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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊健志 | |
dc.contributor.author | Yi-Lin Hsieh | en |
dc.contributor.author | 謝毅霖 | zh_TW |
dc.date.accessioned | 2021-06-15T13:48:59Z | - |
dc.date.available | 2015-12-01 | |
dc.date.copyright | 2015-12-01 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-10-27 | |
dc.identifier.citation | Abeles, F., Morgan, P., and Saltveit, M. (1992). Ethylene in plant biology. (San Diego, CA: Academic Press.).
Alonso, J.M., Hirayama, T., Roman, G., Nourizadeh, S., and Ecker, J.R. (1999). EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis. Science 284, 2148-2152. An, F., Zhao, Q., Ji, Y., Li, W., Jiang, Z., Yu, X., Zhang, C., Han, Y., He, W., Liu, Y., Zhang, S., Ecker, J.R., and Guo, H. (2010). Ethylene-induced stabilization of ETHYLENE INSENSITIVE3 and EIN3-LIKE1 is mediated by proteasomal degradation of EIN3 binding F-box 1 and 2 that requires EIN2 in Arabidopsis. Plant Cell. 22, 2384-2401. Arc, E., Sechet, J., Corbineau, F., Rajjou, L., and Marion-Poll, A. (2013). ABA crosstalk with ethylene and nitric oxide in seed dormancy and germination. Frontiers in plant science 4, article 63. Attwood, P.V. (2013). Histidine kinases from bacteria to humans. Biochem Soc Trans. 41, 1023-1028. Attwood, P.V., Piggott, M.J., Zu, X.L., and Besant, P.G. (2007). Focus on phosphohistidine. Amino Acids. 32, 145-156. Beaudoin, N., Serizet, C., Gosti, F., and Giraudat, J. (2000). Interactions between abscisic acid and ethylene signaling cascades. Plant Cell. 12, 1103-1115. Benschop, J.J., Millenaar, F.F., Smeets, M.E., van Zanten, M., Voesenek, L.A., and Peeters, A.J. (2007). Abscisic acid antagonizes ethylene-induced hyponastic growth in Arabidopsis. Plant Physiol. 143, 1013-1023. Besant, P.G., and Attwood, P.V. (2009). Detection and analysis of protein histidine phosphorylation. Mol Cell Biochem. 329, 93-106. Bisson, M.M., and Groth, G. (2010). New insight in ethylene signaling: autokinase activity of ETR1 modulates the interaction of receptors and EIN2. Mol Plant 3, 882-889. Bisson, M.M., Bleckmann, A., Allekotte, S., and Groth, G. (2009). EIN2, the central regulator of ethylene signalling, is localized at the ER membrane where it interacts with the ethylene receptor ETR1. Biochem J 424, 1-6. Bleecker, A.B., and Kende, H. (2000). Ethylene: a gaseous signal molecule in plants. Annu Rev Cell Dev Biol 16, 1-18. Bleecker, A.B., Estelle, M.A., Somerville, C., and Kende, H. (1988). Insensitivity to ethylene conferred by a dominant mutation in Arabidopsis thaliana. Science 241, 1086-1089. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72, 248-254. Cao, Y.R., Chen, H.W., Li, Z.G., Tao, J.J., Ma, B., Zhang, W.K., Chen, S.Y., and Zhang, J.S. (2015). Tobacco ankyrin protein NEIP2 interacts with ethylene receptor NTHK1 and regulates plant growth and stress responses. Plant Cell Physiol. 56, 803-818. Chang, C., Kwok, S.F., Bleecker, A.B., and Meyerowitz, E.M. (1993). Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators. Science 262, 539-544. Chao, Q., Rothenberg, M., Solano, R., Roman, G., Terzaghi, W., and Ecker, J.R. (1997). Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHYLENE-INSENSITIVE3 and related proteins. Cell 89, 1133-1144. Chen, Y.F., Randlett, M.D., Findell, J.L., and Schaller, G.E. (2002). Localization of the ethylene receptor ETR1 to the endoplasmic reticulum of Arabidopsis. J Biol Chem 277, 19861-19866. Chen, Y.F., Shakeel, S.N., Bowers, J., Zhao, X.C., Etheridge, N., and Schaller, G.E. (2007). Ligand-induced degradation of the ethylene receptor ETR2 through a proteasome-dependent pathway in Arabidopsis. J Biol Chem 282, 24752-24758. Chiu, W.B., Lin, C.H., Chang, C.J., Hsieh, M.H., and Wang, A.Y. (2006). Molecular characterization and expression of four cDNAs encoding sucrose synthase from green bamboo Bambusa oldhamii. New Phytol 170, 53-63. Cho, E., Um, Y., Yoo, S., Lee, H., Kim, H., Koh, S., Shin, H., and Lee, Y. (2011). An expressed sequence tag analysis for the fast-growing shoots of Bambusa edulis Murno. J. Plant Biol. 54, 402-408. Clark, K.L., Larsen, P.B., Wang, X., and Chang, C. (1998). Association of the Arabidopsis CTR1 Raf-like kinase with the ETR1 and ERS ethylene receptors. Proc Natl Acad Sci U S A 95, 5401-5406. Duclos, B., Marcandier, S., Cozzone, A.J., Tony, H., and Bartholomew, M.S. (1991). Chemical properties and separation of phosphoamino acids by thin-layer chromatography and/or electrophoresis. In Methods in Enzymology (Academic Press), pp. 10-21. Gallivan, J.P., and Dougherty, D.A. (1999). Cation-p interactions in structural biology. Proc Natl Acad Sci U S A. 96, 9459-9464. Gamble, R.L., Coonfield, M.L., and Schaller, G.E. (1998). Histidine kinase activity of the ETR1 ethylene receptor from Arabidopsis. Proc Natl Acad Sci U S A 95, 7825-7829. Gao, Z., Chen, Y.F., Randlett, M.D., Zhao, X.C., Findell, J.L., Kieber, J.J., and Schaller, G.E. (2003). Localization of the Raf-like kinase CTR1 to the endoplasmic reticulum of Arabidopsis through participation in ethylene receptor signaling complexes. J Biol Chem 278, 34725-34732. Gao, Z., Wen, C.-K., Binder, B.M., Chen, Y.-F., Chang, J., Chiang, Y.-H., Kerris, R.J., III, Chang, C., and Schaller, G.E. (2008). Heteromeric interactions among ethylene receptors mediate signaling in Arabidopsis. J. Biol Chem 283, 23801-23810 Ghassemian, M., Nambara, E., Cutler, S., Kawaide, H., Kamiya, Y., and McCourt, P. (2000). Regulation of abscisic acid signaling by the ethylene response pathway in Arabidopsis. Plant Cell. 12, 1117-1126. Gunawardena, J. (2005). Multisite protein phosphorylation makes a good threshold but can be a poor switch. Proc Natl Acad Sci U S A. 102, 14617-14622. Guo, H., and Ecker, J.R. (2004). The ethylene signaling pathway: new insights. Curr Opin Plant Biol 7, 40-49. Guzman, P., and Ecker, J.R. (1990). Exploiting the triple response of Arabidopsis to identify ethylene-related mutants. Plant Cell. 2, 513-523. Hall, B.P., Shakeel, S.N., Amir, M., Ul Haq, N., Qu, X., and Schaller, G.E. (2012). Histidine kinase activity of the ethylene receptor ETR1 facilitates the ethylene response in Arabidopsis. Plant Physiol. 159, 682-695. Hamilton, A.J., Lycett, G.W., and Grierson, D. (1990). Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants. Nature 346, 284-287. Hansen, M., Chae, H.S., and Kieber, J.J. (2009). Regulation of ACS protein stability by cytokinin and brassinosteroid. Plant J. 57, 606-614. Hirayama, T., Kieber, J.J., Hirayama, N., Kogan, M., Guzman, P., Nourizadeh, S., Alonso, J.M., Dailey, W.P., Dancis, A., and Ecker, J.R. (1999). RESPONSIVE-TO-ANTAGONIST1, a Menkes/Wilson disease-related copper transporter, is required for ethylene signaling in Arabidopsis. Cell 97, 383-393. Hua, J., and Meyerowitz, E.M. (1998). Ethylene responses are negatively regulated by a receptor gene family in Arabidopsis thaliana. Cell 94, 261-271. Hua, J., Chang, C., Sun, Q., and Meyerowitz, E.M. (1995). Ethylene insensitivity conferred by Arabidopsis ERS gene. Science 269, 1712-1714. Hua, J., Sakai, H., Nourizadeh, S., Chen, Q.G., Bleecker, A.B., Ecker, J.R., and Meyerowitz, E.M. (1998). EIN4 and ERS2 are members of the putative ethylene receptor gene family in Arabidopsis. Plant Cell. 10, 1321-1332. Huang, Y., Li, H., Hutchison, C.E., Laskey, J., and Kieber, J.J. (2003). Biochemical and functional analysis of CTR1, a protein kinase that negatively regulates ethylene signaling in Arabidopsis. Plant J 33, 221-233. Hwang, I., and Sheen, J. (2001). Two-component circuitry in Arabidopsis cytokinin signal transduction. Nature. 413, 383-389. Hyodo, H., and Yang, S.F. (1971). Ethylene-enhanced synthesis of phenylalanine ammonia lyase in pea seedlings. Plant Physiol. 47, 765-770. Ju, C., Yoon, G.M., Shemansky, J.M., Lin, D.Y., Ying, Z.I., Chang, J., Garrett, W.M., Kessenbrock, M., Groth, G., Tucker, M.L., Cooper, B., Kieber, J.J., and Chang, C. (2012). CTR1 phosphorylates the central regulator EIN2 to control ethylene hormone signaling from the ER membrane to the nucleus in Arabidopsis. Proc Natl Acad Sci U S A. 109, 19486-19491. Kamiyoshihara, Y., Tieman, D.M., Huber, D.J., and Klee, H.J. (2012). Ligand-induced alterations in the phosphorylation state of ethylene receptors in tomato fruit. Plant Physiol. 160, 488-497. Kieber, J.J., Rothenberg, M., Roman, G., Feldmann, K.A., and Ecker, J.R. (1993). CTR1, a negative regulator of the ethylene response pathway in arabidopsis, encodes a member of the Raf family of protein kinases. Cell 72, 427-441. Konishi, M., and Yanagisawa, S. (2008). Ethylene signaling in Arabidopsis involves feedback regulation via the elaborate control of EBF2 expression by EIN3. Plant J 55, 821-831. Ku, H.S., Suge, H., Rappaport, L., and Pratt, H.K. (1970). Stimulation of rice coleoptile growth by ethylene. Planta. 90, 333-339. Kyte, J., and Doolittle, R.F. (1982). A simple method for displaying the hydropathic character of a protein. J Mol Biol. 157, 105-132. Lacey, R.F., and Binder, B.M. (2014). How plants sense ethylene gas-the ethylene receptors. J Inorg Biochem. 133, 58-62. Lin, C.S., Kalpana, K., Chang, W.C., and Lin, N.S. (2007). Improving multiple shoot proliferation in bamboo mosaic virus-free Bambusa oldhamii Munro propagation by liquid culture. Hortscience 42, 1243-1246. Lin, W.C. (1958). Studies on the growth of bamboo species in Taiwan. Bulletin of Taiwan Forestry Research Institute Vol. 54. Liu, Y., and Zhang, S. (2004). Phosphorylation of 1-aminocyclopropane-1-carboxylic acid synthase by MPK6, a stress-responsive mitogen-activated protein kinase, induces ethylene biosynthesis in Arabidopsis. Plant Cell. 16, 3386-3399. Lorenzo, O., Piqueras, R., Sanchez-Serrano, J.J., and Solano, R. (2003). ETHYLENE RESPONSE FACTOR1 integrates signals from ethylene and jasmonate pathways in plant defense. Plant Cell. 15, 165-178. Ma, B., Yin, C.C., He, S.J., Lu, X., Zhang, W.K., Lu, T.G., Chen, S.Y., and Zhang, J.S. (2014). Ethylene-induced inhibition of root growth requires abscisic acid function in rice (Oryza sativa L.) seedlings. PLoS Genet. 