請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52300完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王佩華(Pei-Hwa Wang) | |
| dc.contributor.author | Yu-Hsuan Wu | en |
| dc.contributor.author | 吳宇軒 | zh_TW |
| dc.date.accessioned | 2021-06-15T16:11:21Z | - |
| dc.date.available | 2020-08-28 | |
| dc.date.copyright | 2015-08-28 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-18 | |
| dc.identifier.citation | 王之仰、江友中、邱明堂、徐志宏、張誌益、莊國賓、傅龍明、陳又嘉、陳福旗、 陳幼光、廖明輝、劉宏仁、鄭雪玲。2011。分子檢測技術實習，第 87-110 頁。 國立屏東科技大學。屏東縣。 王少龍。2005。中國鯪鯉的野外生活習性初步觀察。引進與咨詢 4：52-53。 王珮蓉。2007。臺灣穿山甲救傷通報系統在保育上的應用。國立臺灣大學生態 學與演化生物學系碩士論文。臺北市。 史有青、汪運根。1985。穿山甲的食蟻習性。野生動物 6：42-43。 江海聲、馮敏、黃金昌。1988。穿山甲－活動習性的初步觀察。野生動物 4：11-13。 吳詩寶、馬廣智、唐玫、陳海、劉迺發。2002。中國穿山甲資源現狀及保護對 策。自然資源學報 17：174-180。 吳詩寶、劉廼發、李友余、孫儒泳。2005。中國穿山甲的食性與覓食行為初步觀 察。應用與環境生物學報 3：337-341。 吳詩寶、劉迺發、馬廣智、唐玫、陳海、徐昭榮。2004a。穿山甲生態學研究概 況。動物學雜誌 39：46-52。 吳詩寶、劉廼發、張迎梅、馬廣智。2004b。中國穿山甲受危狀況評估。應用與 環境生物學報 10：456-461。 吳詩寶、劉茜、馮干新、柯亞永。1999。中國穿山甲食物生熱營養素成分的初步 研究。湛江師范學院學報 (自然科學版) 20：74-76。 金仕謙、楊翕雯、莊薏瀚、連振曄、陳俊麟、王翰聰。2007。飼糧調整對臺灣穿 山甲（Manis pentadactyla pentadactyla）糞便菌相之影響。動物園學報 19： 51-59。 林敬勛。2010。臺東鸞山地區臺灣穿山甲（Manis pentadactyla pentadactyla）活 動範圍與洞穴利用之研究。國立屏東科技大學野生動物保育研究所碩士論 文。屏東縣。 房利祥、王少龍。1980。中國鯪鯉野外生活習性的初步觀察。北京自然博物館研 究報告 7：1-9。 范中衍。2005。 翡翠水庫臺灣穿山甲洞穴棲地研究。國立臺灣大學森林環境暨 資源學系碩士論文。臺北市。 柯亞永、常弘、吳詩寶、劉茜、馮干新。穿山甲主要食物營養成分研究。1999。 動物學研究 20：394-395。 張秀鑾。2007。基因多樣性分析。臺灣畜產種源資訊網。 http://www.angrin.tlri.gov.tw/seminar/20070821/35.pdf 張啟彥。2004。臺灣穿山甲飼糧表面消化率之研究。國立臺灣大學畜產學系碩士論文。臺北市。 野生動物保育法。2015。中華民國七十八年六月二十三日華總（一）義字第三二 六六號令制定公布。中華民國八十三年十月二十九日總統華總（一）義字第六 五二五號令修正公布。 詹芳澤、王齡敏。2010。野生臺灣穿山甲的救援。科學發展月刊 465：42-45。 詹雅婷。2009。圈養臺灣穿山甲繁殖行為與親子關係之研究。屏東科技大學野生 動物保育研究所碩士論文。屏東縣。 臺北野生動物貿易研究委員會。2010。國際野生物貿易研究組織。 http://www.wow.org.tw/traffic/tcm/index.files/Page554.htm 臺灣生物多樣性資源入口網。2015。中央研究院生物多樣性研究中心。 http://taibif.tw/zh/namecode/380589 趙榮台。1989。臺灣穿山甲（Manis pentadactyla pentadactyla）之繁殖保存研究： 一般生物學與現況分析。行政院農業委員會。臺北市。 趙榮台。1991。穿山甲的生物學及其保育。Proceeding of the first international symposium on wildlife conservation 319-331。 劉振河、徐龍輝。1981。穿山甲的生活習性與資源保護問題。動物學雜誌 16： 40-41。 顧文儀。1983。穿山甲飼養方法初探。動物學雜誌 3：26-28。 Akcakay, H., and P. Sjogren-Gulve. 2000. Population viability analyses in conservation planning: An overview. Ecol. Bull. 48: 9-21. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403-410. Arnason, E., and D. M. Rand. 1992. Heteroplasmy of short tandem repeats in Atlantic cod (Gadus morhua). Genetics 132: 211-220. Arnason, U., and E. Johnsson. 1992. The complete mitochondrial DNA sequence of the harbor seal, Phoca vitulina. J. Mol. Evo1. 34: 493-505. Attardi, G. 1985. Animal mitochondial DNA : an extreme example of genetic economy. Int. Rev. Cytol. 93: 93-145. Avise, J. C., S. Helfoan, C. Saunders, and L. S. Hales. 1986. Mitochondrial DNA differentiation in North Atlantic eels: population genetic consequences of an unusual life history pattern. Proc. Natl. Acad. Sci. (U. S. A.) 83: 4350-4354. Baker, A., and H. Marshall. 1997. Mitochondrial control region sequences as tools for understanding evolution. Page 51-382 in Mindell DP (ed) Avian molecular evolution and systematics. Academic Press, London, UK. Baker, C. S., A. Perry, G. K. Chambers, and P. J. Smith. 1995. Population variation in the mitochondrial cytochrome b gene of the orange roughy Holplosethus atlanticus and the hoki Macruronus novaezelandiae. Mar. Biol. 122: 503-509. Barbara, T., C. Palma-Silva, G. M. Paggi, F. Bered, and M. F. Fay. 2007. Cross-species transfer of nuclear microsatellite markers: Potential and limitations. Mol. Ecol. 16: 3759-3767. Barry, G. H. 2007. Phylogenetic trees made easy: a how-to manual. 3rd ed. Sinauer Assoc. Inc., Sunderland, MA, USA. Botstein, D., R. L. White, M. Skolnick, and R. W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32: 314-331. Brook, B., D. Tonkyn, J. O'Grady, and R. Frankham. 2002. Contribution of inbreeding to extinction risk in threatened species. Conserv. Ecol. 6: 16. [online] URL: http://www.consecol.org/vol6/iss1/art16. Brown, G. G., G. Gadaleta, G. Pepe, C. Saccone, and E. Sbisa. 