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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林乃君(Nai-Chun Lin) | |
| dc.contributor.author | Yew-Yee Lu | en |
| dc.contributor.author | 盧友義 | zh_TW |
| dc.date.accessioned | 2021-06-15T16:09:08Z | - |
| dc.date.available | 2020-08-28 | |
| dc.date.copyright | 2015-08-28 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-19 | |
| dc.identifier.citation | 1. Gardan L, Shafik H, Belouin S, Broch R, Grimont F, Grimont PA. 1999. DNA relatedness among the pathovars of Pseudomonas syringae and description of Pseudomonas tremae sp. nov. and Pseudomonas cannabina sp. nov. (ex Sutic and Dowson 1959). Int J Syst Bacteriol 49 Pt 2:469-478. 2. Cohn JR, Martin GB. 2005. Pseudomonas syringae pv. tomato type III effectors AvrPto and AvrPtoB promote ethylene-dependent cell death in tomato. Plant J 44:139-154. 3. Cuppels DA. 1986. Generation and Characterization of Tn5 Insertion Mutations in Pseudomonas syringae pv. tomato. Appl Environ Microbiol 51:323-327. 4. Whalen MC, Innes RW, Bent AF, Staskawicz BJ. 1991. Identification of Pseudomonas syringae pathogens of Arabidopsis and a bacterial locus determining avirulence on both Arabidopsis and soybean. Plant Cell 3:49-59. 5. Bronstein PA, Marrichi M, Cartinhour S, Schneider DJ, DeLisa MP. 2005. Identification of a twin-arginine translocation system in Pseudomonas syringae pv. tomato DC3000 and its contribution to pathogenicity and fitness. J Bacteriol 187:8450-8461. 6. Xin XF, He SY. 2013. Pseudomonas syringae pv. tomato DC3000: a model pathogen for probing disease susceptibility and hormone signaling in plants. Annu Rev Phytopathol 51:473-498. 7. Boureau T, Routtu J, Roine E, Taira S, Romantschuk M. 2002. Localization of hrpA-induced Pseudomonas syringae pv. tomato DC3000 in infected tomato leaves. Mol Plant Pathol 3:451-460. 8. Liu B, Hong YB, Zhang YF, Li XH, Huang L, Zhang HJ, Li DY, Song FM. 2014. Tomato WRKY transcriptional factor SlDRW1 is required for disease resistance against Botrytis cinerea and tolerance to oxidative stress. Plant Sci 227:145-156. 9. Liu B, Ouyang Z, Zhang Y, Li X, Hong Y, Huang L, Liu S, Zhang H, Li D, Song F. 2014. Tomato NAC transcription factor SlSRN1 positively regulates defense response against biotic stress but negatively regulates abiotic stress response. PLoS One 9:e102067. 10. Postel S, Kemmerling B. 2009. Plant systems for recognition of pathogen-associated molecular patterns. Semin Cell Dev Biol 20:1025-1031. 11. Ekengren SK, Liu Y, Schiff M, Dinesh-Kumar SP, Martin GB. 2003. Two MAPK cascades, NPR1, and TGA transcription factors play a role in Pto-mediated disease resistance in tomato. Plant J 36:905-917. 12. Bestwick CS, Bennett MH, Mansfield JW. 1995. Hrp Mutant of Pseudomonas syringae pv phaseolicola Induces Cell Wall Alterations but Not Membrane Damage Leading to the Hypersensitive Reaction in Lettuce. Plant Physiol 108:503-516. 13. Nishimura MT, Stein M, Hou BH, Vogel JP, Edwards H, Somerville SC. 2003. Loss of a callose synthase results in salicylic acid-dependent disease resistance. Science 301:969-972. 14. Gomez-Gomez L, Felix G, Boller T. 1999. A single locus determines sensitivity to bacterial flagellin in Arabidopsis thaliana. Plant J 18:277-284. 15. Melotto M, Underwood W, Koczan J, Nomura K, He SY. 2006. Plant stomata function in innate immunity against bacterial invasion. Cell 126:969-980. 