請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48799完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 許富鈞(Fu-Chiun Hsu) | |
| dc.contributor.author | Bing-Yun Tsai | en |
| dc.contributor.author | 蔡秉芸 | zh_TW |
| dc.date.accessioned | 2021-06-15T11:09:31Z | - |
| dc.date.available | 2020-08-21 | |
| dc.date.copyright | 2020-08-21 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-08-18 | |
| dc.identifier.citation | 1. 中央氣象局. 2020. 氣象觀測年報表. 觀測資料查詢系統 CODiS. https://e-service.cwb.gov.tw/HistoryDataQuery/index.jsp 2. 王昭月、曾夢蛟. 2010. 利用葉綠素螢光與有效授粉估測甜椒之耐熱性. 台灣農業研究. 59 (4):237-248. 3. 陳葦玲、郭孚燿、陳榮五. 2009. 利用細胞膜熱穩定性技術篩選高耐熱性葉用蘿蔔. 臺中區農業改良場研究彙報. 102:15-29. 4. 苗琛、利容千、王建波. 1994. 熱脅迫下不結球白菜和甘藍葉片組織結構的變化. 武漢植物學研究. 12: 207-214. 5. Alché, J.D. 2019. A concise appraisal of lipid oxidation and lipoxidation in higher plants. Redox Biol. 23:101136. 6. Allakhverdiev, S.I., V.D. Kreslavski, V.V. Klimov, D.A. Los, R. Carpentier and P. Mohanty. 2008. Heat stress: an overview of molecular responses in photosynthesis. Photosyn. Res. 98:541. 7. Amooaghaie, R., and S. Moghym. 2011. Effect of polyamines on thermotolerance and membrane stability of soybean seedling. Afr. J. Biotechnol. 10(47):9677-9682. 8. Asada, K. 1996. Radical production and scavenging in the chloroplasts. p. 123-150. In: Photosynthesis and the environment. Springer, Dordrecht. 9. Ashraf, M. and M.R. Foolad. 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ. Exp. Bot. 59(2):206-216. 10. Bates, L.S., R.P. Waldren, and I.D. Teare, 1973. Rapid determination of free proline for water-stress studies. Plant Soil. 39(1):205-207. 11. Bhandari, K., K.D. Sharma, B.H. Rao, K.H. Siddique, P. Gaur, S.K. Agrawal, R.M. Nair, and H. Nayyar. 2017. Temperature sensitivity of food legumes: a physiological insight. Acta Physiol. Plant. 39(3):68. 12. Borgo, L., C.J. Marur, and L.G.E. Vieira. 2015. Effects of high proline accumulation on chloroplast and mitochondrial ultrastructure and on osmotic adjustment in tobacco plants. Acta Sci. Agron. 37(2):191-199. 13. Bukhov, N.G. and R. Carpentier. 2000. Heterogeneity of photosystem II reaction centers as influenced by heat treatment of barley leaves. Physiol. Plant. 110(2): 279-285. 14. Camejo, D., P. Rodríguez, M.A. Morales, J.M. Dell’Amico, A. Torrecillas, and J.J. Alarcón. 2005. High temperature effects on photosynthetic activity of two tomato cultivars with different heat susceptibility. J. plant physiol. 162(3):281-289. 15. Chavez-Arias, C. C., G. A. Ligarreto-Moreno, and H. Restrepo-Díaz. 2018. Evaluation of heat stress period duration and the interaction of daytime temperature and cultivar on common bean. Environ. Exp. Bot. 155:600-608. 16. Chen, W.L., W.J. Yang, H.F., Lo, and D.M. Yeh, 2014. Physiology, anatomy, and cell membrane thermostability selection of leafy radish (Raphanus sativus var. oleiformis Pers.) with different tolerance under heat stress. Sci. Hortic. 179:367-375. 17. Costa, E. S., R. Bressan-Smith, J. G. Oliveira E. Campostrini. 2003. Chlorophyll a fluorescence analysis in response to excitation irradiance in bean plants (Phaseolus vulgaris L. and Vigna unguiculata L. Walp) submitted to high temperature stress. Photosynthetica. 41(1):77-82. 18. Cook, B.G., B.C. Pengelly, S.D. Brown, J.L. Donnelly, D.A. Eagles, M.A. Franco, J. Hanson, B.F. Mullen, I.J. Partridge, M. Peters, and R. Schultze-Kraft. 