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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王亞男 | |
dc.contributor.author | Hong-Yu Shy | en |
dc.contributor.author | 許弘諭 | zh_TW |
dc.date.accessioned | 2021-06-15T06:49:26Z | - |
dc.date.available | 2011-03-12 | |
dc.date.copyright | 2011-03-12 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-03-07 | |
dc.identifier.citation | 古森本。2008。生質能源作物之開發與潛力。植物種苗生技 13:46-53。
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48225 | - |
dc.description.abstract | 華山松種子具有休眠性,若在發芽前將華山松種子處以10週的5℃低溫層積處理,則種子發芽率將從原本的16%增加至20%。此外,在不同發芽溫度試驗中發現華山松種子最適合的發芽環境是在10℃/20℃的變溫條件下,其發芽率明顯優於20℃/30℃與15℃/25℃。
對於層積處理可以提高種子發芽力的現象,利用GC-MS-SIM對經過2、4、6週低溫層積處理的不同階段華山松種子胚部做ABA(Abscisic acid)GAs (Gibberellins)化學物質研究,發現這段期間內激素的變化量以ABA最為明顯,ABA濃度在低溫層積處理初期較高,每1g乾重的華山松胚部細胞中有250ng的ABA,但是在經過4週低溫層積處理的樣品中則發現ABA有減少的趨勢,濃度降至1g乾物重中有約150ng左右的,濃度減少了40%,直至6週之後濃度都維持在1g乾重胚部細胞中有150ng的ABA含量。推測低溫層積處理對於華山松體內ABA濃度的影響是將其濃度降低至一定值。 GAs是影響種子發芽的激素,在2、4、6週低溫層積處理期的不同階段中發現,在第2週時所偵測到的種類以GA4為最大宗,其後濃度變化漸減,而在第6週時偵測到GA1濃度大量增加,顯示在低溫層積處理期間,種子內部已經開始產生具有促進發芽的GA種類。 將實驗所得數據比照GA生合成路徑,non-13-hydroxylation pathway與 early 13-hydroxylation pathway兩種,因為實驗中發現到GA4、GA24以及在第6週的樣本中發現大量的GA1,對照GA生合成路徑圖可發現在低溫層積處理期間,華山松種子體內有進行non-13-hydroxylation pathway的GA生合成路徑。 | zh_TW |
dc.description.abstract | Seeds that unable to germinate is a serious problem when people try to breeding seedlings. Pinus armandii ver. mastersiana has low germination percentage and dormancy. Treating its seeds with cold stratification for ten weeks could broke seed dormancy and increase seed germination percentage from 12.5% to 15%. Considered with the viability of seed is 20%. Make this result as 100%. The germination percentage increased from 62.5% to 75%.
Using GC-MS-SIM to detect concentrations of ABA(abscisic acid) and GAs(gibberellins) in P. armandii seed embryos by two weeks, four weeks and six weeks cold stratification. Results showed two weeks cold-stratified had ABA concentration of 262ng per gram dry weight. ABA concentration decreases to 179 ng per gram dry weight at cold-stratified for four weeks. It was 31% reduction. At the cold-stratified for six weeks, the concentration of ABA was 155 ng per gram dry weigh. The decrease of ABA concentration could be one of the reasons that seed germination increase after cold stratification. The changes of GAS concentrations were great found in P. armandii seed embryos. The concentration of GA3 was detected after cold stratification for two weeks which was 37.5 ng per gram dry weigh. But it disappeared at samples which after four weeks cold stratification, in which GA3 replaced with GA1 to be the main GAS. GA4 and GA24 were also detected in the sample which after four weeks cold stratification. GA1 was detected at the cold-stratified for six weeks embryos. Results showed GA syntheses pathway in the embryo was proceed by non-13-hydroxylation pathway during cold stratification. This syntheses pathway is lack of function to produce GA1. Cold stratification to P. armandii seed embryos was increase the ability to produce GA1. Seed dormancy is a complicate mechanism for plants. By studying different kinks ofplants, tissues and germination stage can help us to realize the main factors of seed dormancy. At the feature, the study of breaking seed dormancy could enhance seed germination and increase the amount of seedlings. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T06:49:26Z (GMT). No. of bitstreams: 1 ntu-100-R96625030-1.pdf: 1574231 bytes, checksum: 809b32765bed242ab971a16aef682f3e (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 口試委員會審定書…………………………………………………………………… i
