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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 洪挺軒 | |
dc.contributor.author | Yin-Fan Wang | en |
dc.contributor.author | 王映方 | zh_TW |
dc.date.accessioned | 2021-06-15T14:08:49Z | - |
dc.date.available | 2015-08-25 | |
dc.date.copyright | 2015-08-25 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-08-19 | |
dc.identifier.citation | Adams, M., Antoniw, J., & Fauquet, C. (2005). Molecular criteria for genus and species discrimination within the family Potyviridae. Arch Virol, 150(3), 459-479.
Adsuar, J. (1950). Studies on virus diseases of Papaya (Carica papaya) in Puerto Rico. IV. Preliminary studies on the host range of Papaya mosaic. Tech. Pap. PR agric. Exp. Stn.(5). Arbatova, J., Lehto, K., Pehu, E., & Pehu, T. (1998). Localization of the P1 protein of potato Y potyvirus in association with cytoplasmic inclusion bodies and in the cytoplasm of infected cells. J Gen Virol, 79 ( Pt 10), 2319-2323. Baker, C. A., Hiebert, E., Marlow, G. C., and Wisler, G. C. ( 1992. ). Comparative sequence analysis of the Reunion isolate of zucchini yellow mosaic virus. (Abstr.) Phytopathology 82:1176. Bateson, M. F., Lines, R. E., Revill, P., Chaleeprom, W., Ha, C. V., Gibbs, A. J., & Dale, J. L. (2002). On the evolution and molecular epidemiology of the potyvirus Papaya ringspot virus. Journal of General Virology, 83(10), 2575-2585. Boevink, P., & Oparka, K. J. (2005). Virus-host interactions during movement processes. Plant Physiology, 138(4), 1815-1821. Brantley, J. D., & Hunt, A. G. (1993). The N-terminal protein of the polyprotein encoded by the potyvirus tobacco vein mottling virus is an RNA-binding protein. J Gen Virol, 74 ( Pt 6), 1157-1162. Carrington, J. C., & Freed, D. D. (1990). Cap-independent enhancement of translation by a plant potyvirus 5'nontranslated region. J Virol, 64(4), 1590-1597. Carrington, J. C., & Herndon, K. L. (1992). Characterization of the potyviral HC-Pro autoproteolytic cleavage site. Virology, 187(1), 308-315. Chen, G., Ye, C., Huang, J., Yu, M., & Li, B. (2001). Cloning of the papaya ringspot virus (PRSV) replicase gene and generation of PRSV-resistant papayas through the introduction of the PRSV replicase gene. Plant Cell Reports, 20(3), 272-277. Chen, K.-C., Wang, C., Liu, F., Su, W.-C., & Yeh, S.-D. (2003). The NIa gene of Papaya ringspot virus is the host determinant for papaya infection. Paper presented at the 7th International Congress of Plant Molecular Biology. Barcelona, Spain. Chen, K. C., Chiang, C. H., Raja, J. A., Liu, F. L., Tai, C. H., & Yeh, S. D. (2008). A single amino acid of niapro of papaya ringspot virus determines host specificity for infection of papaya. Mol Plant Microbe Interact, 21(8), 1046-1057. doi: 10.1094/mpmi-21-8-1046 Chiang, C.-H., Lee, C.-Y., Wang, C.-H., Jan, F.-J., Lin, S.-S., Chen, T.-C., . . . Yeh, S.-D. (2007). Genetic analysis of an attenuated Papaya ringspot virus strain applied for cross-protection. European journal of plant pathology, 118(4), 333-348. Chung, B. Y.-W., Miller, W. A., Atkins, J. F., & Firth, A. E. (2008). An overlapping essential gene in the Potyviridae. Proceedings of the National Academy of Sciences, 105(15), 5897-5902. Chung, B. Y., Miller, W. A., Atkins, J. F., & Firth, A. E. (2008). An overlapping essential gene in the Potyviridae. Proc Natl Acad Sci U S A, 105(15), 5897-5902. doi: 10.1073/pnas.0800468105 Conover, R. A. ( 1964. ). Distortion ringspot, a severe virus disease of papaya in Florida. . Proc. Fla St. hort. Soc., 77:440-443. Cook, A. A. M. G. M. (1971). Virus diseases of papaya on Oahu (Hawaii) and identification of additional diagnostic host plants. Washington. Cotton, S., Grangeon, R., Thivierge, K., Mathieu, I., Ide, C., Wei, T., . . . Laliberté, J.