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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 郭典翰(Dian-Han Kuo) | |
dc.contributor.author | Yu-Hsiang Hsiao | en |
dc.contributor.author | 蕭宇翔 | zh_TW |
dc.date.accessioned | 2021-06-15T11:21:28Z | - |
dc.date.available | 2019-08-30 | |
dc.date.copyright | 2016-08-30 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-08-18 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49267 | - |
dc.description.abstract | 早期不同物種之間演化的研究,以物種的形態及生理特徵為依據,用此證明物種之間的親緣關係。兩側對稱動物的胚胎發育過程中,將腸道區域化細分成口部、前腸、中腸以及後腸區域。腸道的結構依照物種適應攝食機制的演化,而有所不同。隨著發育生物學的興起,藉由分子機制的研究發現,在後生動物胚胎endomesoderm中許多轉錄因子保守地參與調節,其中包含了與腸道發育相關的FoxA。因此,藉由同源基因所繪製的系統發生樹,以分子演化的方式預測物種之間的親緣關係,並藉由FoxA的研究探討對於物種腸道發育中的影響。
FoxA全名為Forkhead box A,屬於轉錄因子Fox gene家族,Fox genes早期文獻又稱fork head或HNF-3。在研究文獻指出,Fox家族調控著在胚胎發育過程中內胚層、肝臟、肺臟、以及腹側神經發育的形成。FoxA直向同源的基因在後口動物中,已被證實在腸道發育過程中扮演重要角色,並參與胚胎軸向結構的形成。在原口動物中,FoxA直向同源的基因對於前腸、中腸及後腸的正確發育具有影響力,且在胚胎發育成口部區域有FoxA基因的表現。 本研究以冠輪動物環節動物門的澤蛭Helobdella robusta為研究對象,搭配模式生物間系統發生樹的分析,利用原位雜合技術觀察轉錄因子FoxA在澤蛭胚胎發育前期、中期、晚期過程中,FoxA對於澤蛭胚胎發育過程中時間及空間上所扮演的角色。實驗結果發現,在澤蛭胚胎發育早期,Hro-FoxA3表現在endomesoderm當中,與多毛類在卵裂過程中植物極的表現一致。Hro-FoxA2表現在前腸與後腸區域為FoxA的保守功能。Hro-FoxA1表現於特定神經前趨細胞與在第九時期的Hro-FoxA3表現於腹側神經索處,意味著澤蛭的FoxA同源基因扮演著不同的功能。 | zh_TW |
dc.description.abstract | According to morphological and physiological characteristics of species to improve genetic relationship in early evolutionary study . Regionalization of gut into pharynx, foregut, midgut and hindgut in Bilateria embryonic development. In recent years, developmental biologists use genetic and molecular techniques to study the embryos development. Orthologs of regulatory genes that specify endomesoderm have different developmental roles in Metazoa. Polychaete annelids have conserved gene expression of transcription factors such as FoxA in endomesoderm. Therefore, study of molecular development can predict phylogenetic relationships and figure out gut formation in embryonic development.
Transcription factor Forkhead box A (FoxA) is a Fox gene family member. Fox family regulates embryonic development in endoderm, liver, lung, and the formation of the ventral neural development. In protostomes, FoxA orthologs gene has conserved expression in pharynx, forgut, midgut and hindgut region. FoxA orthologs gene not only plays an important role in gut development, but also involved ventral nerve cord formation in deuterostomes. This study use a in situ hybridization to observe expression patterns of FoxA genes during embryonic development of the leech Helobdella. The results show that Hro-FoxA3 express in endomesoderm during cleavage stage. Hro-FoxA2 conservatively express in foregut and hindgut region. Hro-FoxA1 express in specific neuronal precursor cells and Hro-FoxA3 express in ventral nerve cord during late stage. It is means FoxA orthologs play different role during leech embryonic development. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T11:21:28Z (GMT). No. of bitstreams: 1 ntu-105-R00b41024-1.pdf: 2254268 bytes, checksum: 8698a4680f423e94c92f66bff190f359 (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 誌謝 ii
中文摘要 iii 英文摘要 iv 第一章 序論 1 1.1 Forkhead box A 1 1.2 FoxA的功能 3 1.3 FoxA對於腸道發育形成的影響 4 1.4 澤蛭Helobdella的分類地位 6 1.5 澤蛭胚胎發育 7 1.5.1澤蛭胚胎發育早期 7 1.5.2澤蛭胚胎發育中期 8 1.5.3澤蛭胚胎發育後期 8 1.6 研究動機與目的 9 第二章 實驗材料與方法 11 2.1 澤蛭胚胎培養及選擇 11 2.2 系統發生樹(Phylogenetic tree) 11 2.3 原位雜合(In Situ Hybridization, ISH)技術 12 2.3.1 探針合成 12 2.3.2 胚胎固定及原位雜合前處理 13 2.3.3 雜合 13 2.3.4 探針移除 14 2.3.5 抗體黏合及抗體清洗 14 2.3.6 抗體呈色 14 2.3.6.1 BM Purple呈色法 14 2.3.6.2 NBT/BCIP呈色法 15 2.3.7 原位雜合結果觀察 15 第三章 實驗結果 16 3.1 模式物種間Fox基因家族親緣關係分析 16 3.2 FoxA在澤蛭胚胎發育過程中的時空變化 16 3.2.1 FoxA在澤蛭胚胎卵裂期的基因表現 17 3.2.2 FoxA在澤蛭原腸胚的基因表現 17 3.2.3 FoxA在澤蛭胚胎發育後期的基因表現 18 第四章 實驗討論 20 4.1 FoxA在胚層分化起源所扮演的角色 20 4.2 FoxA對於腸道發育過程中的角色 21 4.3 FoxA對於澤蛭胚胎神經索發育過程中的影響 22 參考文獻 24 圖 39 附錄 49 | |
dc.language.iso | zh-TW | |
dc.title | 探討澤蛭Helobdella胚胎發育過程中FoxA基因表現的時空分佈 | zh_TW |
dc.title | Expression patterns of FoxA genes during embryonic development of the leech Helobdella | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳俊宏(Jiun-Hong Chen),蔡素宜(Su-Yi Tsai) | |
dc.subject.keyword | 澤蛭,Forkhead box A,胚胎腸道發育,原位雜合技術, | zh_TW |
dc.subject.keyword | Leech embryonic development,Forkhead box A,in situ hybridization, | en |
dc.relation.page | 53 | |
dc.identifier.doi | 10.6342/NTU201602482 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-08-19 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生命科學系 | zh_TW |
顯示於系所單位: | 生命科學系 |
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