請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49329
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄭秋萍(Chiu-Ping Cheng) | |
dc.contributor.author | An-Chi Liu | en |
dc.contributor.author | 劉安琪 | zh_TW |
dc.date.accessioned | 2021-06-15T11:23:54Z | - |
dc.date.available | 2016-08-30 | |
dc.date.copyright | 2016-08-30 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-08-17 | |
dc.identifier.citation | Astua-Monge, G., Minsavage, G.V., Stall, R.E., Davis, M.J., Bonas, U., and Jones, J.B. (2000a). Resistance of tomato and pepper to T3 strains of Xanthomonas campestris pv. vesicatoria is specified by a plant-inducible avirulence gene. Mol. Plant-Microbe Interact. 13, 911-921.
Astua-Monge, G., Minsavage, G.V., Stall, R.E., Vellejos, C.E., Davis, M.J., and Jones, J.B. (2000b). Xv4-avrXv4a: a new gene-for-gene interaction identified between Xanthomonas campestris pv. vesicatoria race T3 and the wild tomato relative Lycopersicon pennellii. Mol. Plant-Microbe Interact. 13, 1346-1355. Baxter, A., Mittler, R. and Suzuki, N. (2014). ROS as key players in plant stress signalling. J. Exp. Bot. 65, 1229-1240. Bhattacharjee, S., Halane, M.K., Kim, S.H., and Gassmann, W. (2011). Pathogen effectors target Arabidopsis EDS1 and alter its interactions with immune regulators. Science 334, 1405-1408 Boatwright, J.L., and Pajerowska-Mukhtar, K. (2013). Salicylic acid: an old hormone up to new tricks. Mol. Plant Pathol. 14, 623-634. Boller, T., and Felix, G. (2009). A renaissance of elicitors: perception of receptors. Annu. Rev. Plant Biol. 60, 379-406. Bombarely, A., Rosli, H.G., Vrebalov, J., Moffett P., Mueller, L.A. and Martin, G.B. (2012). A draft genome sequence of Nicotiana benthamiana to enhance molecular plant-microbe biology research. Mol. Plant-Microbe Interact. 25, 1523- 1530. Böhm, H., Albert, I., Fan, L., Reinhard, A., and Nürnberger, T. (2014). Immune receptor complexes at the plant cell surface. Curr. Opin. Plant Biol. 20, 47-54. Brommonschenkel, S.H., Frary, A., Frary, A. and Tanksley, S.D. (2000). The broad- spectrum tospovirus resistance gene Sw-5 of tomato is a homolog of the root- knot nematode resistance gene Mi. Mol. Plant-Microbe Interact. 13, 1130-1138. Büttner, M. and Singh, K. B. (1997). Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA- binding protein interacts with an ocs element binding protein. Proc. Natl. Acad. Sci. USA 94, 5961-5966. Carmeille, A., Caranta, C., Dintinger, J., Prior, P., Luisetti, J., and Besse, P. (2006). Identification of QTLs for Ralstonia solanacearum race 3-phylotype II resistance in tomato. Theor. Appl. Genet. 113, 110-121. Chakravarthy, S., Tuori, R. P., D’Ascenzo, M. D., Fobert, P. R., Després, C., and Martin, G. B. (2003) The tomato transcription factor Pti4 regulates defense-related gene expression via GCC box and non-GCC box cis elements. Plant Cell 15, 3033-3050. Chang, C., Yu, D., Jiao, J., Jing, S., Schulze-Lefert, P., and Shen, Q.H. (2013). Barley MLA immune receptors directly interfere with antagonistically acting transcription factors to initiate disease resistance signaling. Plant Cell 25, 1158- 1173. Chen, Y.Y., Lin, Y.M., Chao, T.C., Wang, J.F., Liu, A.C., Ho, F.I., et al. (2009) Virus-induced gene silencing reveals the involvement of ethylene-, salicylic acid- and mitogen-activated protein kinase-related defense pathways in the resistance of tomato to bacterial wilt. Physiol. Plant. 136, 324-335. Cheng, M.C., Liao, P.M., Kuo, W.W. and Lin, T.P. (2013) The Arabidopsis ETHYLENE RESPONSE FACTOR1 regulates abiotic stress-responsive gene expression by binding to different cis-acting elements in response to different stress signals. Plant Physiol. 