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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 廖尉斯 | |
dc.contributor.author | Chia-Lin Chen | en |
dc.contributor.author | 陳家琳 | zh_TW |
dc.date.accessioned | 2021-06-15T11:48:03Z | - |
dc.date.available | 2019-10-14 | |
dc.date.copyright | 2016-10-14 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-08-12 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49782 | - |
dc.description.abstract | 紙質感測器由於低成本、易取得、高生物相容性以及環保等特色,在近年成為一熱門的檢測工具。然而,將螢光檢測技術應用於紙型平台經常會遭遇高背景值的問題,限制螢光在紙型感測器上的應用。因此我們結合紙質、具有大斯托克斯位移的銅奈米團簇以及熱塑膜封裝等設計來克服高背景值的問題,藉由半胱胺酸的偵測來展現此整合裝置的實際應用。當偵測樣品內存在半胱胺酸時會與銅奈米團簇反應產生螢光的變化,半胱胺酸的硫醇官能基會分解銅奈米團簇,因此從肉眼上觀察到螢光的消失。紙質感測器可利用數位相機作為偵測器量測螢光的變化,而拍攝的數位影像再利用軟體進行定量分析。紅綠藍顏色模型可有效地將銅奈米團簇的紅色螢光訊號從藍綠色背景值分離出來,不需使用任何光學濾光片,減少對精密光學配備的需求,並縮小背景值對偵測的干擾。此紙質平台與熱塑膜封裝以及抗氧化試片結合,可以有效地增長裝置的使用壽命至20天,並增強紙質的機械強度使其不易破碎。這一整合設計呈現低成本且不需任何光學濾光片的螢光紙質感測器,可藉由後續影像處理有效地降低在紙質平台的背景值並延長裝置壽命。 | zh_TW |
dc.description.abstract | Paper-based analytical device is a robust sensing platform due to their low cost, high availability, biocompatibility, and environmental friendlily properties. However, paper materials often exhibit a strong background signal under UV illumination, limiting their application with fluorescent detections. In this study, an approach combining paper material, copper nanocluster with a large Stokes shift, and thermoplastic lamination is utilized to resolve this problem without the requirement of expensive optical filter setups. The capability of this integrated device is established through cysteine detection by the use of synthesized copper nanoclusters. A digital camera is applied to capture fluorescence changes on the platform and a signal reduction via thiol group induced metal cluster etching is observed. Red-Green-Blue (RGB) color imaging analysis can effectively remove the fluorescence background in absence of optical filters, and the red emission signals from copper nanoclusters can be quantified. The incorporation of thermoplastic films and antioxidative sheets greatly improves device durability under ambient conditions and provides mechanical supports. This integrated design offers a durable, low-cost, optical filter-free, and highly-reduced background fluorescent sensing platform. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T11:48:03Z (GMT). No. of bitstreams: 1 ntu-105-R03223121-1.pdf: 2056781 bytes, checksum: 4379fb5aa34afc12e58976b932cc531a (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii Abstract iii List of Figures vi Chapter 1 Introduction 1 1.1 Paper-Fluidic Device 1 1.2 Fluorescent Paper-Based Sensing Platform 3 1.3 Metal Nanocluster 4 1.4 Copper Nanocluster 6 Chapter 2 Experimental Section 8 2.1 Materials 8 2.2 Synthesis of Red Fluorescence Emitting Copper Nanoclusters 8 2.3 Characterization of Copper Nanoclusters 9 2.4 Fabrication of Laminated Paper-Based Fluorescent Sensors 9 2.5 Analytical Assays with a Camera 11 Chapter 3 Results and Discussion 12 3.1 Characterization of BSA-Copper Nanoclusters 12 3.2 Cysteine Detection 18 3.3 Fluorescent Copper Nanocluster-Paper for Cysteine Detection 21 3.4 The Incorporation of Thermoplastic Films and Antioxidative Sheets 28 Chapter 4 Conclusions 30 References 31 | |
dc.language.iso | en | |
dc.title | 銅奈米團簇鑲嵌之紙質螢光感測平台 | zh_TW |
dc.title | Copper Nanocluster-Paper Laminated Filter-Free Fluorescent Sensing Platform | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 王宗興,黃志清,何美霖 | |
dc.subject.keyword | 紙型感測器,銅奈米團簇,螢光偵測,斯托克斯位移,紅綠藍顏色模型, | zh_TW |
dc.subject.keyword | paper-based analytical device,copper nanocluster,fluorescence detection,large Stokes shift,RGB color model, | en |
dc.relation.page | 40 | |
dc.identifier.doi | 10.6342/NTU201602197 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-08-12 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 化學研究所 | zh_TW |
顯示於系所單位: | 化學系 |
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