2005. Tropical forages: an interactive selection tool. Lablab purpureus. CSIRO, DPI F(Qld), CIAT, and ILRI, Brisbane, Australia. http://www.tropicalforages.info/key/Forages/Media/Html/Lablab_purpureus.htm (accessed 24 May 2012). 19. Chen, H.H., Z.Y. Shen, and P.H. Li. 1982. Adaptability of crop plants to high temperatures stress. Crop Sci. 22(4):719-725. 20. Crafts-Brandner S.J. and M.E. Salvucci 2000. Rubisco activase constrains the photosynthetic potential of leaves at high temperature and CO2. Proc. Natl. Acad. Sci. USA. 97:13430-13435. 21. Djanaguiraman, M., P.V. Prasad, D.L. Boyle, and W.T. Schapaugh. 2011. High‐temperature stress and soybean leaves: Leaf anatomy and photosynthesis. Crop Sci. 51(5):2125-2131. 22. D’souza, M.R., and V.R. Devaraj. 2013. Induction of thermotolerance through heat acclimation in lablab bean (Dolichos lablab). Afr. J. Biotechnol. 12(38):5695-5704. 23. Flexas, J. and H. Medrano. 2002. Drought‐inhibition of photosynthesis in C3 plants: stomatal and non‐stomatal limitations revisited. Ann. Bot. 89(2): 183-189. 24. Forrester, M.L., G. Krotkov, and C.D. Nelson. 1966. Effect of oxygen on photosynthesis, photorespiration and respiration in detached leaves. Plant Physiol.41(3):422-427. 25. Fuller D.Q. 2003. African crops in prehistoric South Asia: A critical review. In: Neumann K, Butler EA, Kahlheber S (eds) Food, Fuel and Fields: Progress in African Archaeobotany. Africa Praehistorica 15. Heinrich-Barth-Institut, Köln, 239-271. 26. Genty, B., J.M. Briantais, and N.R. Baker. 1989. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim. Biophys. Acta. 990:87-92. 27. Gupta, N.K., S. Agarwal, V.P. Agarwal, N.S. Nathawat, S. Gupta, and G. Singh. 2013. Effect of short-term heat stress on growth, physiology and antioxidative defence system in wheat seedlings. Acta Physiol. Plant. 35(6):1837-1842. 28. Harsh, A., Y.K. Sharma, U. Joshi, S. Rampuria, G. Singh, S. Kumar, and R. Sharma. 2016. Effect of short-term heat stress on total sugars, proline and some antioxidant enzymes in moth bean (Vigna aconitifolia). Ann. Agri. Sci. 61(1):57-64. 29. Hameed, A. M. Goher, and N. Iqbal. 2012. Heat stress-induced cell death, changes in antioxidants, lipid peroxidation, and protease activity in wheat leaves. J. Plant Growth Regul. 31:283-291. 30. Heath, R.L. and L. Packer. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125:189-198. 31. Housley, T.L. and C.J. Pollock. 1985. Photosynthesis and carbohydrate metabolism in detached leaves of Lolium temulentum L. New Phytol. 99(4):499-507. 32. Hideg, E., P.B. Kos, and U. Schreiber. 2008. Imaging of NPQ and ROS formation in tobacco leaves: heat inactivation of the water–water cycle prevents down-regulation of PSII. Plant Cell Physiol. 49(12):1879-1886. 33. Hossain, M.M., H. Takeda, and T. Senboku. 1995. Proline content in Brassica under high temperature stress. JIRCAS J. Sci. Pap. (Japan). 2:87-93. 34. Hu, L., Z. Zhang, Z. Xiang, and Z. Yang. 2016. Exogenous application of citric acid ameliorates the adverse effect of heat stress in tall fescue (Lolium arundinaceum). Front. Plant Sci. 7:179. 35. Jifon, J.L. and J.P. Syvertsen, 2003. Moderate shade can increase net gas exchange and reduce photoinhibition in citrus leaves. Tree physiol. 23(2):119-127. 36. Karim, M.A., Y. Fracheboud, and P. Stamp. 