誌謝…………………………………………………………………………………… ii 中文摘要………………………………………………………………………………iii 英文摘要………………………………………………………………………………iv 第一章 前言…………………………………………………………………………1 第二章 前人研究……………………………………………………………………5 2.1 種子休眠相關研究……………………………………………………5 2.2 種子發芽相關研究……………………………………………………6 2.3 華山松…………………………………………………………………7 2.4 種子發芽與休眠相關之激素研究……………………………………9 2.4.1 GA相關研究……………………………………………………10 2.4.2 ABA相關研究…………………………………………………14 2.4.3 GA與ABA的拮抗作用…………………………………………15 第三章 材料與方法………………………………………………………………16 3.1 種子材料………………………………………………………………16 3.2 最適發芽變溫環境試驗方法…………………………………………17 3.3 5℃冷溫層積處理後種子發芽試驗…………………………………17 3.4 TTC種子活力檢查方法………………………………………………18 3.5 不同冷溫層積處理階段華山種子胚部GA與ABA之化學分析……18 3.5.1 GA與ABA之化學分析方法與步驟…………………………19 3.5.2 實驗儀器………………………………………………………23 3.6 種子發芽率與發芽速率(平均發芽天數)計算…………………24 3.7 統計分析法……………………………………………………………24 第四章 結果………………………………………………………………………23 4.1 5℃冷溫層積處理後種子胚部外觀之變化…………………………23 4.2 GA濃度及種類………………………………………………………27 4.3 ABA濃度………………………………………………………………29 4.4 種子發芽試驗…………………………………………………………30 4.4.1 最適發芽變溫環境試驗…………………………………………30 4.4.2 5℃冷溫層積處理後種子發芽試驗……………………………32 4.5 不正常發芽……………………………………………………………33 4.6 TTC種子活力檢查……………………………………………………34 第五章 討論………………………………………………………………………35 5.1 種子發芽試驗…………………………………………………………35 5.2 最適發芽環境試驗……………………………………………………35 5.3 層積處理後種子發芽驗………………………………………………37 5.4 TTC種子活力檢測………………………………………………39 5.5 GA與ABA化學分析………………………………………………39 5.5.1 GA種類及濃度分析試驗……………………………………39 5.5.2 ABA濃度分析試驗………………………………………………41 第六章 結論………………………………………………………………………44 引用文獻……………………………………………………………………………45 圖目錄 圖一GA主要結構…………………………………………………………11 圖二 GA生合成路徑圖……………………………………………………13 圖三ABA結構………………………………………………………………14 圖四 華山松種子…………………………………………………………16 圖五 華山松……………………………………………………………16 圖六 層積處理2週之華山松種子胚部…………………………………25 圖七 層積處理4週之華山松種子胚部…………………………………26 圖八 層積處理6週之華山松種子胚部…………………………………26 圖九 5℃冷溫層積處理不同階段GA種類及濃度變化…………………28 圖十不同冷溫層積處理階段ABA濃度變化……………………………29 圖十一各不同處理之平均種子發芽率………………………………32 圖十二 華山松種子不正常發芽…………………………………………33 圖十三 華山松種子不正常發芽…………………………………………33 圖十四 華山松種子TTC活力檢查………………………………………35 表目錄 表一冷溫層積處理不同階段之華山松胚部GA種類及濃度變化………………27 表二不同發芽環境與冷溫層積處理對華山松種子發芽之影響…………………31 表三不同發芽環境與冷溫層積處理之平均發芽率與平均發芽天數……………31 | |
dc.language.iso | zh-TW | |
dc.title | 華山松種子發芽與不同階段之低溫層積處理改變華山松種子胚部激勃素和離層酸濃度之研究 | zh_TW |
dc.title | Changes of gibberellins and abscisic acid levels in embryos of Pinus armandii var. mastersiana seeds during different stages of cold stratified treatment | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李明仁,簡慶德,廖天賜,蕭英倫 | |
dc.subject.keyword | GA,ABA,冷溫層積處理,華山松,種子休眠, | zh_TW |
dc.subject.keyword | GA(Gibberellins),ABA (Abscisic acid),cold stratification,Pinus armandii,seed germination,seed dormancy, | en |
dc.relation.page | 52 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-03-09 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
顯示於系所單位: | 森林環境暨資源學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
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ntu-100-1.pdf 目前未授權公開取用 | 1.54 MB | Adobe PDF |
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