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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林俊彬 | |
dc.contributor.author | Yen-Yi Chen | en |
dc.contributor.author | 陳嬿伊 | zh_TW |
dc.date.accessioned | 2021-06-15T12:36:06Z | - |
dc.date.available | 2016-08-26 | |
dc.date.copyright | 2016-08-26 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-07-31 | |
dc.identifier.citation | Abe T, Taguchi, A., & Iwamoto, M. (1995). Non-silica-based mesostructured materials. 1. Synthesis of vanadium oxide-based materials. Chemistry of materials 7(8):1429-1430.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50316 | - |
dc.description.abstract | 本團隊之前的研究顯示以明膠為模板的含碳酸鈣中孔洞二氧化矽複合材料 (簡稱中孔洞鈣矽材料,GCMS) 與 30%磷酸調拌後,置於活體有牙髓腔靜水壓的牙本質上,可於牙本質小管內產生超過90μm的緻密結晶。於牙本質小管的成功經驗,讓本研究團隊開始思索這種中孔洞鈣矽材料是否能夠更進一步於根管系統內,尤其是側根管和根尖分岔,產生相同結果。另外因為中孔洞鈣矽材料含有矽,如果置於根管系統內不會因為再結晶而消失,若有其他不含矽的生醫活性材料能達到類似的牙本質小管再結晶效果亦是一大研究重點。經過文獻回顧發現一水磷酸二氫鈣(MCPM)與三鈣磷酸鹽(β-TCP)混合製劑可產生鈣磷結晶且用於骨頭修復已有多年。
因此本團隊進行以下的實驗: 首先觀察一水磷酸二氫鈣(MCPM)與三鈣磷酸鹽(β-TCP)混合製劑之不同酸鹼值於牙本質小管內再結晶,並且觀察MCPM/β-TCP混合製劑之時間長短對牙本質小管封閉效果,同時想藉由改變牙本質小管內層的醣聚醣胺結構對於生醫材料於牙本質小管封閉效果是否有所影響。進行離體人類牙齒根管系統實驗,評估生醫材料於牙齒根管系統的結晶狀態。最後進行動物實驗,以評估生醫材料在活體根管系統中的效用性。 第一部分以不同酸鹼值之MCPM/β-TCP混合製劑於牙本質試片上塗抹,再以電子顯微鏡觀察再結晶之情況,藉以篩選出適合後續實驗之組別。 第二部分將MCPM/β-TCP混合製劑塗抹放置於牙本質試片上之不同時間,比較時間因素對於再結晶之關係。 第三部分利用透明質酸酶與次氯酸鈉改變牙本質小管內層的醣聚醣胺結構,再分別塗抹上GCMS與MCPM/β-TCP混合製劑進行觀察。 第四部分將GCMS/磷酸製劑、MCPM/β-TCP製劑與生醫玻璃材料等三種生醫材料置於備製後離體人類牙齒之根管系統內,再以電子顯微鏡觀察側根管與根尖分岔再結晶之情況。 第五部分則進行動物實驗以評估GCMS/磷酸製劑、MCPM/β-TCP製劑與生醫玻璃材料等三種生醫材料在活體的效用性。 結論: 1. MCPM/β-TCP混合製劑於牙本質試片能形成再結晶,可有效封閉牙本質小管。其中莫耳比1:1,酸鹼值在3.5~4之混合製劑可在不明顯破壞牙本質結構下於牙本質小管內達到深度70μm的再結晶。材料放置時間與再結晶長度呈現正相關。 2. 透明質酸酶與次氯酸鈉可改變牙本質小管內層的醣聚醣胺結構,增進GCMS與MCPM/β-TCP混合製劑於牙本質小管內再結晶的深度。 3. GCMS、MCPM/β-TCP以及生醫玻璃材料在離體人類牙齒根管系統內可於側根管與根尖三分之一處牙本質小管內產生再結晶。 4. 於活體根管系統內MCPM/β-TCP混合製劑可於根尖三分之一處牙本質小管內產生再結晶。 | zh_TW |
dc.description.abstract | Our previous study showed that gelatin-templated calcium mesoporous silicate (GCMS) mixed with 30% H3PO4 could efficiently occlude dentinal tubules under pulpal pressure in beagle in vivo by precipitation of 90 μm calcium phosphate crystal. Whether there is same effect on root canal system, especially on lateral canal and apical ramifications, remains unknown. Besides, the silicate content may become concerned in root canal. Development of new bioactive material to precipitate in dentinal tubules is therefore another research goal. After review some literature, we assumed that monocalcium phosphate monohydrate (MCPM) and β-tricalcium phosphate (β-TCP) have potentials as GCMS.
