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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50634完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林?輝(Feng-Huei Lin) | |
| dc.contributor.author | Chen-Yin Su | en |
| dc.contributor.author | 蘇宸瑩 | zh_TW |
| dc.date.accessioned | 2021-06-15T12:49:52Z | - |
| dc.date.available | 2021-08-23 | |
| dc.date.copyright | 2016-08-23 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-07-20 | |
| dc.identifier.citation | 1. C.S. Young, S. Terada, J.P. Vacanti, M. Honda, J.D Bartlett, and P.C. Yelick, Tissue Engineering of Complex Tooth Structures on Biodegradable Polymer Scaffolds. J Dent Res, 2002(81): p.695-700.
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Guo-hua Li, Li-na Niu et al., Ability of new obturation materials to improve the seal of the root canal system: A review. Acta Biomaterialia, 2013(11): p. 14-27. 10. Gulabivala K, Leung SF, Review of a new root canal obturation technique. Dent Update, 1994. 21: p. 73-83. 11. Combe EC, Cohen BD, Cummings K, Alpha- and beta-forms of gutta-percha in products for root canal filling. Int Endod J, 2001. 34: p. 447-451. 12. Siqueira, J.F. and H.P. Lopes, Mechanisms of antimicrobial activity of calcium hydroxide: a critical review. International Endodontic Journal, 1999. 32(5): p. 361-369. 13. Hauman, C.H.J. and R.M. Love, Biocompatibility of dental materials used in contemporary endodontic therapy: a review. Part 1. Intracanal drugs and substances. International Endodontic Journal, 2003. 36(2): p. 75-85. 14. Estrela, C., et al., In vitro determination of direct antimicrobial effect of calcium hydroxide. Journal of Endodontics, 1998. 24(1): p. 15-17. 15. Andreasen, J.O., B. Farik, and E.C. Munksgaard, Long-term calcium hydroxide as a root canal dressing may increase risk of root fracture. Dental Traumatology, 2002. 18(3): p. 134-137. 16. Ronald Ordinola-Zapata, Cl_ovis Monteiro Bramante, Marcelo Haas Villas-Boas, Bruno Cavalini Cavenago, Marco H_ungaro Duarte, Marco Aur_elio Versiani, Morphologic Micro–Computed Tomography Analysis of Mandibular Premolars with Three Root Canals, J. Endod, 2013.39(9):p.1130-1135. 17. Kim S, P.G., Rubinstein R, Dorscher-Kim J, Color Atlas of Microsurgery in Endodontics. 2001. 18. Ricucci, D. and J.F. Siqueira, Jr., Fate of the tissue in lateral canals and apical ramifications in response to pathologic conditions and treatment procedures. J Endod, 2010. 36(1): p. 1-15. 19. Verma, P. and R.M. Love, A Micro CT study of the mesiobuccal root canal morphology of the maxillary first molar tooth. Int Endod J, 2011. 44(3): p. 210-217. 20. Cunningham, C.J. and E.S. Senia, A three-dimensional study of canal curvatures in the mesial roots of mandibular molars. Journal of Endodontics, 1992. 18(6): p. 294-300. 21. Vieira, A.R., et al., Dentinal tubule infection as the cause of recurrent disease and late endodontic treatment failure: a case report. J Endod, 2012. 38(2): p. 250-254. 22. C.P. Lin, F.H Lin, Treatment of tooth fracture by medium energy CO2 laser and DP-bioactive glass paste: compositional, structural, and phase changes of DP-bioglass paste after irradiation by CO2 laser. Biomaterials 2000. 21: p. 637-643. 23. Lan, W.H., et al., Morphologic study of Nd:YAG laser usage in treatment of dentinal hypersensitivity. J Endod, 2004. 30(3): p. 131-134. 