1986. Structural conservation and variation in the D-loop-containing region of vertebrate mitochondrial DNA. J. Mol. Biol. 192: 503-511. Brown, W. M., M. George, Jr, and A. C. Wilson. 1979. Rapid evolution of animal mitochondrial DNA. Proc. Natl. Acad. Sci. (U. S. A.) 76: 1967-1971. Brown, W. M. 1985. The mitochondrial genome of animals. Page 95-130 in Molecular Evolutionary Genetics, ed. R. J. MacIntyre. Buroker, E., J. R. Brown, T. A. Gilbert, P. J. O’Hara, A. T. Beckenbach, W. K. Thomas, and M. J. Smith. 1990. Length heteroplasmy of sturgeon mitochondrial DNA: an illegitimate elongation model. Genetics 124: 157-163. Chapuis, M. P., and A. Estoup. 2007. Microsatellite null alleles and estimation of population differentiation. Mol. Biol. Evol. 24: 621-631. Clayton, D. A. 1982. Replication of animal mitochondrial DNA. Cell 28: 693-705. Coulson, I. 1989. The pangolin (Manis temmincki Smuts, 1932) in Zimbabwe. Afr. J. Ecol. 27: 149-155. DeNise, S., E. Johnston, J. Halverson, K. Marshall, D. Rosenfeld, S. McKenna, T. Sharp, and J. Edwards. 2002. Power of exclusion for parentage verification and probability of match for identity in American kennel club breeds using 17 canine microsatellite markers. Int. J. Legal Med. 116: 64-67. De Jong, M. A., N. Wahlberg, M. van Eijk, P. M. Brakefield, and B. J. Zwaan. 2011. Mitochondrial DNA signature for range-wide populations of Bicyclus anynana suggests a rapid expansion from recent refugia. PLoS ONE 6: e21385. Dieringer, D., and C. Schl ouml;tterer. 2003. MICROSATELLITE ANALYSER (MSA): a platform independent analysis tool for large microsatellite data sets. Mol. Ecol. Notes 3: 167-169. Dohm, J. C., C. Lottaz, T. Borodina, and H. Himmelbauer. 2008. Substantial biases in ultra-short read data sets from high-throughput DNA sequencing. Nucleic Acids Res. 36: e105. Douzery, E., and F. M. Catzeflis. 1995. Molecular evolution of the mitochondrial 12S ribosomal-RNA in Ungulata (Mammalia). J. of Mol. Evol. 41: 622-636. Efron, B., E. Halloran, and S. Holmes. 1996. Bootstrap confidence levels for phylogenetic trees. Proc. Natl. Acad. Sci. (U. S. A.) 93: 13429-13433. Ellegren, H. 2000. Heterogeneous mutation processes in human microsatellite DNA sequences. Nat. Genet. 24: 400-402. Ellegren, H. 2004. Microsatellites: Simple sequences with complex evolution. Nat. Rev. Genet. 5: 435-445. Evanno, G., S. Regnaut, and J. Goudet. 2005. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol. Ecol. 14: 2611-2620. Evett, I. W., and B. S. Weir. 1998. Interpreting DNA evidence: statistical genetics for forensic scientists.1st ed. Sinauer Assoc. Inc., Sunderland, MA, USA. Faircloth, B. C. 2008. MSATCOMMANDER: detection of microsatellite repeat and automated, locus-specific primer design. Mol. Ecol. Resour. 8: 92-94. Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783-791. Felsenstein, J. 2002. Phylogeny Inference Package (PHYLIP). Department of genomes scuences and department of Genetics, Univ. of Washington, Seattle, WA, USA. Fisher, R. A. 1953. Population genetics. Proc. Roy. Soc. B. 141: 510-523. Fonteque, G. V., J. Battilana, E. Paludo, and C. A. D. V. Lima-Rosa. 2014. Genetic polymorphism of fifteen microsatellite loci in Brazilian (blue-egg Caipira) chickens. Pesquisa Vet. Brasil. 34: 98-102. Gaubert, P., and A. Antunes. 2005. Assessing the taxonomic status of the Palawan pangolin Manis Culionensis (pholidota) using discrete morphological characters. J. Mammal. 86: 1068-1074. Gautschi, B., J. Muller, B. Schmid, and J. Shykoff. 2003. Effective number of breeders and maintenance of genetic diversity in the captive bearded vulture population. Heredity 91: 9-16. Gemayel, R., M. D. Vinces, M. Legendre, and K. J. Verstrepen. 2010. Variable tandem repeats accelerate evolution of coding and regulatory sequences. Annu. Rev. Genet. 44: 445-477. Ghivizzani, S. C., S. L. D. Mackay, C. S. Madse, P. J. Laipis, and W. W. Hauswirth. 