請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51958
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 黃鵬林(Pung-Ling Huang) | |
dc.contributor.author | Yu-Cheng Ko | en |
dc.contributor.author | 柯昱成 | zh_TW |
dc.date.accessioned | 2021-06-15T13:59:39Z | - |
dc.date.available | 2020-08-25 | |
dc.date.copyright | 2015-08-25 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-08-20 | |
dc.identifier.citation | 洪福佑. 2010. 抗喜姆比蘭嵌紋病毒與齒舌蘭輪斑病毒之基因默化轉殖菸草抗病效力分析.國立臺灣大學園藝學研究所碩士論文. 徐善德. 2007.蝴蝶蘭癒合組織再生植株與轉殖體系之建立.國立臺灣大學園藝學研究所博士論文. 張紹強. 2011.香蕉抗萎縮病及ACC氧化酶基因默化轉殖株分析. 國立臺灣大學園藝學研究所碩士論文 葉素瑛. 2004. 蘭花病毒專一性載體構築與暫時性表達分析.國立臺灣大 學園藝學研究所碩士論文. 劉賢玲. 2000. 臺灣蝴蝶蘭之病毒病因研究. 國立臺灣大學植物病理學研究所碩士論文. 楊玉婷. 2010. 全球蘭花發展現況與未來展望. 臺灣經濟研究. 33: 36-41. 張清安.1996. 蘭花病毒的特性與防治. 農業世界. 151:14-19. Ajjikuttira, P. A., C. L. Lim-Ho, M. H. Woon, K. H. Ryu, C. A. Chang, C. S. Loh, and S. M. Wong. 2002. Genetic variability in the coat protein genes of two orchid virus: Cymbidium mosaic virus and Odontoglossum ring spot virus. Arch. Virol. 147: 1943-1954. Arag atilde;o, F. J. L., L. M. G Barros, A. C. M.. Brasileiro, S. G. Ribeiro, F. D. Smith, J. C. Sanford, J. C. Fari,aand E. L. Rech.1996. Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment. Theor. Appl. Genet. 93:142-150. Baulcombe, D.2004. RNA silencing in plants. Nature 431:356-363. Baulcombe, D. C. 1999. Fast forward genetics based on virus-induced gene silencing. Curr. Opin. Plant Bio. 2: 109-113. Baulcombe, D. C. 1996. Mechanisms of pathogen-derived resistance to virus in transgenic plants. Plant Cell 8:1833-1844. Beachy, R. N. 1997. Mechanisms and applications of pathogen-derived resistance in transgenic plants. Curr. Opin. Bio. 8:215-220. Casas A.M., A. K Kononowicz, R.A Bressan, and P.M. Hasegawa 1995. Cereal transformation through particle bombardment. Plant Breed Rev. 13:235–264. Chan, S.W.L., I. R. Henderson,. and S. E. Jacobsen. 2005. Gardening the genome: DNA methylation in Arabidopsis thaliana. Nat. Rev. Genet. 6,351–360. Chandler, S. F. and F. Brugliera. 2011. Genetic modification in floriculture. Biotechnol. Lett. 33:207-214. Chapman, E.J., and J.C. Carrington. 2007. Specialization and evolution of endogenous small RNA pathways. Nat. Rev. Genet. 8:884–896. Cheng, M., Z. Li, J. W. Demski, and R. L. Jarret.1997. Expression and inheritance of foreign genes in transgenic peanut plants generated by Agrobacterium-mediated transformation. Plant Cell Rep.16:541-544. Chng, C. G., S. M. Wong, P. H. Mahtani, C. S. Loh, C. J. Goh, M. C. C. Kao, M. C. M. Chung, and Y. Watanabe. 1996. The complete sequence of a Singapore isolate of odontoglossum ringspot virus and comparison with other tobamoviruses. Gene 171: 155-161. Catoni, M., A. Lucioli, P. Doblas-Ibanez, G. P. Accotto, and A. M. Vaira. 2013. From immunity to susceptibility: virus resistance induced in tomato by silenced transgene is lost as TGS overcome PTGS. Plant J. 75:941-953. Christou, P., W. F. Swain, N. Yang, and D. E. McCabe.1989. Inheritance and expression of foreign genes in transgenic soybean plants. Proc. Natl. Acad. Sci. USA 86: 7500-7504. Covey, S. N., N. S. Al-Kaff, A. Langara, and D.S. Turner. 1997. Plants combat infection by gene silencing. Nature 385:781-782. Datta S.K., A. Peterhans, K. Datta, and I. Potrykus.1990. Genetically engineered fertile indica-rice recovered from protoplasts. BioTechnology 8:736–740. Dawson, W. O. 1996. Gene silencing and virus resistance: a common mechanism. Trends Plant Sci. 1:107-108. Demeke, T., P. Hucl, M. Baga, K. Caswell, N. Leung, and R. N. Chibbar. 