Therefore, research goals of the present study is: First to observe different pH value in precipitation of MCPM/β-TCP mixture, and evaluation time factor in precipitation of MCPM/β-TCP mixture on dentinal tubules. At the same time, we modified inner surface of dentinal tubules and evaluation bioactive material on occlusion. Then evaluation GCMS, MCPM/β-TCP and DP-Bioglass in root canal system of human tooth in vitro. The last part was evaluated the efficacy in vivo by animal study This research comprised of five parts. First, we use different pH value of MCPM/β-TCP mixture place on dentin disc to observe precipitation by SEM and choice the most proper ratio for following study. The second part, we compare time factor and relation with precipitation in dentinal tubules. The third one is to modified inner surface of glycosaminoglycan in dentinal tubules by hyaluronidase and sodium hypochlorite and we evaluate the effect. Fourth, apply bioactive material including GCMS, MCPM/β-TCP and DP-Bioglass in root canal system of human tooth in vitro and observe by SEM. The final part we used animal study to evaluate the efficacy of GCMS, MCPM/β-TCP and DP-Bioglass in vivo. In conclusion: 1. MCPM/β-TCP can precipitate in dentinal tubule. Using the mole ratio of 1:1 and pH value 3.5 - 4, the precipitation reaches 70μm. The longer placed time is related to the better occlusion density and length. 2. Hyaluronidase and sodium hypochlorite can modify dentinal tubule and improve the depth of bioactive material precipitation in dentinal tubules. 3. GCMS, MCPM/β-TCP and DP-Bioglass can precipitate in the apical third of dentinal tubule and lateral canal in human tooth in vitro. 4. MCPM/β-TCP can also precipitation in apical third of dentinal tubule in vivo. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T12:36:06Z (GMT). No. of bitstreams: 1 ntu-105-R02422003-1.pdf: 6484366 bytes, checksum: 9b0dee7bbebc6c0ffd4ba59f2a656cf2 (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 目錄 8
第一章前言 第二章文獻回顧 2.1 根管系統與治療 16 2.2 次氯酸鈉之特性 17 2.3現行根管充填材料與缺點 17 2.4 牙本質結構 18 2.5 透明質酸(HYALURONIC ACID) 19 2.6 牙本質滲透性 (DENTIN PERMEABILITY) 19 2.7 生醫玻璃的應用 21 2.8 孔洞材料原理 22 2.8.1 中孔洞分子篩 22 2.8.2 中孔洞分子篩的性質與應用 24 2.9 奈米孔洞材料 25 2.9.1 中孔洞二氧化矽模板 25 2.9.2 結合金屬氧化物與中孔洞二氧化矽 26 2.10牙本質小管之離子通透性及選擇性 27 2.10.1 擴散傳送 (Diffusion transport) 27 2.10.2 對流傳送 (Convection transport) 28 2.10.3 牙齒硬組織的離子通透性 ( Ion permeability of dental hard 29 tissues) 29 2.11 牙本質小管帶電情形 29 2.12 一水磷酸二氫鈣與Β-三鈣磷酸鹽 30 2.12.1 結晶原理 30 2.12.2 磷酸氫鈣二水合物 31 2.13動物實驗 31 第三章 動機與目的 第四章 材料與方法 4.1 