10, pgen 1004701. Ma, B., He, S.J., Duan, K.X., Yin, C.C., Chen, H., Yang, C., Xiong, Q., Song, Q.X., Lu, X., Chen, H.W., Zhang, W.K., Lu, T.G., Chen, S.Y., and Zhang, J.S. (2013). Identification of rice ethylene-response mutants and characterization of MHZ7/OsEIN2 in distinct ethylene response and yield trait regulation. Mol Plant. 6, 1830-1848. Madge Rothenberg, and Ecker, J.R. (1993). Mutant analysis as an experimental approach towards understanding plant hormone action. Seminars in Developmental Biology 4, 3-13. Marina, A., Waldburger, C.D., and Hendrickson, W.A. (2005). Structure of the entire cytoplasmic portion of a sensor histidine-kinase protein. EMBO J. 24, 4247-4259. Mayerhofer, H., Mueller-Dieckmann, C., and Mueller-Dieckmann, J. (2011). Cloning, expression, purification and preliminary X-ray analysis of the protein kinase domain of constitutive triple response 1 (CTR1) from Arabidopsis thaliana. Acta Crystallogr Sect F Struct Biol Cryst Commun 67, 117-120. Mayerhofer, H., Panneerselvam, S., and Mueller-Dieckmann, J. (2012). Protein kinase domain of CTR1 from Arabidopsis thaliana promotes ethylene receptor cross talk. J Mol Biol 415, 768-779. Mayerhofer, H., Panneerselvam, S., Kaljunen, H., Tuukkanen, A., Mertens, H.D., and Mueller-Dieckmann, J. (2014). Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1). J Biol Chem 1, 587667. Merchante, C., Alonso, J.M., and Stepanova, A.N. (2013). Ethylene signaling: simple ligand, complex regulation. Curr Opin Plant Biol. 16, 554-560. Miyazaki, J.H., and Yang, S.F. (1987). The methionine salvage pathway in relation to ethylene and polyamine biosynthesis. Physiologia Plantarum 69, 366-370. Moussatche, P., and Klee, H.J. (2004). Autophosphorylation activity of the Arabidopsis ethylene receptor multigene family. J. Biol. Chem. 279, 48734-48741. Murashige Toshio, and Skoog, F.K. (1962). A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15(3): 473-497. 15, 473-497. Oeller, P.W., Lu, M.W., Taylor, L.P., Pike, D.A., and Theologis, A. (1991). Reversible inhibition of tomato fruit senescence by antisense RNA. Science 254, 437-439. Ohme-Takagi, M., and Shinshi, H. (1995). Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. Plant Cell. 7, 173-182. Ouaked, F., Rozhon, W., Lecourieux, D., and Hirt, H. (2003). A MAPK pathway mediates ethylene signaling in plants. Embo J 22, 1282-1288. Posas, F., Wurgler-Murphy, S.M., Maeda, T., Witten, E.A., Thai, T.C., and Saito, H. (1996). Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 'two-component' osmosensor. Cell. 86, 865-875. Potuschak, T., Lechner, E., Parmentier, Y., Yanagisawa, S., Grava, S., Koncz, C., and Genschik, P. (2003). EIN3-dependent regulation of plant ethylene hormone signaling by two Arabidopsis F box proteins: EBF1 and EBF2. Cell 115, 679-689. Qiao, H., Chang, K.N., Yazaki, J., and Ecker, J.R. (2009). Interplay between ethylene, ETP1/ETP2 F-box proteins, and degradation of EIN2 triggers ethylene responses in Arabidopsis. Genes Dev 23, 512-521. Qiao, H., Shen, Z., Huang, S.S., Schmitz, R.J., Urich, M.A., Briggs, S.P., and Ecker, J.R. (2012). Processing and subcellular trafficking of ER-tethered EIN2 control response to ethylene gas. Science. 338, 390-393. Qu, X., and Schaller, G.E. (2004). Requirement of the histidine kinase domain for signal transduction by the ethylene receptor ETR1. Plant Physiol. 