請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51470
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 黃念祖(Nien-Tsu Huang) | |
dc.contributor.author | Chuan-Kai Yang | en |
dc.contributor.author | 楊筌凱 | zh_TW |
dc.date.accessioned | 2021-06-15T13:35:23Z | - |
dc.date.available | 2018-02-16 | |
dc.date.copyright | 2016-02-16 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-01-28 | |
dc.identifier.citation | 1 Clark, L. C. & Lyons, C. ELECTRODE SYSTEMS FOR CONTINUOUS MONITORING IN CARDIOVASCULAR SURGERY. Annals of the New York Academy of Sciences 102, 29-& (1962).
2 Nordling, M., Elmgren, M., Stahlberg, J., Pettersson, G. & Lindquist, S.-E. A Combined Cellobiose OxidaseGlucose Oxidase Biosensor for HPLC Determination On-Line of Glucose and Soluble Cellodextrines. Analytical Biochemistry 214, 389-396 (1993). 3 Martinoia, S. et al. Development of ISFET array-based microsystems for bioelectrochemical measurements of cell populations. Biosensors & Bioelectronics 16, 1043-1050 (2001). 4 Cui, Y., Wei, Q. Q., Park, H. K. & Lieber, C. M. Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species. Science 293, 1289-1292 (2001). 5 Babacan, S., Pivarnik, P., Letcher, S. & Rand, A. G. Evaluation of antibody immobilization methods for piezoelectric biosensor application. Biosensors & Bioelectronics 15, 615-621 (2000). 6 Kenworthy, A. K. Imaging protein-protein interactions using fluorescence resonance energy transfer microscopy. Methods 24, 289-296 (2001). 7 Hoぴoぴk, F. et al. A comparative study of protein adsorption on titanium oxide surfaces using in situ ellipsometry, optical waveguide lightmode spectroscopy, and quartz crystal microbalancedissipation. Colloids and Surfaces B-Biointerfaces 24, 155-170 (2002). 8 Haes, A. J., Chang, L., Klein, W. L. & Duyne, R. P. V. Detection of a Biomarker for Alzheimer’s Disease from Synthetic and Clinical Samples Using a Nanoscale Optical Biosensor. JACS 127, 2264-2271 (2005). 9 Das, A., Zhao, J., Schatz, G. C., Sligar, S. G. & Duyne, R. P. V. Screening of Type I and II Drug Binding to Human Cytochrome P450-3A4 in Nanodiscs by Localized Surface Plasmon Resonance Spectroscopy. Analytical Chemistry 81, 3754-3759 (2009). 10 OHTA, T., ITO, M., KOTANI, T. & HATTORI, T. Emission Enhancement of Laser-Induced Breakdown Spectroscopy by Localized Surface Plasmon Resonance for Analyzing Plant Nutrients. Applied Spectroscopy 63, 555-558 (2009). 11 Lin, T. J., Huang, K. T. & Liu, C. Y. Determination of organophosphorous pesticides by a novel biosensor based on localized surface plasmon resonance. Biosens Bioelectron 22, 513-518 (2006). 12 Lee, T. H. et al. Signal amplification by enzymatic reaction in an immunosensor based on localized surface plasmon resonance (LSPR). Sensors 10, 2045-2053 (2010). 13 林啟萬 et al. 表面電漿子共振生物感測器之最新發展. 化學 69, 211-221 (2011). 14 Sonnichsen, C., Reinhard, B. M., Liphardt, J. & Alivisatos, A. P. A molecular ruler based on plasmon coupling of single gold and silver nanoparticles. Nature biotechnology 23, 741-745 (2005). 