162, 1566-1582. Chung, E.H., El-Kasmi, F., He, Y., Loehr, A., and Dangl, J.L. (2014). A plant phosphoswitch platform repeatedly targeted by type III effector proteins regulates the output of both tiers of plant immune receptors. Cell Host Microbe 16, 484-494. Coemans, B., Takahashi, Y., Berberich, T., Ito, A., Kanzaki, H., Matsumura, H., et al. (2008). High-throughput in planta expression screening identifies an ADP- ribosylation factor (ARF1) involved in non-host resistance and R gene-mediated resistance. Mol. Plant Pathol. 9, 25-36. Coll, N.S., Epple, P., and Dangl, J.L. (2011). Programmed cell death in the plant immune system. Cell Death Differ. 18, 1247-1256. Cui, H., Tsuda, K. and Parker, J.E. (2015). Effector-triggered immunity: from pathogen perception to robust defense. Annu. Rev. Plant Biol. 66, 487-511. Curtis, M.K. and Grossniklaus, U. (2003). A gateway cloning vector set for high- throughput functional analysis of gene in planta. Plant Physiol. 133, 462-469. Dangl, J.L., Horvath, D.M. and Staskawicz, B.J. (2013). Pivoting the plant immune system from dissection to deployment. Science 341, 746-751. Deslandes, L., Olivier, J., Theulieres, F., Hirsch, J., Feng, D.X., Bittner-Eddy, P., Beynon, J. and Marco, Y. (2002). Resistance to Ralstonia solanacearum in Arabidopsis thaliana is conferred by the recessive RRS1-R gene, a member of a novel family of resistance genes. Proc. Natl. Acad. Sci. USA 99, 2404-2409. Deslandes, L., Olivier, J., Peeters, N., Feng, D.X., Khounlotham, M., Boucher, C., Somssich, I., Genin, S. and Marco, Y. (2003). Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus. Proc. Natl. Acad. Sci. USA 100, 8024-8029. Deslandes, L. and Rivas, S. (2012). Catch me if you can: bacterial effectors and plant targets. Curr. Opin. Plant Biol. 17, 644-655. Dickman, M.B. and Fluhr, R. (2013). Centrality of host cell death in plant-microbe interactions. Annu. Rev. Phytopathol. 51, 543-570. Ekengren, S.K., Liu, Y., Schiff, M., Dinesh-Kumar, S.P. and Martin, G.B. (2003). Two MAPK cascades, NPR1, and TGA transcription factors play a role in Pto- mediated disease resistance in tomato. Plant J. 36, 905-917. Fujimoto, S.Y., Ohta, M., Usui, A., Shinshi, H. and Ohme-Takagi, M. (2000). Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression. Plant Cell, 12, 393-404. Garcia A.V., Blanvillain-Baufume´, S., Huibers, R.P., Wiermer, M., Li, G., et al. (2010). Balanced nuclear and cytoplasmic activities of EDS1 are required for a complete plant innate immune response. PLOS Pathogen 6, e1000970. Gonzalez-Lamothe, R., Boyle, P., Dulude, A., Roy, V., Lezin-Doumbou, C., Kaur, G.S., et al. (2008). The transcriptional activator Pti4 is required for the recruitment of a repressosome nucleated by repressor SEBF at the potato PR-10a gene. Plant Cell 20, 3136-3147. Gu, Y. Q., Yang, C., Thara, V.K., Zhou, J. and Martin, G.B. (2000). Pti4 is induced by ethylene and salicylic acid, and its product is phosphorylated by the Pto kinase. Plant Cell 12, 771-785. Gu, Y. Q., Wildermuth, M. C., Chakravarthy, S., Loh, Y. T., Yang, C., He, X., et al. (2002). Tomato transcription factors Pti4, Pti5, and Pti6 activate defense responses when expressed in Arabidopsis. Plant Cell 14, 817-831. He, P., Warren, R. F., Zhao, T., Shan, L., Zhu, L., Tang, X., et al. (2001). Overexpression of Pti5 in tomato potentiates pathogen-induced defense gene expression and enhances disease resistance to Pseudomonas syringae pv. tomato. Mol. Plant-Microbe Interact. 14, 1453-1457. Hernández-Blanco, C., Feng, D.X., Hu, J., Sánchez-Vallet, A., Deslandes, L., Llorente, F., Berrocal-Lobo, M., Keller, H., Barlet, X., Sánchez-Rodríguez, C., Anderson, L.K., Somerville, S., Marco, Y. and Molina, A. (2007). Impairment of cellulose synthases required for Arabidopsis secondary cell wall formation enhances disease resistance. Plant Cell 19, 890-903. Hirsch, J., Deslandes, L., Feng, D.X., Balagué, C. and Marco, Y. (2002). Delayed symptom development in ein2-1, an Arabidopsis ethylene-insensitive mutant, in response to bacterial wilt caused by Ralstonia solanacearum. Phytopathol. 