24. Lee, B.S., et al., In vitro study of DP-bioglass paste for treatment of dentin hypersensitivity. Dent Mater J, 2005. 24(4): p. 562-9. 25. Lee, B.S., et al., In vitro study of dentinal tubule occlusion with sol-gel DP-bioglass for treatment of dentin hypersensitivity. Dent Mater J, 2007. 26(1): p. 52-61. 26. P. W. Mcmillan, Glass ceramics, 2nd edition, Academic Press, Inc.,London,1979: p.1-7. 27. W. Vogel, Glass chemistry, 2nd edition, Springer-Verlag, Inc., London, 1994:p.1-19. 28. B. E. Warren, Kristallogr. Mineralog. Petrogr., 1933(86):p. 349. 29. W. H. Zachariasen, The atomic arrangement in glass, J. Am. Chem. Soc., 1932(54):p.3841. 30. W. D. Kingery, H. K. Bowen and D. R. Uhlmann, Introduction to Ceramics, 98, Wiley and Sons, 1976. 31. L.L. Hench, The story of Bioglass. J Mater Sci Mater Med, 2006. 17(11): p. 967-978. 32. L. L. Hench, H. A. Paschall, Direct chemical bond of bioactive glass-ceramic materials to bone and muscle. J. Biomed. Mater. Res., 1973(4): p. 27-45. 33. D. Bellucci, V. Cannillo, A. Sola, An Overview of The Effects of Thermal Processing on Bioactive Glasses, Science of Sintering, 2010(42):p. 307-320. 34. Chun Wei Chang, Feng Huei Lin, Wan Hong Lan, Chun Pin Lin, Treatment of dentin hypersensitivity with a bioactive glass. Formosan J Med, 2003(7): p. 42-47. 35. Kodjo Boady Djagny, Zhang Wang, Shiying Xu, Gelatin: A Valuable Protein for Food and Pharmaceutical Industries: Review, Critical Reviews in Food Science and Nutrition, 2001(41):p. 481-492. 36. S.B. Lee, Bio-artificial skin composed of gelatin and (1-3),(1-6)-beta-glucan., Biomaterials, 2003(24): p. 2503-2511. 37. C.T.Hanks, John C.Wataha, Zhilin Sun,In vitro models of biocompatibility: A review. Dent Mater, 1996(12): p. 186-l93. 38. Roche. Cell proliferation reagent WST-1 protocol, 2011. 39. Promega. Cytotoxicity assay protocol, 2006. 40. Ernest M. Levin, et al, Phase diagrams for ceramists, Columbus, Ohio : American Ceramic Society, 1964, v2 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50634 | - |
| dc.description.abstract | 所謂根管治療，是將根管內受感染的牙髓組織移除，最後再使用根管封填材料放入牙根空腔使其密封，阻斷微生物再次經根管系統造成組織感染。然而根管系統的複雜程度超乎想像，相同的牙齒中也存在極大的變異性，彎曲的根管在臨床的治療也是一大挑戰，殘存的微生物便會造成持續的症狀，導致未來根管治療的失敗。目前市面尚無任何材料可以深入牙本質小管中，抑制細菌增長同時封閉牙本質小管。根據本團隊先前的研究，DP-生醫玻璃與30%磷酸調拌後，能在牙本質小管中形成結晶，有效的封閉牙本質小管。
本研究目的為開發一個新鈣磷硼系玻璃，並製作玻璃製劑使其性質產生pH值4左右的酸性環境，能夠釋放鈣離子與磷酸根離子形成再結晶沉澱，有效封閉牙本質小管。本研究第一部分為材料製備，透過高溫熔融及研磨的過程得到微米級玻璃粉末。第二部分為材料分析，目的確認玻璃系統的性質。第三部分為牙本質模型實驗，使用臨床上拔除之人類恆牙來模擬真實牙齒環境，切取牙本質試片測試玻璃製劑是否達到預期牙本質小管封閉。最後一部分進行生物相容性測試，為牙科材料上市前做基本的細胞活性與毒性試驗。 總結實驗結果，本研究以高溫熔融製程可合成出具有高P2O5含量的鈣磷硼系玻璃，BCP-G製劑可在1小時內產生pH值2以下的酸性環境，在人類牙本質模型塗抹測試中，與氫氧化鈣混合的BCP-G-0.1CH製劑可以在24小時內能有效的進入且封閉牙本質小管，並且利用材料萃取液測試結果有良好生物相容性，因此，BCP-G-0.1CH製劑在未來可針對根管治療提供更完善的對策並提高根管治療的成功率。 | zh_TW |
| dc.description.abstract | The main objective of root canal therapy is to through mechanical and chemical cleaning of the entire pulp cavity and completes the occlusion with filling materials. It is obvious that the result of root canal therapy depends on their reduction or elimination of the microorganisms for lesions. However, total elimination of bacteria is difficult to accomplish. The complexity of the root canal system is the main challenge for any dentist undertaking root canal treatment. Nowadays, there is no commercial products can effectively seal dentin tubules. According to previous study in our laboratory, DP-bioglass has demonstrated 60μm of sealing depth in dentin tubules.