1993. Transcribed heteroplasmic repeated sequences in the porcine mitochondrial DNA D-loop region. J. Mol. Evol. 37: 36-47. Gilpin, M. E., and M. E. Soul eacute;. 1986. Minimum viable populations: Processes of species extinction. Page 19-34 in: Soul eacute;, M. E. (ed.), Conservation biology: the science of scarcity and diversity. Sinauer, Sunderland, MA. Glenn, T. C., and N. A. Schable. 2005. Isolating microsatellite DNA loci. Meth. Enzymol. 395: 202-222. Goldstein, D., and C. Schl ouml;tterer. 1999. Functional roles of microsatellites and minisatellites. Page 10-23 in Microsatellites: Evolution and Applications. Oxford Univ. Press. Oxford. Guo, S. W., and E. A. Thompson. 1992. Performing the exact test of Hardy-Weinberg proportion for multiple alleles. Biometrics 48: 361-372. Guo, X., and R. Elston. 1999. Linkage information content of polymorphic genetic markers. Hum. Hered. 49: 112-118. Gray, W. M. 1989. Origin and evolution of mitochondrial DNA. Annu. Rev. Cell Biol. 5: 25-50. Green, M. R., and J. Sambrook. 2012. Preparation of plasmid DNA by Alkaline Lysis with SDS: Minipreps. Volume 1. Page 11-14 in molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA. Gyllensten, U., D. Wharton , A. Josefsson, and A. C. Wilson. 1991. Paternal inheritance of mitochondrial DNA in mice. Nature 352: 255-257. Hamilton, M. B., E. L. Pincus, A. Di Flore, and R. C. Fleischer. 1999. Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27: 500-507. Hayasaka, K., T. Ishida, and S. Horai. 1991. Heteroplasmy and polymorphism in Japanese monkeys: association with tandemly repeated sequences. Mol. Biol. Evol. 8: 399-415. Hoelzel, A. R., J. M. Hancock, and G. A. Dover. 1993. Generation of VNTRs and heteroplasmy by sequence turnover in the mitochondrial control region of two elephant seal species. J. Mol. Evol. 37: 190-197. Hoelzel, A. R., J. V. lopez, G. A. Dover, and S. J. O’brien. 1994. Rapid evolution of a heteroplasmic repetitive sequence in the mitochondrial DNA control region of carnivores. J. Mol. Evol. 39: 191-199. Holsinger, K. E. 2000. Demography and extinction in small population. Page 55-74 in Genetics, Demography, and Viability of Fragmented Populations. Cambridge Univ. Press, Cambridge. UK. Hoshino, A. A., J. P. Bravo, P. M. Nobile, and K. A. Morelli. 2012. Microsatellites as tools for genetic diversity analysis. Page 149-170 in Genetic Diversity in Microorganisms. M. Calisken, ed. InTech open access publisher, Rijeka, Croatia. Hsieh, H., J. C. Lee, J. Wu, C. Chen, Y. Chen, G. Wang, S. Chin, L. Wang, A. Linacre, and L. Tsai. 2011. Establishing the pangolin mitochondrial D-loop sequences from the confiscated scales. Forensic Sci. Int. Genet. 5: 303-307. Hu, N., O. Joseph, B. Huang, K. He, and X. Jiang. 2014. Isolation and characterization of thirteen microsatellite loci for the western black crested gibbon (Nomascus concolor) by high-throughput sequencing. Conserv. Genet. Resour. 6: 179-181. Hunter, R. L., and C. L. Markert. 1957. Histonchemical demonstration of enzymes separated by zone electrophoresis in starch gels. Science 125: 1294-1295. Jarne, P., and P. Lagoda. 1996. Microsatellites, from molecules to populations and back. Trends Ecol. Evol. 11: 424-429. Jamieson, A. 1965. The genetics of transferrin in cattle. Heredity 20: 419-441. Jamieson, A., and S. C. Taylor. 1997. Comparisons of three probability formulae for parentage exclusion. Anim. Genet. 28: 397-400. Johns, G. C., and J. C. Avise. 1998. A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene. Mol. Biol. Evol. 15: 1481-1490. Johnston, I. 2013. The plight of the pangolin: One of the planet's most extraordinary and intelligent animals is being hunted to extinction. The Independent. Retrieved from http://www.independent.co.uk Kandpal, R. P., G. Kandpal, and S. M. Weissman. 1994. Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-specific markers. Proc. Natl. Acad. Sci. (U. S. A.) 91: 88-92. Kang, S. 2008. Which exact test is more powerful in testing the Hardy–Weinberg law? Commun. Stat. Simul. Comput. 