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完整後設資料紀錄
DC 欄位值語言
dc.contributor.advisor徐濟泰
dc.contributor.authorKai-Wei Huangen
dc.contributor.author黃凱瑋zh_TW
dc.date.accessioned2021-06-15T12:35:53Z-
dc.date.available2019-08-03
dc.date.copyright2016-08-03
dc.date.issued2016
dc.date.submitted2016-07-30
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dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50310-
dc.description.abstract本研究旨在探討不同黴菌毒素吸附劑吸附黃麴毒素B1(aflatoxin B1, AFB1)和嘔吐毒素(deoxynivalenol, DON)的能力。試驗使用不同劑量的無機吸附劑包含水合矽酸鋁鈣鈉(hydrated sodium calcium aluminosilicate, HSCAS)、沸石粉、竹炭粉和有機吸附劑包含瘤胃原蟲凍乾粉末、酵母粉分別給予0.1、0.2和0.5%之劑量,以及菌數為1010 CFU/mL的Lactobacillus rhamnosus strain GG(LGG)凍乾粉末進行個別1 ppm AFB1和2 ppm DON吸附測試,分別於磷酸鹽緩衝液(phosphate buffered saline, PBS)、模擬胃液(simulated gastric juice, SG)和模擬胃腸消化液(simulated gastrointestinal digestion, SGI)中比較吸附率。研究結果顯示AFB1吸附測試中,不管處於PBS、SG和SGI的狀態下,無機吸附劑吸附AFB1能力皆顯著大於有機吸附劑(P<0.05),而在有機吸附劑中,以LGG效果最好(P<0.05)。DON的吸附測試中,於PBS環境中,竹炭粉擁有最好的吸附能力(P<0.05),但當處於SGI中,以有機吸附劑和沸石粉有最好的吸附能力(P<0.05),但所有吸附劑在SG和SGI中對於DON的吸附能力均不達30%。綜述論之,於AFB1吸附測試中,無機吸附劑吸附能力大於有機吸附劑,但DON的吸附測試中,所有吸附劑對於DON的吸附能力皆不彰,因此有必要往其他吸附劑或是其他去毒方式研究。zh_TW
dc.description.abstractThe objective of this study was to test on the selected common mycotoxin adsorbents to adsorb aflatoxin B1 (AFB1) and deoxynivalenol (DON). Inorganic adsorbents (HSCAS, zeolite and bamboo charcoal) and organic adsorbents (rumen protozoa and yeast powder) were given 0.1, 0.2 and 0.5% in test. Lactobacillus rhamnosus strain GG(LGG)were given 1010 CFU/mL. All groups were tested for their ability to adsorb 1 ppm AFB1 and 2 ppm DON in PBS, simulated gastric juice (SG) and simulated gastrointestinal digestion (SGI), respectively. Results indicated that inorganic adsorbents had better ability to adsorb AFB1 than organic adsorbents (P<0.05) in PBS, SG and SGI. In organic adsorbents, LGG had the best ability to adsorb AFB1. In the test of DON adsorption, bamboo charcoal had the best ability to adsorb DON in PBS. But, zeolite and organic adsorbents had the best ability to adsorb DON in SGI. However, all adsorbents had lower than 30% binding levels of DON in SG and SGI. In conclusion, inorganic adsorbents had better ability to adsorb AFB1 than organic adsorbents. All adsorbents had inferior binding capability of DON. It is necessary to find other adsorbent or detoxification method to remove DON from feeds.en
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dc.description.tableofcontents謝誌 I
摘要 II
ABSTRACT III
目錄 IV
圖次 VII
表次 VIII
緒言 1
壹、文獻探討 2
一、黴菌毒素 2
(一)黃麴毒素 3
(二)黃麴毒素B1之毒性 8
(三)嘔吐毒素 13
(四)嘔吐毒素之毒性 17
二、各國規範黃麴毒素以及嘔吐毒之限量標準 21
三、黴菌毒素去毒方法 25
(一)物理法 25
(二)化學法 26
(三)生物法 27
四、吸附劑 28
(一)無機吸附劑 29
(二)有機吸附劑 31
五、反芻動物對於黴菌毒素的抵抗力 33
六、吸附劑吸附毒素的體外實驗和體內實驗的差異 33
七、黃麴毒素與嘔吐毒素在臺灣畜牧產業上的重要性 34
貳、材料與方法 35
一、黴菌毒素吸附劑 35
(一)瘤胃原蟲之製備 35
(二)乳酸菌之製備 35
(三)其餘吸附劑 35
二、試驗方法 36
(一)不同吸附劑於磷酸鹽緩衝液對黃麴毒素之吸附測試 36
(二)不同吸附劑於模擬胃液液對黃麴毒素之吸附測試 36
(三)不同吸附劑於模擬胃腸消化對黃麴毒素之吸附測試 37
(四)不同吸附劑於磷酸鹽緩衝液對嘔吐毒素之吸附測試 37
(五)不同吸附劑於模擬胃液液對嘔吐毒素之吸附測試 37
(六)不同吸附劑於模擬胃腸消化對嘔吐毒素之吸附測試 37
三、毒素分析方法 38
(一)黃麴毒素分析 38
(二)嘔吐毒素分析 39
四、統計分析 39
参、結果 40
肆、討論 52
伍、結論 59
參考文獻 60
小傳 75
dc.language.isozh-TW
dc.subject瘤胃原蟲zh_TW
dc.subject吸附劑zh_TW
dc.subject黴菌毒素zh_TW
dc.subject有機吸附劑zh_TW
dc.subject黃麴毒素zh_TW
dc.subject嘔吐毒素zh_TW
dc.subject黴菌毒素zh_TW
dc.subject吸附劑zh_TW
dc.subject瘤胃原蟲zh_TW
dc.subject有機吸附劑zh_TW
dc.subject黃麴毒素zh_TW
dc.subject嘔吐毒素zh_TW
dc.subjectadsorbenten
dc.subjectmycotoxinen
dc.subjectrumen protozoaen
dc.subjectorganic adsorbenten
dc.subjectaflatoxinen
dc.subjectdeoxynivalenolen
dc.subjectmycotoxinen
dc.subjectadsorbenten
dc.subjectrumen protozoaen
dc.subjectorganic adsorbenten
dc.subjectaflatoxinen
dc.subjectdeoxynivalenolen
dc.title黴菌毒素清除劑對於黃麴毒素B1與嘔吐毒素吸附能力之比較zh_TW
dc.titleComparison of Adsorption Capacity of Mycotoxin Detoxification Agents on Aflatoxin B1 and Deoxynivalenolen
dc.typeThesis
dc.date.schoolyear104-2
dc.description.degree碩士
dc.contributor.oralexamcommittee魏恒巍,林美峰,劉?睿,王翰聰
dc.subject.keyword黴菌毒素,吸附劑,瘤胃原蟲,有機吸附劑,黃麴毒素,嘔吐毒素,zh_TW
dc.subject.keywordmycotoxin,adsorbent,rumen protozoa,organic adsorbent,aflatoxin,deoxynivalenol,en
dc.relation.page75
dc.identifier.doi10.6342/NTU201601610
dc.rights.note有償授權
dc.date.accepted2016-08-01
dc.contributor.author-college生物資源暨農學院zh_TW
dc.contributor.author-dept動物科學技術學研究所zh_TW
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