2000. Effect of high temperature on seedling growth and photosynthesis of tropical maize genotypes. J. Agron. Crop Sci. 184(4):217-223. 37. Kaur, G., and B. Asthir. 2015. Proline: a key player in plant abiotic stress tolerance. Biol. Plant. 59(4):609-619. 38. Kaushal, N., K. Gupta, K. Bhandhari, S. Kumar, P. Thakur, and H. Nayyar. 2011. Proline induces heat tolerance in chickpea (Cicer arietinum L.) plants by protecting vital enzymes of carbon and antioxidative metabolism. Physiol. Mol. Biol. Plants. 17(3): 203. 39. Kautsky H. and A. Hirsch. 1934. Das Fluoreszenzverhalten grüner Pflanzen. Biochem. Z. 274: 422–434. 40. Kato, M. and S. Shimizu. 1987. Chlorophyll metabolism in higher plants. VⅡ. Chlorophyll degradation in senescing tobacco leaves; phenolic-dependent peroxidative degradation. Can. J. Bot. 65:729-735. 41. Law, R.D. and S.J. Crafts-Brandner. 1999. Inhibition and acclimation of photosynthesis to heat stress is closely correlated with activation of ribulose-1, 5-bisphosphate carboxylase/oxygenase. Plant physiol. 120(1):173-182. 42. Liu, X. and B. Huang. 2000. Heat stress injury in relation to membrane lipid peroxidation in creeping bentgrass. Crop Sci. 40(2):503-510. 43. Liu, J. and Y.S. Wang. 2020. Proline metabolism and molecular cloning of AmP5CS in the mangrove Avicennia marina under heat stress. Ecotoxicology. 29: 698-706. 44. Maass B.L., M.R. Knox, S.C. Venkatesha, T.A. Tefera, S. Ramme, and B.C. Pengelly. 2010. Lablab purpureus–a crop lost for Africa? Trop. Plant Biol. 3:123-135. 45. MacAdam, J.W., C.J. Nelson, and R.E. Sharp. 1992. Peroxidase activity in the leaf elongation zone of tall fescue: I. Spatial distribution of ionically bound peroxidase activity in genotypes differing in length of the elongation zone. Plant Physiol. 99(3):872-878. 46. Makonya, G.M., J.B. Ogola, A.M. Muasya, O. Crespo, S. Maseko, A.J. Valentine, C. Ottosen, E. Rosenqvist, and S. B. Chimphango. 2019. Chlorophyll fluorescence and carbohydrate concentration as field selection traits for heat tolerant chickpea genotypes. Plant Physiol. Biochem. 141:172-182. 47. Mittler R., S. Vanderauwera, M. Gollery, F. Van Breusegem. 2004. The reactive oxygen gene network in plants. Trends Plant Sci. 9: 490–498 48. Mugwira, L.M. and I. Haque 1993. Screening forage and browse legumes germplasm to nutrient stress: II. Tolerance of Lablab purpureus L. to acidity and low phosphorus in two acid soils. J. Plant Nutr. 16:37-50. 49. Nakano, Y. and K. Asada. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22:867-880. 50. Noreen, Z. and M. Ashraf. 2009. Changes in antioxidant enzymes and some key metabolites in some genetically diverse cultivars of radish (Raphanus sativus L.). Environ. Exp. Bot. 67(2):395-402. 51. Oukarroum, A., S. El Madidi, and R.J. Strasser. 2012. Exogenous glycine betaine and proline play a protective role in heat-stressed barley leaves (Hordeum vulgare L.): a chlorophyll a fluorescence study. Plant Biosyst. 146(4):1037-1043. 52. Pastenes, C., and P. Horton. 1999. Resistance of photosynthesis to high temperature in two bean varieties (Phaseolus vulgaris L.). Photosynth. Res. 62(2): 197-203. 53. Parys, E., E. Romanowska, M. Siedlecka, and J.W. Poskuta. 1998. The effect of lead on photosynthesis and respiration in detached leaves and in mesophyll protoplasts of Pisum sativum. Acta Physiol. Plant. 20(3):313-322. 54. Pengelly, B.C. and B.L. Maass. 