92, 1142-1148. Ishihama, N., Yamada, R., Yoshioka, M., Katou, S. and Yoshioka, H. (2011). Phosphorylation of the Nicotiana benthamiana WRKY8 transcription factor by MAPK functions in the defense response. Plant Cell 23, 1153-1170. Ishikawa, A., Tanaka, H., Nakai, M. and Asahi, T. (2003). Deletion of a chaperonin 60 beta gene leads to cell death in the Arabidopsis lesion initiation 1 mutant. Plant Cell Physiol. 44, 255-261. Jansson, S. (1994). The light-harvesting chlorophyll a/b-binding proteins. Biochim. Biophy. acta, 1184, 1-19. Jones J.B., Bouzar, H., Somodi, G.C., Stall, R.E., Pernezney, K., et al. (1988). Evidence for the preemptive nature of tomato race3 of Xanthomonas campestris pv. vesicatoria in Florida. Phytopathol. 88, 33-38 Jones, J.B., and Scott, J. W. (1986). Hypersensitive response in tomato to Xanthomonas campestris pv. vesicatoria. Plant Dis. 80, 337-339. Jones, J.B., and Stall, R.E. (1998). Diversity among xanthomonads pathogenic on pepper and tomato. Annu. Rev. Phytopathol. 36, 41-58. Kaneda, T., Taga, Y., Takai, R., Iwano, M., Matsui, H., et al. (2008). The transcription factor OsNAC4 is a key positive regulator of plant hypersensitive cell death. EMBO J. 28, 926-936. Kaufmann, K., Muiño, J.M. , Østerås, M. , Farinelli, L. , Krajewski, P. and Angenent, G.C. (2010). Chromatin immunoprecipitation (ChIP) of plant transcription factors followed by sequencing (ChIP-SEQ) or hybridization to whole genome arrays (ChIP-CHIP). Nat. Protoc. 5, 457-472. Kim M. G., da Cunha, L., McFall, A. J., Belkhadir, Y., DebRoy, S., et al. (2005). Two Pseudomonas syringae type III effectors inhibit RIN4-regulated basal defense in Arabidopsis. Cell 121, 749–759. Kim, S.H., Olson, T.N., Schaad, N.W., and Moorman, G.W. (2003). Ralstonia solanacearum race 3, biovar 2, the causal agent of brown rot of potato, identified in geraniums in Pennsylvania, Delaware, and Connecticut. Plant Dis. 87, 450. Lachaud, C., Prigent, E., Thuleau, P., Grat, S., Da Silva, D., Brière, C., Mazars, C. and Cotelle, V. (2013). 14-3-3-regulated Ca2+-dependent protein kinase CPK3 is required for sphingolipid-induced cell death in Arabidopsis. Cell Death Differ. 20, 209-217. Le Roux, C., Huet, G., Jauneau, A., Camborde, L., Tremousaygue, D., Kraut, A., Zhou, B., Levaillant, M., Adachi, H., Yoshioka, H., Raffaele, S., Berthomé, R., Couté, Y., Parker, J. E. and Deslandes, L. (2015). A receptor pair with an integrated decoy converts pathogen disabling of transcription factors to immunity. Cell 161, 1074-1088. Lee, L.Y., Wu, F.H., Hsu, C.T., Shen, S.C., Yeh, H.Y., Liao, D.C., Fang, M.J., Liu, N.T., Yen, Y.C., Dokládal, L., Sýkorová, E., Gelvin, S.B. and Lin, C.S. (2012). Screening a cDNA library for protein-protein interactions directly in Planta. Plant Cell 24, 1746-1759. Licausi, F., Ohme-Takagi, M. and Perata, P. (2013). APETALA2/Ethylene Responsive Factor (AP2/ERF) transcription factors: mediators of stress responses and developmental programs. New Phytol. 199, 639-649. Li, H., and Durbin, R. (2009). Fast and accurate short read alignment with Burrows-Wheeler Transform. Bioinformatics, 25, 1754-1760. Lin, Y.M., Shih, S.L., Lin, W. C., Wu, J.W., Chen, Y.T., Hsieh, C.Y., et al. (2014). Phytoalexin biosynthesis genes are regulated and involved in plant response to Ralstonia solanacearum infection. Plant Sci. 224, 86-94. Lorenzo, O., Piqueras, R., Sanchez-Serrano, J.J. and Solano, R. (2003). ETHYLENE RESPONSE FACTOR1 integrates signals from ethylene and jasmonate pathways in plant defense. Plant Cell 15, 165-178. Mackey, D., Belkhadir, Y., Alonso, J.M., Ecker, J.R., and Dangl, J.L. (2003). Arabidopsis RIN4 is a target of the type III virulence effector AvrRpt2 and modulates RPS2-mediated resistance. Cell 112, 379-789. Mase, K., Ishihama, N., Mori, H., Takahashi, H., Kaminaka, H., Kodama, M., et al. (2013). Ethylene-responsive AP2/ERF transcription factor MACD1 participates in phytotoxin-triggered programmed cell death. Mol. Plant- Microbe Interact. 