-F. (2009). Turnip mosaic virus RNA replication complex vesicles are mobile, align with microfilaments, and are each derived from a single viral genome. J Virol, 83(20), 10460-10471. Cronin, S., Verchot, J., Haldeman-Cahill, R., Schaad, M. C., & Carrington, J. C. (1995). Long-distance movement factor: a transport function of the potyvirus helper component proteinase. The Plant Cell Online, 7(5), 549-559. Cui, X., Wei, T., Chowda-Reddy, R., Sun, G., & Wang, A. (2010). The Tobacco etch virus P3 protein forms mobile inclusions via the early secretory pathway and traffics along actin microfilaments. Virology, 397(1), 56-63. Dawn Parks, T., & Dougherty, W. G. (1991). Substrate recognition by the Nla proteinase of two potyviruses involves multiple domains: Characterization using genetically engineered hybrid proteinase molecules. Virology, 182(1), 17-27. De Bokx, J. (1965). Hosts and electron microscopy of two Papaya viruses. Plant Disease Reporter, 49(9), 742-746. De La Rosa, M., Lastra,R. (1983). Purification and patial characterization of Papaya ring spot virus. Phytopathologische Zeitschrift 106:329-336. Desbiez, C., & Lecoq, H. (2004). The nucleotide sequence of Watermelon mosaic virus (WMV, Potyvirus) reveals interspecific recombination between two related potyviruses in the 5' part of the genome. Arch Virol, 149(8), 1619-1632. doi: 10.1007/s00705-004-0340-9 Dolja, V., Herndon, K., Pirone, T., & Carrington, J. (1993). Spontaneous mutagenesis of a plant potyvirus genome after insertion of a foreign gene. J Virol, 67(10), 5968-5975. Dougherty, W. G., & Carrington, J. C. (1988). Expression and function of potyviral gene products. Annual Review of phytopathology, 26(1), 123-143. Dunoyer, P., Thomas, C., Harrison, S., Revers, F., & Maule, A. (2004). A cysteine-rich plant protein potentiates Potyvirus movement through an interaction with the virus genome-linked protein VPg. J Virol, 78(5), 2301-2309. E. Maiss, U. T., A. Brisske, W. Jelkmann, R. Casper, G. Himmler, D. Mattanovich, and H. W. D. Katinger. ( 1989 ). The Complete Nucleotide Sequence of Plum Pox Virus RNA J Gen Virol March 70:513-524; doi:10.1099/0022-1317-70-3-513. Edwardson, J., Christie, R., & Ko, N. (1984). Potyvirus cylindrical inclusions-- Subdivision-IV. Phytopathology, 74(9), 1111-1114. Eiamtanasate, S., Juricek, M., & Yap, Y.-K. (2007). C-terminal hydrophobic region leads PRSV P3 protein to endoplasmic reticulum. Virus genes, 35(3), 611-617. Endres, M. W., Gregory, B. D., Gao, Z., Foreman, A. W., Mlotshwa, S., Ge, X., . . . Vance, V. (2010). Two plant viral suppressors of silencing require the ethylene-inducible host transcription factor RAV2 to block RNA silencing. PLoS Pathog, 6(1), e1000729. Ferreira, S., Pitz, K., Manshardt, R., Zee, F., Fitch, M., & Gonsalves, D. (2002). Virus coat protein transgenic papaya provides practical control of papaya ringspot virus in Hawaii. Plant Disease, 86(2), 101-105. Gal-On, A. (2000). A point mutation in the FRNK motif of the potyvirus helper component-protease gene alters symptom expression in cucurbits and elicits protection against the severe homologous virus. Phytopathology, 90(5), 467-473. Gibbs, A. J., Ohshima, K., Phillips, M. J., & Gibbs, M. J. (2008). The prehistory of potyviruses: their initial radiation was during the dawn of agriculture. PLoS One, 3(6), e2523. Gonsalves, D., & Ishii, M. (1980). Purification and serology of papaya ringspot virus. Phytopathology, 70(11), 1028-1032. Gough, K. H., & Shukla, D. D. (1993). Nucleotide sequence of Johnsongrass mosaic potyvirus genomic RNA. Intervirology, 36(3), 181-192. Greber, R. S. (1978). Watermelon mosaic virus 1 and 2 in Queensland cucurbit crops. Aust. J. agric. Ris. 29:1235-1245. Guo, D., Rajamäki, M.