16. Ferreira AO, Myers CR, Gordon JS, Martin GB, Vencato M, Collmer A, Wehling MD, Alfano JR, Moreno-Hagelsieb G, Lamboy WF, DeClerck G, Schneider DJ, Cartinhour SW. 2006. Whole-genome expression profiling defines the HrpL regulon of Pseudomonas syringae pv. tomato DC3000, allows de novo reconstruction of the Hrp cis clement, and identifies novel coregulated genes. Mol Plant Microbe Interact 19:1167-1179. 17. Schechter LM, Vencato M, Jordan KL, Schneider SE, Schneider DJ, Collmer A. 2006. Multiple approaches to a complete inventory of Pseudomonas syringae pv. tomato DC3000 type III secretion system effector proteins. Mol Plant Microbe Interact 19:1180-1192. 18. Vencato M, Tian F, Alfano JR, Buell CR, Cartinhour S, DeClerck GA, Guttman DS, Stavrinides J, Joardar V, Lindeberg M, Bronstein PA, Mansfield JW, Myers CR, Collmer A, Schneider DJ. 2006. Bioinformatics-enabled identification of the HrpL regulon and type III secretion system effector proteins of Pseudomonas syringae pv. phaseolicola 1448A. Mol Plant Microbe Interact 19:1193-1206. 19. Alfano JR, Charkowski AO, Deng WL, Badel JL, Petnicki-Ocwieja T, van Dijk K, Collmer A. 2000. The Pseudomonas syringae Hrp pathogenicity island has a tripartite mosaic structure composed of a cluster of type III secretion genes bounded by exchangeable effector and conserved effector loci that contribute to parasitic fitness and pathogenicity in plants. Proc Natl Acad Sci U S A 97:4856-4861. 20. Angot A, Peeters N, Lechner E, Vailleau F, Baud C, Gentzbittel L, Sartorel E, Genschik P, Boucher C, Genin S. 2006. Ralstonia solanacearum requires F-box-like domain-containing type III effectors to promote disease on several host plants. Proc Natl Acad Sci U S A 103:14620-14625. 21. Jiang BL, He YQ, Cen WJ, Wei HY, Jiang GF, Jiang W, Hang XH, Feng JX, Lu GT, Tang DJ, Tang JL. 2008. The type III secretion effector XopXccN of Xanthomonas campestris pv. campestris is required for full virulence. Res Microbiol 159:216-220. 22. Rojas CM, Ham JH, Schechter LM, Kim JF, Beer SV, Collmer A. 2004. The Erwinia chrysanthemi EC16 hrp/hrc gene cluster encodes an active Hrp type III secretion system that is flanked by virulence genes functionally unrelated to the Hrp system. Mol Plant Microbe Interact 17:644-653. 23. Alfano JR, Collmer A. 2004. Type III secretion system effector proteins: double agents in bacterial disease and plant defense. Annu Rev Phytopathol 42:385-414. 24. Anderson DM, Fouts DE, Collmer A, Schneewind O. 1999. Reciprocal secretion of proteins by the bacterial type III machines of plant and animal pathogens suggests universal recognition of mRNA targeting signals. Proc Natl Acad Sci U S A 96:12839-12843. 25. Galan JE, Collmer A. 1999. Type III secretion machines: bacterial devices for protein delivery into host cells. Science 284:1322-1328. 26. Hirano SS, Charkowski AO, Collmer A, Willis DK, Upper CD. 1999. Role of the Hrp type III protein secretion system in growth of Pseudomonas syringae pv. syringae B728a on host plants in the field. Proc Natl Acad Sci U S A 96:9851-9856. 27. Rahme LG, Mindrinos MN, Panopoulos NJ. 1992. Plant and environmental sensory signals control the expression of hrp genes in Pseudomonas syringae pv. phaseolicola. J Bacteriol 174:3499-3507. 