26, 868-879. Matsumura, H., Reich, S., Ito, A., Saitoh, H., Kamoun, S., Winter, P., et al. (2003). Gene expression analysis of plant host-pathogen interactions by SuperSAGE. Proc. Natl. Acad. Sci. USA 100, 15718-15723. McHale, L., Tan, X., Koehl, P., and Michelmore, R.W. (2006). Pant NBS-LRR proteins: adaptable guards. Genome. Biol. 7, 212. McGrath, K.C., Dombrecht, B., Manners, J.M., Schenk, P.M., Edgar, C.I., Maclean, D.J., et al. (2005). Repressor- and activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of Arabidopsis transcription factor gene expression. Plant Physiol. 139, 949-959. Melech-Bonfil, S. and Sessa, G. (2010). Tomato MAPKKKε is a positive regulator of cell-death signaling networks associated with plant immunity. Plant J. 64, 379- 391. Meng, F. (2013). Ralstonia solanacearum species complex and bacterial wilt disease. J. Bacteriol. Parasitiol. 4, e119. Meng, X. and Zhang, S. (2013). MAPK cascades in plant disease resistance signaling. Annu. Rev. Phytopathol. 51, 245-266. Menke, F.L., Kang, H.G., Chen, Z., Park, J.M., Kumar, D., and Klessig, D.F. (2005). Tobacco transcription factor WRKY1 is phosphorylated by the MAP kinase SIPK and mediates HR-like cell death in tobacco. Mol. Plant-Microbe Interact. 18, 1027-1034. Mizoi, J., Shinozaki, K. and Yamaguchi-Shinozaki, K. (2012). AP2/ERF family transcription factors in plant abiotic stress responses. Biochim. Biophy. acta, 1819, 86-96. Moffat, C.S., Ingle, R.A., Wathugala, D.L., Saunders, N.J., Knight, H. and Knight, M.R. (2012). ERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis. PloS one, 7, e35995. Monaghan, J., and Zipfel, C. (2012). Plant pattern recognition receptor complexes at the plasma membrane. Curr. Opin. Plant Biol. 15, 349-357. Montillet, J.L., Chamnongpol, S., Rusterucci, C., Dat, J., van de Cotte, B., Agnel, J.P., et al. (2005). Fatty acid hydroperoxides and H2O2 in the execution of hypersensitive cell death in tobacco leaves. Plant Physiol. 138, 1516-1526. Muller, M. and Munne-Bosch, S. (2015). Ethylene Response Factors: A Key Regulatory Hub in Hormone and Stress Signaling. Plant Physiol. 169, 32-41. Nakano, T., Suzuki, K., Fujimura, T. and Shinshi, H. (2006). Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol. 140, 411-432. Naseem, M., Kaltdorf, M., and Dandekar, T. (2015). The nexus between growth and defence signalling: auxin and cytokinin modulate plant immune response pathways. J. Exp. Botany 16, 4885-4896. Nasir, K.H., Takahashi, Y., Ito, A., Saitoh, H., Matsumura, H., Kanzaki, H., et al. (2005). High-throughput in planta expression screening identifies a class II ethylene-responsive element binding factor-like protein that regulates plant cell death and non-host resistance. Plant J. 43, 491-505. Nguyen, H.P., Chakravarthy, S., Velasquez, A.C., McLane, H.L., Zeng, L., Nakayashiki, H., et al. (2010). Methods to study PAMP-triggered immunity using tomato and Nicotiana benthamiana. Mol. Plant-Microbe Interact. 23, 991-999. Ogata, T., Kida, Y., Tochigi, M., and Matsushita, Y. (2013). Analysis of the cell death-inducing ability of the ethylene response factors in group VIII of the AP2/ERF family. Plant Sci. 209, 12-23. Ogata, T., Okada, H., Kawaide, H., Takahashi, H., Seo, S., Mitsuhara, I., et al. (2015). Involvement of NtERF3 in the cell death signalling pathway mediated by SIPK/WIPK and WRKY1 in tobacco plants. Plant Biol. 17, 962-972. Oh, C.S., and Martin, G.B. (2011). Effector-triggered immunity mediated by Pto kinase. Trends Plant Sci. 16, 132-140. Ohme-Takagi, M., and Shinshi, H. (1995). Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. Plant Cell, 7, 173-182. Ohta, M., Matsui, K., Hiratsu, K., Shinshi, H., and Ohme-Takagi, M. (2001). Repression domains of class II ERF transcriptional repressors share an essential motif for active repression. Plant Cell, 13, 1959-1968. Onate-Sanchez, L., Anderson, J.P., Young, J. and Singh, K.B. (2007). AtERF14, a member of the ERF family of transcription factors, plays a nonredundant role in plant defense. Plant Physiol. 143, 400-409. Onate-Sanchez, L., and Singh, K.B. (2002). Identification of Arabidopsis ethylene- responsive element binding factors with distinct induction kinetics after pathogen infection. Plant Physiol. 128, 1313-1322. Pieterse, C.M., Van der Does, D., Zamioudis, C., Leon-Reyes, A., and Van Wees, S.C. (2012). Hormonal modulation of plant immunity. Annu. Rev. Cell Dev. Biol. 28, 489-521. Popoff, V., Adolf, F., Brugger, B., and Wieland, F. (2011). COPI budding within the Golgi stack. Cold Spring Harbor Persp. Biol. 3, a005231. Raffaele, S., Vailleau, F., Leger, A., Joubes, J., Miersch, O., Huard, C., et al. (2008). A MYB transcription factor regulates very-long-chain fatty acid biosynthesis for activation of the hypersensitive cell death response in Arabidopsis. Plant Cell, 20, 752-767. Roden, J., Eardley, L., Hotson, A., Cao, Y., and Mudgett, M.B. (2004). Characterization of the Xanthomonas AvrXv effector, a SUMO protease translocated into plant cells. Mol. Plant-Microbe Interact. 17, 633-643. Roux, F., Voisin, D., Badet, T., Balague, C., Barlet, X., Huard-Chauveau, C., et al. (2014). Resistance to phytopathogens e tutti quanti: placing plant quantitative disease resistance on the map. Mol. Plant Pathol. 15, 427-432. Sarris, P.F., Duxbury, Z., Huh, S.U., Ma, Y., Segonzac, C., Sklenar, J., Derbyshire, P., Cevik, V., Rallapalli, G., Saucet, S.B., Wirthmueller, L., Menke, F.L., Sohn KH3 and Jones, J.D. (2015). A Plant Immune Receptor Detects Pathogen Effectors that Target WRKY Transcription Factors. 161, 1089-1100. Sasaki, K., Mitsuhara, I., Seo, S., Ito, H., Matsui, H., and Ohashi, Y. (2007). Two novel AP2/ERF domain proteins interact with cis-element VWRE for wound- induced expression of the Tobacco tpoxN1 gene. Plant J. 50, 1079-1092. Scott J.W., Stall, R.E., Jones, J.B., and Somodi, G.C. (1996). A single gene controls the hypersensitive response of Hawaii 7981 to race 3 of the bacterial spot pathogen. Tomato Genetic Coop. 46, 16 Sharma, M.K., Kumar, R., Solanke, A.U., Sharma, R., Tyagi, A.K. and Sharma, A.K. (2010). Identification, phylogeny, and transcript profiling of ERF family genes during development and abiotic stress treatments in tomato. Mol. Genet. Genomics, 284, 455-475. Shen Q. H,, Saijo, Y., Mauch, S., Biskup, C., Bieri, S., et al. (2007). Nuclear activity of MLA immune receptors links isolate-specific and basal disease-resistance responses. Science 315, 1098-1103 Spassova, M.I., Prins, W., Folkertsma, R.T., Klein-Lankhorst, R.M., Hille, J., Goldbach, R.W., et al. (2001). The tomato gene Sw5 is a member of the coiled coil, nucleotide binding, leucine-rich repeat class of plant resistance genes and confers resistance to TSWV in tobacco. Mol. Breed 7, 151-161. Scott, J.W., and Jones, J.B. (1989). Inheritance of resistance to foliar bacterial spot of tomato incited by Xanthomonas campestris pv. vesicatoria. J. Am. Soc. Hort. Sci. 114, 111-114. Stall, R.E., Jones, J.B., and Minsavage, G.V. (2009). Durability of resistance in tomato and pepper to xanthomonads causing bacterial spot. Annu. Rev. Phytopathol. 47, 265-284. Tao, Y., Xie, Z., Chen, W., Glazebrook, J., Chang, H.S., Han, B., et al. (2003). Quantitative nature of Arabidopsis responses during compatible and incompatible interactions with the bacterial pathogen Pseudomonas syringae. Plant Cell, 15, 317-330. Teper, D., Salomon, D., Sunitha, S., Kim, J.G., Mudgett, M.B., and Sessa, G. (2014). Xanthomonas euvesicatoria type III effector XopQ interacts with tomato and pepper 14-3-3 isoforms to suppress effector-triggered immunity. Plnat J. 77, 297- 309. Thomas, H. (2013). Senescence, ageing and death of the whole plant. New Phytol. 