37: 14-24. Karagyozov, L., I. Kalcheva, and V. Chapman. 1993. Construction of random smallinsert genomic libraries highly enriched for simple sequence repeats. Nucleic Acids Res. 21: 3911-3912. Keller, L. F., and D. M. Waller. 2002. Inbreeding effects in wild populations. Trends Ecol. Evol. 17: 230-241. Kijas, J., J. Fowler, C. Garbett, and M. Thomas. 1994. Enrichment of microsatellites from the citrus genome using biotinylated oligonucleotide sequences bound to streptavidincoated magnetic particles. BioTechniques 16: 656-662. Kircher, M., and J. Kelso. 2010. High-throughput DNA sequencing - concepts and limitations. Bioessays 32: 524-536. Kim, J., E. Eizirik, S. J. O’Brien, and W. E. Johnson. 2001. Structure and patterns of sequence variation in the mitochondrial DNA control region of the great cats. Mitochondrion 14: 279-292. Kimura, M., and J. F. Crow. 1964. The number of alleles that can be maintained in a finite population. Genetics 49: 725-738. Koskinen, M. T., H. Hirvonen, P. A. Landry, and C. R. Primmer. 2004. The benefits of increasing the number of microsatellites utilized in genetic population studies: an empirical perspective. Hereditas 141: 61-67. Lachish, S., K. J. Miller, A. Storfer, A. W. Goldizen, and M. E. Jones. 2011. Evidence that disease-induced population decline changes genetic structure and alters dispersal patterns in the Tasmanian devil. Heredity 106: 172-182. Lande, R. C. 1993. Risks of population extinction from demographic and environmental stochasticity and random catastrophes. Am. Nat. 142: 911-927. Lande, R. C. 1998. Anthropogenic, ecological and genetic factors in extinction and conservation. Res. Popul. Ecol. 40: 259-269. Leberg, P. L. 2002. Estimating allelic richness: effects of sample size and bottlenecks. Mol. Ecol. 11: 2445-2449. Lin, L.Y., I. P. Chen, C. S. Tzeng, and P. C. Huang. 1991. Maternal transmission of mitochondrial DNA in ducks. Bioch. Biophy. Res. Comm. 168: 188-193. Liu, H., Y. Ran, H. Shi, X. Jia, and L. Zhao. 2015. Isolation and characterization of 18 microsatellite loci in the Chinese Noctule bat, Nyctalus plancyi. Conserv. Genet. Resour. 7: 295-297. Liu, Z. J., and J. F. Cordes. 2004. DNA marker technologies and their applications in aquaculture genetics. Aquaculture 238: 1-37. Lopez, J. V., N. Yuhki, R. Masuda, W. Modi, and S. J. O'Brien. 1994. Numt, a recent transfer and tandem amplification of mitochondrial DNA to the nuclear genome of the domestic cat. J. Mol. Evol. 39: 174-190. Luo, S. J., Q. X. Cai, V. A. David, L. Zhang, P. Martelli, N. T. L. Lim, N. Ferrand, S. C. Chin, P. Gaubert, M. J. Ramos, S. J. O’Brien, A. Antunes, and W. E. Johnson. 2007. Isolation and characterization of microsatellite markers in pangolins (Mammalia, Pholidota, Manis spp.). Mol. Ecol. Notes. 7: 269-272. Lynch, M., and B. G. Milligan. 1994. Analysis of population genetic structure with RAPD markers. Mol. Ecol. 3: 91-99. Mignotte, F. M., M. Gueride, A. M. Champagne, and J. C. Mounolou. 1990. Direct repeats in the non-coding region of rabbit mitochondrial DNA: involvement in the generation of intra and inter-individual heterogeneity. Eur. J. Biochem. 194: 561-571. Moritz, C., and W. M. Brown. 1987. Tandem duplications in animal mitochondrial DNAs : variation in incidence and gene content among lizards. Proc. Natl. Acad. Sci. (U. S. A.), 84: 7183-7187. Murphy, W. J., E. Eizirik, W. E. Johnson, Y. P. Zhang, O. A. Ryder, and S. J. O'Brien. 2001. Molecular phylogenetics and the origins of placental mammals. Nature 409: 614-618. Neff, B. D., and M. R. Gross. 2001. Microsatellite evolution in vertebrates: inference from AC dinucleotide repeats. Evolution 55: 1717-1733. Nei, M. 1972. Genetic distance between populations. Am. Nat. 106: 283-292. Nei, M. 1977. F-statistics and analysis of gene diversity in subdivided populations. Ann. Hum. Genet. 