28. Xiao Y, Lu Y, Heu S, Hutcheson SW. 1992. Organization and environmental regulation of the Pseudomonas syringae pv. syringae 61 hrp cluster. J Bacteriol 174:1734-1741. 29. Arlat M, Gough CL, Zischek C, Barberis PA, Trigalet A, Boucher CA. 1992. Transcriptional organization and expression of the large hrp gene cluster of Pseudomonas solanacearum. Mol Plant Microbe Interact 5:187-193. 30. Huynh TV, Dahlbeck D, Staskawicz BJ. 1989. Bacterial blight of soybean: regulation of a pathogen gene determining host cultivar specificity. Science 245:1374-1377. 31. Schulte R, Bonas U. 1992. A Xanthomonas Pathogenicity Locus Is Induced by Sucrose and Sulfur-Containing Amino Acids. Plant Cell 4:79-86. 32. Stauber JL, Loginicheva E, Schechter LM. 2012. Carbon source and cell density-dependent regulation of type III secretion system gene expression in Pseudomonas syringae pathovar tomato DC3000. Res Microbiol 163:531-539. 33. Wei ZM, Sneath BJ, Beer SV. 1992. Expression of Erwinia amylovora hrp genes in response to environmental stimuli. J Bacteriol 174:1875-1882. 34. Zuluaga AP, Puigvert M, Valls M. 2013. Novel plant inputs influencing Ralstonia solanacearum during infection. Front Microbiol 4:349. 35. Frederick RD, Ahmad M, Majerczak DR, Arroyo-Rodriguez AS, Manulis S, Coplin DL. 2001. Genetic organization of the Pantoea stewartii subsp. stewartii hrp gene cluster and sequence analysis of the hrpA, hrpC, hrpN, and wtsE operons. Mol Plant Microbe Interact 14:1213-1222. 36. Wei ZM, Beer SV. 1995. hrpL activates Erwinia amylovora hrp gene transcription and is a member of the ECF subfamily of sigma factors. J Bacteriol 177:6201-6210. 37. Xiao Y, Heu S, Yi J, Lu Y, Hutcheson SW. 1994. Identification of a putative alternate sigma factor and characterization of a multicomponent regulatory cascade controlling the expression of Pseudomonas syringae pv. syringae Pss61 hrp and hrmA genes. J Bacteriol 176:1025-1036. 38. Xiao Y, Hutcheson SW. 1994. A single promoter sequence recognized by a newly identified alternate sigma factor directs expression of pathogenicity and host range determinants in Pseudomonas syringae. J Bacteriol 176:3089-3091. 39. Genin S, Gough CL, Zischek C, Boucher CA. 1992. Evidence that the hrpB gene encodes a positive regulator of pathogenicity genes from Pseudomonas solanacearum. Mol Microbiol 6:3065-3076. 40. Wengelnik K, Bonas U. 1996. HrpXv, an AraC-type regulator, activates expression of five of the six loci in the hrp cluster of Xanthomonas campestris pv. vesicatoria. J Bacteriol 178:3462-3469. 41. Fouts DE, Abramovitch RB, Alfano JR, Baldo AM, Buell CR, Cartinhour S, Chatterjee AK, D'Ascenzo M, Gwinn ML, Lazarowitz SG, Lin NC, Martin GB, Rehm AH, Schneider DJ, van Dijk K, Tang X, Collmer A. 2002. Genomewide identification of Pseudomonas syringae pv. tomato DC3000 promoters controlled by the HrpL alternative sigma factor. Proc Natl Acad Sci U S A 99:2275-2280. 42. Innes RW, Bent AF, Kunkel BN, Bisgrove SR, Staskawicz BJ. 1993. Molecular analysis of avirulence gene avrRpt2 and identification of a putative regulatory sequence common to all known Pseudomonas syringae avirulence genes. J Bacteriol 175:4859-4869. 43. Shen H, Keen NT. 1993. Characterization of the promoter of avirulence gene D from Pseudomonas syringae pv. tomato. J Bacteriol 175:5916-5924. 