136, 2961-2970. Rechsteiner, M., and Rogers, S.W. (1996). PEST sequences and regulation by proteolysis. Trends Biochem Sci. 21, 267-271. Rodriguez, F.I., Esch, J.J., Hall, A.E., Binder, B.M., Schaller, G.E., and Bleecker, A.B. (1999). A copper cofactor for the ethylene receptor ETR1 from Arabidopsis. Science 283, 996-998. Rogers, S., Wells, R., and Rechsteiner, M. (1986). Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis. Science 234, 364-368. Sakai, H., Hua, J., Chen, Q.G., Chang, C., Medrano, L.J., Bleecker, A.B., and Meyerowitz, E.M. (1998). ETR2 is an ETR1-like gene involved in ethylene signaling in Arabidopsis. Proc Natl Acad Sci U S A 95, 5812-5817. Schaller, G.E., and Bleecker, A.B. (1995). Ethylene-binding sites generated in yeast expressing the Arabidopsis ETR1 gene. Science 270, 1809-1811. Schaller, G.E., and Kieber, J.J. (2002). Ethylene. The Arabidopsis Book 1. Schaller, G.E., Ladd, A.N., Lanahan, M.B., Spanbauer, J.M., and Bleecker, A.B. (1995). The ethylene response mediator ETR1 from Arabidopsis forms a disulfide-linked dimer. J Biol Chem 270, 12526-12530. Schenk, P.M., Kazan, K., Wilson, I., Anderson, J.P., Richmond, T., Somerville, S.C., and Manners, J.M. (2000). Coordinated plant defense responses in Arabidopsis revealed by microarray analysis. Proc Natl Acad Sci U S A 97, 11655-11660. Schultz, J., Milpetz, F., Bork, P., and Ponting, C.P. (1998). SMART, a simple modular architecture research tool: identification of signaling domains. Proc Natl Acad Sci U S A. 95, 5857-5864. Shi, Y., Tian, S., Hou, L., Huang, X., Zhang, X., Guo, H., and Yang, S. (2012). Ethylene signaling negatively regulates freezing tolerance by repressing expression of CBF and type-A ARR genes in Arabidopsis. Plant Cell. 24, 2578-2595. Shi, Y.H., Zhu, S.W., Mao, X.Z., Feng, J.X., Qin, Y.M., Zhang, L., Cheng, J., Wei, L.P., Wang, Z.Y., and Zhu, Y.X. (2006). Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation. Plant Cell. 18, 651-664. Solano, R., Stepanova, A., Chao, Q., and Ecker, J.R. (1998). Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1. Genes Dev 12, 3703-3714. Swarup, R., Perry, P., Hagenbeek, D., Van Der Straeten, D., Beemster, G.T., Sandberg, G., Bhalerao, R., Ljung, K., and Bennett, M.J. (2007). Ethylene upregulates auxin biosynthesis in Arabidopsis seedlings to enhance inhibition of root cell elongation. Plant Cell. 19, 2186-2196. Tanaka, Y., Sano, T., Tamaoki, M., Nakajima, N., Kondo, N., and Hasezawa, S. (2005). Ethylene inhibits abscisic acid-induced stomatal closure in Arabidopsis. Plant Physiol. 138, 2337-2343. Tao, J.J., Cao, Y.R., Chen, H., Wei, W., Li, Q.T., Ma, B., Zhang, W.K., Chen, S.Y., and Zhang, J.S. (2015). Tobacco TCTP interacts with ethylene receptor NTHK1 and enhances plant growth through promotion of cell proliferation. Plant Physiol. 169, 96-114 Tomomori, C., Tanaka, T., Dutta, R., Park, H., Saha, S.K., Zhu, Y., Ishima, R., Liu, D., Tong, K.I., Kurokawa, H., Qian, H., Inouye, M., and Ikura, M. (1999). Solution structure of the homodimeric core domain of Escherichia coli histidine kinase EnvZ. Nat Struct Biol. 6, 729-734. Van Kan, Cozijnsen T, Danhash N, and PJ., D.W. (1995). Induction of tomato stress protein mRNAs by ethephon, 2,6-dichloroisonicotinic acid and salicylate. Plant Mol Biol. 27(6), 1205-1213. Voet-van-Vormizeele, J., and Groth, G. (2008). Ethylene