-L., Saarma, M., & Valkonen, J. P. (2001). Towards a protein interaction map of potyviruses: protein interaction matrixes of two potyviruses based on the yeast two-hybrid system. Journal of General Virology, 82(4), 935-939. Hung, T. H., Wu, M. L. and Su, H. J. . (1999). Development of A Rapid Method for the Diagnosis of Citrus Greening Disease using the Polymerase Chain Reaction. Journal of Phytopathology, . 147: 599–604. doi: 110.1046/j.1439-0434.1999.00435. Hung, T. H., Wu, M.L., Su, H.J. . (2000). A rapid method based on the one-step reverse transcriptase-polymerase chain reaction (RT-PCR) technique for detection of different strains of Citrus Tristeza Virus. J., Phytopathol. 148:469-475. Jakab, G., Droz, E., Brigneti, G., Baulcombe, D., & Malnoe, P. (1997). Infectious in vivo and in vitro transcripts from a full-length cDNA clone of PVY-N605, a Swiss necrotic isolate of potato virus Y. J Gen Virol, 78 ( Pt 12), 3141-3145. Jensen, D. D. (1949). Papaya virus diseases with special reference to Papaya ringspot. Phytopathology, 39(3), 191-211. Kasschau, K. D., & Carrington, J. C. (1998). A counterdefensive strategy of plant viruses: suppression of posttranscriptional gene silencing. Cell, 95(4), 461-470. Kasschau, K. D., Cronin, S., & Carrington, J. C. (1997). Genome amplification and long-distance movement functions associated with the central domain of tobacco etch potyvirus helper component–proteinase. Virology, 228(2), 251-262. Kasschau, K. D., Xie, Z., Allen, E., Llave, C., Chapman, E. J., Krizan, K. A., & Carrington, J. C. (2003). P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA unction. Dev Cell, 4(2), 205-217. Kawarasaki, Y., Kasahara, S., Kodera, N., Shinbata, T., Sekiguchi, S., Nakano, H., & Yamane, T. (2000). A trimmed viral cap-independent translation enhancing sequence for rapid in vitro gene expression. Biotechnol Prog, 16(3), 517-521. doi: 10.1021/bp000021x Klein, P. G., Klein, R. R., Rodriguez-Cerezo, E., Hunt, A. G., & Shaw, J. G. (1994). Mutational analysis of the tobacco vein mottling virus genome. Virology, 204(2), 759-769. Klein, P. G., Klein, R. R., Rodriguez-Cerezo, E., Hunt, A. G., & Shaw, J. G. (1994). Mutational analysis of the tobacco vein mottling virus genome. Virology, 204(2), 759-769. doi: 10.1006/viro.1994.1591 Lain, S., Martin, M., Riechmann, J., & Garcia, J. (1991). Novel catalytic activity associated with positive-strand RNA virus infection: nucleic acid-stimulated ATPase activity of the plum pox potyvirus helicaselike protein. J Virol, 65(1), 1-6. Langenberg, W. G., & Zhang, L. (1997). Immunocytology shows the presence of tobacco etch virus P3 protein in nuclear inclusions. Journal of structural biology, 118(3), 243-247. Li, X. H., Valdez, P., Olvera, R. E., & Carrington, J. C. (1997). Functions of the tobacco etch virus RNA polymerase (NIb): subcellular transport and protein-protein interaction with VPg/proteinase (NIa). J Virol, 71(2), 1598-1607. Lin, L., Luo, Z., Yan, F., Lu, Y., Zheng, H., & Chen, J. (2011). Interaction between potyvirus P3 and ribulose-1, 5-bisphosphate carboxylase/oxygenase (RubisCO) of host plants. Virus genes, 43(1), 90-92. Lindner, R. C., Jensen, D. D., and Ikeda, W. (1945.). Ringspot: new papaya plunderer. Hawaii Farm and Home 8:10-14. Mallory, A. C., Reinhart, B. J., Bartel, D., Vance, V. B., & Bowman, L. H. (2002). A viral suppressor of RNA silencing differentially regulates the accumulation of short interfering RNAs and micro-RNAs in