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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 陳靜宜 | |
dc.contributor.author | Chun-Hsien Chiang | en |
dc.contributor.author | 江俊嫻 | zh_TW |
dc.date.accessioned | 2021-06-15T06:54:35Z | - |
dc.date.available | 2014-02-20 | |
dc.date.copyright | 2011-02-20 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-02-11 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48380 | - |
dc.description.abstract | 當細胞內產生過多的活性氧(ROS)使細胞內抗氧化系統無法清除而達到平衡時便會形成氧化壓力,過度之氧化壓力可導致細胞走向死亡。其死亡方式主要可分為三種,細胞壞死及兩種型態之計畫性細胞死亡,第一型之細胞凋亡以及第二型之細胞自噬,許多心臟疾病中皆可觀察到嚴重之氧化壓力,如心臟衰竭。
EPA 為魚油之主要成分之一,屬於n-3 多不飽和脂肪酸。魚油的功能非常多,其具有抗發炎反應之能力,同時也可降低心臟疾病之死亡率。雖然魚油具有治療心臟疾病之潛力,然而至目前為止,其作用機制尚不清楚,因此本實驗主要研究的方向為探討心肌細胞於不同程度之氧化壓力下之細胞死亡型態以及釐清EPA 保護心肌細胞減少細胞遭受氧化壓力之破壞機制。 本試驗之第一個部分為測試不同程度氧化壓力(100,μ 200, 400μM H2O2)所引發之細胞死亡種類,而其結果顯示,細胞存活率會隨著氧化濃度之上升而隨之下降,而在400μM H2O2 之情況下若添加細胞自噬抑制劑,3-MA,以抑制細胞自噬之起始,則會降低細胞存活率。於蛋白質部分,在氧化壓力之情況下則可見細胞內之LC 3 II/I 之比例上升以及cathepsin D 之降低,綜合上述可知,氧化壓力造成之細胞死亡可能有部份藉由細胞自噬所調控。而本實驗之第二部分為探討EPA 之添加對保護細胞抵抗嚴重氧化壓力之效果,結果顯示,EPA 確實可以保護細胞,減少細胞因氧化壓力所造成之傷害,但若抑制細胞自噬之起始,會使EPA 之保護能力效果降低,增加細胞之死亡率。同時於蛋白質的表現中也可發現,魚油之添加,可提高氧化壓力中Beclin 1、cathepsin D 之表現量、降低LC 3 II/I 之比例,同時也發現,魚油添加可提高氧化壓力下細胞自噬之進行。而後為更進一步了解其作用機制,因此使用AMPK 抑制劑,AMPK 為細胞自噬上游路徑之一,結果發現,其對蛋白質及存活率皆無調控效應。綜合上述之結果可知,EPA 之添加可保護細胞,降低氧化壓力對細胞所造成之傷害,而其保護作用有部分來自於促進細胞自噬之起始,但其上游並非透過AMPK 之路徑,其詳細之作用機制,尚需更進一步的研究。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-15T06:54:35Z (GMT). No. of bitstreams: 1 ntu-100-R97626014-1.pdf: 4657545 bytes, checksum: 0de7dfec46258294237dc96a1c0bfef8 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 目錄 I
圖目錄 IV 表目錄 V 中文摘要 VI 英文摘要 VIII 壹、前言 1 貳、文獻回顧 3 2.1氧化壓力 3 2.1.1活性氧之生成 3 2.1.2氧化壓力與心臟疾病 4 2.2 細胞死亡方式 5 2.2.1細胞凋亡 5 2.2.2細胞自噬 6 2.2.3細胞壞死 7 2.2.4溶小體與細胞死亡之關係 9 2.3細胞死亡與心臟 10 2.3.1心臟 10 2.3.2.心臟與細胞死亡 11 2.3.2.1細胞凋亡與心臟疾病 11 2.3.2.2細胞自噬與心臟疾病 12 2.4二十碳五烯酸 13 2.4.1魚油 13 2.4.2二十碳五烯酸之合成 13 2.4.3二十碳五烯酸之功能與代謝 14 2.4.4魚油對心臟疾病之探討 14 参、材料與方法 16 3.1細胞培養 16 3.2過氧化氫 ( Hydrogen peroxide , H2O2 )配製方法 16 3.3 EPA配製及處理方法 16 3.4細胞存活率 16 3.5蛋白質萃取 17 3.6西方點墨法 (western blot) 17 3.7細胞凋亡之測定 19 3.8酸性胞囊之測定 19 3.8.1共軛焦顯微鏡 19 3.8.2流式細胞儀 20 3.9統計分析 20 肆、試驗結果 21 4.1氧化壓力下H9c2細胞之死亡型態 21 4.1.1適當的氧化壓力處理時間 21 4.1.2不同氧化壓力下之細胞死亡型態 21 4.1.3不同氧化壓力下與細胞死亡型態相關之蛋白質表現 22 4.2 EPA於氧化壓力下所扮演之角色 23 4.2.1 EPA對細胞於氧化壓力下之作用 23 4.2.2 EPA處理與細胞之死亡型態 23 4.2.3 EPA處理下細胞自噬相關蛋白質之表現量 23 4.2.4 EPA處理可提高氧化壓力下溶小體之數量 24 4.2.5 EPA處理與AMPK調控細胞自噬之關聯性 24 伍、討論 25 5.1不同程度之氧化壓力對細胞死亡之影響 25 5.1.1細胞自噬之起始於不同程度氧化壓力下所扮演之角色 25 5.1.2 Bafilomycin A在不同程度氧化壓力下所扮演之角色 26 5.2 EPA改善氧化壓力 26 5.2.1細胞自噬起始所扮演之角色 27 陸、結論 28 參考文獻 29 圖 38 | |
dc.language.iso | zh-TW | |
dc.title | 二十碳五烯酸保護氧化催迫下心肌細胞之能力 | zh_TW |
dc.title | Eicosapentaenoic acid has protective effects against oxidative stress in cardiomyocytes | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 許秀卿,蔡淑慧,丁詩同 | |
dc.subject.keyword | EPA,細胞自噬,氧化壓力,計畫性細胞死亡,心臟, | zh_TW |
dc.subject.keyword | EPA,Autophagy,oxidative stress,programmed cell death,heart, | en |
dc.relation.page | 48 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-02-11 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 動物科學技術學研究所 | zh_TW |
顯示於系所單位: | 動物科學技術學系 |
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