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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊鏡堂(Jing-Tang Yang) | |
dc.contributor.author | Cheng-Hau Yang | en |
dc.contributor.author | 楊程皓 | zh_TW |
dc.date.accessioned | 2021-06-15T12:41:10Z | - |
dc.date.available | 2016-08-24 | |
dc.date.copyright | 2016-08-24 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-07-27 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50448 | - |
dc.description.abstract | 本研究以micro-PIV分析液珠於流道中的流場行為,並探討液珠於十字交疊流道中的切割及融合行為,進行高通量液珠濃度調配。本研究之成果將可高通量產生多種濃度之液珠,並應用於細胞對藥物反應之檢測。
本研究調整連續相與分散相之黏性比比流量比,探討不同操作條件對液珠內部流場以及傳輸行為的影響,以做為液珠濃度調控設計之參考。在固定分散相及連續相黏性比時,降低分散相及連續相流量比將使液珠內部速度場增強;固定分散相及連續相流量比時,減少分散相及連續相黏性比將使得液珠內部渦度及環流量增大。在調配濃度方面,本研究所設計之流道可對液珠進行三階段的操控:首先利用T型流道生成液珠,並經過分岔流道加速;接著在兩流道的十字交疊流處,因流速差而改變流場分布,進而使液珠轉向,分割出不同大小的液珠;最後利用漸擴/漸縮流道將各自經過分割的液珠進行融合。此設計可使不同大小的藥物液珠與緩衝液液珠融合,達到含有目標藥物濃度之液珠。本研究最終可調控出原始濃度的28%~72%範圍內之液珠。 本研究以micro-PIV得到液珠內部流場資訊,並將其應用於液珠濃度之調控,未來可更深入探討十字交疊流道之流場變化機制,並將此研究成果應用於生醫與製藥等領域,將有助於減少浪費昂貴的試劑,以及高通量快速生成所需的濃度比例,若有多種濃度配比需求,則依需求調整流率以快速改變產生液珠之濃度。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-15T12:41:10Z (GMT). No. of bitstreams: 1 ntu-105-R03522309-1.pdf: 7704316 bytes, checksum: e3b8e3a0a0e568cb9bde9783d46208ba (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 摘要 iii 目錄 iv 圖表目錄 vi 符號說明 xii 第一章 前言 1 第二章 文獻回顧 3 2-1 微流體系統 (Microfludics System) 3 2-2 液珠式微流體 (Droplet-based Microfluidics) 5 2-2.1 液珠生成 5 2-2.2 被動式液珠生成 7 2-3 液珠操控 13 2-3.1 液珠分割 13 2-3.2 液珠融合 15 2-5 十字交疊流道 19 2-5.1 十字交疊流道中的流向 19 2-5.2 十字交疊流道中液珠分割 21 2-6 濃度調配晶片 22 2-7 液珠式微流體的的內流場 28 2-7.1 micro-PIV量測技術 28 2-7.2 micro-PIV應用 30 2-7.3 micro-PIV流場量測-z 軸方向速度場 34 2-7.4 micro-PIV流場量測-液珠融合流場 37 2-7.5 渦漩結構 39 2-8 文獻回顧分析 41 第三章 研究方法 42 3-1 研究架構 42 3-2 因次分析 43 3-3 微流體晶片 45 3-3.1 液珠式濃度調配晶片 45 3-3.2 流場探討晶片設計理念及流道繪製 48 3-3.2 母模製作-黃光微影製程 (photolithography) 51 3-3.3 晶片成形-PDMS元件翻製 58 3-4 流場觀測之實驗配置及分析方法 63 3-4.1 微粒子影像速度場量測技術 (micro-PIV) 介紹 63 3-4.2 實驗流程及儀器配置 66 3-5 實驗配置 71 3-5.1 觀測平台 71 3-5.2 實驗流程 72 第四章 結果與討論 74 4-1 液珠傳輸過程流場 74 4-1.1 拍攝流場各樣參數測試 74 4-1.2 液珠流場 77 4-1.3 流場觀測-固定黏性比,改變流量比 80 4-1.4 流場觀測-固定流量比,改變黏性比 82 4-2 十字交疊流道濃度調配 85 4-2.1 檢量線 85 4-2.2 實際濃度區間 89 第五章 結論與未來展望 94 5-1 結論 94 5-2 未來工作與展望 95 5-3 甘梯圖 96 第六章 參考文獻 97 | |
dc.language.iso | zh-TW | |
dc.title | 應用微液珠於十字交疊流道內流場行為進行濃度調配 | zh_TW |
dc.title | Concentration Proportioning and Flow Behavior of Droplets by Fission and Fusion in the Overlapped Microchannel | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 趙怡欽(Yei-Chin Chao),楊瑞珍(Ruey-Jen Yang),盧彥文(Yen-Wen Lu),廖英志(Ying-Chih Liao),周涵怡(Han-Yi Chou) | |
dc.subject.keyword | 液珠式微流體,晶片,微粒子影像測速,濃度調配, | zh_TW |
dc.subject.keyword | droplet-based microfluidic device,micro-PIV,Concentration Proportion, | en |
dc.relation.page | 100 | |
dc.identifier.doi | 10.6342/NTU201601346 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-07-28 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 機械工程學研究所 | zh_TW |
顯示於系所單位: | 機械工程學系 |
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