controls autophosphorylation of the histidine kinase domain in ethylene receptor ETR1. Mol Plant 1, 380-387. Wang, F., Cui, X., Sun, Y., and Dong, C.H. (2013a). Ethylene signaling and regulation in plant growth and stress responses. Plant Cell Rep. 32 (7), 1099-1109 Wang, H., Liu, G., Li, C., Powell, A.L., Reid, M.S., Zhang, Z., and Jiang, C.Z. (2013b). Defence responses regulated by jasmonate and delayed senescence caused by ethylene receptor mutation contribute to the tolerance of petunia to Botrytis cinerea. Mol Plant Pathol. 14 (5), 453-469 Wang, W., Hall, A.E., O'Malley, R., and Bleecker, A.B. (2003). Canonical histidine kinase activity of the transmitter domain of the ETR1 ethylene receptor from Arabidopsis is not required for signal transmission. Proc Natl Acad Sci U S A 100, 352-357. Wen, X., Zhang, C., Ji, Y., Zhao, Q., He, W., An, F., Jiang, L., and Guo, H. (2012). Activation of ethylene signaling is mediated by nuclear translocation of the cleaved EIN2 carboxyl terminus. Cell Res. 22, 1613-1616. Wilkinson, J.Q., Lanahan, M.B., Clark, D.G., Bleecker, A.B., Chang, C., Meyerowitz, E.M., and Klee, H.J. (1997). A dominant mutant receptor from Arabidopsis confers ethylene insensitivity in heterologous plants. Nat Biotechnol 15, 444-447. Woeste, K.E., Ye, C., and Kieber, J.J. (1999). Two Arabidopsis mutants that overproduce ethylene are affected in the posttranscriptional regulation of 1-aminocyclopropane-1-carboxylic acid synthase. Plant Physiol. 119, 521-530. Wooten, M.W. (2002). In-gel kinase assay as a method to identify kinase substrates. Sci STKE 2002, pl15. Wuriyanghan, H., Zhang, B., Cao, W.H., Ma, B., Lei, G., Liu, Y.F., Wei, W., Wu, H.J., Chen, L.J., Chen, H.W., Cao, Y.R., He, S.J., Zhang, W.K., Wang, X.J., Chen, S.Y., and Zhang, J.S. (2009). The ethylene receptor ETR2 delays floral transition and affects starch accumulation in rice. Plant Cell. 21 (5), 1473-1494. Xie, C., Zhang, J.S., Zhou, H.L., Li, J., Zhang, Z.G., Wang, D.W., and Chen, S.Y. (2003). Serine/threonine kinase activity in the putative histidine kinase-like ethylene receptor NTHK1 from tobacco. Plant J 33, 385-393. Yang, C., Ma, B., He, S.J., Xiong, Q., Duan, K.X., Yin, C.C., Chen, H., Lu, X., Chen, S.Y., and Zhang, J.S. (2015). MHZ6/OsEIL1 and OsEIL2 regulate ethylene response of roots and coleoptiles and negatively affect salt tolerance in rice. Plant Physiol. pp.15.00353 Yeh, S.H., Lee, B.H., Liao, S.C., Tsai, M.H., Tseng, Y.H., Chang, H.C., Yang, C.C., Jan, H.C., Chiu, Y.C., and Wang, A.Y. (2013). Identification of genes differentially expressed during the growth of Bambusa oldhamii. Plant Physiol Biochem. 63, 217-26. Yin, C.C., Ma, B., Collinge, D.P., Pogson, B.J., He, S.J., Xiong, Q., Duan, K.X., Chen, H., Yang, C., Lu, X., Wang, Y.Q., Zhang, W.K., Chu, C.C., Sun, X.H., Fang, S., Chu, J.F., Lu, T.G., Chen, S.Y., and Zhang, J.S. (2015). Ethylene responses in rice roots and coleoptiles are differentially regulated by a carotenoid isomerase-mediated abscisic acid pathway. Plant Cell. 27, 1061-1081. Yoo, S.D., Cho, Y.H., Tena, G., Xiong, Y., and Sheen, J. (2008). Dual control of nuclear EIN3 by bifurcate MAPK cascades in C2H4 signalling. Nature 451, 789-795. Yu, Y.B., Adams, D.O., and Yang, S.F. (1979). 1-aminocyclopropanecarboxylate synthase, a key enzyme in ethylene biosynthesis. Arch Biochem Biophys. 198, 280-286. Zeng, G. (1998). Sticky-end PCR: new method for subcloning. Biotechniques 25, 206-208. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51772 | - |