1999. Transgene inheritance and silencing in hexaploid spring wheat. Theor. Appl. Genet 99: 947-953. Deroles, S. C., and R. C. Gardner. 1988. Expression and inheritance of kanamycin resistance in a large number of transgenic petunias generated by Agrobacterium-mediated transformation. Plant Mol. Bio. 11: 355-364. Dillen, W, J. De Clercq, A. Goossens, M. Van Montagu, and G. Angenon. 1997. Agrobacterium-media-ted transformation of Phaseolus acutifolius A. Gray. Theor. Appl. Genet. 94:151–158. Donald, R. G. K., and A. O. Jackson. 1994. The barely stripe mosaic virus rb gene encodes a multifunctional cysteine-rich protein that affects pathogenesis. Plant Cell 6: 1593-1606. Duan, X., X. Li, Q. Xue, M. Abo-El-Saad, D. Xu , and R. Wu.1996. Transgenic rice plants harbouring an introduced potato proteinase inhibitor II gene are insect resistant. Nature Biotechnol. 14:494–498. Duncan, R.R. 1997. Tissue culture-induced variation and crop improvement. Adv. Agron. 58: 201–240. Earle, E.B. and V. E. Gracen. 1985. Somaclonal variation in progeny of plants from corn tissue culture. In: R. Henke et al. (Eds.) Progagation of Higher Plants through Tissue Culture, Plenum, New York, pp. 139–152. Elmayan, T.,and H. Vaucheret. 1996. Expression of single copies of a strongly expressed 35 S transgene can be silenced post-transcriptionally. Plant J. 9:787–797. Fearing, P. L., D. Brown, D. Vlachos, M. Meghji, L. Privalle. 1997. Quantitative analysis of CryIA(b) protein expression in Bt maize plants, tissues, and silage and stability of expression over successive generations. Mol. Breed. 3:169–176. Feinberg, A. P., and B. Vogelstein. 1983. A technique for radio labeling DNA restriction endonucluease fragments to high specific activity. Anal. Biochem. 132:6-13. Finnegan, J., and D. McElory.1994. Transgene inactivation: plants fight back. Bio. Technol. 12: 883-888. Fu, D.Q., B. Z. Zhu, H.L. Zhu, H.X. Zhang, Y. H. Xie, W. B. Jiang, X. D. Zhao, and Y. B. Luo.2006. Enhancement of virus-induced gene silencing in tomato by low temperature and low humidity. Mol. Cells 21:153–160. Fuentes, A., P. L. Ramos, E. Fiallo, D. Callard, Y. Sanchez, R. Peral, R. Rodriguez, and M. Pujol. 2006. Intron-hairpin RNA derived from replication associated protein C1 gene confers immunity to Tomato Yellow Leaf Curl Virus infection in transgenic tomato plants. Transgenic Res. 15:291-304. Fulton, R. W. 1986. Practices and precautions in the use of cross protection for plant virus disease control. Ann. Rev. Phytopathology 24 :67-81. Golemboski, D. B., G. P. Lomonossoff, and M.. Zaitlin. 1990. Plants transformed with a tobacco mosaic virus nonstructural gene sequence are resistant to the virus. Proc. Natll Acad. Sci. 87:6311-6315. Goldbach, R., E. Bucher, and M. Prins. 2003. Resistance mechanisms to plant virus: an overview. Virus Res. 92:207-212. Goto, T., S. Komochi, N. Oshima. 1966. Study on control of plant virus diseases by vaccination with attenuated virus (2). Effects of concentration and time elapsed after inoculation of tomato with attenuated TMV against infection with virulent parent strain. Ann. Phytopathol. Soc. Jpn. 32:221-226. Hannon, G.J. 2002. RNA interference. Nature 418:244–251. Hamilton, A.J. and Baulcombe, D.C. 1999. A species of small antisense RNA in posttranscriptional gene silencing in plants. Science 286: 950–952. Hammond, S. M., E. Bernstein, D. Beach,and G. J. Hannon. 