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  1. NTU Theses and Dissertations Repository
  2. 生物資源暨農學院
  3. 動物科學技術學系
請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52502
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dc.contributor.advisor林美峰(Mei-Fong Lin)
dc.contributor.authorPei-Hsuan Chenen
dc.contributor.author陳姵璇zh_TW
dc.date.accessioned2021-06-15T16:16:41Z-
dc.date.available2018-08-25
dc.date.copyright2015-08-25
dc.date.issued2015
dc.date.submitted2015-08-17
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dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52502-
dc.description.abstract本試驗之目的係在探究白色發光二極體(white light-emitting diode, 白光 LED)作為產蛋鴨之室內人工光源時,對褐色菜鴨產蛋性能和鈣利用之影響。試驗使用144隻褐色菜鴨,並逢機分配給3個不同光源組合之處理組,分別為螢光燈加日光(FS)、白光LED加日光(LS)以及白光LED(WL)。每日光照時數為16個小時,水與飼料為任食;每日記錄室內溫溼度以及個別產蛋表現,自18週齡開始,每2週會連續3天收集當日產蛋並測定蛋品質;於41週齡時,每處理組挑選12隻穩定產蛋之菜鴨用於鈣利用率試驗,並在43週齡將其犧牲,收集腸道、殼腺組織和左腳脛骨供分析檢測之用。結果顯示,FS處理組較快達到產蛋高峰,但整體產蛋率於各組間並無顯著差異。除FS處理組在少數幾週出現顯著或趨勢性較高的蛋重、LS處理組在最後2週出現顯著較薄的蛋殼厚度,以及LS與WL處理組於最後1週出現顯著較低之蛋殼強度外,其他蛋品質性狀於大部分試驗期間在3個處理組間皆無顯著差異。WL處理組有較少的蛋殼沉積鈣量,且有較高的骨灰分比率,而鈣結合蛋白於WL處理組在殼腺內亦有較低的表現量,唯與其他兩處理組間未達差異顯著。利用免疫組織化學染色法來測定鈣結合蛋白的分布位置,結果顯示腸道及殼腺之鈣結合蛋白分布於細胞質內,且停產菜鴨有較少鈣結合蛋白之分布。就能源效益而言,比較耗電量與成本計算,發現使用白光LED取代螢光燈管,每支燈管每個月可節省12元。總結而論,於試驗期間,白光LED對於蛋品質以及鈣利用上沒有顯著的負面影響,且由於LED的低耗能,用白光LED作為室內飼養光源將是一個不錯的選擇。然而,長期之影響仍須進一步試驗來印證之。zh_TW
dc.description.abstractThe objective of this study was to understand the effect of white light emitting diode(LED)as the source of artificial lights on egg performance and calcium utilization of Brown Tsaiya duck. A total of 144 birds were used in this study and divided randomly into 3 groups for the following treatments: white fluorescent tube with sunlight(FS), white LED with sunlight(LS),and white LED(WL). The light was shone for 16 hours a day, and the ambient temperature and humidity was recorded daily. Eggs were collected for 3 continuous days every 2 weeks to determine the egg quality. Twelve consistent laying ducks per treatment were chosen for calcium utilization test at 41 week of age and sacrificed at 43 week of age. The quality of left tibia bone, and the expression and localization of calbindin in duodenum and shell gland were also determined. There was no significant difference in egg quality except that the egg weight of FL treatment tended to be higher than the other treatments in few weeks, and LS treatment had lower shell thickness in the last 2 weeks. WL treatment also had a lower trend of calbindin expression in shell gland and calcium deposition of shell, but had potentially higher bone ash percentage. The localization of calbindin in duodenum and shell gland was observed to be distributed in the cytoplasm, and less calbindin distribution of stop-laying ducks compared with normal-laying ducks. In addition to the above, LED was able to save 12 NT dollars per month per tube compared with fluorescent tube. In conclusion, during the trial period, white LED had no significant negative effect on egg quality and calcium utilization. Furthermore, LED was more efficient and resulted in lowering total cost. It can therefore be a good and alternative light source for indoor rearing.en
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dc.description.tableofcontents謝 誌 I
摘 要 II
ABSTRACT III
目 錄 IV
圖 次 VII
表 次 VIII
緒 言 1
壹、 文獻檢討 2
一、 不同光源之比較 2
(一)、 常用人工光源簡介 2
(二)、 各光源之耗能性 5
(三)、 各光源之波長 6
(四)、 各光源之紫外線 8
二、 家禽對於紫外線與不同波長的敏感度 9
三、 光源對鈣吸收之重要性 10
(一)、 維生素D合成與代謝機制 10
(二)、 1,25-(OH)2D3之調控作用 12
(三)、 維生素D相關蛋白質 12
(四)、 維生素D對鈣吸收之調控 13
四、 鈣代謝機制 14
(一)、 鈣的吸收 14
(二)、 鈣的儲存 15
(三)、 蛋殼合成 16
五、 鈣結合蛋白 18
(一)、 鈣結合蛋白的構造與分布位置 18
(二)、 鈣結合蛋白的功能 20
(三)、 調控因子 20
(四)、 與蛋殼品質之關係 22
貳、 材料與方法 23
一、 試驗動物 23
二、 試驗環境 23
(一)、 室內溫濕度 23
(二)、 室內光照 23
三、 試驗設計 26
(一)、 鈣消化率試驗 27
(二)、 鈣利用率試驗 27
四、 分析方法 29
(一)、 蛋品質分析 29
(二)、 營養分析 30
(三)、 骨質強度分析 34
(四)、 組織染色 35
(五)、 蛋白質電泳分析 38
五、 統計分析 42
參、 結果 43
一、 飼糧營養成分 43
二、 產蛋表現與蛋品質 44
(一)、 產蛋率 44
(二)、 蛋品質 45
三、 骨質測定 49
四、 鈣利用試驗 50
(一)、 鈣利用率 50
(二)、 鈣消化率 50
五、 鈣結合蛋白(28kDa) 51
(一)、 表現量 51
(二)、 分布位置 52
(三)、 停產之影響 53
六、 耗能 55
肆、 討論 56
一、 光源對產蛋表現之影響 56
(一)、 產蛋率 56
(二)、 蛋品質 58
二、 光源對鈣利用之影響 60
三、 能量以及成本計算 62
伍、 結論 63
參考文獻 64
附 錄 74
小 傳 76
dc.language.isozh-TW
dc.subject發光二極體zh_TW
dc.subject褐色菜鴨zh_TW
dc.subject維生素D3zh_TW
dc.subject鈣zh_TW
dc.subject蛋品質zh_TW
dc.subjectcalciumen
dc.subjectlight-emitting diodeen
dc.subjectvitaminD3en
dc.subjectBrown Tsaiya ducken
dc.subjectegg qualityen
dc.title白色發光二極體(LED)對褐色菜鴨產蛋表現與鈣利用之影響zh_TW
dc.titleThe effect of white light-emitting diode (LED) on laying performance and calcium utilization in Brown Tsaiya ducken
dc.typeThesis
dc.date.schoolyear103-2
dc.description.degree碩士
dc.contributor.oralexamcommittee黃士哲,黃振芳,魏恆巍
dc.subject.keyword發光二極體,褐色菜鴨,維生素D3,鈣,蛋品質,zh_TW
dc.subject.keywordlight-emitting diode,Brown Tsaiya duck,calcium,vitaminD3,egg quality,en
dc.relation.page76
dc.rights.note有償授權
dc.date.accepted2015-08-17
dc.contributor.author-college生物資源暨農學院zh_TW
dc.contributor.author-dept動物科學技術學研究所zh_TW
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