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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王勝仕(Sheng-Shih Wang) | |
dc.contributor.author | Jen-Wei Chen | en |
dc.contributor.author | 陳仁偉 | zh_TW |
dc.date.accessioned | 2021-06-15T16:16:06Z | - |
dc.date.available | 2020-08-28 | |
dc.date.copyright | 2015-08-28 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-08-17 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52484 | - |
dc.description.abstract | 本研究旨在探討不同hydroxyethyl methacrylate (HEMA)與methacrylic acid (MAA)組成比例對於其共聚物水膠特性之影響,本研究中一共聚合三種不同重量百分比之HEMA:MAA單體溶液(98.8:0、96.8:2、93.8:4.9),經由UV光照射聚合後形成水膠。本研究中所探討之水膠特性包含:表面疏水性、飽和含水率、表面粗糙度、透光率,以及膠體在溶菌酶溶液中,對於溶菌酶之吸附和後續之脫附。自膠體脫附後的溶菌酶,其結構也以多種光譜及實驗方法檢驗。除此之外,格蘭氏陰性(大腸桿菌)及陽性菌(金黃色葡萄球菌)於膠體表面貼附之行為亦在本研究之探討範圍中。以HEMA-co-MAA水膠作為模型材料,我們希望得以觀察MAA之添加對於膠體特性之影響,並了解其中之機制。 | zh_TW |
dc.description.abstract | The purpose of this work was to investigate the relationship between the feed composition of 2-hydroxyethyl methacrylate (HEMA)/methacrylic acid (MAA) and the characteristics of the resultant hydrogel material. The monomer solutions of HEMA and MAA were mixed at different weight ratios of 98.8:0, 96.8:2, 93.8:4.9, 89:9.9, and 79.1:19.8, and were subjected to UV irradiation. Properties including surface hydrophobicity (by water contact angles), equilibrium water content (EWC), mean surface roughness (by AFM), and transparency (by UV/Vis-absorption spectrometer) were studied on these hydrogels. When the gel was soaked in DI water, the EWC was observed to be in the range of 30-40%. However, a significantly higher EWC (~60%) was perceived upon soaking in PBS. Our results indicated that the addition of hydrophilic MAA lead to a reduction in water contact angle, showing a negative correlation between the amount of MAA and surface hydrophobicity. Also, the addition of MAA was found to increase the mean surface roughness and surface energy, both are related to the decrease in hydrophobicity. Almost no difference was detected in the transparency of hydrogels soaked in DI water and PBS except for the latter exhibiting a rapid rise near the wavelength of 200 nm. In addition, the adsorption and desorption behaviors of a model protein, lysozyme, on the hydrogels were examined. The result indicated that the addition of MAA would increase lysozyme adsorption but the lysozyme desorption was decreased. We believe the result of this work may aid in the development and improvement of the materials used for soft contact lenses. | en |
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dc.description.tableofcontents | 目錄