2000. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 404:293-296. Helliwell, C. A., and P. M. Waterhouse, 2005. Constructs and methods for hairpin RNA-mediated gene silencing in plants. Methods enzymol. 392: 24-35. Hiei, Y., S. Ohta, T. Komari, and T. Kumashiro. 1994. Efficient transformation rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J. 6: 271-282. Hily, J. M., M. Ravelonandro, V. Damsteegt, C. Bassett, Z. Liu, and R. Scorza. 2007. Plum pox virus coat protein gene Intron-hairpin-RNA (ihpRNA) constructs provide resistance to plum pox virus in Nicotiana benthamiana and Prunus domestica. J. Amer. Soc. Hort. Sci. 132:850-858. Hobbs, S. L. A., T. D. Warkentin, M.O. DeLong. 1993. Transgene copy number can be positively or negatively associated with transgene expression. Plant Mol. Biol. 21:17–26. Hu, Q., Y. Niu, K. Zhang, Y. Liu, and X. Zhou. 2011. Virus-derived transgenes expressing hairpin RNA give immunity to Tobacco mosaic virus and Cucumber mosaic virus. Virol. J. 8:41-51. Hu, W. W., S. M. Wong, C. S. Loh, and C. J. Goh. 1998. Synergism in replication of cymbidium mosaic potexvirus (CymMV) and odontoglossum ringspot tobamovirus (ORSV) RNA in orchid protoplasts. Arch. Virol. 143: 1265-1275. Jayaraman, K., S. A. Fingran, J. Shah, and J. Fyles. 1991. Polymeras chain reaction-mediated gene synthesis: synthesis of a gene coding for isozyme C of horseradish peroxidase. Pro. Natl. Acad. Sci. USA. 88:4084-4088. Jensen, D. D. 1951. Mosaic or black streak disease of Cymbidium orchids. Phytopathology 41: 401-414. Jensen, D. D., and A. H. Gold. 1951. A virus ring spot of Odontoglossum Orchid: symptoms, transmission, and electron microscopy. Phytopathology 41: 648-653. Johnson, J. 1937. An acquired partial immunity to the tobacco streak disease. Trans. Wis. Acad. Sci. Arts Lett. 30:27-34. Jofuku, K. D., and R. B. Goldberg. 1988. Analysis of plant gene structure. p37-42. In: Shaw, C. H. (ed). Plant Biology. A practical approach. IRL. Press, Oxford, UK. Kaeppler, S. M., H. F. Kaeppler,and Y. Rhee. 2000. Epigenetic aspects of somaclonal variation in plants. Plant Mol. Bio. 43: 179-188. Kalantidis, K., S. Psaradakis, M. Tabler, and M. Tsagris. 2002. The occurrence of CMV-specific short RNAs in transgenic tobacco expressing virus-derived double stranded RNA is indicative of resistance to the virus. Mol. Plant- Microbe Interact. 15: 826-833. Kilby, N., H. M. O. Leyser,and I. J.Furner. 1992. Promoter methylation and progressive transgene inactivation in Arabidopsis. Plant Mol. Biol. 20:103–112. Kumagai, M. H., J. Donson, G. Della-Cioppa, D. Harvey, K. Hanley, L. K. Grill. 1995.Cytoplasmic inhibition of carotenoid biosynthesis with virus derived RNA. Proc. Natl. Acad. Sci. USA 92:1679-1683. Liao, L. J., I. C. Pan, Y. L. Chan, Y.H. Hsu, W. H. Chen, and M. T. Chan. 2004.Transgene silencing in Phalaenopsis expressing the coat protein of Cymbidium mosaic virus is a manifestation of RNA-mediated resistence. Mol. Breeding 13: 229-242. Matzke, M.and A. J. M. Matzke. 1995. How and why do plants inactivate homologous (trans)genes? Plant Physiol. 107:679–685. Matzke, M., and A. Matzke. 