41: 225-233. Nei, M. 1987. Molecular evolutionary genetics. Columbia Univ. Press, NY, USA. Nisa, C., S. Agungpriyono1, N. Kitamura, M. Sasaki, J. Yamada, and K. Sigit1. 2010. Morphological features of the stomach of Malayan Pangolin. Anat. Histol. Embryol. 39: 432-439. Nowak, R. M. 1999. Walker’s mammals of the world, 6th ed., vol. 2. The John Hopkins University Press. Baltimore and London. Paetkau, D. 1999. Microsatellites obtained using strand extension: an enrichment protocol. Biotechniques 26: 690-697. Park, S. D. E. 2001. Trypanotolerance in West African cattle and the population genetic effects of selection. Ph. D. Thesis. Trinity College, Univ. of Dublin, Ireland. Pemberton, C. 2011. Distribution of the Pangolins derived from constituent species maps. Retrieved from: https://commons.wikimedia.org/wiki/File:Manis_ranges. png Primmer, C., J. Painter, M. Koskinen, J. Palo, and J. Merila. 2005. Factors affecting avian crossspecies microsatellite amplification. J. Avian Biol. 36: 348-360. Pritchard, J. K., M. Stephen, and P. Donnelly. 2000. Inference of population structure using multilocus genotype data. Genetics 155: 945-959. Qin, X. M., S. R. Dou, Q. X. Guan, P. S. Qin, and Y. She. 2012. Complete mitochondrial genome of the Manis pentadactyla (Pholidota, Manidae): Comparison of M. pentadactyla and M. tetradactyla. Mitochondr. DNA 23: 37-38. Quail, M. A., I. Kozarewa, F. Smith, A. Scally, P. J. Stephens, R. Durbin, H. Swerdlow, and D. J. Turner. 2008. A large genome center's improvements to the Illumina sequencing system. Nat. Methods 5: 1005-1010. Raimondi, V. B., G. Maruyama Mori, M. R. Piedrabuena, L. Wolfenson, and P. Mirol. 2012. Isolation and characterization of fifteen microsatellite loci from the endangered pampas deer (Ozotoceros bezoarticus, Cervidae). Conserv. Genet. Resour. 4: 1089-1092. Rao, M., S. Htun, T. Zaw, and T. Myint. 2010. Hunting, livelihoods and declining wildlife in the Hponkanrazi Wildlife Sanctuary, north Myanmar. Environ. Manage. 46: 143-153. Rassmann, K., C. Schl ouml;tterer, and D. Tautz. 1991. Isolation of simple-sequence loci for use in polymerase chain reaction-based DNA fingerprinting. Electrophoresis 12: 113-118. Richard, R. L., A. F. Richard, and M. A. Riley. 2003. Genetic population structure of the white sifaka (Propithecus verreauxi verreauxi) at Beza Mahafaly Special Reserve, southwest Madagascar (1992-2001). Mol. Ecol. 12: 2307-2317. Rohlfs, R. V., and B. S. Weir. 2008. Distributions of Hardy-Weinberg equilibrium test statistics. Genetics 180: 1609-1616. Rosenberg, N. A., T. Burke, K. Elo, M. W. Feldman, P. J. Freidlin, M. A. M. Groenen, J. Hillel, A. M auml;ki-Tanila, M. Tixier-Boichard, A. Vignal, K. Wimmers, and S. Weigend. 2001. Empirical evaluation of genetic clustering methods using multilocus genotypes from 20 chicken breeds. Genetics 159: 699-713. Rousset, F. 2007. GENEPOP ’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol. Ecol. Res. 8: 103-106. Rozen, S., and H. J. Skaletsky. 2000. Primer 3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 132: 365-386. Saitou, N., and M. Nei. 1987. The Neighbor-joining method: a method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425. Sang, T. K., H. Y. Chang, C. T. Chen, and C. F. Hui. 1994. Population structure of the Japanese eel, Anguilla japonica. Mol. Biol. Evol. 11: 250-260. Schlitter, D. A. 1993. Order Pholidota. Page 415 in Mammal species of the world: a taxonomic and geographic reference (D. E. Wilson and D. M. Reeder, eds.). 2nd ed. Smithsonian Institution Press, Washington, D. C.. Schl ouml;tterer, C. 2004. The evolution of molecular markers just a matter of fashion? Nat.Rev. Genet. 5: 63-69. Shadel, G. S., and D. A. Clayton. 1997. Mitochondrial DNA maintenance in vertebrates. Annu. Rev. Biochem. 66: 409-435. Shaffer, M. L. 1981. Minimum population sizes for species conservation. Bioscience 31: 131-134. Simon, C., F. Frati, A. Beckenbach, B. Crespi, H. Liu, and P. Flook. 