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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 薛景中 | |
| dc.contributor.author | Chen-Yi Wu | en |
| dc.contributor.author | 吳貞儀 | zh_TW |
| dc.date.accessioned | 2021-06-15T11:17:27Z | - |
| dc.date.available | 2018-08-26 | |
| dc.date.copyright | 2016-08-26 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-08-19 | |
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[94]http://www.sigmaaldrich.com/catalog/product/sigma/b6756?lang=enRion=TW. Sigma-Aldrich [95]http://www.sigmaaldrich.com/catalog/product/aldrich/861030?lang=enRion=TW. Sigma-Aldrich [96]http://www.sigmaaldrich.com/catalog/product/sigma/g5386. Sigma-Aldrich. [97]http://www.sigmaaldrich.com/catalog/product/sigma/p1319. Sigma-Aldrich. [98]http://www.phi.com/surface-analysis-techniques/tof-sims.html.Physical Electronics, Inc. 2003, 203, 449-452. [99] Time-of-flight secondary ion mass Spectrometry (TOF-SIMS) (2013) [100] A. Benninghoven, B. Hagenhoff, R. Kock, G. Assman and M. Walter In 5.4 Peptide Analysis by Time-of-flight Secondary Ion, Methods in Protein Sequence Analysis: Proceedings of the 7th International Conference, Berlin, July 3–8, 1988, Springer Science & Business Media: 2012; p 199. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49149 | - |
| dc.description.abstract | 二次離子質譜儀(SIMS)在表面分析上具有極高的靈敏度。加上近年來簇離子團(cluster ion)濺射技術的發展，二次離子質譜儀能夠產生高質量的離子破片，並分析未經同位素標記的混合樣品。而飛行式二次離子質譜儀(ToF-SIMS)屬於靜態二次離子質譜儀，在分析樣品後不會對其造成改變，因此也可對分析後的樣品加以回收。另外，利用高度聚焦的一次離子掃描樣品表面能獲得足夠的空間解析度，而且只需要少量的樣品消耗就能取得高解析度的分子分佈影像。但在離子源的濺射下，無法避免分子形成離子破片，造成質譜的複雜性，因此對於混合物的解析較為困難。濾紙層析為分離混合化學物質的一種分析方式。此外，通過使用紙基微流體分析裝置（μPADs），可設計對於物質檢測和分離之方便而便宜的分析平台。因此，對分子混合物的分析，可先以μPADs對混合物做大致的分離，再用TOF-SIMS得微流到影像，以解析分子。近來由於成本低廉與製備容易，紙質的微陣列是與SIMS分析整合的絕佳選擇。在本研究中，μPADs用改性玻璃微纖維紙製造。因為無機的玻璃纖維相較於一般的紙質濾紙在高質量區的質譜中有較低的背景質，較適合用來分析。製作微流道時，玻璃纖維的濾紙會經octadecyltrichlorosilane (OTS)自組裝單層膜(self-assemble monolayer)修飾成疏水性的表面，再使用雷射雕刻機選擇性的製作出親水性的微流道。接著在這些親水的微流道區域中分別滴入1 μL的胜肽(peptide)或色素混合水溶液，而尚未移除的OTS區域則用來作為疏水層。混合溶液在微流道內以不同的ph環境或極性溶液層析後，以脈衝20 kV C60+來獲得二次離子影像，解析混合溶液的分離。結合微流道與二次子質譜儀，其可呈現混合物在微流道內的分離。而分離後的混合物，可在二次離子質譜儀分析後，做進一步的分離。 | zh_TW |
| dc.description.abstract | Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) is a promising surface characterization technique that is able to address spectral analysis and direct imaging. With recent development of cluster ion beams, molecular species could also be examined according to their molecular weights without the need of labeling. High spatial resolution images of molecular distribution on a surface could also be acquired via using a focused primary ion scanning over the specimen. Besides, operated within the static regime, the specimen is nearly unaltered after analysis hence it is possible to recover the materials after analysis. However, with energetic ion bombardment, fragmentation of molecules is inevitable and results in complicate spectra. As a result, it is more difficult to identify components in a mixture. Paper chromatography is an analytical method that separates mixed chemicals or substances. In addition, with the use of microfluidic paper-based analytical devices (μPADs), a convenient and economical platform design