製備鈣磷生醫材料 33 4.1.1 以明膠為模版之中孔洞鈣矽材料製作 33 4.1.2 製備MCPM/β-TCP材料 34 4.1.3 製備實驗所需各種基本製劑 34 4.2 觀察及比較放置MCPM對於牙本質小管封閉效果 35 4.2.1牙本質試片之製備 35 4.2.2 MCPM不同時間對牙本質小管的封閉效果 35 4.2.3牙本質試片以掃描式電子顯微鏡(SEM) 觀察 36 4.3 觀察及比較放置MCPM/Β-TCP/磷酸根緩衝液之不同酸鹼值與比例對牙本質小管封閉效果 36 4.3.1 牙本質試片之製備 36 4.3.2評估放置MCPM/β-TCP/磷酸根緩衝液製劑之不同酸鹼值對牙本質小管的封閉效果 37 4.3.3評估放置MCPM/β-TCP/磷酸根緩衝液製劑之不同比例對牙本質小管的封閉效果 37 4.3.4牙本質試片以掃描式電子顯微鏡(SEM) 觀察 38 4.4 觀察及比較放置MCPM/Β-TCP/磷酸根緩衝液之不同時間對牙本質小管封閉效果 38 4.4.1 牙本質試片之製備 38 4.4.2 評估放置MCPM/β-TCP/磷酸根緩衝液製劑之時間對牙本質試片的影響 39 4.4.3 掃描式電子顯微鏡 (SEM) 進行樣本觀察 39 4.4.4 能量散射光譜分析儀 (EDS) 觀察 40 4.5移除牙本質小管內醣胺聚醣之觀察 41 4.5.1 透明質酸酶之作用 41 4.5.2 次氯酸鈉之作用 41 4.6 鈣磷材料放置於離體人類牙齒根管模型之觀察 41 4.6.1 微米級電腦斷層(Micro Computed Tomography )分析 4.6.2 離體人類牙齒根管模型之備製 42 4.6.3掃描式電子顯微鏡 (SEM) 進行樣本觀察 42 4.7 動物實驗 43 4.7.1 實驗動物之選擇與照顧 43 4.7.2 實驗動物之麻醉 43 4.7.3 實驗步驟 43 第五章實驗結果 5.1 MCPM材料之不同時間對牙本質小管封閉觀察 46 5.2 MCPM/Β-TCP/磷酸根緩衝液之不同酸鹼值與比例對牙本質小管封閉觀察 46 5.2.1 MCPM/β-TCP不同酸鹼值以掃描式電子顯微鏡 (SEM)樣本觀察 46 5.2.2 MCPM/β-TCP不同比例以掃描式電子顯微鏡 (SEM)樣本觀察 47 5.3 MCPM/Β-TCP/磷酸根緩衝液之時間長短對牙本質小管封閉效果觀察 48 5.3.1 MCPM/β-TCP以掃描式電子顯微鏡 (SEM)樣本觀察 48 5.3.2 MCPM/β-TCP以能量散射光譜分析儀 (EDS) 觀察 48 5.4 移除牙本質小管內醣胺聚醣之觀察 49 5.4.1 透明質酸酶之作用 49 5.4.2 次氯酸鈉之作用 50 5.5鈣磷材料放置於離體人類牙齒根管模型之觀察 51 5.5.1微米級電腦斷層觀察 51 5.5.2掃描式電子顯微鏡觀察 51 5.5.3能量散射光譜分析儀 (EDS)觀察 51 5.6動物實驗: 活體有效性測試 52 5.6.1微米級電腦斷層觀察側根管封閉效果 52 5.6.2掃描式電子顯微鏡觀察評估製劑對側根封閉效果 52 第六章討論 6.1 MCPM/Β-TCP混合製劑之作用 53 6.1.1 MCPM之作用 53 6.1.2 MCPM/β-TCP之酸鹼值 53 6.1.3 MCPM/β-TCP之比例 53 6.1.4 緩衝液之功能 54 6.1.5 材料塗抹於牙本質試片的結晶沉澱機制 54 6.2 移除牙本質小管內醣胺聚醣之觀察 54 6.2.1透明質酸酶之作用 54 6.2.2 次氯酸鈉之作用 55 6.3鈣磷材料放置於離體人類牙齒根管模型評估 55 6.3.1 中孔洞鈣矽材料對於側根管與分支的封閉效果 55 6.3.2 MCPM/β-TCP材料對於側根管與分支的封閉效果 55 6.3.3 生醫玻璃材料對於側根管與分支的封閉效果 56 6.3.4能量散射光譜分析儀 (EDS)觀察 56 6.4 動物實驗活體效用性 56 6.4.1中孔洞鈣矽材料對於側根管與分支的封閉效果 56 6.4.2 MCPM/β-TCP材料對於側根管與分支的封閉效果 56 6.4.3生醫玻璃材料對於側根管與分支的封閉效果 57 第七章 結論 58 第八章未來研究方向 59 第九章 參考文獻 60 | |
dc.language.iso | zh-TW | |
dc.title | 研發根管治療用生醫活性材料:結晶機制與封閉側根管及分支 | zh_TW |
dc.title | Development of bioactive materials for root canal therapy: crystallization mechanisms and occlusion of lateral canals and ramifications | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林弘萍,章浩宏 | |
dc.subject.keyword | 鈣磷生醫材料,MCPM/β-TCP,側根管與根尖分岔,醣聚醣胺,動物實驗, | zh_TW |
dc.subject.keyword | calcium phosphate biomaterial,MCPM/β-TCP,lateral canals and ramifications,Glycosaminoglycan,animal study, | en |
dc.relation.page | 96 | |
dc.identifier.doi | 10.6342/NTU201601399 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-08-01 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
顯示於系所單位: | 臨床牙醫學研究所 |
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