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann. Entomol. Soc. Am. 87: 651-701. Slate, J., T. C. Marshall, and J. M. Pemberton. 2000. A retrospective assessment of the accuracy of the paternity inference program CERVUS. Mol. Ecol. 9: 801-808. Southern S., J. S. Peter, and E. D. Andrew. 1988. Molecular characterization of a cloned dolphin mitochondrial genome. J. Mol. Evol. 28: 32-42. Spielman, D., B. W. Brook, and R. Frankham. 2004. Most species are not driven to extinction before genetic factors impact them. Proc. Natl. Acad. Sci. (U. S. A.) 42: 15261-15264. Su, B., R. Q. Liu, Y. X. Wang, and L. M. Shi. 1994. Genetic diversity in the Chinese Pangolin (Manis pentadactyla) inferred from protein electrophoresis. Biochem. Genet. 32: 343-349. Sutovsky, P., R. D. Moreno, J. RamalhoSantos, T. Dominko, C. Simerly, and G. Schatten. 1999. Development: Ubiquitin tag for sperm mitochondria. Nature 402: 371-372. Tamaki, K. 2007. Molecular forensics. Page 71-86 in Minisatellite and microsatellite DNA typing analysis. John Wiley and Sons, Ltd. England. Tamura K. D., Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28: 2731-2739. Tateno, Y., N. Takezaki, and M. Nei. 1994. Relative efficiencies of the maximum-likelihood, neighbor-joining, and maximum-parsimony methods when substitution rate varies with site. Mol. Biol. Evol. 11: 261-277. Tautz, D. 1989. Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucleic Acids Res. 17: 6463-6471. Templeton, A. R., K. A. Crandall, and C. F. Sing. 1992. A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation. Genetics 132: 619-633. Toth, G., Z. Gaspari, and J. Jurka. 2000. Microsatellites in different eukaryotic genomes: Survey and analysis. Genome Res. 10: 967-981. Vecchia, A. C. D., C. Biondo, A. Sanches, A. Keuroghlian, C. Y. Miyaki, M. Galetti, and P. M. Galetti. 2011. Isolation and characterization of microsatellite loci for white-lipped peccaries (Tayassu pecari) and cross-amplification in collared peccaries (Pecari tajacu). Conserv. Genet. Resour. 3: 151-154. Van Peenan, P. F. D., R. H. Light, and J. F. Duncan. 1969. Observations on mammals of MT. Sontra, south Vietnan. Mammalia 35: 126-143. Vignal, A., D. Milan, M. SanCristobal, and A. Eggen. 2002. A review on SNP and other types of molecular markers and their use in animal genetics. Genet. Sel. Evol. 34: 275-305. Waits, L. P., G. Luikart, and P. Taberlet. 2001. Estimating the probability of identity among genotypes in natural populations: cautions and guidelines. Mol. Ecol. 10: 249-256. Wang, R., L. Zheng, Y. T. Toure, T. Dandekar, and F. C. Kafatos. 2001. When genetic distance matters: measuring genetic differentiation at microsatellite loci in whole-genome scans of recent and incipient mosquito species. Proc. Natl. Acad. Sci. (U. S. A.) 98: 10769-10774. Wang, F., Z. X. Peng, J. G. Zhang, L. Tian, H. B. Han, Z. C. Cao, Z. H. Zhang, F. J. Shen, D. S. Li, H. M. Zhang, L. B. Zhang, and Z. X. Lian. 2007. Studies on genetic diversity of captive giant pandas (Ailuropoda melanoleuca) based on microsatellite DNA markers. Prog. Biochemi. Biophys. 34: 1279-1287. Wang, H., X. Luo, W. B. Shi, and B. W. Zhang. 2014. Isolation and characterization of polymorphic microsatellite loci of the Chinese muntjac (Muntiacus reevesi). Genet. Mol. Res. 13: 1905-1908. Waterman, M. S., M. Eggert, and E. Lander. 1992. Parametric sequence comparisons. Proc. Natl. Acad. Sci. (U. S. A.) 89: 6090-6093. Weber, J. 1990. Informativeness of human (dCdA)n•(dGdT)n polymorphisms. Genomics 7: 524-530. Wigginton, J. E., D. J. Cutler, and G. R. Abecasis. 2005. A note on exact tests of Hardy-Weinberg equilibrium. Am. J. Hum. Genet. 76: 887-893. Wilkinson, G. S., and A. M. Chapman. 1991. Length and sequence variation in evening bat D-loop mtDNA. Genetics 128: 607-617. Wright, S. 1951. The genetical structure of populations. Ann. Eugen. 