2001. Lablab purpureus (L.) Sweet–diversity, potential use and determination of a core collection of this multi-purpose tropical legume. Genet. Resour. Crop Evol. 48(3):261-272. 55. Priya, M., L. Sharma, I. Singh, T.S. Bains, K.H. Siddique, H. Bindumadhava, R.M. Nair, and Nayyar, H. 2019. Securing reproductive function in mungbean grown under high temperature environment with exogenous application of proline. Plant Physiol. Biochem. 140:136-150. 56. Rai, N., K.K. Rai, G. Tiwari, and P.K. Singh. 2015. Changes in free radical generation, metabolites and antioxidant defense machinery in hyacinth bean (Lablab purpureus L) in response to high temperature stress. Acta Physiol. Plant. 37(3):46. 57. Rai, K.K., N. Rai, and S.P. Rai. 2018. Response of Lablab purpureus L. to high temperature stress and role of exogenous protectants in mitigating high temperature induced oxidative damages. Mol. Biol. Rep. 45(5):1375-1395. 58. Raison, J.K., J.A. Berry, P.A. Armond, and C.S. Pike. 1980. Membrane properties in relation to the adaptation of plants to temperature stress. p. 261-273. In: N.C. Turner, and P.J. Kramer. Adaptation of plants to water and high temperature stress. Wiley, New York. 59. Robotham, O. and M. Chapman. 2017. Population genetic analysis of hyacinth bean (Lablab purpureus (L.) Sweet, Leguminosae) indicates an East African origin and variation in drought tolerance. Genet. Resour. Crop Evol. 64(1):139-148. 60. Saadalla, M.M., J.S. Quick, and J.F. Shanahan. 1990. Heat tolerance in winter wheat: II. Membrane thermostability and field performance. Crop Sci. 30(6): 1248-1251. 61. Saed-Moucheshi, A., A. Shekoofa, and M. Pessarakli. 2014. Reactive oxygen species (ROS) generation and detoxifying in plants. J. Plant Nutr., 37(10):1573-1585. 62. Sennhenn, A., J.J.O. Odhiambo, B.L. Maass, and A.M. Whitbread. 2017. Considering effects of temperature and photoperiod on growth and development of Lablab purpureus (L.) Sweet in the search of short-season accessions for smallholder farming systems. Exp. Agri. 53(3):375-395. 63. Semenova, G. A. 2004. Structural reorganization of thylakoid systems in response to heat treatment. Photosynthetica. 42(2): 521-527. 64. Shaheen, M.R., C.M. Ayyub, M. Amjad, and E.A. Waraich. 2016. Morpho‐physiological evaluation of tomato genotypes under high temperature stress conditions. J Agric Food Sci. 96(8):2698-2704. 65. Sharma, D.K., J.O. Fernández, E. Rosenqvist, C.O. Ottosen, and S.B. Andersen. 2014. Genotypic response of detached leaves versus intact plants for chlorophyll fluorescence parameters under high temperature stress in wheat. J. Plant Physiol. 171(8):576-586. 66. Sharma, S. and P.E. Verslues. 2010. Mechanisms independent of abscisic acid (ABA) or proline feedback have a predominant role in transcriptional regulation of proline metabolism during low water potential and stress recovery. Plant Cell Environ. 33:1838-1851. 67. Sharkey, T.D. 2005. Effects of moderate heat stress on photosynthesis: importance of thylakoid reactions, rubisco deactivation, reactive oxygen species, and thermotolerance provided by isoprene. Plant Cell Environ. 28(3):269-277. 68. Singh, D., C.K. Singh, R.S. Singh Tomar, and M. Pal. 2017. Genetics and molecular mapping of heat tolerance for seedling survival and pod set in lentil. Crop Sci. 57(6): 3059-3067. 