44. Hendrickson EL, Guevera P, Ausubel FM. 2000. The alternative sigma factor RpoN is required for hrp activity in Pseudomonas syringae pv. maculicola and acts at the level of hrpL transcription. J Bacteriol 182:3508-3516. 45. Grimm C, Aufsatz W, Panopoulos NJ. 1995. The hrpRS locus of Pseudomonas syringae pv. phaseolicola constitutes a complex regulatory unit. Mol Microbiol 15:155-165. 46. Hutcheson SW, Bretz J, Sussan T, Jin S, Pak K. 2001. Enhancer-binding proteins HrpR and HrpS interact to regulate hrp-encoded type III protein secretion in Pseudomonas syringae strains. J Bacteriol 183:5589-5598. 47. Lin NC, Martin GB. 2005. An avrPto/avrPtoB mutant of Pseudomonas syringae pv. tomato DC3000 does not elicit Pto-mediated resistance and is less virulent on tomato. Mol Plant Microbe Interact 18:43-51. 48. Gohre V, Spallek T, Haweker H, Mersmann S, Mentzel T, Boller T, de Torres M, Mansfield JW, Robatzek S. 2008. Plant pattern-recognition receptor FLS2 is directed for degradation by the bacterial ubiquitin ligase AvrPtoB. Curr Biol 18:1824-1832. 49. Scofield SR, Tobias CM, Rathjen JP, Chang JH, Lavelle DT, Michelmore RW, Staskawicz BJ. 1996. Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of Tomato. Science 274:2063-2065. 50. Tang X, Frederick RD, Zhou J, Halterman DA, Jia Y, Martin GB. 1996. Initiation of Plant Disease Resistance by Physical Interaction of AvrPto and Pto Kinase. Science 274:2060-2063. 51. Tseng TT, Tyler BM, Setubal JC. 2009. Protein secretion systems in bacterial-host associations, and their description in the Gene Ontology. Bmc Microbiology 9. 52. Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordonez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ. 2006. A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science 312:1526-1530. 53. Pukatzki S, Ma AT, Sturtevant D, Krastins B, Sarracino D, Nelson WC, Heidelberg JF, Mekalanos JJ. 2006. Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system. Proc Natl Acad Sci U S A 103:1528-1533. 54. Ma J, Bao Y, Sun M, Dong W, Pan Z, Zhang W, Lu C, Yao H. 2014. Two functional type VI secretion systems in avian pathogenic Escherichia coli are involved in different pathogenic pathways. Infect Immun 82:3867-3879. 55. Ma LS, Hachani A, Lin JS, Filloux A, Lai EM. 2014. Agrobacterium tumefaciens deploys a superfamily of type VI secretion DNase effectors as weapons for interbacterial competition in planta. Cell Host Microbe 16:94-104. 56. Boyer F, Fichant G, Berthod J, Vandenbrouck Y, Attree I. 2009. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics 10:104. 57. Pukatzki S, Ma AT, Revel AT, Sturtevant D, Mekalanos JJ. 2007. Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin. Proc Natl Acad Sci U S A 104:15508-15513. 58. Schlieker C, Zentgraf H, Dersch P, Mogk A. 2005. ClpV, a unique Hsp100/Clp member of pathogenic proteobacteria. Biol Chem 386:1115-1127. 59. Bonemann G, Pietrosiuk A, Diemand A, Zentgraf H, Mogk A. 2009. Remodelling of VipA/VipB tubules by ClpV-mediated threading is crucial for type VI protein secretion. EMBO J 28:315-325. 60. Jobichen C, Chakraborty S, Li M, Zheng J, Joseph L, Mok YK, Leung KY, Sivaraman J. 2010. Structural basis for the secretion of EvpC: a key type VI secretion system protein from Edwardsiella tarda. PLoS One 5:e12910. 61. Basler M, Pilhofer M, Henderson GP, Jensen GJ, Mekalanos JJ. 2012. Type VI secretion requires a dynamic contractile phage tail-like structure. Nature 483:182-186. 