tobacco. Proc Natl Acad Sci U S A, 99(23), 15228-15233. doi: 10.1073/pnas.232434999 Mallory, A. C., Reinhart, B. J., Bartel, D., Vance, V. B., & Bowman, L. H. (2002). A viral suppressor of RNA silencing differentially regulates the accumulation of short interfering RNAs and micro-RNAs in tobacco. Proceedings of the National Academy of Sciences, 99(23), 15228-15233. Mangrauthia, S. K., Parameswari, B., Jain, R., & Praveen, S. (2008). Role of genetic recombination in the molecular architecture of Papaya ringspot virus. Biochemical genetics, 46(11-12), 835-846. Michon, T., Estevez, Y., Walter, J., German‐Retana, S., & Gall, O. (2006). The potyviral virus genome‐linked protein VPg forms a ternary complex with the eukaryotic initiation factors eIF4E and eIF4G and reduces eIF4E affinity for a mRNA cap analogue. Febs Journal, 273(6), 1312-1322. Milne, K. S., Grogan, R.G., Kimble, K.A. . (1969). Identification of virus infecting cucurbits in California. Phytopathology 59:819-828. Miyoshi, H., Okade, H., Muto, S., Suehiro, N., Nakashima, H., Tomoo, K., & Natsuaki, T. (2008). < i> Turnip mosaic virus</i> VPg interacts with< i> Arabidopsis thaliana</i> eIF (iso) 4E and inhibits< i> in vitro</i> translation. Biochimie, 90(10), 1427-1434. Moreno, M., Bernal, J., Jim, I., & Rodr, E. (1998). Resistance in plants transformed with the P1 or P3 gene of tobacco vein mottling potyvirus. Journal of General Virology, 79(11), 2819-2827. Moury, B. t., Morel, C., Johansen, E., & Jacquemond, M. (2002). Evidence for diversifying selection in Potato virus Y and in the coat protein of other potyviruses. Journal of General Virology, 83(10), 2563-2573. MURPHY, J. F., KLEIN, P. G., HUNT, A. G., & SHAW, J. G. (1996). Replacement of the tyrosine residue that links a potyviral VPg to the viral RNA is lethal. Virology, 220(2), 535-538. Niepel, M., & Gallie, D. R. (1999). Identification and characterization of the functional elements within the tobacco etch virus 5′ leader required for cap-independent translation. J Virol, 73(11), 9080-9088. Noa-Carrazana, J. C., González-de-León, D., & Silva-Rosales, L. (2007). Molecular characterization of a severe isolate of papaya ringspot virus in Mexico and its relationship with other isolates. Virus genes, 35(1), 109-117. Paalme, V., Gammelgard, E., Jarvekulg, L., & Valkonen, J. P. (2004). In vitro recombinants of two nearly identical potyviral isolates express novel virulence and symptom phenotypes in plants. J Gen Virol, 85(Pt 3), 739-747. Parameswari, B., Mangrauthia, S. K., Praveen, S., & Jain, R. K. (2007). Complete genome sequence of an isolate of Papaya ringspot virus from India. Arch Virol, 152(4), 843-845. doi: 10.1007/s00705-006-0890-0 Parris, G. K. (1938). A new disease of papaya. Purcifull, D., & Edwardson, J. (1967). Watermelon mosaic virus: Tubular inclusions in pumpkin leaves and aggregates in leaf extracts. Virology, 32(3), 393-401. Purcifull, D. E., Edwardson, J., and Gonsalves, D. . (1984. ). Papaya ringspot virus. C.M.I./A.A.B. Descriptions of Plant Viruses, No. 292. Puustinen, P., & Mäkinen, K. (2004). Uridylylation of the potyvirus VPg by viral replicase NIb correlates with the nucleotide binding capacity of VPg. Journal of Biological Chemistry, 279(37), 38103-38110. Quiot-Douine, L., Purcifull, D., Hiebert, E., & De Mejia, M. (1986). Serological relationships and in vitro translation of an antigenically distinct strain of papaya ringspot virus. Phytopathology, 76(3), 346-351. Rajamäki, M.-L., Kelloniemi, J., Alminaite, A., Kekarainen, T., Rabenstein, F., & Valkonen, J. P. T. (2005). A novel insertion site inside the potyvirus P1 cistron allows expression of heterologous proteins and suggests some P1 functions. Virology, 342(1), 88-101. doi: http://dx.doi.org/10.1016/j.virol.2005.07.019 Rajamäki, M.