for diagnostic assays can be achieved. Therefore, it is possible to preform rough separation of mixtures with μPADs and then image the microfluidic channel with ToF-SIMS to identify the components. In this work, the μPADs were fabricated with modified glass microfiber paper. The inorganic glass microfiber yield less background in the high mass regime than typical cellulose-based paper and do not interference with molecular analyze. The glass microfiber paper was modified with octadecyltrichlorosilane (OTS) to form a hydrophobic surface and then a laser scriber is used to selectively etch the OTS layer to form hydrophilic channels. 1μL aqueous drop of organic dye or peptides mixture was dripped onto the hydrophilic channels while the remaining OTS served as barrier. After preforming the chromatography under desired polarity or pH in the hydrophilic channel, secondary ion images were acquired with a focused C60+ pulse operated at 20 kV to image the distribution of separated molecules inside the microchannel. By combining μPADs and SIMS technique, the results revealed the separation of mixed compounds in μPADs and the composition of the mixture. The separated compounds could further be recovered after the SIMS identification. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T11:17:27Z (GMT). No. of bitstreams: 1 ntu-105-R03527023-1.pdf: 28521139 bytes, checksum: 664a7b1606bb7525853618c8d4a463a7 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 口試委員會審定書 I
致謝 II 中文摘要 III Abstract IV 目錄 V 第一章 緒論 1 第二章 文獻回顧 4 2.1 質譜儀於生物及有機分子之解析 4 2.2 二次離子質譜儀於生物及有機分子分析之應用 10 2.2.1 簇離子與多原子離子應用於二次離子質譜儀之演進 13 2.2.2 簇離子與多原子離子濺射機制 14 2.3 C60+離子源於二次離子質譜儀之應用 16 2.3.1 C 60+離子源濺射於SIMS中的優勢 — 二次離子產率提高 16 2.3.2 C 60+離子源濺射於SIMS中的優勢 — 低損傷機率 18 2.3.3 C 60+離子源濺射於SIMS中的優勢 — 有助影像建立於影像 19 2.4層析法 23 2.4.1蛋白質純化層析法 25 2.5微流體裝置 27 2.5.1微流體裝置之基材 30 2.5.2紙基微流體分析裝置(Microfluidic Paper-based Analytical Devices, μPADs) 33 2.5.3紙基微流道與分析裝置之製作 34 2.5.4紙基分析器及微流道裝置之應用 37 2.6自組裝單層膜(Self-Assemled Monolayer, SAM) 42 2.6.1 有機矽氧烷自組裝膜 45 2.7文獻回顧總結 49 第三章 實驗 50 3.1藥品與基材 50 3.2實驗儀器介紹 53 3.2.1飛行時間二次離子質譜儀(Time-of-Flight Secondary ion Spectrometer, ToF-SIMS ) 53 3.2.2掃描式電子顯微鏡(Scanning Electron Microscope, SEM) 55 3.2.3 CO2雷射雕刻機(CO2 laser scriber) 56 3.3 實驗步驟 57 3.3.1微流道製備 57 3.3.2樣品製備 58 3.3.3ToF-SIMS分析 58 3.3.4 SEM分析 60 第四章 實驗結果與討論 61 4.1微流道的製備 61 4.1.1 SEM 觀察 63 4.2混合樣品之分離 65 4.2.1色素混合溶液於不同條件下之分離 65 4.2.2胜肽混合溶液於不同條件下之分離 75 4.3樣品回收分析 83 第五章 結論 87 參考文獻 88 | |
| dc.language.iso | zh-TW | |
| dc.title | 以紙基微流道與分子型二次離子質譜術進行分子混合物之分離與檢測 | zh_TW |
| dc.title | Coupling of microfluidic paper-based separation devices and time-of-flight secondary ion mass spectroscopy (ToF-SIMS) for analyzing molecular mixtures | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 虞邦英,王榮輝 | |
| dc.subject.keyword | 飛行式二次離子質譜儀,紙基微流道,層析法,混合物分離, | zh_TW |
| dc.subject.keyword | ToF-SIMS,μPADs,chromatography,molecule separation, | en |
| dc.relation.page | 95 | |
| dc.identifier.doi | 10.6342/NTU201603013 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-08-20 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 材料科學與工程學研究所 | zh_TW |
| 顯示於系所單位： | 材料科學與工程學系 | |
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