62. Bonemann G, Pietrosiuk A, Mogk A. 2010. Tubules and donuts: a type VI secretion story. Mol Microbiol 76:815-821. 63. Kapitein N, Bonemann G, Pietrosiuk A, Seyffer F, Hausser I, Locker JK, Mogk A. 2013. ClpV recycles VipA/VipB tubules and prevents non-productive tubule formation to ensure efficient type VI protein secretion. Mol Microbiol 87:1013-1028. 64. Leiman PG, Basler M, Ramagopal UA, Bonanno JB, Sauder JM, Pukatzki S, Burley SK, Almo SC, Mekalanos JJ. 2009. Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin. Proc Natl Acad Sci U S A 106:4154-4159. 65. Pell LG, Kanelis V, Donaldson LW, Howell PL, Davidson AR. 2009. The phage lambda major tail protein structure reveals a common evolution for long-tailed phages and the type VI bacterial secretion system. Proc Natl Acad Sci U S A 106:4160-4165. 66. Russell AB, Peterson SB, Mougous JD. 2014. Type VI secretion system effectors: poisons with a purpose. Nat Rev Microbiol 12:137-148. 67. Russell AB, Hood RD, Bui NK, LeRoux M, Vollmer W, Mougous JD. 2011. Type VI secretion delivers bacteriolytic effectors to target cells. Nature 475:343-347. 68. Russell AB, Singh P, Brittnacher M, Bui NK, Hood RD, Carl MA, Agnello DM, Schwarz S, Goodlett DR, Vollmer W, Mougous JD. 2012. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe 11:538-549. 69. Whitney JC, Chou S, Russell AB, Biboy J, Gardiner TE, Ferrin MA, Brittnacher M, Vollmer W, Mougous JD. 2013. Identification, structure, and function of a novel type VI secretion peptidoglycan glycoside hydrolase effector-immunity pair. J Biol Chem 288:26616-26624. 70. Brooks TM, Unterweger D, Bachmann V, Kostiuk B, Pukatzki S. 2013. Lytic activity of the Vibrio cholerae type VI secretion toxin VgrG-3 is inhibited by the antitoxin TsaB. J Biol Chem 288:7618-7625. 71. English G, Trunk K, Rao VA, Srikannathasan V, Hunter WN, Coulthurst SJ. 2012. New secreted toxins and immunity proteins encoded within the Type VI secretion system gene cluster of Serratia marcescens. Mol Microbiol 86:921-936. 72. Russell AB, LeRoux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD. 2013. Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature 496:508-512. 73. Goeders N, Van Melderen L. 2014. Toxin-antitoxin systems as multilevel interaction systems. Toxins (Basel) 6:304-324. 74. Hood RD, Singh P, Hsu F, Guvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD. 2010. A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Cell Host Microbe 7:25-37. 75. Li M, Le Trong I, Carl MA, Larson ET, Chou S, De Leon JA, Dove SL, Stenkamp RE, Mougous JD. 2012. Structural basis for type VI secretion effector recognition by a cognate immunity protein. PLoS Pathog 8:e1002613. 76. Bingle LE, Bailey CM, Pallen MJ. 2008. Type VI secretion: a beginner's guide. Curr Opin Microbiol 11:3-8. 77. Sarris PF, Skandalis N, Kokkinidis M, Panopoulos NJ. 2010. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol 11:795-804. 78. Haapalainen M, Mosorin H, Dorati F, Wu RF, Roine E, Taira S, Nissinen R, Mattinen L, Jackson R, Pirhonen M, Lin NC. 2012. Hcp2, a secreted protein of the phytopathogen Pseudomonas syringae pv. tomato DC3000, is required for fitness for competition against bacteria and yeasts. J Bacteriol 194:4810-4822. 79. Bernard CS, Brunet YR, Gueguen E, Cascales E. 2010. Nooks and crannies in type VI secretion regulation. J Bacteriol 192:3850-3860. 