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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 戴榮湘(Jung-Hsiang Tai) | |
| dc.contributor.author | Yen-Yu Yang | en |
| dc.contributor.author | 楊硯宇 | zh_TW |
| dc.date.accessioned | 2021-06-15T11:48:41Z | - |
| dc.date.available | 2021-08-26 | |
| dc.date.copyright | 2016-08-26 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-08-12 | |
| dc.identifier.citation | 參考資料
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49792 | - |
| dc.description.abstract | 鐵離子目前已知可活化陰道滴蟲蛋白激酶A相關訊息傳遞路徑，造成Myb3轉錄因子快速入核後遂行其功能，研究更發現此寄生蟲在鐵離子刺激後可誘發組蛋白脫乙醯酶TvHADC1的Ser391位點磷酸化。本實驗則利用蛋白激酶抑制劑發現鐵離子刺激透過PKC及MEK相關訊號傳遞路徑調控TvHDAC1入核，但H2O2刺激則非。TvHDAC1的過量表現亦造成組蛋白H3的Lys9以及H4的Lys5、Lys8、Lys16低乙醯化，然此現象不見於TvHDAC1的S391A點突變轉殖蟲株。藉由質譜分析細胞核蛋白，除發現鐵離子刺激快速改變組蛋白乙醯化與甲基化狀態，且變化趨勢異於H2O2刺激結果，鐵離子刺激亦同時活化組蛋白乙醯轉移酶TvHAT1與TvHAT2以及另外兩個組蛋白脫乙醯酶TvHDAC2與TvHDAC3磷酸化。免疫螢光染色分析驗證陰道滴蟲受鐵離子刺激後，可快速調控組蛋白特定位點乙醯化修飾。綜合以上數據顯示，陰道滴蟲受鐵刺激後，可能藉由訊號傳遞調控組蛋白乙醯轉移酶與脫乙醯酶，改變組蛋白密碼以控制下游基因活性，快速調節細胞生理因應環境中過量鐵離子。 | zh_TW |
| dc.description.abstract | Iron was shown to rapidly activate protein kinase A-mediated signaling to induce nuclear influx of a Myb3 transcription factor in the protozoan parasite, Trichomonas vaginalis. Site-specific phosphorylation of a histone deacetylase (HDAC), referred to as TvHDAC1, at Ser391 was previously found right after iron repletion. Here, the underlying mechanism of nuclear import of TvHDAC1 induced by iron but not H2O2 was investigated through inhibitor assay and the results indicate that PKC and MEK-related signaling pathways were activated to induce translocation of the enzyme from the endomembrane system toward the nucleus. Overexpression of TvHDAC1 resulted in hypoacetylation of histone H3 at Lys9 and histone H4 at Lys5, Lys8, Lys16, but this effect was aborted in the dominant negative mutant S391A. Exploring nuclei purification for LC-MS/MS based proteomics analysis, iron was found to induce rapid changes of acetylation and methylation on histones in a distinct way from H2O2 stimulation, and phosphorylation of two histone acetyltransferases (HAT), referred to as TvHAT1 and TvHAT2, and two additional HDACs, referred to as TvHDAC2 and TvHDAC3. The immunofluorescence assay was employed to confirm deacetylation of histones at specific sites upon iron repletion. These observations suggest that iron rapidly orchestrates multiple histone code writers through signal transduction to fine-tune gene expression in the parasite in response to iron overloading. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T11:48:41Z (GMT). No. of bitstreams: 1 ntu-105-R03445201-1.pdf: 4126169 bytes, checksum: 4fe4e4ac5bc4d9d9fb31a2887fac1a28 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 口試委員審議書 i
誌謝 ii 目錄 1 附圖目錄 3 附表目錄 4 縮寫表 5 英文摘要 7 中文摘要 8 第一章 前言 9 第一節 陰道滴蟲簡介 9 第二節 鐵離子 10 第三節 陰道滴蟲與鐵離子 12 第四節 組蛋白轉譯後修飾 12 第五節 研究目的 15 第二章 材料與方法 16 第一節 陰道滴蟲蟲株 16 第二節 免疫螢光染色 17 第三節 質譜分析樣本製備 18 第四節 蛋白質聚丙烯醯胺凝膠電泳 22 第五節 西方轉漬 22 第六節 抑制劑試驗 22 第三章 結果 23 第一節 鐵離子誘發陰道滴蟲HDAC與HAT之磷酸化 23 第二節 陰道滴蟲TvHDAC1與鐵離子關係之探討 24 第三節 鐵離子誘導組蛋白轉譯後修飾 26 第四節 TvHDAC1之功能分析 27 第四章 討論 28 第一節 鐵離子快速誘發陰道滴蟲細胞核生理調控 28 第二節 TvHDAC1細胞內流動之探討 29 第三節 TvHDACs與TvHATs磷酸化位點探討 31 第四節 陰道滴蟲受鐵或過氧化氫誘導之組蛋白轉譯後修飾 32 第五節 TvHDAC1功能性分析 34 第六節 總結 35 附圖 36 附表 61 參考資料 70 附錄 78 | |
| dc.language.iso | zh-TW | |
| dc.subject | 組蛋白脫乙醯? | zh_TW |
| dc.subject | 組蛋白 | zh_TW |
| dc.subject | 乙醯化 | zh_TW |
| dc.subject | 鐵 | zh_TW |
| dc.subject | 入核 | zh_TW |
| dc.subject | 陰道滴蟲 | zh_TW |
| dc.subject | iron | en |
| dc.subject | histone deacetylase | en |
| dc.subject | histone | en |
| dc.subject | Trichomonas vaginalis | en |
| dc.subject | acetylation | en |
| dc.title | 陰道滴蟲受鐵誘導之組蛋白轉譯後修飾 | zh_TW |
| dc.title | Iron-Inducible Histone Modifications in
Trichomonas vaginalis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 許弘明(Hong-Ming Hsu),許邦弘(Pang-Hung Hsu) | |
| dc.subject.keyword | 陰道滴蟲,組蛋白,組蛋白脫乙醯?,乙醯化,入核,鐵, | zh_TW |
| dc.subject.keyword | Trichomonas vaginalis,histone,histone deacetylase,acetylation,iron, | en |
| dc.relation.page | 106 | |
| dc.identifier.doi | 10.6342/NTU201602421 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-08-12 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-105-1.pdf 未授權公開取用 | 4.03 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。