69. Sinsawat, V., J. Leipner, P. Stamp, and Y. Fracheboud. 2004. Effect of heat stress on the photosynthetic apparatus in maize (Zea mays L.) grown at control or high temperature. Environ. Exp. Bot. 52(2):123-129. 70. Stewart, C.R. and S.F. Boggess. 1978. Metabolism of 5-3H proline by barley leaves and its use in measuring the effects of water stress on proline oxidation. Plant Physiol. 61(4):654-657. 71. Takahashi, M.A. and K. Asada. 1983. Superoxide anion permeability of phospholipid membranes and chloroplast thylakoids. Arch. Biochem. Biophys. 226(2):558-566. 72. Tang, T., P. Liu, G. Zheng, and W. Li. 2016. Two phases of response to long-term moderate heat: Variation in thermotolerance between Arabidopsis thaliana and its relative Arabis paniculata. Phytochemistry. 122:81-90. 73. Sharkey, T.D. 2005. Effects of moderate heat stress on photosynthesis: importance of thylakoid reactions, rubisco deactivation, reactive oxygen species, and thermotolerance provided by isoprene. Plant Cell Environ. 28(3):269-277. 74. Tripathy, B.C. and R. Oelmüller. 2012. Reactive oxygen species generation and signaling in plants. Plant Signal Behav. 7(12):1621-1633. 75. Turner, W.B. and R.G.S. Bidwell. 1965. Rates of photosynthesis in attached and detached bean leaves, and the effect of spraying with indoleacetic acid solution. Plant Physiol. 40(3):446-451. 76. Wang, D., S.A. Heckathorn, K. Mainali, and R. Tripathee. 2016. Timing effects of heat-stress on plant ecophysiological characteristics and growth. Front. Plant Sci. 7: 1629. 77. Wahid, A., S. Gelani, M. Ashraf, and M.R. Foolad. 2007. Heat tolerance in plants: An overview. Environ. Expt. Bot. 61:199-223. 78. Weis, E. 1981. Reversible heat-inactivation of the calvin cycle: A possible mechanism of the temperature regulation of photosynthesis. Planta. 151:33-39. 79. Wu, M.T. and S.J. Wallner, 1983. Heat stress responses in cultured plant cells: development and comparison of viability tests. Plant Physiol. 72(3):817-820. 80. Xu, S., J. Li, X. Zhang, H. Wei, and L. Cui. 2006. Effects of heat acclimation pretreatment on changes of membrane lipid peroxidation, antioxidant metabolites, and ultrastructure of chloroplasts in two cool-season turfgrass species under heat stress. Environ. Expt. Bot. 56:274-285. 81. Yeh, D.M. and H.F. Lin. 2003. Thermostability of cell membranes as a measure of heat tolerance and relationship to flowering delay in chrysanthemum. J. Am. Soc. Horti. Sci. 128(5):656-660. 82. Yin H., Q. Chen, and M. Yi. 2008. Effects of short-term heat stress on oxidative damage and response of antioxidants system in Liltum longiflorum. Plant Growth Regul. 54:45-54. 83. Yu H.L., Z.Y. Fang, Y.M. Liu, L.M. Yang, M. Zhuang, H.H. Lv, Z.S. Li, F.Q. Han, X.P. Liu, and Y.Y. Zhang. 2016. Development of a novel allele-specific Rfo marker and creation of Ogura CMS fertility-restored interspecific hybrids in Brassica oleracea. Theor. Appl. Genet. 129:1625-1637. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48799 | - |
| dc.description.abstract | 鵲豆可用於蔬菜、雜糧、覆蓋作物等多重用途，同時對乾旱逆境耐受性高，在未來極端氣候中具有持續穩定供應糧食之潛力。為了確認鵲豆高溫下之生理反應，以應用於後續選育工作，本研究首先選出可於夏季花芽分化之日長不敏感鵲豆種原，接著調查所選出之農業試驗所9項種原於35°C輕度高溫中生長狀況，綜合評估株高、葉面積與乾重三項生長指標，選取L3、L19兩項為熱敏感品系及L12、L14兩項為耐熱品系，並測定生理反應指標。鵲豆苗期淨光合作用於35°C處理1天後效率皆提升，氣孔導度及蒸散作用提升指出鵲豆可能透過此路徑降低高溫傷害，光合作用系統二最大光效能(Fv/Fm)於熱敏感品系中未顯著變化，耐熱品系則於高溫後Fv/Fm輕微下降、非光化學淬熄係數(qN)上升，顯示光合作用系統二有受損情形，並透過熱能逸散出多餘能量。高溫後耐熱品系L12及L14丙二醛含量累積量較低，顯示氧化逆境在耐熱品系中較為輕微，抗氧化酵素抗壞血酸過氧化酶及過氧化氫酶於高溫處理後活性皆被誘導，而過氧化酶活性及脯胺酸含量高溫後皆未顯著變化。為建立快速評估耐熱性之方法，利用離體葉片進30°C至60°C高溫處理，觀察細胞膜熱穩定度與葉綠素螢光表現之變化，並比對半致死溫度與生長指標變化量之相關性，葉綠素螢光表現與各項生長指標均屬於低度相關，細胞膜熱穩定度之半致死溫度與葉面積變化具中等相關性。結果顯示丙二醛與離體葉片之細胞膜熱穩定度表現，與耐熱性相關性較高，可做為未來耐熱選拔之指標。 | zh_TW |