The purpose of this study is to develop a CaO-B2O3-P2O5 glass system (BCP-glass) which can release more calcium ion and phosphate ion in order to seal dentin tubules. Furthermore, the glass system will be mixed with gelatin solution. Firstly, reaching the eutectic point through high temperature melting process, the BCP-glass powder is generated and mixed with 3% gelatin solution(G) and calcium hydroxide(CH) to mimic paste-like formation. Then, using the BCP-G-0.1CH paste on the human dentin disc model to observe the sealing ability. Finally, the biocompatibility of BCP-G-0.1CH paste is investigated. The results showed that BCP-glass powder with high P2O5 had been made successfully. The group of BCP-G-0.1CH paste demonstrated 70 μm of sealing depth in dentin tubules in 24 hours. Moreover, BCP-G-0.1CH paste also showed good biocompatibility in WST-1 and LDH assay. In conclusion, BCP-G-0.1CH paste has a potential of dentin tubules occlusion and provides a novel method in root canal therapy. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T12:49:52Z (GMT). No. of bitstreams: 1 ntu-105-R02548045-1.pdf: 3848010 bytes, checksum: 519a7d8e28d26db00cc21952084ffc10 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 第一章 緒論 ................................ ......................... 1
1.1 根管治療的目的................................ ................ 1 1.2 現今根管封填材料與其優缺點 .................................2 1.2.1 鈣矽水泥 ................................. .............. 2 1.2.2 馬來膠 .................................... ................ 3 1.2.3 氫氧化鈣 .................................... .............. 3 1.3 根管治療的難處與微生物感染問題 ...........................4 1.4 本研究團隊針對牙質小管封閉之相關研究.................... 6 1.5 研究目的 ................................. .................... 7 第二章 理論基礎 ................................ ..................... 8 2.1 玻璃之形成與結構 ................................ ............ 8 2.1.1 玻璃之形成 ................................ ............ 8 2.1.2 玻璃的結構 ................................ ............ 9 2.2 生醫玻璃原理與應用 ................................ ......... 10 2.3 明膠性質 ................................ ................... 11 2.4 評估牙科材料生物相容性 ................................ ..... 12 2.4.1 WST-1 細胞活性測試 ................................ ... 12 2.4.2 LDH 細胞毒性測試 ................................ ..... 13 第三章 材料與方法 ................................ .................. 14 3.1 實驗儀器 ................................ ................... 14 3.2 實驗藥品 ................................ ................... 15 3.3 實驗架構 ................................ ................... 16 3.4 鈣磷硼系玻璃的製作與定性分析 ............................... 17 3.4.1 BCP-玻璃的製備 ................................ ....... 17 3.4.2 X 光繞射分 析 (X -Ray diffraction, XRD) ................ 18 3.4.3 穿透式電子顯微鏡 (Transmission Electron Microscopy, TEM) ................................ ....................... 18 3.4.4 能量散射光譜分析儀 (Energy -dispersive X -ray spectroscopy, EDS) ................................ ......... 18 3.5 BCP-玻璃 /明膠製劑的作與分析 .............................. 19 3.5.1 明膠濃度之選擇 ..................................... ....... 