80. Rao PS, Yamada Y, Tan YP, Leung KY. 2004. Use of proteomics to identify novel virulence determinants that are required for Edwardsiella tarda pathogenesis. Mol Microbiol 53:573-586. 81. Zheng J, Tung SL, Leung KY. 2005. Regulation of a type III and a putative secretion system in Edwardsiella tarda by EsrC is under the control of a two-component system, EsrA-EsrB. Infect Immun 73:4127-4137. 82. Suarez G, Sierra JC, Sha J, Wang S, Erova TE, Fadl AA, Foltz SM, Horneman AJ, Chopra AK. 2008. Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog 44:344-361. 83. Bernard CS, Brunet YR, Gavioli M, Lloubes R, Cascales E. 2011. Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins. J Bacteriol 193:2158-2167. 84. Kitaoka M, Miyata ST, Brooks TM, Unterweger D, Pukatzki S. 2011. VasH is a transcriptional regulator of the type VI secretion system functional in endemic and pandemic Vibrio cholerae. J Bacteriol 193:6471-6482. 85. Dobrindt U, Chowdary MG, Krumbholz G, Hacker J. 2010. Genome dynamics and its impact on evolution of Escherichia coli. Med Microbiol Immunol 199:145-154. 86. Domingues S, Harms K, Fricke WF, Johnsen PJ, da Silva GJ, Nielsen KM. 2012. Natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species. PLoS Pathog 8:e1002837. 87. Dorman CJ. 2009. Regulatory integration of horizontally-transferred genes in bacteria. Front Biosci (Landmark Ed) 14:4103-4112. 88. Fortier LC, Sekulovic O. 2013. Importance of prophages to evolution and virulence of bacterial pathogens. Virulence 4:354-365. 89. Wozniak RA, Waldor MK. 2010. Integrative and conjugative elements: mosaic mobile genetic elements enabling dynamic lateral gene flow. Nat Rev Microbiol 8:552-563. 90. Dorman CJ. 2013. Genome architecture and global gene regulation in bacteria: making progress towards a unified model? Nat Rev Microbiol 11:349-355. 91. Dorman CJ. 2004. H-NS: a universal regulator for a dynamic genome. Nat Rev Microbiol 2:391-400. 92. Fang FC, Rimsky S. 2008. New insights into transcriptional regulation by H-NS. Curr Opin Microbiol 11:113-120. 93. Lucchini S, Rowley G, Goldberg MD, Hurd D, Harrison M, Hinton JC. 2006. H-NS mediates the silencing of laterally acquired genes in bacteria. PLoS Pathog 2:e81. 94. Navarre WW, McClelland M, Libby SJ, Fang FC. 2007. Silencing of xenogeneic DNA by H-NS-facilitation of lateral gene transfer in bacteria by a defense system that recognizes foreign DNA. Genes Dev 21:1456-1471. 95. Oshima T, Ishikawa S, Kurokawa K, Aiba H, Ogasawara N. 2006. Escherichia coli histone-like protein H-NS preferentially binds to horizontally acquired DNA in association with RNA polymerase. DNA Res 13:141-153. 96. Liu Y, Chen H, Kenney LJ, Yan J. 2010. A divalent switch drives H-NS/DNA-binding conformations between stiffening and bridging modes. Genes Dev 24:339-344. 97. Navarre WW, Porwollik S, Wang Y, McClelland M, Rosen H, Libby SJ, Fang FC. 2006. Selective silencing of foreign DNA with low GC content by the H-NS protein in Salmonella. Science 313:236-238. 98. Eijkelkamp BA, Stroeher UH, Hassan KA, Elbourne LD, Paulsen IT, Brown MH. 2013. H-NS plays a role in expression of Acinetobacter baumannii virulence features. Infect Immun 81:2574-2583. 99. Salomon D, Klimko JA, Orth K. 2014. H-NS regulates the Vibrio parahaemolyticus type VI secretion system 1. Microbiology 160:1867-1873. 