-L., & Valkonen, J. P. (2009). Control of nuclear and nucleolar localization of nuclear inclusion protein a of picorna-like Potato virus A in Nicotiana species. The Plant Cell Online, 21(8), 2485-2502. Rao, M. B., Tanksale, A. M., Ghatge, M. S., & Deshpande, V. V. (1998). Molecular and Biotechnological Aspects of Microbial Proteases. Microbiology and Molecular Biology Reviews, 62(3), 597-635. Riechmann, J. L., Cervera, M. T., & García, J. A. (1995). Processing of the plum pox virus polyprotein at the P3-6K1 junction is not required for virus viability. Journal of General Virology, 76(4), 951-956. Riechmann, J. L., Lain, S., & Garcia, J. A. (1992). Highlights and prospects of potyvirus molecular biology. J Gen Virol, 73 ( Pt 1), 1-16. Roberts, I. M., Wang, D., Findlay, K., & Maule, A. J. (1998). Ultrastructural and temporal observations of the potyvirus cylindrical inclusions (Cls) show that the Cl protein acts transiently in aiding virus movement. Virology, 245(1), 173-181. Rodriguez-Cerezo, E., Ammar, E., Pirone, T., & Shaw, J. (1993). Association of the non-structural P3 viral protein with cylindrical inclusions in potyvirus-infected cells. Journal of General Virology, 74, 1945-1945. Rodriguez-Cerezo, E., & Shaw, J. G. (1991). Two newly detected nonstructural viral proteins in potyvirus-infected cells. Virology, 185(2), 572-579. Rohozkova, J., & Navratil, M. (2011). P1 peptidase--a mysterious protein of family Potyviridae. J Biosci, 36(1), 189-200. Rojas, M. R., Zerbini, F. M., Allison, R. F., Gilbertson, R. L., & Lucas, W. J. (1997). Capsid protein and helper component-proteinase function as potyvirus cell-to-cell movement proteins. Virology, 237(2), 283-295. Russo, M., Martelli, G.P., Vovlas, C., Ragozzino, A. . (1979). Comparative studies on Mediterranean isolates of Watermelon mosaic virurs. Phytopathol. Mediterr. 18:94-101. Sáenz, P., Cervera, M. T., Dallot, S., Quiot, L., Quiot, J.-B., Riechmann, J. L., & Garcı́a, J. A. (2000). Identification of a pathogenicity determinant of Plum pox virus in the sequence encoding the C-terminal region of protein P3+ 6K1. Journal of General Virology, 81(3), 557-566. Sakai, J., Mori, M., Morishita, T., Tanaka, M., Hanada, K., Usugi, T., & Nishiguchi, M. (1997). Complete nucleotide sequence and genome organization of sweet potato feathery mottle virus (S strain) genomic RNA: the large coding region of the P1 gene. Arch Virol, 142(8), 1553-1562. Schaad, M. C., Jensen, P. E., & Carrington, J. C. (1997). Formation of plant RNA virus replication complexes on membranes: role of an endoplasmic reticulum‐targeted viral protein. The EMBO journal, 16(13), 4049-4059. Shen, W., Yan, P., Gao, L., Pan, X., Wu, J., & Zhou, P. (2010). Helper component‐proteinase (HC‐Pro) protein of Papaya ringspot virus interacts with papaya calreticulin. Molecular plant pathology, 11(3), 335-346. Shi, F., Gao, F., Shen, J., Chang, F., & Zhan, J. (2014). Sequence variation of P1 gene in Potato virus Y isolated from Fujian Province. Yi Chuan, 36(7), 713-722. doi: 10.3724/sp.j.1005.2014.0713 Shiboleth, Y. M., Haronsky, E., Leibman, D., Arazi, T., Wassenegger, M., Whitham, S. A., . . . Gal-On, A. (2007). The Conserved FRNK Box in HC-Pro, a Plant Viral Suppressor of Gene Silencing, Is Required for Small RNA Binding and Mediates Symptom Development. Journal of Virology, 81(23), 13135-13148. doi: 10.1128/JVI.01031-07 Shiboleth, Y. M., Haronsky, E., Leibman, D., Arazi, T., Wassenegger, M., Whitham, S. A., . . . Gal-On, A. (2007). The conserved FRNK box in HC-Pro, a plant viral suppressor of gene silencing, is required for small RNA binding and mediates symptom development. J Virol, 81(23), 13135-13148. doi: 10.1128/JVI.01031-07 Soumounou, Y., & Laliberte, J. F. (1994). Nucleic acid-binding properties of the P1 protein of turnip mosaic potyvirus produced in Escherichia coli. J Gen Virol, 75 ( Pt 10), 2567-2573. Stenger, D. C., Hall, J. S., Choi, I.