197, 696-711. Tian, D., Wang, J., Zeng, X., Gu, K., Qiu, C., et al. (2014). The rice TAL effector- dependent resistance protein XA10 triggers cell death and calcium depletion in the endoplasmic reticulum. Plant Cell 26, 497-515. Wang. J.F., Ho, F.I., Truong, H.T.H., Huang, S.M., Balatero, C.H., Dittapongpitch, V., and Hidayati, N. (2013). Identification of major QTLs associated with stable resistance of tomato cultivar ‘Hawaii7996’ to Ralstonia solacearum. Euphytica 190, 241-252. Whalen, M.C., Wang, J.F., Carland, F.M., Heiskell, M., Dahlbeck, D., et al. (1993). Avirulence gene avrRxv from Xanthomonas campestris pv. vesicatoria specifies resistance on tomato line Hawaii 7998. Mol. Plant-Microbe Interact. 6, 616-627. Wrazaczek, M., Brosche, M., and Kangasjarvi, J. (2013). ROS signaling loops - production, perception, regulation. Curr. Opin. Plant Biol. 16, 575-582. Yu, Z.H., Wang, J.F., Stall, R.E., and Vallejos, C.E. (1995). Genomic localization of tomato genes that control a hypersensitive reaction to Xanthomonas campestris pv. vesicatoria (Doidge) Dye. Genetics 141, 675-682. Zhang, H., Huang, L., Dai, Y., Liu, S., Hong, Y., Tian, L., et al. (2015). Arabidopsis AtERF15 positively regulates immunity against Pseudomonas syringae pv. tomato DC3000 and Botrytis cinerea. Front. Plant Sci. 6, 686. Zhang, H., Li, W., Chen, J., Yang, Y., Zhang, Z., Zhang, H., et al. (2007). Transcriptional activator TSRF1 reversely regulates pathogen resistance and osmotic stress tolerance in tobacco. Plant Mol. Biol. 63, 63-71. Zhang, H., Zhang, D., Chen, J., Yang, Y., Huang, Z., Huang, D., et al. (2004). Tomato stress-responsive factor TSRF1 interacts with ethylene responsive element GCC box and regulates pathogen resistance to Ralstonia solanacearum. Plant Mol. Biol. 55, 825-834. Zhou, J., Tang, X., and Martin, G.B. (1997). The Pto kinase conferring resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis-related genes. EMBO J. 16, 3207-3218. Zhu, J.Y., Sun, Y., and Wang, Z.Y. (2012). Genome-wide identification of transcription factor-binding sites in plants using chromatin immunoprecipitation followed by microarray (ChIP-chip) or sequencing (ChIP-seq). Methods Mol. Biol. 876, 173-188. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49329 | - |
dc.description.abstract | Ethylene responsive factor (ERF)為一群植物特有之轉錄因子家族,於植物生長發育與逆境反應中,扮演相當重要之角色,但在番茄中仍有許多ERF之功能尚未明。於本研究中,我們針對一群屬於ERF-IX次族群但功能未知之ERF進行研究。其中,ERF68能被不同之細菌病原菌、防禦相關賀爾蒙乙烯及水楊酸大量誘導其基因表現;但是在第三型分泌系統缺失之突變株與非寄主性病原菌之處理下,誘導表現量卻大量下降,顯示病菌第三型效應蛋白可能為誘發ERF68大量表現之主要因子。利用螢光蛋白質融合定位法發現ERF68座落於細胞核內,同時利用Transactivation assay與electrophoretic mobility shift assays (EMSA)分析確認其具有轉錄因子之活性與功能。再者,利用病毒誘導基因過量表現也發現,大量表達ERF68基因可於番茄及菸草中誘發自發性細胞死亡並加強參與在乙烯、水楊酸、茉莉酸、抗病性過敏反應路徑之相關標誌基因表現。同時利用病毒誘發基因靜默法觀察ERF68靜默後之番茄對病原菌抗性反應,也顯示ERF68會降低對低致病力的茄科細菌性斑點病菌(bacterial spots)之抗性,但是對細菌性葉斑病菌(bacterial speck)或萎凋病(bacterial wilting)之抗性則無影響或影響輕微。此結果顯示ERF68應參與在番茄之effector-triggered immunity (ETI)抗病反應上。更進一步,為鑑定受ERF68所調控之下游標的基因,我們也利用染色質免疫沉澱法搭配高通量次世代定序法(ChIP-seq),解析可能之受調控標的基因。而此結果也顯示一些可能調控細胞死亡與植物防禦反應上的基因可直接受ERF68調控,進而參與在抗病反應中。總和上述結果,本研究證明番茄ERF68經由不同的反應路徑而正向調控過敏性細胞死亡與防禦反應,並提供ERF轉錄因子調控之訊息傳導網路研究一些新的資訊。 | zh_TW |
dc.description.abstract | Ethylene response factors (ERFs) are a large plant-specific transcription factor family and play diverse important roles in various plant functions. However, most tomato ERFs are not characterized. In this study, we showed that expression of an uncharacterized member of tomato ERF-IX subgroup, ERF68, was significantly induced by treatments of different bacterial pathogens, ethylene (ET) and salicylic acid (SA), but only slightly induced by bacterial mutants defective in the type III secretion system (T3SS) or non-host pathogens. ERF68-GFP fusion protein localized in the nucleus. Transactivation