1991. Differential inactivation and methylation of a transgene in plant by two suppressor loci containing homologous sequences. Plant Mol. Biol. 16, 821–830. Meyer, P., F. Linn. I. Heidmann, H. Meyer, I. Niedenhof, and H. Saedler. 1992. Endogenous and environmental factors influence 35S promoter methylation of a maize A1 gene construct in transgenic petunia and its colour phenotype. Mol. Gen. Genet. 231: 345–352. Miki, D., and K. Shimamoto. 2004. Simple RNAi vectors for stable and transient suppression of gene function in rice. Plant Cell Physiol. 45: 490–495. Mitra, R., K. Krishnamurthy, E. Blancaflor, M. Payton, R. S. Nelson, and J. Verchot-Lubicz. 2003. The Potato virus X TGBp2 protein association with the endoplasmic reticulum plays a role in but is not sufficient for viral cell -to-cell movement. Virology 312:35-48. Mittelsten-Scheid, O., L. Jakovleva, K. Afsar, J. Maluszynska, and J. Paszkwoski.1996. A change of ploidy can modify epigenetic silencing. Proc. Natl. Acad. Sci. USA 93:7114–7119. Morozov, S. Yu.and A. G. Solovyev. 1999. Genome organization in RNA viruses. In Molecular Biology of Plant Viruses, pp. 47–98. Edited by C. L. Mandahar. Boston/Dordrecht/London: Kluwer. M uuml;ller, E., H. L ouml;rz, S.L uuml;tticke. 1996. Variability of transgene expression in clonal cell lines of wheat. Plant Sci. 114:71–82. Paludan, N. 1976. Tobacco mosaic virus (TMV): Effects of infection with strains of TMV on yields of unprotected tomato plants and of plants protected with attenuated strains of TMV as compared to varieties resistant to TMV. Tidsskr. Planteav 80: 189-203. Peng, J., F. Wen., R.L. Lister, T.K. Hodges.1995. Inheritance of gusA and neo genes in transgenic rice. Plant Mol. Biol. 27:871–884. Potrykus, I., J. Paszkowski, M. W. Saul, J. Petruska, and R. D. Shillito. 1985. Molecular and general genetics of a hybrid forgien gene introduced into tobacco by direct gene transfer. Mol. Gen. Genet. 199:169-177. Powell-Abel, P. , R. S. Nelson, B. De, N. Hoffman, S. G. Rogers, R.T. Fraley, and R. N. Beachy. 1986. Delay disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Sience 232:738-743. Ratcliff, F., M. H. Ana Montserrat, and C.B. David. 2001. Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant J. 25: 237–245. Ratcliff, F., B. D. Harrison, and D.C. Baulcombe.1997. A similarity between viral defense and gene silencing in plants. Science 276:1558-1560. Rathore K.S., V. K. Chowdhury, T. K. Hodges.1993. Use of bar as a selectable marker gene and for the production of herbicide-resistant rice plants from protoplasts. Plant Mol. Bio. l21:871–884. Robertson, D. 2004. VIGS vectors for gene silencing: many targets, many tools. Annu. Rev. Plant Biol. 55: 495–519. Ryu, K. H., and W. M. Park. 1995. The complete nucleotide sequence and genome organization of odontoglossum ring spot tobamovirus RNA. Arch. Virol. 140: 1577-1587. Ryu, C. M., A. Ajith, K. Li, and K.S. Mysore. 2004. Agrodrench: a novel and effective agroinoculation method for virus-induced gene silencing in roots and diverse Solanaceous species. Plant J. 40: 322–331. Sachs, E.S., J. H. Benedict, D.M Stelly., J. F. Tayor, D.W Altman., S.A. Berberich, S.K. Davis.1998. Expression and segregation of genes encoding cryIA insecticidal proteins in cotton. Crop Sci.38:1–11. Sambrook, P. N. , G. D. Champion, C. D. Browne, D. Cairns, M. L. Cohen, R. O. Day, S. Graham, M. Handel, R. Jaworski, and S. Kempler. 