| dc.description.abstract | Lablab bean is a multipurpose crop, that is used as vegetable, pulse and covered crop. Apart from various uses, lablab bean has great tolerance to drought, having potential to stabilize food production under extreme climate. In this study, light period insensitive accessions which is capable to differentiate flower bud under summer were selected. Growth of nine selected accessions from Taiwan Agricultural Research Institute were observed under moderate high temperature to understand the physiological response of lablab bean under heat stress and apply to breeding program. Comprehensive evaluation of three growth indicators, plant height, leaf area and dry weight were conducted. We selected L3 and L19 as heat susceptible accessions, and L12 and L14 as heat tolerant accessions. After 35°C treatment for 1 day, net assimilation rate was induced. At the same time, stomatal conductance and transpiration rate raised, indicating lablab bean could alleviate heat damage by transpiration. Maximal quantum efficiency of PSII photochemistry (Fv/Fm) did not change in heat susceptible accessions. However, Fv/Fm of heat tolerant accessions slightly decreased and non-photochemical quenching coefficient (qN) increased, indicating that PSII could be damaged and excessive energy was dissipated thermally. Under heat stress, L12 and L14 accumulated less malondialdehyde (MDA), suggesting that heat tolerant accessions have less oxidative pressure. Antioxidant enzymes ascorbate peroxidase (APX) and catalase (CAT) were induced after heat treatment. However, peroxidase (POD) activity and proline content remained at the same level. In order to establish an index to screen heat tolerance rapidly, we determined the correlation between semi-lethal temperature and growth parameters, using detached leaves to give heat treatment from 30°C to 60°C. Correlation between chlorophyll fluorescence and growth parameters were low. The correlation between cell membrane thermostability (CMS) and plant height were medium level. In conclusion, MDA content and CMS of detached leaves have higher correlation with heat tolerance, indicating that MDA and CMS can apply to heat tolerance selection in the future. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T11:09:31Z (GMT). No. of bitstreams: 1 U0001-1308202011404600.pdf: 2001467 bytes, checksum: a44093869d34c84ce0e4c7f8544a32cf (MD5) Previous issue date: 2020 | en |
| dc.description.tableofcontents | 目錄 致謝....................................................I 摘要...................................................II Abstract..............................................III 圖目錄................................................VIII 表目錄..................................................IX 一、前言.................................................1 二、前人研究..............................................3 (一)鵲豆生育特性.........................................3 (二)高溫對光合作用之影響.................................3 (三)高溫對細胞膜穩定性之影響..............................4 (四)植物耐熱性形成.......................................5 1.