and electrophoretic mobility shift assays (EMSA) further showed that ERF68 was a functional transcriptional activator and bound to the GCC-box. Moreover, transient overexpression of ERF68 led to spontaneous lesions in tomato and tobacco leaves and enhanced expression of genes involved in ET, SA, jasmonic acid (JA) and hypersensitive response (HR) pathways, while silencing of ERF68 increased tomato susceptibility to two incompatible Xanthomonas spp. These results reveal the involvement of ERF68 in effector-triggered immunity (ETI) pathway. To identify ERF68 target genes, chromatin-immunopreciptation combined high-throughput sequencing (ChIP-seq) was performed. Among the confirmed target genes, a few genes involved in cell death or disease defense were differentially regulated by ERF68. Our study demonstrates function of ERF68 in positively regulating hypersensitive cell death and disease defense by modulating multiple signaling pathways, and provides important new information on the complex regulatory function of ERFs. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T11:23:54Z (GMT). No. of bitstreams: 1 ntu-105-D96b42009-1.pdf: 4891681 bytes, checksum: ae79c8d1b7944b557c28290552142cad (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 誌謝……………………………………………………………………………………i
中文摘要……………………………………………………………………………ii Abstract………………………………………………………………………………iv Abbreviations…………………………………………………………………………vi Contents………………………………………………………………………………ix Chapter 1 Introduction…………………………………………………………1 1.1 PAMP triggered immunity and effector trigger immunity……………….1 1.2 Bacterial wilting…………………………………………………………3 1.3 Bacterial spots……………………………………………………………5 1.4 Cell death…………………………………………………………………7 1.5 Ethylene responsive factor………………………………………………9 1.6 The aims of this study……………………………………………………12 Chapter 2 Materials and Methods……………………………………………14 2.1 Plant materials, bacterial strains and growth conditions…………………14 2.2 Cloning and construction of tomato ERF68……………………………15 2.3 Treatments and transcriptional analyses for tomato ERF68 expression….15 2.4 Protein localization and transactivation assay……………………………16 2.5 Recombinant protein construction and purification………………………17 2.6 Electrophoretic mobility shift assay (EMSA)…………………………….18 2.7 Virus-induced gene silencing (VIGS) and virus-mediated gene overexpression (VMGO)…………………………………………………19 2.8 Induced protein expression and analyses of cell conductivity and H2O2 accumulation……………………………………………………………...20 2.9 Assessment of plant disease responses…………………………………...21 2.9.1 Assessment of Pst infection………………………………………….21 2.9.2 Assessment of Xanthomonas spp…………………………………….22 2.9.3 Assessment of Rs …………………………………………………….23 2.9.4 Assessment of drought tolerance in tomato L390……………………24 2.10 Chromatin immunoprecipitation combined with high through-put sequence (ChIP-seq) and ChIP-qPCR…………………………………24 2.11 Bioinformatic analysis of ChIP-seq results……………………………26 Chapter 3 Results………………………………………………………………28 3.1 Expression of tomato ERF68 is induced by bacterial pathogens…………28 3.2 Tomato ERF68 is a transcriptional activator and binds to the GCC- box………………………………………………………………………29 3.3 Expression of tomato ERF68 causes spontaneous cell death…………30 3.4 Expression of tomato ERF68 enhances multiple defense signaling pathways………………………………………………………………31 3.5 Tomato ERF68 plays a role in defense responses involved in specific pathosystems……………………………………………………………32 3.6 Identification of ERF68 targets by ChIP-seq…………………………34 3.7 ERF68-dependent differentially expression of target genes……………36 Chapter 4 Discussion…………………………………………………………37 4.1 Tomato ERF68 is a pathogen-induced regulator controlling multiple defense signaling pathways and cell death……………………………37 4.2 ERF68 differentially regulates its target genes and may bind to various cis-elements……………………………………………………………39 4.3 ERF68 regulates genes