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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51462完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張建成(Chien-Cheng Chang) | |
| dc.contributor.author | Yu-Huan Lin | en |
| dc.contributor.author | 林育環 | zh_TW |
| dc.date.accessioned | 2021-06-15T13:35:06Z | - |
| dc.date.available | 2021-02-15 | |
| dc.date.copyright | 2016-02-15 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-01-29 | |
| dc.identifier.citation | [1]Haeberle, Stefan, and Roland Zengerle. 'Microfluidic platforms for lab-on-a-chip applications.' Lab on a Chip 7.9 (2007): 1094-1110.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51462 | - |
| dc.description.abstract | 儘管現今疾病醫療如此進步，藥物的篩檢仍是一費時且漫長的工程，為了提升心臟毒性藥物篩檢之效率，開發一種以壓電為基礎之體外仿生心臟晶片為本研究之主要構想，利用壓電的力電轉換特性電特性，做為即時檢測晶片之優勢，將可應用於動物臨床測試中之快速藥物篩檢，以大幅提升篩檢速率。本研究以壓電高分子聚合物(PVDF、P(VDF-TrFE))之柔性壓電材料做為此晶片薄膜研發之材料，以三維立體結構之微懸臂樑做為其薄膜之結構開發設計，並依心肌細胞之尺度及培養環境需求，研發出低成本壓電薄膜之微機電製程技術。
此外，隨著疾病預防觀念之普及，各類型之檢測需求及其檢測之精準度大幅增加。近年來研究發現，適體(Aptamer)可與大部份分子形成專一性結合之特性、並具有相當之穩定性、以及低成本之可大量製造之優勢，足以做為分子檢測之應用。本研究利用膠體電泳之電泳遷移率分析(EMSA)證實其適體做為分子檢測方法之可行性，且透過自製晶片之設計與機電整合技術研發一微電泳膠體系統，配合即時定量聚合酶鏈鎖反應(Quantitative real time polymerase chain reaction， qPCR)，進一步證實其自製分子檢測晶片之微電泳膠體系統以達到可定性分子之晶片檢測能力，在某程度上具備有一個次級之定量解析能力，顯示其適體檢測方法與晶片未來之可定量分析潛能。 | zh_TW |
| dc.description.abstract | Despite the advances in medical diseases today, drugs screening is still a time-consuming and lengthy process. In order to enhance the efficiency of drug screening for cardiotoxin detection, developing a biomimetic heart on a chip based on piezoelectricity is the major goal of this study. The advantage of using piezoelectric material is its electro-mechanical coupling effect that can provide a real-time signal for monitoring cardiac activities. Furthermore, having an automatic in vitro biomimetic heart on chip system could potentially enhance the drugs screening process during the drug development. In this study, a piezoelectric polymer (PVDF or P(VDF-TrFE)) is used to serve as the flexible structure of the chip. To fit the culture of a single or several myocardial cells, a low cost microfabrication process of a piezoelectric thin-film was developed in this study. Developed process and experimental results will be presented in this thesis.
On the other hand, a new methods based-on Aptamer for molecules detection is developed in this srudy. It provides excellent specificity for a large populations of molecules. Further, the production of aptamer is low-cost and stable. Researches have shown that aptamer is a good candidate for future molecular detection methods. In this study, we apply electrophoretic mobility electrophoresis analysis (EMSA) to demonstrate the feasibility of using aptamer for molecular detection. A miniature electrophoresis device was developed for verification of this concept. The real-time quantitative polymerase chain reaction (qPCR) is used to analyze the quantity of detected molecules. It was confirmed that using aptamer to detect molecular is feasible and could potentially provide quantified information. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T13:35:06Z (GMT). No. of bitstreams: 1 ntu-105-R02543020-1.pdf: 9156335 bytes, checksum: 663dee839afe4edd9c5d9b1dcd148ad0 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 致謝 I