活性氧化物與過氧化物形成..............................5 2.抗氧化酵素活性......................................6 3.脯胺酸累積..........................................7 (五)離體葉片之生理反應..................................8 三、材料與方法..........................................9 (一)植物材料與栽培處理..................................9 (二)試驗設計與統計分析.................................9 (三)高溫下鵲豆植株發育狀況.............................9 1.生長發育狀況.......................................9 2.乾重測定..........................................10 3.株高測定..........................................10 4.葉面積測量........................................11 (四)高溫下鵲豆植株生理變化............................10 1.植株栽培及採樣....................................10 2.葉綠素螢光分析....................................10 3.氣體交換.........................................11 4.丙二醛含量分析....................................11 5.抗氧化酵素分析....................................11 6.脯胺酸含量分析....................................13 (五)以鵲豆離體葉片生理反應評估植株耐熱性...............13 1.細胞膜穩定度測定..................................13 2.離體葉片葉綠素螢光表現.............................14 四、 結果.............................................15 (一)高溫下鵲豆植株發育狀況............................15 (二)高溫下鵲豆植株生理變化............................19 1.氣體交換..........................................19 2.葉綠素螢光表現.....................................19 3.活性氧化物及過氧化物累積............................20 4.抗氧化酵素活性.....................................20 5.脯胺酸累積.........................................21 (三)以鵲豆離體葉片生理反應評估植株耐熱性................28 1.細胞膜熱穩定度.....................................28 2.葉綠素螢光表現.....................................28 五、 討論.............................................34 (一)高溫下鵲豆植株發育狀況.............................34 (二)高溫下鵲豆植株生理變化.............................35 1.光合作用效率變化...................................35 2.活性氧化物清除能力及滲透調節物質累積.................36 (三)以細胞膜熱穩定度及葉綠素螢光初步評估植株耐熱性.......37 六、結論及未來研究方向..................................39 參考文獻...............................................41 | |
| dc.language.iso | zh-TW | |
| dc.subject | 細胞膜熱穩定度 | zh_TW |
| dc.subject | 葉綠素螢光 | zh_TW |
| dc.subject | 蒸散作用 | zh_TW |
| dc.subject | 氣孔導度 | zh_TW |
| dc.subject | 抗氧化酵素 | zh_TW |
| dc.subject | 脯胺酸 | zh_TW |
| dc.subject | 離體葉片 | zh_TW |
| dc.subject | antioxidant enzyme | en |
| dc.subject | cell membrane thermostability | en |
| dc.subject | detached leaf | en |
| dc.subject | proline | en |
| dc.subject | chlorophyll fluorescence | en |
| dc.subject | transpiration rate | en |
| dc.subject | stomatal conductance | en |
| dc.title | 鵲豆在輕度高溫下之生理反應及耐熱指標 | zh_TW |
| dc.title | Physiological Response and High Temperature Index of Lablab purpureus under Moderate High Temperature | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李國譚(Kuo-Tan Li),官彥州(Yen-Chou Kuan) | |
| dc.subject.keyword | 葉綠素螢光,蒸散作用,氣孔導度,抗氧化酵素,脯胺酸,離體葉片,細胞膜熱穩定度, | zh_TW |
| dc.subject.keyword | chlorophyll fluorescence,transpiration rate,stomatal conductance,antioxidant enzyme,proline,detached leaf,cell membrane thermostability, | en |
| dc.relation.page | 52 | |
| dc.identifier.doi | 10.6342/NTU202003212 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2020-08-19 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 園藝暨景觀學系 | zh_TW |
| 顯示於系所單位： | 園藝暨景觀學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| U0001-1308202011404600.pdf 未授權公開取用 | 1.95 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。