1989. Corticosteroid injection for osteoarthritis of the knee: peripatellar compared to intra-articular route. Clin. Exp. Rheumatol. 7: 609-613. Senthil‐Kumar, M., and K. S. Mysore. 2011. Virus‐induced gene silencing can persist for more than 2 years and also be transmitted to progeny seedlings in Nicotiana benthamiana and tomato. Plant biotechnology J. 9: 797-806. Scheid, O. M., J. Paszkowski and I. Potrykus. 1991. Reversible inactivation of a transgene in Arabidopsis thaliana. Mol. Gen. Genet. 228, 104–112. Schwind, N., M. Zwiebel, A. Itaya, B. Ding, M. B. Wang, G. Krczal, and M. Wassenegger. 2009. RNAi-mediated resistance to potato spindle tuber viroid in transgenic tomato expressing a viroid hairpin RNA construct. Mol. Plant Pathol. 10:459-469. Scott, A., D. Woodfield, D.W. R.White.1998. Allelic composition and genetic background effects on transgene expression and inheritance in white clover. Mol. Breed. 4:479–490. Shimamoto K, R. Terada, T. Izawa, H. Fujimoto.1989. Fertile transgenic rice plants regenerated from transformed protoplasts. Nature 338:274–276. Smith, N. A., S. P. Singh, M. B. Wang, P. A.Stoutjesdijk, A. G. Green,and P. M. Waterhouse. 2000. Gene expression: Total silencing by intron-spliced hairpin RNAs. Nature 407: 319-320. Southern, E. M. 1975. Detection of specific sequence among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503-517. Spencer, T. M., J.V. O’Brien, W.G. Start, T.R. Adams.1992. Segregation of transgene in maize. Plant Mol. Biol. 18:201–210. Srivastava, V., V. Vasil ,and I. K. Vasil. 1996. Molecular characterization of the fate of transgenes in transformed wheat (Triticum aestivum L.). Theor. Appl. Genet. 92:1031–1037. Stoger, E., S. Williams, D. Kenn,and P. Christou.1999. Constitutive versus seed specific expression in transgenic wheat: temporal and spatial control. Transgenic Res. 8:73–82 Tuttle, J. R., A. M. Idris, J. K. Brown, C. H. Haigler, and D. Robertson. 2008. Geminivirus-mediated gene silencing from Cotton leaf crumple virus is enhanced by low temperature in cotton. Plant Physiol. 148:41–50. Ulian, E.C., J. M. Magill, and R. H. Smith.1994. Expression and inheritance pattern of two foreign genes in petunia. Theor. Appl. Genet 88: 433-440. Van Kammen, A.1997. Virus-induced gene silencing in infected and transgenic plants. Trends Plant Sci. 2:409-411. Vazquez, F. 2006. Arabidopsis endogenous small RNAs: highways and byways. Trends Plant Sci. 11:460–468. Vejaratpimol, R., C. Channuntapipat, P. Liewsaree, T. Pewnim, K. Ito, M. Iizuka,and N. Minamiura. 1998. Evaluation of enzyme-linked Immunosorbent assays for the dection of Cymbidium mosaic virus in orchid. J. Ferment. Bioeng. 86: 65-71. Verchot-Lubicz, J. 2005. A new cell to cell transport model for potexviruses. Mol. Plant Microbe Interact. 18: 283–290. Voinnet, O. 2001. RNA silencing as a plant immune system against viruses. Trends Genet. 17: 449–459. Walters, D. A., C.S. Vetsch, D.E. Potts, R.C. Lundquist.1992. Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants. Plant Mol. Biol. 18:189–200. Wang, H. L., S. D. Yeh, R. J. Chiu, and D. Gonsalves. 1987. Effectiveness of cross-protection by mild mutants of papaya ringspot virus for control of ringspot disease of papaya in Taiwan. Plant disease 71 : 491-497. Wang, M. B., D. C. Abbot, and P. M. Waterhouse. 2000. A single copy of a virus-derived transgene encoding hairpin RNA gives immunity to barley yellow dwarf virus. Mol. Plant Pathol. 