15 Li, Y., Lee, H. J. & Corn, R. M. Detection of Protein Biomarkers Using RNA Aptamer Microarrays and Enzymatically Amplified Surface Plasmon Resonance Imaging. Anal. Chem. 79, 1082-1088 (2007). 16 Endo, T. et al. Multiple Label-Free Detection of Antigen-Antibody Reaction Using Localized Surface Plasmon Resonance-Based Core-Shell Structured Nanoparticle Layer Nanochip. Anal. Chem. 78, 6465-6475 (2006). 17 Huang, T., Nallathamby, P. D. & Xu, X.-H. N. Photostable Single-Molecule Nanoparticle Optical Biosensors for Real-Time Sensing of Single Cytokine Molecules and Their Binding reactions. JACS 130, 17095-17105 (2008). 18 Lin, Y.-C. et al. The enhancement method of optical fiber biosensor based on surface plasmon resonance with cold plasma modification. Sensors and Actuators B: Chemical 133, 370-373 (2008). 19 Guo, L. & Kim, D. H. LSPR biomolecular assay with high sensitivity induced by aptamer-antigen-antibody sandwich complex. Biosens Bioelectron 31, 567-570 (2012). 20 Hall, W. P., Ngatia, S. N. & Van Duyne, R. P. LSPR Biosensor Signal Enhancement Using Nanoparticle-Antibody Conjugates. The journal of physical chemistry. C, Nanomaterials and interfaces 115, 1410-1414 (2011). 21 Xie, L., Yan, X. & Du, Y. An aptamer based wall-less LSPR array chip for label-free and high throughput detection of biomolecules. Biosens Bioelectron 53, 58-64 (2014). 22 Oh, B.-R. et al. Integrated Nanoplasmonic Sensing for Cellular Functional Immunoanalysis Using Human Blood. ACSNANO 8, 2667-2676 (2014). 23 Chen, P. et al. Multiplex Serum Cytokine Immunoassay Using Nanoplasmonic Biosensor Microarrays. ACSNANO 9, 4173-4181 (2015). 24 Petryayeva, E. & Krull, U. J. Localized surface plasmon resonance: nanostructures, bioassays and biosensing--a review. Anal Chim Acta 706, 8-24 (2011). 25 Brodoceanu, D. et al. Fabrication of metal nanoparticle arrays by controlled decomposition of polymer particles. Nanotechnology 24, 085304 (2013). 26 Lucas, B. D., Kim, J.-S., Chin, C. & Guo, L. J. Nanoimprint Lithography Based Approach for the Fabrication of Large-Area, Uniformly-Oriented Plasmonic Arrays. Advanced Materials 20, 1129-1134 (2008). 27 Švorčík, V., Kvítek, O., Lyutakov, O., Siegel, J. & Kolská, Z. Annealing of sputtered gold nano-structures. Applied Physics A 102, 747-751 (2010). 28 黃柏瑋. 金及三氧化二釓雙層奈米晶體記憶體之特性研究 碩士論文, 長庚大學, (2012). 29 Hicks, E. M., Zou, S., Schatz, G. C., Spears, K. G. & Duyne, R. P. V. Controlling Plasmon Line Shapes through Diffractive Coupling in Linear Arrays of Cylindrical Nanoparticles Fabricated by Electron Beam Lithography. Nano Letters 5, 1065-1070 (2005). 30 Lin, Y., Zou, Y., Mo, Y., Guo, J. & Lindquist, R. G. E-beam patterned gold nanodot arrays on optical fiber tips for localized surface plasmon resonance biochemical sensing. Sensors 10, 9397-9406 (2010). 31 Bosman, M., Keast, V. J., Watanabe, M., Maaroof, A. I. & Cortie, M. B. Mapping surface plasmons at the nanometre scale with an electron beam. Nanotechnology 18, 165505 (2007). 32 Jensen, T. R., Malinsky, M. D., Haynes, C. L. & Duyne, R. P. V. Nanosphere Lithography Tunable Localized Surface Plasmon Resonance Spectra of Silver Nanoparticles. J. Phys. Chem. B 104, 10549-10556 (2000). 