dc.description.abstract | 本實驗室由綠竹筍 cDNA 庫中選殖出乙烯受體基因,命名為 BoERS1。其基因全長為 1899 bp。利用 PLACE (plant cis-acting regulatory DNA elements) 分析 BoERS1 5’-端上游序列,發現許多與植物荷爾蒙、光照及缺水逆境相關之 cis-acting elements。BoERS1 可轉譯出含有 632 個胺酸的蛋白質,且序列與水稻及玉米中的乙烯受體相似度達 90%。經由序列分析得知,BoERS1 主要具有三個區塊,分別為:感應功能區塊 (由三個穿膜區組成),GAF 功能區塊及 histidine kinase domain (具有保守性 H、N、G1、F 及 G2 boxes),此區塊分布與原核生物之二元訊息傳遞系統雷同。利用即時聚合酶鏈鎖反應分析 BoERS1 基因表現,田間栽種綠竹筍中 BoERS1 基因表現會隨著莖的生長而增加,且主要分布於節間及生長點,推測 BoERS1 可能參與綠竹快速生長;另外,以植物荷爾蒙處理綠竹瓶苗,發現細胞分裂素及離層酸會使 BoERS1 基因表現下降,而其他植物荷爾蒙較無影響。為瞭解 BoERS1 是否具有 histidine kinase 活性,以酵母菌 Pichia pastoris 表現 BoERS1 之 histidine kinase domain (Ala 331 至 Gly 611, BHK),經質譜儀鑑定重組蛋白質身分後,進行 in vitro 磷酸化實驗,得知 BHK 在 Mn2+ 或 Mg2+ 處理下,具有自我磷酸化活性,而在 Ca2+ 或水處理下則無活性。以質譜儀分析磷酸化反應後之 BHK,發現 Thr 442、Ser 444、Ser 489 及 Ser 503 等胺酸位置有磷酸化現象。將這些胺酸點突變後,再次進行磷酸化實驗,發現點突變並不影響磷酸化活性,說明 BHK 可能呈現多重磷酸化狀態,或具有其它磷酸化位置。以阿拉伯芥乙烯受體 AtETR1 (PDB ID: 4PL9) 及嗜熱菌 (Thermotoga maritima) 之 histidine kinase (PDB ID: 2C2A) 為模版,進行 BHK 結構模擬,發現兩個結構上具有彈性 loops,分別命名為 L1 及 L2。與原核生物比較後發現,L1 為植物 histidine kinase 所獨有,而磷酸化位置 Ser 489 及 Ser 503 正好落在 L1 兩端,推測 BHK 可能利用磷酸化調控 L1 構型,進而改變與其它蛋白質如 CTR1 或 EIN2 之交互作用,進而影響乙烯訊息傳遞;而 L2 正好位於 ATP 結合區上方,可能調控 ATP 能否進入活性區,進一步影響 BHK 磷酸化活性。BoERS1 多重磷酸化的發現,使得我們能夠更進一步地去探討乙烯受體之結構與功能關係。 | zh_TW |
dc.description.abstract | An ethylene receptor gene named BoERS1 was cloned from a bamboo (Bambusa oldhamii) cDNA library. The open reading frame of BoERS1 was 1899 bp which encoded a 632-amino acid polypeptide. The encoded BoERS1 contained three domains, a sensor domain with three transmembrane regions, a GAF domain and a histidine kinase domain that contained all the conserved motifs (H, N, G1, F, and G2) that are present in the histidine kinases of the bacterial two-component systems and shared 90% sequence similarity with other ethylene receptors in plants such as rice or maize. According to real-time PCR analysis, the levels of BoERS1 mRNA in the shoots of field-grown bamboo were elevated along with the growth of the emerging shoots, especially in internodes and shoot meristems. Furthermore, the expression levels of BoERS1 were decreased under benzyladenine (BA, a cytokinin) and ABA treatments in multiple shoots of bamboo. The upstream sequences of BoERS1 were obtained using TAIL-PCR (thermal asymmetric interlaced polymerase chain reaction) and some cis-acting elements related to phytohormones, light, and dehydration were found. With in vitro kinase assay, the recombinant histidine kinase domain of BoERS1 (Ala 331 to Gly 611, BHK) showed autophosphorylation activity in the presence of Mn2+ and Mg2+, but not in the presence of Ca2+ and H2O. LC-ESI-MS/MS analysis indicated that four amino acid residues of BHK, namely Thr 442, Ser 444, Ser 489 and Ser 503, were phosphorylated by an in vitro kinase assay. Site-directed mutagenesis of these amino acids did not affect the phosphorylation activity of BHK. It indicated BHK was multiphosphorylated or had other phosphorylation residues. The model of BHK was built according to the structure of AtETR1 (PDB ID: 4PL9) and a histidine kinase of Thermotoga maritima (PDB ID: 2C2A). The three dimensional model of BHK had two flexible loops, namely L1 and L2. It is interesting to note that Ser 489 and Ser 503 were located in the both ends of L1 which was unique to the plant histidine kinase-containing enzymes and the phosphorylation may regulate the interactions between BoERS1 and other proteins; meanwhile L2 may be a gatekeeper of ATP binding pocket and regulate the entry of ATP. The identification of multiple phosphorylation sites on BoERS1 provides a new avenue for future structure–function studies of the ethylene receptor protein family. | en |