1:347-356. Watson, J.M., A. F. Fusaro, M.Wang, and P. M.Waterhouse. 2005. RNA silencing platforms in plants. FEBS Lett. 579:5982–5987. Wesley, S. V., C. A. Helliwell, N. A. Smith, M. Wang, D. T. Rouse, Q. Liu, P. S. Gooding, S. P. Singh, D. Abbott, P. A. Stoutjesdijk, S. P. Robinson, A. P. Gleave, A. G. Green, and P. M. Waterhouse. 2001. Construct design for efficient, effective and high‐throughput gene silencing in plants. Plant J. 27:581-590. Wong, S. M., C. G. Chang, Y. H. Lee, K. Tan, F. W. Zettler. 1994. Incidence of cymbidium mosaic and odontoglossum ring spot virus and their significance in orchid cultivation in Singapore. Crop. Prot. 13: 235-239. Wong, S. M., P. H. Mahtani, K. C. Lee, H. H. Yu, Y. Tan, K. K. Neo, Y. Chan, M. Wu, and C. G.. Chng. 1997. Cymbidium mosaic potexvirus RNA: complete nucleotide sequence and phylogenetic analysis. Arch. Virol. 142: 383-391. Wu, G., H. Cui, G. Ye, Y. Xia, R. Sardana, X.Cheng, Y. Li, I. Altosaar, and Q. Shu. 2002. Inheritance and expression of the cry1Ab gene in Bt (Bacillus thuringiensis) transgenic rice. Theoretical and Applied Genetics 104:727-734. Yeh, S. D., and D. Gonsalves. 1984. Evaluation of induced mutants of papaya ringspot virus for control by cross protection. Phytopathology 74:1086-1091. Zettler, F. W., N. J. Ko, G.. C. Wisler, M. S. Elliot, and S. M. Wong. 1990. Virus of Orchid and their control. Plant Dis. 74: 621-626. Zettler, F. W., G.. C.Wisler, M. S. Ettiot, and N. J. Ko. 1987. Some new, potentially significant viruses of orchids and their probable means of transmission. Am, Orchid Soc. Bull 56: 1044-1051. Zhang, S., D. Warkentin, B. Sun, H. Zhong, M. Sticklen.1996. Variation in the inheritance of expression among subclones for unselected (uidA) and selected (bar) transgenes in maize (Zea mays L.). Theor. Appl. Genet. 92:752–761. Zhang, H., and J. K. Zhu. 2011. RNA-directed DNA methylation. Curr. Opin. Plant Biol. 14, 142–147. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51958 | - |
dc.description.abstract | 蝴蝶蘭是臺灣重要的花卉,然而在栽培過程中,經常受到病毒害感染,造成植株生長不良、喪失商品價值,常見的病毒病害以蕙蘭嵌紋病毒(Cymbidium mosaic virus; CymMV)和齒舌蘭輪斑病毒 (Odontoglossum ringspot virus; ORSV)感染最為普遍。本論文所分析的雜交後代,母本為含CymmV外鞘蛋白基因默化構築之蝴蝶蘭轉殖株,或含CymMV外鞘蛋白基因默化構築之癒傷組織再轉殖ORSV外鞘蛋白基因默化構築所得之轉殖株,父本為未轉殖的商業栽培品種,期望透過短干擾RNA (small interfering RNA, siRNA)之產生,降解病毒外鞘蛋白基因mRNA,達到抗病效果。
GUS (β—glucuronidase)活性組織化學染色結果顯示,兩個轉殖系之雜交後代GUS呈現正反應者占50%,符合遺傳分離率。經由聚合酶連鎖反應 (polymerase chain reaction; PCR)和南方氏雜交分析,確認CymMV和ORSV外鞘蛋白基因雙重默化轉殖株之雜交後代,約35%植株同時具有CymMV和ORSV外鞘蛋白默化轉殖基因。部分雜交後代經病毒接種試驗顯示具有抗病性。 | zh_TW |
dc.description.abstract | The Phalaenopsis orchids are important flowers in Taiwan. However, Phalaenopsis are usually infected by virus during cultivation and become growth retardation and loss of commercial vaule. The most common virus diseases are Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV). To utilize biotechnology on virus resistance for Phalaenopsis, RNA interference (RNAi) construct against coat proteim gene of CymMV was transformed into Phalaenopsis callus, and continued transformed with ORSV coat protein RNAi construct in previous studies. Phalaenopsis transformants used as maternal parent were cross-hybridized with untransformed commercial variety and progeny obtained were analyzed in this study. This construction can produce small interfering RNA (siRNA), make virus mRNA degradation then against virus infection.