中文摘要 II ABSTRACT III 目錄 IV 圖目錄 VII 表目錄 XIII 第一章 緒論 1 1.1 前言 1 1.2 研究動機 2 1.2.1 仿生心臟晶片之壓電系統製程開發 2 1.2.2 DNA適體分子檢測晶片之研發 3 1.3 文獻回顧 4 1.3.1 仿生心臟晶片之發展 4 1.3.2 壓電材料現今應用及技術 9 1.3.3 分子檢測的技術的發展 13 1.4 研究方法 17 1.4.1 仿生心臟晶片之壓電系統製程開發 17 1.4.2 DNA適體分子檢測晶片之研發 17 1.5 論文架構 17 第二章 仿生心臟晶片之設計理念 19 2.1 心肌細胞的種類、特徵與特性介紹 19 2.2 微奈米結構設計與製程介紹 20 2.3 壓電系統材料與特性 24 2.3.1 壓電基本性質與壓電效應 24 2.3.2 壓電材料種類與性質比較 26 2.3.3 壓電之高分子聚合物 27 2.3.4 壓電聚合物之製程方法與限制 30 第三章 壓電薄膜製程開發 34 3.1 結構製程設計與材料選擇 34 3.1.1 光罩設計與尺寸 35 3.1.2 製程開發_已極化之9μm PVDF壓電薄膜 36 3.1.3 製程開發_聚氟乙烯 - 三氟乙烯共聚物P（VDF-TrFE）薄膜 39 3.2 光刻製程(PHOTOLITHOGRAPHY) 44 3.2.1 光刻技術步驟 44 3.2.2 正負光阻及圖案定義 46 3.2.3 曝光對準設備 48 3.2.4 低溫黃光製程研發 48 3.3 金屬蒸鍍及掀舉(LIFT-OFF) 50 3.3.1 電子束蒸鍍(E-beam)之鈦金屬薄膜表面探討 50 3.3.2 Lift-off 54 3.4 蝕刻(ETCHING) 57 3.4.1 鎳銅電極蝕刻 58 3.4.2 PVDF蝕刻 59 第四章 仿生壓電薄膜製程最佳化設計 62 4.1 自製 P(VDF-TRFE)製程總流程 62 4.2 光罩設計與尺寸 64 4.3 SU-8光阻層 65 4.4 改進之濕蝕刻製程 68 4.5 空氣電離子槍(AIR JET)之應用 70 第五章 DNA適體分子檢測晶片之研發設計理念 76 5.1 晶片之設計理念 76 5.1.1 DNA適體之分子檢測方法 76 5.1.2 結核菌感染檢測之晶片研發 78 5.1.3 適體設計與IFN-γ結合 79 5.2 膠體電泳介紹 80 5.2.1 原理與特性 80 5.2.2 電泳遷移率變動分析之應用 82 5.2.3 電泳結果之分析方法 82 5.3 即時定量聚合酶鏈鎖反應(QUANTITATIVE REAL TIME POLYMERASE CHAIN REACTION， QPCR) 83 5.3.1 PCR發展與應用 83 5.3.2 PCR基本原理流程與種類 84 5.3.3 qPCR原理 86 5.3.4 qPCR之絕對定量曲線分析 90 5.4 適體晶片系統架構設計與檢測開發 91 第六章 DNA適體分子檢測應證 93 6.1 實驗設備與材料配置參數 93 6.2 實驗步驟 95 6.3 結果與分析(理論證實) 97 6.3.1 適體(Aptamer)與蛋白(IfN-γ)之監測方法 97 6.3.2 螢光標定之電泳遷移率變動分析結果應證 98 6.3.3 最佳化結果分析與應用 100 第七章 自製ＤＮＡ適體晶片開發 103 7.1 適體晶片材料與系統設計 103 7.1.1 總裝置設計 103 7.1.2 晶片裝置製造與加工 104 7.1.3 實驗設計 105 7.2 適體晶片系統之研發 107 7.2.1 芯片流道設計改良 107 7.2.2 膠體流道製作方法演進 109 7.2.1 111 7.2.2 111 7.2.3 晶片電極材料更換與電壓控制 111 7.2.4 改良之實驗設計 112 7.3 電泳遷移率之結果分析 112 7.3.1 單一電泳分析結果 112 7.3.2 平行電泳分析結果 114 7.4 QPCR結果與定量濃度分析 115 第八章 結論 120 8.1 仿生心臟晶片之壓電系統製程開發 120 8.2 DNA適體分子檢測晶片之研發 120 第九章 參考文獻 121 附錄 127 | |
| dc.language.iso | zh-TW | |
| dc.title | 仿生心臟晶片之壓電系統製程開發及適體分子檢測晶片之研發 | zh_TW |
| dc.title | Development of a piezoelectric microsystem for biomimetic heart on a chip and an aptamer-based gel electrophoresis chip for molecular detection | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 許聿翔(Yu-Hsiang Hsu) | |
| dc.contributor.oralexamcommittee | 周涵怡(Han-Yi, Chou),陳奕帆(Yih-Fan Chen),張家歐(Chia-Ou Chang) | |
| dc.subject.keyword | 壓電高分子聚合物,PVDF,P(VDF-TrFE),適體,電泳遷移率分析, | zh_TW |
| dc.subject.keyword | PVDF,P(VDF-TrFE),Aptamer,EMSA,qPCR, | en |
| dc.relation.page | 127 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-01-29 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 應用力學研究所 | zh_TW |
| 顯示於系所單位： | 應用力學研究所 | |
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