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標題: | 利用榖胱甘肽輔助蛋白質金奈米團簇之合成 Glutathione assisted preparation of gold nanoclusters using minimum amount of protein |
作者: | Bo-Yi Wu 吳柏宜 |
指導教授: | 張煥宗(Huan-Tsung Chang) |
關鍵字: | 金奈米團簇,光致發光,牛血清蛋白,穀胱甘?,亞硝酸鹽, Au nanoclusters,Photoluminescence,BSA,GSH,Nitrite, |
出版年 : | 2016 |
學位: | 碩士 |
摘要: | 本研究藉由使用穀胱甘肽和牛血清白蛋白以合成具有光致發光性質之金奈米團簇(BSA/GSH-Au NCs)。其激發波長與放光波長分別為330 nm與650 nm。在BSA/GSH-Au NCs的製備中,BSA與GSH分別為包覆劑和還原劑。藉由加入穀胱甘肽,只需30 μM BSA即可製備出穩定且具有較強的光致發光性質的BSA/GSH-Au NCs。此方法除了只需30分鐘外,同時可節省昂貴蛋白質試劑的用量。另外,我們同時也成功的將此方法搭配不同蛋白質,如Lysozyme、Ovalbumin、Transferrin,製備出不同的protein/GSH-Au NCs。其光致發光的強度隨著蛋白質所含酪氨酸/半胱氨酸(tyrosine/cysteine)的數目與大小的增加而上升。我們同時也將此材料應用於亞硝酸鹽濃度之偵測。在酸性條件(pH = 3)下,由於NO+為缺電子,會和BSA/GSH-Au NCs作用並氧化BSA/GSH-Au NCs中的金,造成其光致發光強度下降。在最佳化的條件下,此系統之偵測極限為0.3 μM,且具有好的選擇性、高靈敏度及極佳的耐鹽性(在500 mM NaCl溶液中仍有光致發光之特性)。我們也將此系統成功應用於湖水樣品中亞硝酸鹽濃度之偵測,證明此系統於真實樣品中亞硝酸鹽濃度偵測之可行性。 Bovine serum albumin (BSA) and glutathione (GSH) have been used to prepare gold nanoclusters (BSA/GSH-Au NCs) with excitation/emission wavelengths of 330/650 nm from Au3+ at 70 °C for 30 min. The major roles of BSA and GSH in the preparation of BSA/GSH-Au NCs are acting as capping and reducing agents, respectively. With the assistance of GSH, only 30 μM BSA is required to prepare stable and bright BSA/GSH-Au NCs, leading to low cost and ease in configuration of BSA/GSH-Au NCs. In addition to BSA, lysozyme, ovalbumin, and transferrin have also been used to prepare protein/GSH-Au NCs. The decreased order of the photoluminescence intensity of protein/GSH-Au NCs agrees with the decreased orders of size and number of tyrosine/cysteine of the proteins. The photoluminescence of BSA/GSH-Au NCs is quenched by nitrite (NO2-) at pH 3.0, mainly due to the oxidation of BSA/GSH-Au NCs with nitrosyl ions (NO+). This approach allows detection of NO2ˉ down to 0.3 μM, with great selectivity and stability against salt (up to 500 mM NaCl). Practicality of this simple and sensitive approach has been validated by the analysis of pond water samples. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50880 |
DOI: | 10.6342/NTU201600668 |
全文授權: | 有償授權 |
顯示於系所單位: | 化學系 |
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