100. Zhang J, Xiao J, Zhang Y, Cui S, Liu Q, Wang Q, Wu H, Zhang Y. 2014. A new target for the old regulator: H-NS suppress T6SS secretory protein EvpP, the major virulence factor in the fish pathogen Edwardsiella tarda. Lett Appl Microbiol 59:557-564. 101. Bertin P, Benhabiles N, Krin E, Laurent-Winter C, Tendeng C, Turlin E, Thomas A, Danchin A, Brasseur R. 1999. The structural and functional organization of H-NS-like proteins is evolutionarily conserved in gram-negative bacteria. Mol Microbiol 31:319-329. 102. Bertin P, Hommais F, Krin E, Soutourina O, Tendeng C, Derzelle S, Danchin A. 2001. H-NS and H-NS-like proteins in Gram-negative bacteria and their multiple role in the regulation of bacterial metabolism. Biochimie 83:235-241. 103. Basler M, Ho BT, Mekalanos JJ. 2013. Tit-for-tat: type VI secretion system counterattack during bacterial cell-cell interactions. Cell 152:884-894. 104. Miyata ST, Kitaoka M, Brooks TM, McAuley SB, Pukatzki S. 2011. Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum. Infect Immun 79:2941-2949. 105. Arold ST, Leonard PG, Parkinson GN, Ladbury JE. 2010. H-NS forms a superhelical protein scaffold for DNA condensation. Proc Natl Acad Sci U S A 107:15728-15732. 106. Ono S, Goldberg MD, Olsson T, Esposito D, Hinton JC, Ladbury JE. 2005. H-NS is a part of a thermally controlled mechanism for bacterial gene regulation. Biochem J 391:203-213. 107. Stella S, Falconi M, Lammi M, Gualerzi CO, Pon CL. 2006. Environmental control of the in vivo oligomerization of nucleoid protein H-NS. J Mol Biol 355:169-174. 108. Aubert DF, Flannagan RS, Valvano MA. 2008. A novel sensor kinase-response regulator hybrid controls biofilm formation and type VI secretion system activity in Burkholderia cenocepacia. Infect Immun 76:1979-1991. 109. Chen Y, Wong J, Sun GW, Liu Y, Tan GY, Gan YH. 2011. Regulation of type VI secretion system during Burkholderia pseudomallei infection. Infect Immun 79:3064-3073. 110. Shiu SH, Karlowski WM, Pan R, Tzeng YH, Mayer KF, Li WH. 2004. Comparative analysis of the receptor-like kinase family in Arabidopsis and rice. Plant Cell 16:1220-1234. 111. Tendeng C, Badaut C, Krin E, Gounon P, Ngo S, Danchin A, Rimske S, Bertin P. 2000. Isolation and characterization of vicH, encoding a new pleiotropic regulator in Vibrio cholerae. Journal of Bacteriology 182:2026-2032. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52181 | - |
| dc.description.abstract | Pseudomonas syringae為一具極性鞭毛之革蘭氏桿狀菌，由於其寄主範圍廣泛且其寄主多具經濟價值，它已成為被研究最多的模式植物病原細菌之一。過去的研究指出，其病原性及致病力主要是由第三型分泌系統及其分泌之效應蛋白所掌控；然而我們的研究初步發現，第六型分泌系統在Pto DC3000 與其他微生物的競爭能力上扮演重要的角色，進而影響其在生態上的適應性。為了進一步了解第六型分泌系統所調控的功能，我們針對Pto DC3000第第六型分泌系統基因叢集 (gene cluster) 內的轉錄因子，PSPTO_5424 (sfa2)，進行了次世代轉錄體定序的分析，結果發現Sfa2僅調控少數基因，其中一個受到 Sfa2負向調控的基因為PSPTO_0281。PSPTO_0281的基因產物可能是 H-NS (Histone like nucleoid structuring protein)，推測可能為一轉錄抑制蛋白，可廣泛調控多種基因的表現，尤其是外來基因的表現情形。從qRT-PCR、GUS 活性測試與西方免疫墨點法的結果發現H-NS對hcp2基因或其蛋白質的表現與分泌並沒有太大的影響。但是在競爭實驗中，當大量表現PSPTO_0281時，Pto DC3000 對 E. coli MG1655及 P. syringae pv. phaseolicola 1448a的競爭能力會稍微降低。此外，前人研究發現第三型分泌系統與第六型分泌系統之間的調控機制往往伴隨著相互制衡的現象。因此我們進而探討PSPTO_0281與Sfa2是否對Pto DC3000第三型分泌系統有影響。在即時反轉錄聚合酶連鎖反應與GUS 活性測試的結果發現第三型分泌系統重要調控因子hrpR 與hrpL及效應蛋白之基因 avrPto的表現量在Δsfa2突變株及Δsfa2Δ0281雙突變株中均有升高的趨勢，顯示PSPTO_0281 和Sfa可能扮演負調控第三型分泌系統的角色。另一方面，Pto DC3000的病原性分析實驗中顯示，Δsfa2突變株或大量表現 PSPTO_0281 的菌株對於番茄的致病能力比野生株高，但在菸草中幾乎完全相反。這也表示了在不同的宿主植物中，病原菌所引起的反應可能有所差異。最後，我們也證明了 PSPTO_0281 確實是一個DNA 結合蛋白，然而與其結合的 DNA 序列並無專一性可。 | zh_TW |