19 3.5.2 BCP-玻璃 /明膠製劑之備 .............................. 19 3.5.3 BCP-玻璃 /明膠製劑酸鹼值變化 .......................... 19 3.6 人類牙本質模型 .................................... ............ 20 3.6.1 牙本質樣品之製備 .................................... ..... 20 3.6.2 控制組之牙本質試片處理 ............................... 20 3.6.3 實驗組之牙本質試片處理 ............................... 20 3.6.4 臨界點乾燥 (Critical Point Drying) ................... 21 3.6.5 掃描式電子顯微鏡觀察 ................................ . 22 3.6.6 能量散射光譜分析儀觀察 ............................... 22 3.7 生物相容性測試 ................................ ............. 23 3.7.1 3T3 細胞株培養 ................................... ....... 23 3.7.2 材料萃取液之製備 ................................ ..... 23 3.7.3 WST-1細胞活性測試 ................................ ... 23 3.7.4 LDH 細胞毒性測試 ................................ ..... 24 第四章 結果與討論 ................................ .................. 25 4.1 BCP-玻璃粉末的性質分析 ................................ ..... 25 4.1.1 X 光繞射圖譜 ................................... ......... 25 4.1.2 穿透式電子顯微鏡觀察 ................................ . 26 4.1.3 能量散射光譜 分析儀觀察 ............................... 26 4.2 BCP-玻璃 /明膠製劑 ................................ .......... 29 4.2.1 BCP-玻璃 /明膠製劑之結果 .............................. 29 4.2.2 酸鹼值變化觀察 .................................... ....... 31 4.3 使用人類牙本質模型 對於牙本質小管的封閉深度之評估 .......... 33 4.3.1 掃描式電子顯微鏡的觀察 ............................... 33 4.3.2 能量分散光譜析 ................................ ..... 39 4.4 生物相容性測試 ................................ ............. 44 4.4.1 WST -1細胞活性測試 ................................ ... 44 4.4.2 LDH 細胞毒性測試 .................................. ..... 45 第五章 結論 .................................. ........................ 46 第六章 未來展望 ................................ .................... 47 參考文獻 ................................ ........................... 48 | |
| dc.language.iso | zh-TW | |
| dc.subject | 牙本質小管 | zh_TW |
| dc.subject | 生醫玻璃 | zh_TW |
| dc.subject | 微生物感染 | zh_TW |
| dc.subject | 根管治療 | zh_TW |
| dc.subject | 生醫玻璃 | zh_TW |
| dc.subject | 微生物感染 | zh_TW |
| dc.subject | 根管治療 | zh_TW |
| dc.subject | 牙本質小管 | zh_TW |
| dc.subject | bioactive glass | en |
| dc.subject | bioactive glass | en |
| dc.subject | dentin tubules | en |
| dc.subject | bacterial infection | en |
| dc.subject | root canal treatment | en |
| dc.subject | root canal treatment | en |
| dc.subject | bacterial infection | en |
| dc.subject | dentin tubules | en |
| dc.title | 鈣磷硼系玻璃於根管治療的應用 | zh_TW |
| dc.title | Application of CaO-P2O5-B2O3 Glass System on the Root Canal Treatment | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林俊彬(Chun-Pin Lin),楊禎明(Jen-Ming Yang),張國基(Kuo-Chi Chang) | |
| dc.subject.keyword | 根管治療,微生物感染,牙本質小管,生醫玻璃, | zh_TW |
| dc.subject.keyword | root canal treatment,bacterial infection,dentin tubules,bioactive glass, | en |
| dc.relation.page | 51 | |
| dc.identifier.doi | 10.6342/NTU201601081 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-07-21 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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