誌謝 …………………………………………………………………… i 中文摘要 ……………………………………………………………… ii Abstract ……………………………………………………………… iii 目錄 …………………………………………………………………… iv 圖目錄 ………………………………………………………………… vii 表目錄 ………………………………………………………………… ix 第一章 緒論 …………………………………………………………… 1 第二章 文獻回顧 ……………………………………………………… 2 2.1 隱形眼鏡的分類 ………………………………………… 2 2.1.1 硬式隱形眼鏡 ……………………………… 2 2.1.2 軟式隱形眼鏡 ……………………………… 3 2.2 水膠性質 ………………………………………………… 5 2.2.1 飽和含水率 (Equilibrium Water Content) ……………………………………………………… 5 2.2.2 透氧率 ……………………………………… 7 2.2.3 表面親水性 ………………………………… 9 2.2.4 表面粗糙度 ……………………………… 12 2.3 蛋白質吸附 …………………………………………… 12 2.3.1 淚液之組成與結構 ……………………… 15 2.3.2 溶菌酶 …………………………………… 16 2.4 微生物附著 …………………………………………… 24 2.4.1 與隱形眼鏡相關之併發症 ……………… 24 2.4.2 微生物引起之角膜炎 (Microbial Keratitis, MK) ………………………………… 25 2.4.3 其他常見之併發症 ……………………… 25 2.4.4 細菌於隱形眼鏡上之貼附 ……………… 28 2.5 實驗測量技術 ………………………………………… 31 2.5.1 表面接觸角 ……………………………… 31 2.5.1.1 表面粗糙度對接觸角之影響 …………………………………………… 32 2.5.1.2 表面濕潤性與接觸角關係 … 34 2.5.1.3 前進角與後退角 …………… 35 2.5.1.4 液滴(氣泡)大小對接觸角之影響 …………………………………………… 38 2.5.2 表面自由能 ……………………………… 41 2.5.2.1 狀態方程式 ………………… 41 2.5.2.2 表面自由能之拆解 ………… 42 2.5.2.3 計算表面自由能之方法 …… 43 2.5.3 原子力顯微鏡 (Atomic Force Microscope, AFM) ……………………………………………… 49 第三章 研究動機 …………………………………………………… 58 第四章 實驗藥品、儀器與流程 …………………………………… 59 4.1 實驗藥品 ……………………………………………… 59 4.2 實驗儀器 ……………………………………………… 59 4.3 實驗步驟 ……………………………………………… 60 4.3.1 PDMS模具製作 …………………………… 60 4.3.2 HEMA/MAA水膠製作 ……………………… 60 4.3.3 飽和含水率測量 ………………………… 61 4.3.4 表面接觸角測量 ………………………… 61 4.3.5 透光率測量 ……………………………… 61 4.3.6 表面粗糙度測量 ………………………… 62 4.3.7 溶菌酶吸附與脫附 ……………………… 62 4.3.8 脫附溶菌酶之結構分析 ………………… 62 4.3.8.1 蛋白質自身螢光 …………… 62 4.3.8.2 ANS染劑螢光 ………………… 62 4.3.8.3 圓二色光譜 ………………… 63 4.3.8.4 溶菌酶活性測量 …………… 63 4.3.9 細菌附著 ………………………………… 63 4.3.9.1 滅菌 ………………………… 63 4.3.9.2 活化及培養 ………………… 64 4.3.9.3 在水膠上之附著 …………… 64 4.3.9.4 spread plate count ……… 64 第五章 實驗結果與討論 …………………………………………… 65 5.1 飽和含水率 (EWC) …………………………………… 65 5.2 表面接觸角 …………………………………………… 68 5.3 透光率測量 …………………………………………… 69 5.4 表面粗糙度 …………………………………………… 72 5.5 細菌貼附 ……………………………………………… 77 5.6 溶菌酶吸/脫附 ………………………………………… 79 5.7 自膠體表面脫附溶菌酶之結構 ……………………… 82 5.7.1 螢光光譜 ………………………………… 82 5.7.2 圓二色光譜 ……………………………… 82 5.7.3 溶菌酶活性 ……………………………… 83 第六章 結論與建議 ………………………………………………… 88 參考文獻 ……………………………………………………………… 89 圖目錄 圖2.1.1. 氧氣在水膠(紅)與矽水膠(藍)鏡片內傳遞之濃度對距離作圖 ………………………………………………………………………… 4 圖2.2.1. 水膠(○)與矽水膠(●)之含水率與透氧率關係圖 ……… 6 圖2.2.2 淚液分布示意圖 …………………………………………… 10 圖2.2.3 高分子水膠結構示意圖 …………………………………… 11 圖2.2.4 表面粗糙度對接觸角之影響 ……………………………… 13 圖2.3.1 水膠結構示意圖 …………………………………………… 14 圖2.3.2 淚液結構示意圖 …………………………………………… 15 圖2.3.3 溶菌酶活性區域D催化反應機制 …………………………… 17 圖2.4.1 綠膿桿菌之adhesion與宿主細胞之receptor結合 ……… 30 圖2.5.1 水與表面接觸角之示意圖 ………………………………… 31 圖2.5.2 界面移動示意圖 …………………………………………… 33 圖2.5.3 表面粗糙度r與接觸角θ關係 ……………………………… 33 圖2.5.4 表面(不)濕潤現象 ………………………………………… 34 圖2.5.5 以座滴法量測動態接觸角(一) …………………………… 35 圖2.5.6 以座滴法量測動態接觸角(二) …………………………… 36 圖2.5.7 以貼泡法量測動態接觸角 ………………………………… 37 圖2.5.8 以威氏平板法量測動態接觸角 …………………………… 39 圖2.5.9 威勢平板法裝置示意圖 …………………………………… 40 圖2.5.10 液滴半徑與液滴形狀關係 ………………………………… 40 圖2.5.11. 