| dc.description.abstract | Pseudomonas syringae is a rod-shaped, Gram-negative bacterium with polar flagella. As a plant pathogen with broad host range and economical importance, P. syringae has become one of the most studied model phytopathogen. From previous studies, we have known that type III secretion system (T3SS) plays a crucial role in the pathogenicity of P. syringae. In addition, the type VI secretion system (T6SS) has been shown to be involved in the fitness of interbacterial competition. By means of RNA sequencing to identify gene differentially expressed in P. syringae pv. tomato DC3000 wild type and Δsfa2, we discovered one gene, PSPTO_0281, which was highly expressed in Δsfa2. Although PSPTO_0281 was predicted to encode a putative histone-like nucleoid structuring (H-NS) protein, whether it acts as a transcriptional regulator remain to be elucidated. Using qRT-PCR, GUS activity and Western blot analysis, the expression and secretion of hcp2 were found independent of PSPTO_0281. However, overexpression of PSPTO_0281 slightly decreased the interbacterial competition ability of Pto DC3000 against E. coli MG1655 and P. syringae pv. phaseolicola 1448a. Considering the reciprocal regulation of T6SS and the T3SS in the gene regulatory network of Pto DC3000, T3SS-related genes were further analyzed. Indeed, PSPTO_0281 as well as Sfa2 both negatively regulate the expression of T3SS regulators and effectors, including hrpR, hrpL and avrPto. In the pathogenicity assay, overexpression of PSPTO_0281 enhanced virulence in Pto DC3000 as shown in Δsfa2 mutant. However, different results were obtained when Nicotiana benthamiana was used as host plants, suggesting different mechanisms were deployed in different plants in response to pathogen attack. Lastly, we also verified that PSPTO_0281 encodes a DNA-binding protein, which bind to DNA nonspecifically. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T16:09:08Z (GMT). No. of bitstreams: 1 ntu-104-R01623029-1.pdf: 26449388 bytes, checksum: 29827991eada2c4655c4f410aa967db2 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | Index
Acknowledgements ...………………………………………A English abstract ………………………………………………B Chinese abstract ………………………………………………D Introduction …………………………………………………………1 Motivation and Objectives ………………………14 Materials and Methods …………………………………15 Results ………………………………………………………………………29 Discussion ………………………………………………………………40 References ………………………………………………………………46 | |
| dc.language.iso | en | |
| dc.title | 植物病原菌Pseudomonas syringae pv. tomato DC3000中PSPTO_0281 基因之特性分析 | zh_TW |
| dc.title | Characterization of PSPTO_0281 gene in phytogenic Pseudomonas syringae pv. tomato DC3000 | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張晃猷,賴爾,鄧文玲 | |
| dc.subject.keyword | Pseudomonas syringae,第三型分泌系統,第六型分泌系統,Histone-like nucleoid structuring (H-NS) protein, | zh_TW |
| dc.subject.keyword | Pseudomonas syringae,type III secretion system (T3SS),type VI secretion system (T6SS),histone-like nucleoid structuring (H-NS) protein, | en |
| dc.relation.page | 89 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-08-19 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農業化學研究所 | zh_TW |
| 顯示於系所單位： | 農業化學系 | |
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