involved in hypersensitive cell death and disease defense…………………………………………………………………41 4.4 Tomato ERF68 participates in ETI against incompatible Xanthomonas spp………………………………………………………………………43 4.5 Conclusion………………………………………………………………44 Chapter 5 Reference……………………………………………………………46 Tables………………………………………………………………………………63 Table 1. Summary of ChIP-seq assay in N. benthamiana leaves with induced transient expression of SlERF68-3×HA………………………………………63 Table 2. Summary of annotated scaffolds with putative SlERF68-binding region on promoters or transcriptional start sites……………………………………64 Figures………………………………………………………………………………69 Figure. 1 Protein alignment and phylogenetic analysis of selected members of ERF group IX…………………………………………………………………69 Figure. 2 Tomato ERF68 is induced by pathogens and hormones……………71 Fig. 3 The expression pattern of tomato ERF56, 58, 67 and 69 by pathogens treatment………………………………………………………………………73 Figure. 4 Tomato ERF68 is differentially expressed in different tissues………74 Figure. 5 ERF68 is localized in the nucleus and functions as a transcription factor…………………………………………………………………………75 Figure. 6 Overexpression of ERF68 causes spontaneous lesions and induces expression of defense-related genes in tomato H7996………………………77 Figure. 7 Overexpression of ERF68 causes spontaneous cell death in N. bemtahmiana and tobacco……………………………………………………79 Figure. 8 Induced ERF68 overexpression leads to cell death………………80 Figure. 9 Silencing of ERF68 does not affect tomato defense response to Pseudomonas syringae pv. tomato DC3000…………………………………82 Figure. 10 Silencing of ERF68 alters tomato defense responses to Xanthomonas spp……………………………………………………………………………84 Figure. 11 Silencing of ERF68 in susceptible tomato L390 leads to delayed bacterial wilt caused by Ralstonia solanacearum……………………………86 Figure. 12 Efficiency Optimization of cross-linking and DNA fragmentation in ChIP sample of N. bemthamiana leaves………………………………………87 Figure. 13 Validation of ERF68 binding to the promoters of the target genes by ChIP-qPCR analysis…………………………………………………………88 Figure. 14 ERF68 differentially regulates the target genes……………………89 Supplementary Tables…………………………………………………………91 Table S1. Differential reactions of races of xanthomonads on tomato resistance genes…………………………………………………………………………...91 Table S2. Primers and probes used in this study………………………………92 Table S3. Media and solutions used in this study……………………………96 Table S4. Assessment of Ralstonia solanacearum density in silenced tomato H7996 plants………………………………………………………………102 Supplementary figures………………………………………………………………103 Figure S1. ERF-IX is localized in the nucleus and functions as a transcription factor…………………………………………………………………………103 Figure S2. Validation of transient silencing and overexpression of tomato ERF68 in tomato H7996………………………………………………………………105 Figure S3. Drought response of tomato plants with silencing of selected ERF-VIII genes.…………………………………………………………………….107 Figure S4. A hypothesized model for ERF68’s involvement in incompatible tomato-Xanthomonas interactions……………………………………………109 Publications…………………………………………………………………………110 | |
dc.language.iso | en | |
dc.title | 病菌誘發之番茄轉錄因子ERF68調控過敏性細胞死亡及防禦反應 | zh_TW |
dc.title | Pathogen-induced ERF68 regulates hypersensitive cell death and defense response in tomato (Solanum lycopersicum L.) | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 鄭石通(Shih-Tong Jeng),吳克強(Ke-qiang Wu),陳昭瑩(Chao-Ying Chen),詹明才(Ming-Tsair Chan),葉信宏(Hsin-Hung Yeh) | |
dc.subject.keyword | 番茄,Ethylene responsive factor,細胞死亡,防禦反應,細菌性斑點病, | zh_TW |
dc.subject.keyword | Tomato,ERF,cell death,disease,defense, | en |
dc.relation.page | 115 | |
dc.identifier.doi | 10.6342/NTU201602713 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-08-18 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-105-1.pdf 目前未授權公開取用 | 4.78 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。