低密度聚乙烯之臨界表面自由能 ……………………… 45 圖2.5.12 樣品表面汙染層示意圖 …………………………………… 49 圖2.5.13 不同探針尺寸與樣品表面之互動 ………………………… 50 圖2.5.14 探針受力與表面距離關係 ………………………………… 50 圖2.5.15 雷射光訊號接收器 ………………………………………… 51 圖2.5.16 壓電材料之形變與電壓關係 ……………………………… 51 圖2.5.17 壓電材料之震盪 …………………………………………… 52 圖2.5.18 AFM操作模式 ……………………………………………… 53 圖2.5.19 AFM於溶液環境中之操作 ………………………………… 54 圖2.5.20 探針之受力 ………………………………………………… 54 圖2.5.21 接觸模式對樣品之損害 …………………………………… 55 圖2.5.22 Tapping mode示意圖 ……………………………………… 56 圖2.5.23 材質差異造成相位差 ……………………………………… 56 圖2.5.24 地形圖(左)與相位差圖(右) ……………………………… 57 圖5.1 膠體含水率與時間關係 ……………………………………… 66 圖5.2 於去離子水中膨潤水膠之透光率 …………………………… 70 圖5.3 於PBS中膨潤水膠之光率 ……………………………………… 71 圖5.4 日拋型軟式隱形眼鏡之紫外光穿透光譜圖 ………………… 71 圖5.5 水膠之表面地形圖 …………………………………………… 73 圖5.6 不同狀態與環境中膠體表面地形圖(左:乾燥狀態,大氣環境;右:飽和含水,溶液環境) …………………………………………… 75 圖5.7 膠體表面結構示意圖 ………………………………………… 76 圖5.8 細菌之貼附量 ………………………………………………… 78 圖5.9 溶菌酶吸附曲線 ……………………………………………… 80 圖5.10 溶菌酶脫附曲線 ……………………………………………… 81 圖5.11 脫附溶菌酶自身螢光 ………………………………………… 84 圖5.12 脫附溶菌酶ANS螢光 ………………………………………… 85 圖5.13 脫附溶菌酶二級結構 ………………………………………… 86 圖5.14 脫附溶菌酶之活性 …………………………………………… 87 表目錄 表 2.2.1. FDA制定之軟式隱形眼鏡 ………………………………… 6 表2.2.2 市面上常見之軟式隱形眼鏡之含水率與透氧率 …………… 8 表2.3.1 淚液成分 …………………………………………………… 14 表2.3.2 淚液組成 …………………………………………………… 18 表2.3.3 淚液中蛋白質之位置與功能 ……………………………… 20 表2.4.1 與隱形眼鏡相關之併發症 ………………………………… 26 表2.4.2 不同菌種引發角膜炎之機率 ……………………………… 27 表2.4.3 不同微生物可能引起之併發症 …………………………… 28 表2.5.1 常見高分子之表面能 ……………………………………… 47 表4.2.1實驗儀器種類、型號與製造商列表 ………………………… 59 表5.1 飽和含水率 …………………………………………………… 65 表5.2 不同共聚物/組成之飽和含水率 ……………………………… 66 表5.3 表面接觸角 …………………………………………………… 68 表5.4 HEMA-co-MAA水膠之表面自由能 ……………………………… 68 表5.5 不同高分子與水之接觸角與表面自由能 …………………… 69 表5.6 水膠之方均根表面粗糙度 …………………………………… 72 表5.7 市面上常見隱形眼鏡材料之表面粗糙度 …………………… 74 表5.8 溶菌酶之吸附量、脫附量與脫附比率 ……………………… 79 | |
dc.language.iso | zh-TW | |
dc.title | 探討甲基丙烯酸羥乙酯與甲基丙烯酸共聚物水膠作為隱形眼鏡材料之特性 | zh_TW |
dc.title | Characterization of poly-(hydroxyethyl methacrylate-co-methacrylic acid) Hydrogel as Contact Lens Material | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 廖思婷,吳宛儒,賴進此,王孟菊,林達顯 | |
dc.subject.keyword | 隱形眼鏡,水膠,HEMA,MAA,溶菌?, | zh_TW |
dc.subject.keyword | Hydrogel,2-hydroxyethyl methacrylate,methacrylic acid,contact lens,lysozyme, | en |
dc.relation.page | 93 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2015-08-17 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
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