結構上包含三個核醣核酸識別序列(RRM)和兩個富含麩氨酸區域,並且在碳端具有一個富含天門冬氨酸、甲硫胺酸和脯胺酸的區域。實驗室之前研究已經發現在 | zh_TW |
dc.description.abstract | Rbp1, as a RNA binding protein, was first identified as a negative growth regulator in Saccharomyces cerevisiae. Rbp1 contains three RNA recognition motif (RRM), two glutamine-rich regions, and asparagine-methionine-proline-rich (NMP) region in the C terminus. Our previous studies have shown that deletion of RBP1 resulting in a hyper ager-invasive growth in | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T16:26:15Z (GMT). No. of bitstreams: 1 ntu-104-R02448003-1.pdf: 1735990 bytes, checksum: f9b577dcbd54ad5b47940abb3ea26286 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 論文口試委員會審定書 I 致謝 II 中文摘要 III Abstract IV Table of Contents VI Introduction 1 Materials and Methods 10 Results 21 Part I. To investigate the role of Rbp1 in the regulation of FLO11 transcription 21 Part II. Regulation of T637 phosphorylation of Rbp1 in yeast 28 Discussion 30 Figures Figure 1. mRNA expression level of FLO8, MSS11,and STE12 remain unchanged compared to FLO11 in response to YP treatment. 35 Figure 2. Mss11 and Flo8 protein expression level increase in rbp1D mutant. 36 Figure 3. Translation activity of invasion-related genes increase in rbp1D mutant in log phase. 37 Figure 4. Translation activity of invasion-related genes increase in rbp1D mutant in response to glucose deprivation. 38 Figure 5. Quantification of the distribution of invasion-related mRNAs in ribosome-polysome profile. 39 Figure 6. Rbp1 can bind to invasion-related mRNAs. 40 Figure 7. Rbp1 can also bind to invasion-related mRNAs in BY4741. 41 Figure 8. Rbp1 can not interact with eIF4G2-full length. 42 Figure 9. eIF4G2 is involved in rbp1D-induced highly invasive growth. 43 Figure 10. Expression of eIF4G2p also increases in rbp1D mutants. 44 Figure 11. Anti-T637P antibody can specific recognize non-T637 mutation containing protein. 45 Figure 12. T637 phosphorylation decreases in response to glucose deprivation. 46 Figure 13. Rbp1 still has its function in regulation of invasive growth regardless of T637 phosphorylation state. 47 Supplementary Figures Figure S1. Dhh1 can interact with eIF4G2-full length. 48 Figure S2. HA-tag in construct of MSS11,FLO8,and STE12 in S1278b strain.. 49 Appendixes Appendix . The complex regulation of the FLO11 promoter by conventional and epigenetic mechanisms. 50 Tables Table 1. Yeast strains used in this study 51 Table 2. Primers used in this study 53 Table 3. Plasmids used in this study 57 Table 4. Antibodies used in this study 59 Reference 61 | |
dc.language.iso | en | |
dc.title | 探討酵母菌核糖核酸結合蛋白Rbp1p對絲狀生長中多種基因之轉譯調控 | zh_TW |
dc.title | Characterization of Rbp1p-mediated translation of variegated genes in yeast filamentous growth | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 羅?升,林敬哲,鄧述諄 | |
dc.subject.keyword | 核糖核酸結合蛋白,酵母菌,轉譯,菌絲生長,轉錄因子, | zh_TW |
dc.subject.keyword | Rbp1,yeast,translation,filamentous growth,transcription factor, | en |
dc.relation.page | 65 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2015-08-14 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 分子醫學研究所 | zh_TW |
顯示於系所單位: | 分子醫學研究所 |
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