33 Haynes, C. L. & Duyne, R. P. V. Nanosphere Lithography A Versatile Nanofabrication Tool for Studies of Size-DependentNanoparticle Optics. J. Phys. Chem. B 105, 5599-5611 (2001). 34 Liang, C.-C. et al. Plasmonic metallic nanostructures by direct nanoimprinting of gold nanoparticles. OPTICS EXPRESS 19, 4768-4776 (2011). 35 Barbillon, G. Plasmonic Nanostructures Prepared by Soft UV Nanoimprint Lithography and Their Application in Biological Sensing. Micromachines 3, 21-27 (2012). 36 Jia, K., Bijeon, J.-L., Adam, P.-M. & Ionescu, R. E. Large Scale Fabrication of Gold Nano-Structured Substrates Via High Temperature Annealing and Their Direct Use for the LSPR Detection of Atrazine. Plasmonics 8, 143-151 (2012). 37 Fang, S.-U., Hsu, C.-L., Hsu, T.-C., Juang, M.-Y. & Liu, Y.-C. Surface roughness-correlated SERS effect on Au island-deposited substrate. Journal of Electroanalytical Chemistry 741, 127-133 (2015). 38 Švorčík, V. et al. Characterization of evaporated and sputtered thin Au layers on poly(ethylene terephtalate). Journal of Applied Polymer Science 99, 1698-1704 (2006). 39 Henley, S. J., Carey, J. D. & Silva, S. R. P. Pulsed-laser-induced nanoscale island formation in thin metal-on-oxide films. Physical Review B 72 (2005). 40 S. Link, C. B., Mohamed, M. B., B. Nikoobakht & El-Sayed, M. A. Laser Photothermal Melting and Fragmentation of Gold Nanorods Energy and Laser pulse-Width Dependence. J. Phys. Chem. A 103, 1165-1170 (1999). 41 Tseng, S. C. et al. Laser-induced jets of nanoparticles: exploiting air drag forces to select the particle size of nanoparticle arrays. Nanoscale 5, 2421-2428 (2013). 42 Sun, Y. & Xia, Y. Increased Sensitivity of Surface Plasmon Resonance of Gold Nanoshells Compared to That of Gold Solid Colloids in Response to Environmental Changes. Anal. Chem. 74, 5297-5305 (2002). 43 Lee, K.-S. & El-Sayed, M. A. Gold and Silver Nanoparticles in Sensing and Imaging Sensitivity of Plasmon Response to Size, Shape, and Metal Composition. J. Phys. Chem. B 110, 19220-19225 (2006). 44 Miller, M. M. & Lazarides, A. A. Sensitivity of Metal Nanoparticle Surface Plasmon Resonance to the Dielectric Environment. J. Phys. Chem. B 109, 21556-21565 (2005). 45 Kedem, O., Tesler, A. B., Vaskevich, A. & Rubinstein, I. Sensitivity and Optimization of LSPR transducers. ACSNANO 5, 748-760 (2011). 46 邱國斌 & 蔡定平. 金屬表面電漿簡介. 物理雙月刊 28, 472-485 (2006). 47 Willets, K. A. & Van Duyne, R. P. Localized surface plasmon resonance spectroscopy and sensing. Annual review of physical chemistry 58, 267-297 (2007). 48 李舒昇. 以拋物面鏡為基礎之表面電漿共振儀研究生物晶片上分子反應介面之親和力 博士論文, 國立臺灣大學, (2004). 49 楊沛東. 高解析掃描式表面電漿共振顯微鏡應用於大腸癌腫瘤標誌陣列晶片之檢測 碩士論文, 國立臺灣大學, (2014). 50 曾賢德. 金奈米粒子的表面電漿共振特性. 物理雙月刊 32, 126-135 (2010). 51 楊佳慶. 利用聚焦離子束製作AlGaN/GaN奈米線之金氧半場效應電晶體 碩士論文, 國立中山大學, (2008). 52 彭偉倫. 氧化銦錫透明電極應用於氮化鎵發光二極體 碩士論文, 國立交通大學, (2003). 53 Homola, J. ı., Yee, S. S. & Gauglitz, G. Surface plasmon resonance sensors revie. Sensors and Actuators 54, 3-15 (1999). 54 http://ibidi.com/xtproducts/en/ibidi-Labware/sticky-Slides/sticky-Slide-VI-0.4, 2016). 