15: 323-354. Wright, S. 1965. The interpretation of population structure by F-Statistics with special regard to systems of mating. Evolution 19: 395-420. Wright, S. 1978. Evolution and Genetics of Populations. Vol. 4 Variability Within and Among Natural Populations. Univ. of Chicago Press, Chicago, USA. Zane, L., L. Bargelloni, and T. Patarnello. 2002. Strategies for microsatellite isolation: A review. Mol. Ecol. 11: 1-16. Zhan, A., Z. Bao, X. Hu, W. Lu, and J. Hu. 2008. Methods comparison for microsatellite marker development: Different isolation methods, different yield efficiency. J. Ocean Univ. China 8: 161-165. Zhang, Y. P., and L. M. Shi. 1991. Genetic diversity in the Chinese Pangolin (Manis Pentadactyla): Inferred from restriction enzyme analysis of mitochondrial DNAs. Biochem. Genet. 29: 9-10. Zhou, K., A. Aertsen, and C. W. Michiels. 2014. The role of variable DNA tandem repeats in bacterial adaptation. FEMS Microbiol. Rev. 38: 119-141. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52300 | - |
| dc.description.abstract | 臺灣穿山甲（Formosan pangolin, Manis pentadactyla pentadactyla ）屬於臺灣特有種，在國內屬於珍貴稀有保育動物，利用分子遺傳標識了解族群的遺傳變異性（genetic variability）與遺傳結構（genetic structure），有助於進行族群遺傳管理與遺傳保育。而目前分子標識中以微衛星標識較為廣泛應用，因此本試驗之目的為開發適用於臺灣穿山甲族群之微衛星標識，以作為遺傳監測的有力工具。另外，粒線體 DNA 控制區域也同樣被選為分子遺傳標識對臺灣穿山甲進行分析，期望結合核內與核外的遺傳資訊，得到更完整的臺灣穿山甲族群遺傳結構。 本試驗挑選 18 組自馬來西亞穿山甲（Manis javanica ）所開發的微衛星基因座，作為跨物種微衛星標識進行研究，結果顯示，僅有 5 組微衛星基因座可在臺灣穿山甲 DNA 成功增幅並具有高多態性，與應用於原物種的結果比較，交替基因數（number of alleles, Na）、平均觀測異質度（observed heterozygosity, HO）與平均期望異質度（expected heterozygosity, HE）皆有所下降。因此，本試驗遂以選擇性雜合法（selective hybridization），將含有重複序列片段（repeat units）之探針（probe）與一公一母之臺灣穿山甲基因體 DNA進行雜合，建構微衛星序列豐富基因庫，從中挑選出 10 組新的微衛星基因座。結合此 10 組新微衛星標識與 5 組跨物種微衛星標識，檢測 36 隻臺灣穿山甲之遺傳變異，結果顯示此 15 組微衛星標識的交替基因數（Na）與有效交替基因數（number of effective alleles, Ne）之平均值各為 4.9±2.2 與 2.8±1.5；觀測異質度（HO）與期望異質度（HE）之平均值分別為 0.585±0.170 及 0.600±0.142；多態性訊息含量（polymorphic information content, PIC）之平均值為 0.538±0.154。FIS的平均值為 0.011±0.244，代表此 36 隻臺灣穿山甲的近親程度不嚴重。結合 15 組微衛星基因座之綜合個體鑑別率（probability of identity, P(ID)）與綜合近親個體鑑別率（probability of identity among sibs, P(ID)sib）分別為 2.916×10-11 與 3.261×10-5；另外，此 15 組新微衛星標識之親子排除率（power of exclusion, PE）為 98.02%。以 15 組微衛星標識分析臺灣穿山甲族群遺傳結構結果顯示，南投地區的穿山甲可以被區分成不同的群集。 在臺灣穿山甲粒線體 DNA的研究方面，本試驗成功增幅的控制區域序列長度為 915 bp，序列分析結果顯示，在 36 隻臺灣穿山甲樣本中，共可發現 5 種單套型（haplotype），單套型多樣性指數（haplotype diversity, h）及核苷酸多樣性指數（nucleotide diversity, π）在中部地區穿山甲最高（0.810與0.00189）。以單套型所繪製的最小關聯網狀圖（minimum spanning network）結果顯示，單套型E僅出現於南投地區的穿山甲。 綜合所述，本研究開發出 15 組微衛星基因座，不僅可作為遺傳監控的重要分子遺傳標識，亦可用於身分辨識與推測系譜。此外，結合微衛星標識與粒線體控制區域的多態性分析結果顯示，南投地區的臺灣穿山甲與其他地區有較明顯的分化情形，可視為獨立的保育管理單位，而整體穿山甲族群之近親程度不嚴重，也沒有遺傳歧異度喪失的情形，但仍應持續進行遺傳監測以進行遺傳保育。 | zh_TW |
| dc.description.abstract | Formosan pangolin（Manis pentadactyla pentadactyla ）is a rare species which is endemic to Taiwan. To manage and conserve this species, information of the genetic variability and genetic structure is important. In molecular genetic markers, microsatellite marker seems to be the most sultable markers for this purpose. Therefore, the objective of this study was to develop suitable microsatellite markers for Formosan pangolins to process genetic monitoring. On the other hand, the mitochondrial DNA control region was also used as molecular genetic marker. Through combining informations of genetic diversity obtained from nuclear DNA and mitochondrial DNA, the more complete genetic structure of Formosan pangolins would be revealed. Corss-species microsatellite markers isolated from Manis javanica were used in this study. The results showed that there were only 5 sets of microsatellite loci could amplified successfully and displayed high polymorphisms in a total of 18 loci in Formosan pangolin DNA. The number of alleles（Na）, observed heterozygosity（HO）, and expected heterozygosity（HE）were lower when compared with the markers used in Manis javanica. Therefore, we isolated 10 sets of novel microsatellite markers from one male and one female Formosan pangolins DNA using a selective hybridization method with mixed