-R., & French, R. (1998). Phylogenetic relationships within the family Potyviridae: wheat streak mosaic virus and brome streak mosaic virus are not members of the genus Rymovirus. Phytopathology, 88(8), 782-787. Story, G. E., Halliwell, R.S. . (1969). Identification of distortion ringspot virus disease of papaya in the Domincan Republic. Plant Disease Reptr. 53:757-760. Tatineni, S., Qu, F., Li, R., Morris, T. J., & French, R. (2012). Triticum mosaic poacevirus enlists P1 rather than HC-Pro to suppress RNA silencing-mediated host defense. Virology, 433(1), 104-115. Tavert-Roudet, G., Abdul-Razzak, A., Doublet, B., Walter, J., Delaunay, T., German-Retana, S., . . . Candresse, T. (2012). The C terminus of lettuce mosaic potyvirus cylindrical inclusion helicase interacts with the viral VPg and with lettuce translation eukaryotic initiation factor 4E. J Gen Virol, 93(Pt 1), 184-193. doi: 10.1099/vir.0.035881-0 Tena Fernandez, F., Gonzalez, I., Doblas, P., Rodriguez, C., Sahana, N., Kaur, H., . . . Canto, T. (2013). The influence of cis-acting P1 protein and translational elements on the expression of Potato virus Y helper-component proteinase (HCPro) in heterologous systems and its suppression of silencing activity. Mol Plant Pathol, 14(5), 530-541. doi: 10.1111/mpp.12025 Urcuqui-Inchima, S., Maia, I. G., Arruda, P., Haenni, A.-L., & Bernardi, F. (2000). Deletion Mapping of the Potyviral Helper Component-Proteinase Reveals Two Regions Involved in RNA Binding. Virology, 268(1), 104-111. doi: http://dx.doi.org/10.1006/viro.1999.0156 Valli, A., Martin-Hernandez, A. M., Lopez-Moya, J. J., & Garcia, J. A. (2006). RNA silencing suppression by a second copy of the P1 serine protease of Cucumber vein yellowing ipomovirus, a member of the family Potyviridae that lacks the cysteine protease HCPro. J Virol, 80(20), 10055-10063. doi: 10.1128/jvi.00985-06 Verchot, J., & Carrington, J. C. (1995a). Debilitation of plant potyvirus infectivity by P1 proteinase-inactivating mutations and restoration by second-site modifications. J Virol, 69(3), 1582-1590. Verchot, J., & Carrington, J. C. (1995b). Evidence that the potyvirus P1 proteinase functions in trans as an accessory factor for genome amplification. J Virol, 69(6), 3668-3674. Verchot, J., Herndon, K. L., & Carrington, J. C. (1992). Mutational analysis of the tobacco etch potyviral 35-kDa proteinase: identification of essential residues and requirements for autoproteolysis. Virology, 190(1), 298-306. Wang, X., Ullah, Z., & Grumet, R. (2000). Interaction between zucchini yellow mosaic potyvirus RNA-dependent RNA polymerase and host poly-(A) binding protein. Virology, 275(2), 433-443. Ward, C. W., & Shukla, D. D. (1991). Taxonomy of potyviruses: current problems and some solutions. Intervirology, 32(5), 269-296. Webb, R. E., Bohn, GW., Scott, H.A. . (1965). Watermelon mosaic virus 1 and 2 in southern and western cucurbit production areas. Plant Disease Reptr. 49:532-535. Wei, T., Huang, T.-S., McNeil, J., Laliberté, J.-F., Hong, J., Nelson, R. S., & Wang, A. (2010). Sequential recruitment of the endoplasmic reticulum and chloroplasts for plant potyvirus replication. J Virol, 84(2), 799-809. Yeh, S.