55 Rakic´, A. D., ic´, A. B. D., Elazar, J. M. & Majewski, M. L. Optical properties of metallic films for vertical-cavity optoelectronic devices. APPLIED OPTICS 37, 5271-5283 (1998). 56 Lopatynskyi, A. M. et al. Au nanostructure arrays for plasmonic applications: annealed island films versus nanoimprint lithography. Nanoscale research letters 10, 99 (2015). 57 Ozhikandathil, J. & Packirisamy, M. Simulation and implementation of a morphology-tuned gold nano-islands integrated plasmonic sensor. Sensors 14, 10497-10513 (2014). 58 Zakai, N. A. et al. A Prospective Study of Anemia Status, Hemoglobin Concentration, and Mortality in an Elderly Cohort. ARCH INTERN MED 165, 2214-2220 (2005). 59 Pai, M., Riley, L. W. & Colford, J. M. Interferon-γ assays in the immunodiagnosis of tuberculosis: a systematic review. The Lancet Infectious Diseases 4, 761-776 (2004). 60 Lange, C. & Mori, T. Advances in the diagnosis of tuberculosis. Respirology 15, 220-240 (2010). 61 Eddings, M. A., Johnson, M. A. & Gale, B. K. Determining the optimal PDMS–PDMS bonding technique for microfluidic devices. Journal of Micromechanics and Microengineering 18, 067001 (2008). | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51470 | - |
dc.description.abstract | 表面電漿共振是近年來在奈米光學領域內相當熱門的研究課題,因其具有免標定、非侵入式且高靈敏度等優點,常被應用在化學或生物感測器上,而侷域型表面電漿共振因為光學架構設計較為簡易且適用於即時性的量測,其相關研究獲得了廣泛的關注。本論文以檢驗抗體抗原的專一性鍵結為出發點,開發了一製程簡單且快速,且可大面積製作(2cm x 2cm)的侷域型表面電漿共振奈米金膜生物感測器,使用電子束蒸鍍以及快速熱退火來製作隨機分布的金奈米粒子結構,藉由調整三種製程參數:(1)鍍膜厚度;(2)退火溫度及(3)退火時間來做出不同尺寸與間距的金奈米粒子結構,並利用UV-VIS光譜儀以及原子力顯微鏡來量測奈米金膜的吸收光譜和表面結構。從量測結果中得出6-10nm的奈米金膜經過5分鐘900°C下的熱退火後會有較尖銳的吸收波峰,再從不同濃度的葡萄糖溶液實驗中得知10nm退火後的奈米金膜有最高的折射率靈敏度161.5 nm/RIU,且有較為一致的表面結構與吸收光譜(波峰位置差距< 1%)。為了更進一步研究隨機形狀與排列之金奈米粒子結構所產生的侷域型表面電漿共振電磁場和吸收光譜,本研究以原子力顯微鏡所得到的量測結果為基準,在有限元素分析軟體COMSOL上建立了一個等效的金奈米粒子陣列模型,來模擬並以數值方式計算經過不同條件的退火後,金奈米粒子的尺寸改變對吸收光譜造成的影響,未來可藉此微調退火參數。最後,本研究整合此侷域型表面電漿共振感測晶片和一商用的微流道晶片,達成了一個微型化的免疫分析系統,進行動態且連續性的多重細胞因子檢測,並維持良好的專一性與靈敏度且減少樣本需求(45μl),未來若能再將光學架構微縮並整合,將可實現定點照護的理念。 | zh_TW |
dc.description.abstract | Surface plasmon resonance is a hot research topic in nanooptics field recently. Due to its label-free, non-invasive and highly sensitive merits, localized surface plasmon resonance based sensing techniques have been widely utilized in chemical or biosensing applications with simplified optical settings and real-time detection capability. In this paper, we developed a simple and fast nanostructure fabrication method using electron beam evaporation deposition and rapid thermal annealing (RTA) treatment to fabricate a large-area (2cm x 2cm) biosensor utilizing localized surface plasmon resonance (LSPR) nanoplasmonic effects. To get the best LSPR sensing performance of the gold nanostructure, we adjusted three fabrication conditions: (1) deposition thickness; (2) annealing temperature and (3) the annealing time. We then observed the absorbance spectrum profile and surface morphology using the UV-VIS spectrometer and atomic force microscopy (AFM). Based on the results, we discovered that the fabrication conditions at 6-10nm gold deposition under 900°C RTA treatment for 5 minutes shows