probes containing different repeat units from a microsatellite- enriched library. The 10 sets of novel microsatellite markers and 5 sets of cross-species microsatellite markers were combined to analyze the genetic variability of 36 Formosan pangolins. The results showed that the average number of alleles（Na） and the average number of effective alleles（Ne）were 4.9±2.2 and 2.8±1.5, respectively. The average observed heterozygosity（HO）and average expected heterozygosity（HE）were 0.585±0.170 and 0.600±0.142, respectively. The average polymorphic information content（PIC）was 0.538±0.154. The average FIS was 0.011±0.244, indicating the low inbreeding level. The total propability of identity（P(ID)）and total probability of identity among sibs（P(ID)sib）were 2.916×10-11 and 3.261×10-5, respectively. Furthermore, the power of exclusion（PE）of the 15 sets of microsatellite markers was 98.02%. The results of using these 15 sets of microsatellite markers to analyze genetic structure of Formosan pangolins indicated that the individuals originated from Nantou could be separated from others. On the aspect of mitochondrial DNA studies, we amplified 915 bp control region sequence. We observed 5 haplotypes in 36 Formosan pangolin samples. The haplotype diversity（h）and nucleotide diversity（π）of Formosan pangolins were highest in central region（0.810 and 0.00189, respectively）. The results of minimum spanning network showed that haplotype E was only existed in pangolins originated from Nantou region. In conclusion, we developed 15 sets of microsatellite markers for Formosan pangolins. these tools were not only useful for genetic monitoring but also suitable for identifying individuals or inferring the parentage coordinating. Moreover, the microsatellite markers and mitochondrial DNA data showed that Formosan pangolins in Nantou region could be considered as a independent management unit of conservation. The inbreeding level was low in total Formosan pangolin population and it displayed sufficient genetic diversity. But the genetic monitoring should be carried on. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T16:11:21Z (GMT). No. of bitstreams: 1 ntu-104-R02626023-1.pdf: 2273319 bytes, checksum: d20f0d8a7e2be56573c7433dd9b17c1a (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 目錄 I 圖次 III 表次 IV 附表次 VI 中文摘要 1 英文摘要 3 文獻檢討 5 一、穿山甲之生物學研究 5 二、遺傳保育 10 三、研究目的 42 材料與方法 43 試驗一、臺灣穿山甲微衛星標識之開發與應用 43 一、跨物種微衛星標識 43 二、新微衛星標識之開發 46 三、遺傳關係分析 51 試驗二、臺灣穿山甲粒線體 DNA 控制區域序列分析 56 一、試驗動物 56 二、粒線體 DNA 控制區域序列定序 56 三、粒線體 DNA 控制區域序列分析 56 結果 59 試驗一、臺灣穿山甲微衛星標識之開發與應用 59 一、跨物種微衛星基因座與遺傳變異性 59 二、臺灣穿山甲新微衛星標識 59 三、臺灣穿山甲個體遺傳關係分析 67 試驗二、臺灣穿山甲粒線體 DNA 控制區域序列分析 76 一、控制區域序列分析 76 二、遺傳關係分析 76 討論 81 一、跨物種微衛星標識 81 二、臺灣穿山甲新微衛星標識 83 三、臺灣穿山甲粒線體 DNA 控制區歧異度 89 四、本試驗臺灣穿山甲族群遺傳結構 91 五、臺灣穿山甲個體鑑別與親子鑑別之應用 92 六、臺灣穿山甲管理策略 94 結論 95 參考文獻 96 附錄 112 | |
| dc.language.iso | zh-TW | |
| dc.subject | 微衛星標識 | zh_TW |
| dc.subject | 粒線體 DNA | zh_TW |
| dc.subject | 遺傳保育 | zh_TW |
| dc.subject | 穿山甲 | zh_TW |
| dc.subject | mitochondrial DNA | en |
| dc.subject | genetic conservation | en |
| dc.subject | Formosan pangolin | en |
| dc.subject | microsatellite marker | en |
| dc.title | 利用微衛星標識與粒線體DNA探討臺灣地區穿山甲之遺傳結構與多態性 | zh_TW |
| dc.title | Genetic Diversity and Genetic Structure of Formosan Pangolins in Taiwan Based on Microsatellite Markers and Mitochondrial DNA | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 林恩仲(En-Chung Lin) | |
| dc.contributor.oralexamcommittee | 蕭博文,曹先紹 | |
| dc.subject.keyword | 穿山甲,微衛星標識,粒線體 DNA,遺傳保育, | zh_TW |
| dc.subject.keyword | Formosan pangolin,microsatellite marker,mitochondrial DNA,genetic conservation, | en |
| dc.relation.page | 120 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-08-18 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 動物科學技術學研究所 | zh_TW |
| 顯示於系所單位： | 動物科學技術學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-104-1.pdf 未授權公開取用 | 2.22 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。