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dc.description.tableofcontents | 目錄 I
縮寫表 V 摘要 VIII ABSTRACT X 第一章 緒論 1 1.1 乙烯簡介 1 1.2 乙烯生合成路徑 2 1.3 乙烯訊息傳遞介紹 3 1.3.1 乙烯受體 4 1.3.1.1 乙烯受體功能區塊介紹 4 1.3.1.2 二元訊息傳遞系統 6 1.3.1.3 磷酸化活性與乙烯訊息傳遞 7 1.3.1.4 乙烯受體結構 8 1.3.2 CTR1 (constitutive triple response 1) 介紹 9 1.3.3 EIN2 (ethylene insensitive 2) 介紹 12 1.3.4 EIN3 (ethylene insensitive 3) 介紹 12 1.3.5 乙烯與其他植物荷爾蒙的交互作用 15 1.4 竹 17 1.4.1 竹的特殊生理現象 17 1.4.2 綠竹筍 18 1.4.3 研究綠竹筍乙烯受體之動機 18 第二章 材料與方法 20 2.1實驗材料 20 2.1.1 菌種 20 2.1.2 載體 20 2.1.3 植物材料 21 2.2實驗藥品 22 2.2.1 一般化學試劑 22 2.2.2 酵素 (限制酶及核酸修飾酶) 22 2.2.3 大腸桿菌用培養基 22 2.2.4 Pichia pastoris用培養基 23 2.3 儀器設備 25 2.3.1 離心機 25 2.3.2 分光光度計 25 2.3.3 核酸電泳設備 25 2.3.4 蛋白質電泳設備 25 2.3.5 其他 25 2.4 實驗方法 26 2.4.1 DNA 抽取與分析 26 2.4.1.1 質體 DNA 之抽取 (Spin-column method) 26 2.4.1.2 Genomic DNA 之抽取 27 2.4.1.3 限制酶切割分析 27 2.4.1.4 Agarose膠體電泳 28 2.4.1.5 DNA 之分離與純化 29 2.4.1.6 DNA 之磷酸化反應 29 2.4.2 基因選殖與保存 30 2.4.2.1 聚合酶鏈鎖反應 (Polymerase Chain Reaction, PCR) 30 2.4.2.2 TAIL (thermal asymmetric interlaced)-PCR 30 2.4.2.3 PCR 反應產物之純化 (PCR clean up) 33 2.4.2.4 T-A cloning 33 2.4.2.5 質體轉形 (transformation) 34 2.4.2.6轉形菌株的篩選 36 2.4.3 以即時聚合酶鏈鎖反應 (real-time PCR) 分析 BoERS1 基因表現 36 2.4.3.1 綠竹筍 RNA 之抽取 36 2.4.3.2 DNase 處理 37 2.4.3.3 反轉錄 (reverse transcription) 37 2.4.3.4 即時聚合酶鏈鎖反應 38 2.4.4 於酵母菌 Pichia pastoris表現重組蛋白質 39 2.4.4.1 Pichia pastoris 勝任細胞之製備 39 2.4.4.2 表現載體之電穿孔轉形 40 2.4.4.3 酵母菌 genomic DNA 之抽取 40 2.4.4.4 Mut+ 表現型酵母菌之 PCR 鑑定 41 2.4.4.5 Pichia pastoris 轉形株 Mut 表現型之鑑定 42 2.4.4.6 最佳誘導時間探討 42 2.4.4.7 重組蛋白質之大量表現 44 2.4.4.8 重組蛋白質之純化 44 2.4.5 蛋白質檢定與活性分析 46 2.4.5.1 蛋白質定量法 46 2.4.5.2 SDS 膠體電泳 46 2.4.5.3 膠片染色法 50 2.4.5.4 膠片乾燥法 50 2.4.5.5 蛋白質電泳轉印法 51 2.4.5.6 免疫呈色法 52 2.4.5.7 蛋白質質譜分析 53 2.4.5.8 Histidine kinase 活性鑑定 55 2.4.5.9 In gel kinase assay 56 2.4.5.10 蛋白質結構模擬 58 第三章 結果與討論 59 3.1 BOERS1 基因相關研究 59 3.1.1 BoERS1 啟動子序列分析 59 3.1.2 BoERS1 於田間栽種綠竹筍中不同生長時期與部位之基因表現 60 3.1.3 BoERS1 於綠竹瓶苗不同植物荷爾蒙處理之基因表現 61 3.2 BOERS1 性質分析 62 3.2.1 蛋白質序列分析 62 3.2.2 BHK 重組蛋白質表現與純化 63 3.2.3 BHK 重組蛋白質磷酸化活性分析 64 3.2.4 以質譜儀分析 BHK 重組蛋白質磷酸化位置 65 3.2.5 BHK 重組蛋白質磷酸化位置之點突變 67 3.2.6 BHK 重組蛋白質磷酸化實驗待改進處 69 3.2.7 蛋白質結構分析 70 第四章 未來展望 73 4.1 BHK 磷酸化胺酸之確認 73 4.2 BHK 及突變株自我磷酸化活性之比較 73 4.3 BHK 磷酸化可能參與之功能 74 4.4 BOERS1 可能之功能 75 參考文獻 76 圖表集 85 附錄 117 問答集 119 | |
dc.language.iso | zh-TW | |
dc.title | 綠竹筍乙烯受體 BoERS1 結構與功能之研究 | zh_TW |
dc.title | Studies on the structure and function of ethylene response sensor 1 (BoERS1) in Bambusa oldhamii | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-1 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 李平篤,王愛玉,李昆達,陳佩燁 | |
dc.subject.keyword | 綠竹,乙烯受體,組胺酸激?,磷酸化, | zh_TW |
dc.subject.keyword | Bambusa oldhamii,ethylene receptor,histidine kinase,phosphorylation, | en |
dc.relation.page | 122 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2015-10-28 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
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