sharper and stronger absorbance spectrum. We then test the sensitivity of these sensor by using glucose-water solution of different concentration. We found out that the 10nm gold chip shows the highest sensitivity 161.5 nm/RIU and holds a fine uniformity (peak wavelength variation < 1%). To study the electromagnetic field and the absorbance spectrum of the arbitrary-shaped and random-distributed nanoparticles fabricated by RTA treatment, we constructed an effective nanostructure array based on AFM scanned results and used a finite-element method (FEM) software COMSOL to numerically analyze the dependence of absorbance spectrum on the different height of nanoparticles for future alternation of annealing parameters. Finally, we integrated this LSPR sensor with a commercial microfluidic channel as an immunoanalysis platform to achieve dynamic and continuous detection of multiple cytokines with reduced sample volume (45μl) while still demonstrating fine specificity and sensitivity. In the future, if we can minimize the optical structure and integrates it into the system, we will be able to realize the point-of-care testing approach. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T13:35:23Z (GMT). No. of bitstreams: 1 ntu-105-R02945015-1.pdf: 5950889 bytes, checksum: 08b81227ed76180069025655428be625 (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 口試委員會審定書 #
誌謝 i 中文摘要 iii ABSTRACT iv 目錄 vi 圖目錄 ix 表目錄 ix Chapter 1 緒論 1 1.1 研究背景與研究動機 1 1.2 文獻回顧 3 1.2.1 生物感測器 3 1.2.2 侷域表面電漿共振感測器之應用 6 1.2.3 侷域表面電漿共振奈米金膜製程技術 12 1.3 論文架構 16 Chapter 2 表面電漿共振光學原理 17 2.1 金屬物質內部的光學特性 17 2.2 表面電漿共振原理 20 2.3 侷域型表面電漿共振量測原理 24 2.4 快速熱退火基本原理 27 Chapter 3 研究方法與步驟 28 3.1 系統架構研發與設計理念 28 3.2 LSPR感測晶片製程 31 3.3 多重生物分子量測實驗 35 3.4 免疫細胞捕獲微流道設計與製程 37 Chapter 4 有限元素分析法模擬 40 4.1 模擬模型與架構 40 4.1.1 Drude-Lorentz電漿模型 40 4.1.2 金奈米粒子模擬架構設計 41 4.2 模擬結果 43 4.2.1 金奈米粒子尺寸模擬結果 43 4.2.2 金奈米粒子折射率靈敏度模擬結果 46 4.2.3 金奈米粒子結構之連續性模擬結果 47 Chapter 5 實驗結果與討論 50 5.1 LSPR感測晶片之光譜分析與快速熱退火製程參數選擇 50 5.1.1 金膜光學性質分析 50 5.1.2 金膜物理性質分析 54 5.1.3 金膜電性分析 58 5.1.4 LSPR金膜之重複性測試 59 5.1.5 LSPR金膜之靈敏度測試 61 5.2 生物分子量測實驗結果 63 5.2.1 表面SAM鍵結均勻度測試 63 5.2.2 血紅蛋白量測結果 65 5.2.3 IFN-γ量測結果 67 5.2.4 TNF-α量測結果 70 5.2.5 免疫細胞捕獲微流道製作結果 71 Chapter 6 結論與未來展望 73 6.1 結論 73 6.2 未來展望 75 參考文獻 77 | |
dc.language.iso | zh-TW | |
dc.title | 使用快速熱退火製作之侷域型表面電漿共振感測器進行多重生物標記物檢測 | zh_TW |
dc.title | Using Rapid Thermal Annealing Fabricated Localized Surface Plasmon Resonance Sensor for Multiple Biomarkers Detection | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林致廷(Chih-Ting Lin),王倫(Lon Wang),黃榮山(Long-Sun Huang),許聿翔(Yu-Hsiang Hsu) | |
dc.subject.keyword | 表面電漿共振,侷域型表面電漿共振,快速熱退火,生物感測器,生物標記物,細胞因子, | zh_TW |
dc.subject.keyword | Surface Plasmon Resonance,Localized Surface Plasmon Resonance,Rapid Thermal Annealing,Biosensor,Biomarker,Cytokine, | en |
dc.relation.page | 80 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-01-28 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
顯示於系所單位: | 生醫電子與資訊學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-105-1.pdf 目前未授權公開取用 | 5.81 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。