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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52459完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李伯訓 | |
| dc.contributor.author | Yi-Ting Chen | en |
| dc.contributor.author | 陳伊婷 | zh_TW |
| dc.date.accessioned | 2021-06-15T16:15:23Z | - |
| dc.date.available | 2020-09-24 | |
| dc.date.copyright | 2015-09-24 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-17 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52459 | - |
| dc.description.abstract | 聚甲基丙烯酸甲酯主要應用於牙科矯正維持器及假牙基底樹酯，因口內微生物環境複雜，易使微生物貼附於聚甲基丙烯酸甲酯形成生物膜，造成齲齒及牙周病，故其表面的改質為學者們所致力的目標。我們發現將2-甲基丙烯酸羥基乙酯以熱處理方式接枝於聚甲基丙烯酸甲酯可抗細菌貼附且具生物相容性，但其抗貼附機制和真正應用於人類口腔內是否有效仍不明確。 本論文我們將藉由薄膜界面電位分析儀和胞外多醣體酚-硫酸法探討聚甲基丙烯酸甲酯(PMMA)以及表面接枝2-甲基丙烯酸羥基乙酯之聚甲基丙烯酸甲酯(PMMA-HEMA)表面電位影響細菌貼附情形以及細菌貼附於表面上所必需分泌的胞外多醣體多寡，並且也將PMMA和PMMA-HEMA進行臨床試驗，藉由共軛焦顯微鏡和電子顯微鏡去觀察口腔內細菌貼附於試片表面上的情況以及活菌和死菌的比例。 結果顯示PMMA-HEMA的表面電位比PMMA較為帶負電，且因細菌的胞外膜為負電，能使其表面與細菌外膜有相斥的作用。此外經過細菌培養後萃取PMMA-HEMA表面細菌所分泌的胞外多醣體，測得的量也微乎其微。顯示其表面可抗細菌貼附而無胞外多醣體沾黏於表面上，所以細菌無法彼此聚集形成生物膜。臨床試驗中也發現PMMA-HEMA表面的細菌貼附量比PMMA少，故使用熱處理接枝HEMA於PMMA表面上應用於人體口腔內仍有抗細菌貼附的效果。 | zh_TW |
| dc.description.abstract | Polymethyl methacrylate (PMMA) is mainly used in orthodontic removable retainer and denture base resin. Because intraoral microorganism environment is complex, microorganisms easily attach to PMMA and form biofilm. Uncontrolled accumulation of bacteria and fungal biofilms on or surrounding dental devices may contribute to dental caries and periodontal disease. Surface modification on polymeric materials and control of microbial infections are a very important issue in modern society. We found that 2-hydroxyethyl methacrylate (HEMA) grafted onto heat-treated PMMA could decrease bacteria adhesion and possess biocompatibility. However, it is still unclear about PMMA-HEMA’s antiadhesion mechanism and application in human oral cavity. In this study, the zeta potential of PMMA and PMMA-HEMA surfaces and extracellular polysaccharides produced by bacteria were evaluated by electro-kinetic analyzer (EKA) and phenol-sulfuric acid method. Furthermore, the PMMA and PMMA-HEMA antibacterial adhesion clinical trial was observed by confocal laser scanning microscope and scanning electron microscopy. We analyzed the circumstances of oral bacterial attachment as well as the proportion of live bacteria and dead bacteria. Results of the PMMA and PMMA-HEMA surface zeta potential showed that PMMA-HEMA carried more negative charge compared to PMMA. Therefore, PMMA-HEMA exhibited ability to repel bacterium which had negative charge on cell wall. Moreover, we measured scanty extracellular polysaccharides on PMMA-HEMA surface which demonstrated that PMMA-HEMA inhibited biofilm formatin by antiadhesion of bacterial extracellular polysaccharides. In clinical trial, PMMA-HEMA prevented the adhesion of oral bacterium than PMMA. Therefore, the heat-treated PMMA-HEMA has antibiofilm activity and exhibits a potent broad spectrum antibacterial activity against salivary bacteria. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T16:15:23Z (GMT). No. of bitstreams: 1 ntu-104-R02450005-1.pdf: 3180764 bytes, checksum: 5816ced6bfd10f2691af33cc3de391fa (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 摘要 i Abstract ii 目錄 iv 圖目錄 viii 表目錄 xi 1 第一章 前言 1 2 第二章 文獻回顧 2 2.1 聚甲基丙烯酸甲酯(PMMA) 2 2.1.1 聚甲基丙烯酸甲酯在生物醫材及牙科的應用 2 2.1.2 聚甲基丙烯酸甲酯缺點 3 2.2 生物膜（biofilm） 4 2.2.1 生物膜的定義 4 2.2.2 生物膜的形成 4 2.3 2-甲基丙烯酸羥基乙酯(HEMA) 5 2.4 唾液(saliva)與細菌貼附之作用 6 2.5 聚合物抗菌機制 8 2.6 細菌之胞外多醣體(bacterial EPS) 9 2.7 醫療器材生物相容性評估 11 2.7.1 評估局部與全身風險 11 2.7.2 體外細胞毒性(cell cultures for toxicity testing) 12 2.7.3 致敏性 14 2.7.4 刺激性 14 2.8 臨床試驗 15 3 第三章 實驗動機與目的 18 3.1 研究動機 18 3.2 研究目的 18 4 第四章 實驗材料與方法 19 4.1 實驗材料 19 4.2 實驗儀器 28 4.3 實驗流程圖 30 4.4 試片製作 31 4.4.1 製備聚甲基丙烯酸甲酯(PMMA) 31 4.4.2 試片接枝(HEMA、PEGMA) 31 4.5 細菌實驗 33 4.5.1 菌株特性、來源資料與培養保存方法 33 4.5.2 細菌貼附實驗 34 4.5.3 Bradford蛋白質分析法 35 4.6 細胞實驗 36 4.6.1 細胞解凍、培養、計數與保存方法 36 4.6.2 細胞存活率分析(MTT assay) 38 4.7 表面電位分析 41 4.8 胞外多醣體分析 41 4.8.1 胞外多醣萃取 41 4.8.2 總醣測定 42 4.8.3 傅立葉轉換紅外線光譜儀(FT-IR) 43 4.9 臨床前有毒物質釋放分析及動物試驗 43 4.9.1 傅立葉轉換紅外線光譜儀(FT-IR) 43 4.9.2 天竺鼠皮膚敏感性試驗(極大化法) 44 4.9.3 倉鼠口腔黏膜刺激試驗 47 4.10 臨床試驗 50 4.10.1 研究目的 50 4.10.2 個人維持器製作 51 4.10.3 雷射共軛焦顯微鏡(CLSM) 51 4.10.4 場發射掃描式電子顯微鏡(FE-SEM) 52 4.11 統計分析 53 5 第五章 實驗結果 54 5.1 細菌實驗 54 5.1.1 蛋白質貼附之細菌實驗 54 5.1.2 細菌貼附1、3、6小時實驗 56 5.2 表面電位分析(Zata Potential analysis) 59 5.3 胞外多醣體分析 60 5.4 細胞存活率分析(MTT assay) 63 5.5 臨床前有毒物質釋放分析及動物試驗 65 5.5.1 傅立葉轉換紅外線光譜儀(FT-IR) 65 5.5.2 天竺鼠皮膚敏感性試驗(極大化法) 65 5.5.1 倉鼠口腔黏膜刺激試驗 68 5.6 臨床試驗 72 5.6.1 個人維持器製作 72 5.6.2 雷射共軛焦顯微鏡(CLSM) 72 5.6.3 場發射掃描式電子顯微鏡(FE-SEM) 77 6 第六章 討論 80 6.1 細菌實驗 80 6.1.1 蛋白質貼附之細菌實驗 80 6.1.2 細菌貼附1、3、6小時實驗 80 6.2 表面電位分析 82 6.3 胞外多醣體分析 82 6.4 細胞存活率分析(MTT assay) 83 6.5 臨床前有毒物質釋放分析及動物試驗 84 6.5.1 傅立葉轉換紅外線光譜儀(FT-IR) 84 6.5.2 天竺鼠皮膚敏感性試驗(極大化法) 84 6.5.3 倉鼠口腔黏膜刺激試驗 85 6.6 臨床試驗 86 6.6.1 雷射共軛焦顯微鏡(CLSM) 86 6.6.2 場發射掃描式電子顯微鏡(FE-SEM) 87 7 第七章 結論 88 8 參考文獻 89 | |
| dc.language.iso | zh-TW | |
| dc.subject | 聚甲基丙烯酸甲酯 | zh_TW |
| dc.subject | 2-甲基丙烯酸羥基乙酯 | zh_TW |
| dc.subject | 表面電位 | zh_TW |
| dc.subject | 胞外多醣體 | zh_TW |
| dc.subject | 臨床試驗 | zh_TW |
| dc.subject | 2-hydroxyethyl methacrylate | en |
| dc.subject | polymethyl methacrylate | en |
| dc.subject | zeta potential | en |
| dc.subject | extracellular polysaccharides | en |
| dc.subject | clinical trial | en |
| dc.title | 2-甲基丙烯酸羥基乙酯表面接枝於聚甲基丙烯酸甲酯其抗菌貼附之機制研究暨臨床試驗 | zh_TW |
| dc.title | Mechanism and Clinical Trial of 2-Hydroxyethyl Methacrylate Grafting on Poly(methyl methacrylate) to Prevent Bacteria Adhesion | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃國皓,張哲政 | |
| dc.subject.keyword | 聚甲基丙烯酸甲酯,2-甲基丙烯酸羥基乙酯,表面電位,胞外多醣體,臨床試驗, | zh_TW |
| dc.subject.keyword | polymethyl methacrylate,2-hydroxyethyl methacrylate,zeta potential,extracellular polysaccharides,clinical trial, | en |
| dc.relation.page | 96 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-08-18 | |
| dc.contributor.author-college | 牙醫專業學院 | zh_TW |
| dc.contributor.author-dept | 口腔生物科學研究所 | zh_TW |
| 顯示於系所單位： | 口腔生物科學研究所 | |
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