-D., & Gonsalves, D. (1994). Practices and perspective of control of papaya ringspot virus by cross protection Advances in disease vector research (pp. 237-257): Springer. Yeh, S., Gonsalves, D., & Provvidenti, R. (1984). Comparative studies on host range and serology of papaya ringspot virus and watermelon mosaic virus 1. Phytopathology, 74(9), 1081-1085. Yeh, S. D., & Gonsalves, D. (1985). Translation of papaya ringspot virus RNA in vitro: detection of a possible polyprotein that is processed for capsid protein, cylindrical-inclusion protein, and amorphous-inclusion protein. Virology, 143(1), 260-271. Yeh, S. D., Jan, F. J., Chiang, C. H., Doong, T. J., Chen, M. C., Chung, P. H., & Bau, H. J. (1992). Complete nucleotide sequence and genetic organization of papaya ringspot virus RNA. J Gen Virol, 73 ( Pt 10), 2531-2541. Yu, B., Chapman, E. J., Yang, Z., Carrington, J. C., & Chen, X. (2006). Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis. FEBS Letters, 580(13), 3117-3120. doi: http://dx.doi.org/10.1016/j.febslet.2006.04.063 Zeenko, V., & Gallie, D. R. (2005). Cap-independent translation of tobacco etch virus is conferred by an RNA pseudoknot in the 5'-leader. J Biol Chem, 280(29), 26813-26824. doi: 10.1074/jbc.M503576200 Zettler, F., Edwardson, J., & Purcifull, D. (1968). Ultramicroscopic differences in inclusions of papaya mosaic virus and papaya ringspot virus correlated with differential aphid transmission. Phytopathology, 58(3), 332-335. 木瓜輪點病毒系統之細胞病理學研究. (1988). National Taiwan University Department of Plant Pathology and Entomology. 王惠亮、王金池、邱人璋、孫明賢. (1978). 臺灣木瓜輪點病研究初報 植物保護學會會刊 20:133~140. 吉井三惠子. (1986). 影響木瓜輪點病毒病徵表現與變異之因素: National Taiwan University Dept. of Plant Pathology . 李宜霞. (2006). 木瓜輪點病毒之Real-time RT-PCR定量偵測技術研發與應用. 110. 李宜霞, & Lee, Y.-S. 木瓜輪點病毒之Real-Time RT-PCR定量偵測技術之研發與應用 Development and Application of the Quantitative Detection of Papaya ringspot potyvirus Based on Real-Time RT-PCR Technique. 林正忠. (1980). 木瓜輪點病病毒之系統及交叉保護: National Taiwan University Department of Plant Pathology and Entomology. 翁芬華. (1981a). 木瓜輪點病毒之變異性. 翁芬華. (1981b). 木瓜輪點病毒之變異性: National Taiwan University Department of Plant Pathology and Entomology. 張仲萍. (2013). 木瓜輪點病毒超強株系P1和HC-Pro基因在基因沉寂抑制作用之交互功能. 陳冠君. (2007). 木瓜輪點病毒感染木瓜及其毒力相關基因之分析. 陳脈紀、劉顯達、王惠亮、位國慶、邱人璋. (1976). 木瓜輪點病之電子顯微鏡觀察. 植物保護學會會刊論文摘要, 18:399. 楊瑞春. (2012). 木瓜輪點病毒嚴重嵌紋壞疽系統及嚴重嵌紋系統感染性選殖株之構築及不同系統間之比較. 廖奕晴. (2004). 台灣木瓜輪點病毒系統之變異與鑑別及快速偵測. 臺灣大學. Available from Airiti AiritiLibrary database. (2004年) 蔡文惠. (1995). 木瓜接種不同輪點病毒系統後的反應: National Taiwan University Dept. of Horticulture. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52103 | - |
dc.description.abstract | 木瓜為台灣重要果樹,感染木瓜之病害中以木瓜輪點病毒(Papaya ring spot virus, PRSV)引起的木瓜輪點病(Papaya ring spot)最具破壞性。此病毒屬於馬鈴薯Y病毒科(Potyviridae),馬鈴薯Y病毒屬(Potyvirus),使寄主葉片產生嵌紋、變形甚至絲狀化、果實表面出現水浸狀輪斑等病徵。PRSV主要分為P型及W型,P型可感染番木瓜科及葫蘆科,W型只能感染葫蘆科。PRSV-P型目前依照葉片上呈現之病徵又分為嚴重嵌紋(severe mottling, SM)、嚴重嵌紋壞疽(severe mottling with necrosis, SMN)與畸形(deformation, DF)三個主要系統。三系統間具有病理性及分子性之差異,顯示PRSV田間演化情況複雜,目前田間病害以SMN與DF兩系統為主導優勢,兩系統基因序列相似度極高病徵卻截然不同,值得探入探討造成病徵不同之決定因子(symptom determinants)。SMN系統與DF系統全長序列相似度為96.5%,各基因核苷酸序列相似度約為96-99%,靠近5’端的序列相似度較低,如P3基因相似度95.8-96.7%,P1基因低於95%,5’UTR則在93.0-94.2%,本論文將以實驗室SMN與DF兩系統具感染力之全長cDNA選殖株,選擇兩系統間差異性較大之P1基因序列進行重組置換。以精確phusion PCR增幅DF系統之5’端至P1的基因片段,後接SMN的片段,構築成5’-DF(P1)-SMN的重組cDNA選殖株 ; 也以同樣的方法構築5’端至P1為SMN片段,後接DF片段另一重組5’-SMN(P1)-DF選殖株。以胞外轉錄(in vitro transcription)將兩感染性選殖株合成完整的病毒基因體RNA transcripts,機械接種於最感病的木瓜寄主台農二號,發現5’-DF(P1)-SMN重組選殖株的病徵表現以DF為主,5’-SMN(P1)-DF重組選殖株亦然,顯示P1基因對病徵有影響,但不是唯一的病徵決定因子。 | zh_TW |