The β—glucuronidase (GUS) staing results showed that, about 50% of hybrid progeny expressed GUS activity, which was followed Mendelian segregation ratio. Transgene inheritance in GUS positive hybrid progeny was confirmed by both polymerase chain reaction and Southern analysis. After virus inoculation on several individuals of hybrid progeny by RNAi construct and accumulation of virus coat protein was inhibited plantets showed resistance to virus. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T13:59:39Z (GMT). No. of bitstreams: 1 ntu-104-R00628130-1.pdf: 2115670 bytes, checksum: dca67fc96e64607b573d5dd861bd3463 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 壹、前言............................................................7 貳、前人研究........................................................8 一、蝴蝶蘭病毒簡介..............................................8 二、植物抗病毒之研究............................................10 (一)病毒交叉保護系統......................................10 (二)轉殖病毒外鞘蛋白基因於植物抗病毒之研究.............11 (三)轉殖複製酶基因於植物抗病毒之研究...................11 (四)轉殖運移蛋白基因於植物抗病毒之研究.................12 三、基因默化的機制和應用....................................12 (一)基因默化機制.......................................12 (二)RNAi構築於抗病毒之研究............................14 (三)病毒載體誘導的基因默化.............................16 四、外源轉殖基因遺傳之研究..................................17 (一)轉殖單一外源基因...................................17 (二)轉殖兩種以上外源基因...............................18 參、材料與方法................................................21 一、試驗材料................................................21 (一)質體材料...........................................21 (二)植物材料...........................................21 (三)植物病毒來源.......................................21 二、試驗方法................................................24 (一)植物基因組DNA之抽取..............................24 (二)GUS組織活性化學染色法............................24 (三)聚合酶連鎖反應....................................25 (四)南方氏雜交分析.. ...................................25 (五)探針製備...........................................26 (六)質體DNA小量製備..................................27 (七) DNA片段回收......................................28 (八)植物可溶性蛋白萃取.................................28 (九)蝴蝶蘭病毒接種.....................................28 (十)酵素連結免疫吸附法................................29 (十一)質體DNA轉形....................................30 (十二) DNA定序........................................30 肆、結果......................................................31 一、GUS活性組織化學染色分析...............................31 二、CymMV外鞘蛋白基因默化轉殖株雜交後代聚合酶連鎖反應.. ..31 三、CymMV外鞘蛋白基因默化轉殖株雜交後代南方氏雜交分析....,31 四、CymMV外鞘蛋白基因默化轉殖株雜交後代病毒接種分析.......38 五、蝴蝶蘭外鞘蛋白CymMV和ORSV外鞘蛋白雙重基因默化轉殖株雜 交後代聚合酶連鎖反應................................... 38 六、蝴蝶蘭外鞘蛋白CymMV和ORSV外鞘蛋白雙重基因默化轉殖株雜交後代南方氏雜交分析....................................38 七、蝴蝶蘭外鞘蛋白CymMV和ORSV外鞘蛋白雙重基因默化轉殖株雜交後代病毒接種分析......................................42 伍、討論..................................................... 48 一、轉殖單一外源基因於蝴蝶蘭雜交後代之遺傳情況............48 二、轉殖兩種外源基因於蝴蝶蘭雜交後代之遺傳情況.............49 三、蝴蝶蘭轉殖株雜交後代病毒接種...........................50 陸、結語......................................................58 柒、參考文獻..................................................59 | |
dc.language.iso | zh-TW | |
dc.title | 蝴蝶蘭轉殖株雜交後代分析之研究 | zh_TW |
dc.title | Studies on Hybrid Progenies of Transgenic Phalaenopsis Plants | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 杜宜殷(Yi-Yin Do) | |
dc.contributor.oralexamcommittee | 廖芳心(Fang-Shin Liao),徐善德(Shan-Te Hsu) | |
dc.subject.keyword | 蝴蝶蘭,病毒外鞘蛋白基因,RNAi構築,雜交,病毒接種, | zh_TW |
dc.subject.keyword | Phalaenopsis,virus coat protein gene,RNAi construct,Cross,virus inoculation, | en |
dc.relation.page | 72 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2015-08-20 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 園藝暨景觀學系 | zh_TW |
顯示於系所單位: | 園藝暨景觀學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-104-1.pdf 目前未授權公開取用 | 2.07 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。