dc.description.abstract | Papaya ring spot caused by Papaya ring spot virus (PRSV) , one of the most destructive diseases in papaya. PRSV belonging to the genus Potyvirus, family Potyviridae. PRSV-infected papaya trees show mosaic, distorted and shoestring-like symptoms on the leaves, and sunken ring spots on the fruits. PRSV categorized into 2 types, PRSV-P type and PRSV-W type. The P type infects both papayas and cucurbits whereas the W type only infects cucurbits. Based on the incited symptoms, the PRSV-P type further devided into SM (severe mottling), SMN (severe mottling with necrosis) and DF (deformation) strains. Between these 3 strains have apparently differences on pathological and molecular. This study investigate the genomic variations and find the key genomic areas associated with pathogenicity among different PRSV strains through the research with artificially recombinant infectious clones infecting papaya hosts. Based on the previously constructed infectious clones of PRSV SMN and DF strains, two new recombinant infectious named 5’-DF(P1)-SMN and 5’-SMN(P1)-DF clones were further made. The 5’-DF(P1)-SMN clone has a head of DF (5’UTR and P1 fragment of DF) followed by the SMN fragment, and the 5’-SMN(P1)-DF clone has a head of SMN (5’UTR and P1 fragment of SMN) followed by the DF fragment. The recombinant genomic RNA trascripts were synthesized through in vitro transcription, and they were used to individually inoculate the TN2 papayas. The results showed that symptom express of both two recombinant clones similar to PRSV-DF. It revealed that P1 gene contribute part of pathogenicity, but not the only one gene affect symptom express. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T14:08:49Z (GMT). No. of bitstreams: 1 ntu-104-R01633022-1.pdf: 5634563 bytes, checksum: 8233608dc2a93c7a60f4c6350d63b8a3 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 目錄
壹、前言 1 貳、前人研究 3 一、木瓜輪點病之起源與危害 3 二、木瓜輪點病毒分類與分子特性 4 三、木瓜輪點病毒之內含體 4 參、材料與方法 15 一、試驗植物準備 15 二、病毒來源 15 三、人工重組感染性選殖株構築 15 1. PCR (使用phusionTMHigh-Fidelity DNA Polymerase) 15 2. PCR (使用LA TaqTM PCR polymerase) 15 3. 酒精沉澱法 16 4. 接合作用(Ligation) 16 5. 電穿孔 16 6. 篩選並定序確認 16 7. 微量質體純化 17 四、胞外轉錄(in vitro transcription) 17 五、接種試驗 18 六、木瓜輪點病毒之偵測方法 18 1. 病毒核酸萃取 18 2. Two-Step RT-PCR 18 3. One-Step RT-PCR 19 4. PCR產物電泳膠體分析 19 七、菌株繼代培養 21 1. 轉型作用(transformation) 21 陸、參考文獻 33 柒、圖與表 45 捌、附錄 70 表目錄 表一、木瓜輪點病毒重組選殖株(Papaya ringspot virus recombinants)基因核酸片段之構築所用引子對及其基因產物 46 表二、木瓜輪點病毒重組選殖株(Papaya ringspot virus recombinants)基因核酸片段之定序所用引子對及其基因產物 47 表三、以反轉錄聚合酶連鎖反應(RT-PCR)與即時定量反轉錄聚合酶連鎖反應(real-time RT-PCR)偵測木瓜輪點病毒(PRSV)所使用引子及探針 48 表四、病徵整理比較 49 表五、人工構築5’-DF(P1)-SMN、5’-SMN(P1)-DF與DF、SMN之P1核酸和胺基酸序列相似度和歧異度比對 50 表六、接種前5’-DF(P1)-SMN、5’-SMN(P1)-DF與接種後5’-DF(P1)-SMN、5’-SMN(P1)-DF之P1核酸和安基酸序列相似度和歧異度比對 51 圖目錄 圖一、木瓜輪點病毒(PRSV)人工重組P1片段之選殖策略 52 圖二、構築5’-DF(P1)-SMN infectious clone 53 圖三、構築5’-SMN(P1)-DF infectious clone 54 圖四、重組人工質體全長基因體cDNA胞外轉錄後以XbaI酵解為線狀 56 圖五、人工重組後木瓜輪點病毒(PRSV)全長cDNA選殖株之胞外轉錄RNA產物電泳分析 57 圖六、人工重組選殖株DF、5’-DF(P1)-SMN、SMN及5’-SMN(P1)-DF接種後以通用性引子對PRSV-476、DF626、SMN466進行RT-PCR偵測 58 圖十三、台農二號接種5’-SMN(P1)-DF選殖株胞外轉錄產物30天、60天、90天、100天後病徵表現 65 圖十四、5’-SMN(P1)-DF繼代接種在台農二號木瓜上引起之病徵 66 圖十五、台農二號接種DF選殖株胞外轉錄產物30天、60天、90天、100天後病徵表現 67 | |
dc.language.iso | zh-TW | |
dc.title | 以人工重組感染性選殖株探討木瓜輪點病毒基因變異
對致病性之影響 | zh_TW |
dc.title | The study of genomic variation of Papaya ring spot virus
(PRSV) affecting its pathogenicity with artificially recombinant infectious clones | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 沈湯龍,陳穎練 | |
dc.subject.keyword | 木瓜輪點病毒,轉基因,感染性選殖株,P1基因, | zh_TW |
dc.subject.keyword | Papaya ring spot virus,transgenic papaya,infectious clone,P1 gene, | en |
dc.relation.page | 74 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2015-08-19 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
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