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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48061完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 董成淵(Dong-Chen Yuan) | |
| dc.contributor.author | Halley-F. Tsai | en |
| dc.contributor.author | 蔡秉儒 | zh_TW |
| dc.date.accessioned | 2021-06-15T06:45:22Z | - |
| dc.date.available | 2011-07-25 | |
| dc.date.copyright | 2011-07-25 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-06-27 | |
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scopist’s perspective,” Micron, vol. 32, pp. 261–272, April 2001. [2] D. Maurice, “The structure and transparency of the cornea,” J Physiol., vol. 136, pp. 263–286, April 1957. [3] J. A. Chapman, M. Tzaphlidou, K. M. Meek, and K. E. Kadler, “The collagen fibril - a model system for studying the staining and fixation of a protein.,” Electron Microsc Rev., vol. 3, no. 1, pp. 143–182, 1990. [4] Y. Komai and T. Ushiki, “The three-dimensional organization of collagen fibrils in the human cornea and sclera,” Invest. Ophthalmol. Vis. Sci., vol. 32, pp. 2244–2258, July 1991. [5] W. Radner, M. Zehetmayer, R. Aufreiter, and R. Mallinger, “Interlacing and cross-angle distribution of collagen lamellae in the human cornea.,” Cornea, vol. 17, pp. 537–543, September 1998. [6] K. M. Meek and C. Boote, “The organization of collagen in the corneal stroma,” Experimental Eye Research, vol. 78, pp. 503–512, July 2004. [7] C. Boote, S. Dennis, R. H. Newton, H. Puri, and K. M. Meek, “Collagen 63 fibrils appear more closely packed in the prepupillary cornea,” Invest. Oph- thalmol. Vis. Sci., vol. 44, pp. 2941–2948, July 2003. [8] M. Abahussin, S. Hayes, N. E. K. Cartwright, C. S. Kamma-Lorger, Y. Khan, J. Marshall, and K. M. Meek, “3d collagen orientation study of the human cornea using x-ray diffraction and femtosecond laser technology,” Invest. Ophthalmol. Vis. Sci, vol. 50, pp. 5159–5164, November 2009. [9] J. V. Jester, M. Winkler, B. E. Jester, C. Nien, D. Chai, and D. J. Brown, “Evaluating corneal collagen organization using high-resolution nonlinear optical macroscopy,” Eye & Contact Lens: Science & Clinical Practice, vol. 36, pp. 260–264, September 2010. [10] C. Bayan, J. M. Levitt, E. Miller, D. Kaplan, and I. Georgakoudi, “Fully automated, quantitative, noninvasive assessment of collagen fiber content and organization in thick collagen gels,” Journal of Applied Physics, vol. 105, p. 102042, May 2009. [11] R. A. Rao, M. R. Mehta, and K. C. Toussaint, “Fourier transform-second- harmonic generation imaging of biological tissues,” Optics Express, vol. 17, pp. 14534–14542, August 2009. [12] M. Sivaguru, S. Durgam, R. Ambekar, D. Luedtke, G. Fried, A. Stewart, and K. C. Toussaint, “Quantitative analysis of collagen fiber organization in in- jured tendons using fourier transform-second harmonic generation imaging,” Optics Express, vol. 18, pp. 24983–24993, November 2010. [13] P. Matteini, F. Ratto, F. Rossi, R. Cicchi, C. Stringari, D. Kapsokalyvas, F. S. Pavone, and R. Pini, “Photothermally-induced disordered patterns of corneal 64 collagen revealed by shg imaging,” Optics Express, vol. 17, pp. 4868–4878, March 2009. [14] S. Wu, H. Li, H. Yang, X. Zhang, Z. Li, and S. Xu, “Quantitative analysis on collagen morphology in aging skin based on multiphoton microscopy,” Journal of Biomedical Optics Fourier, vol. 16, p. 040502, April 2011. [15] M. R. Tsai, Y. W. Chiu, M. T. Lo, and C. K. Sun, “Second-harmonic gener- ation imaging of collagen fibers in myocardium for atrial fibrillation diagno- sis,” Journal of Biomedical Optics, vol. 15, p. 026002, March 2010. [16] R. H. Newton and K. M. Meek, “Circumcorneal annulus of collagen fibrils in the human limbus,” Invest. Ophthalmol. Vis. Sci., vol. 39, pp. 1125–1134, June 1998. [17] M. Han, G. Giese, and J. Bille, “Second harmonic generation imaging of collagen fibrils in cornea and sclera,” Optics Express, vol. 13, pp. 5791–5797, July 2005. [18] R. Shankar, Principles of Quantum Mechanics. Plenum Press, New York, second ed., 1994. [19] J. Sakurai, Modern Quantum Physics. Addison-Wesley Publishing Company, revised ed., 1994. [20] R. Boyd, Nonlinear Optics. Academic Press, third ed., 2003. [21] E. Candes, J. Romberg, and T. Tao, “Stable signal recovery from incomplete and inaccurate measurements,” Communications on Pure and Applied Math- ematics, vol. 59, pp. 1207–1223, August 2006. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48061 | - |
| dc.description.abstract | 動物體內有不少組織以膠原蛋白纖維所構成，其中以眼角膜、肌腱和真皮最為廣泛地討論。三者之間就結構複雜程度而言，又以眼角膜為最，它獨特的光學特性和排列結構吸引了許多膠原蛋白纖維研究者的目光。最初從 X 光繞射並觀察繞射圖樣逆推纖維原始的樣貌，近兩三年來開始有人以傅立葉分析取代傳統的 X 光繞射，並提出強度分析和橢圓擬合等方法。本研究補充了過去對傅立葉二倍頻顯微術的不足，著重於提出一個重覆能力高，客觀且一般化的分析方式，首次針對感興趣的區域 (Domain) 進行系統化的探究，並發現隨著 ROI 尺寸的不同，對於樣本分析結果有十分顯著的影響。擬合橢圓的扁平率 e 隨著 Domain 尺寸的變化反映出樣本的均質性 (Homogeneous) 和中心對稱的程度 (Isotropic)。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-15T06:45:22Z (GMT). No. of bitstreams: 1 ntu-100-R97222018-1.pdf: 16233945 bytes, checksum: 2d2c38c9aa06c19fd0e8630478ad43e8 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
摘要 iii 1 前言 1 1.1 研究動機.............................. 1 1.2 研究簡介.............................. 4 2 實驗原理 7 2.1 光學解析度............................. 7 2.2 螢光................................. 11 2.2.1 單光子激發 ........................ 15 2.2.2 雙光子激發 ........................ 17 2.3 二倍頻輻射............................. 20 2.3.1 偶合波動方程式與頻率疊加效應 ............ 21 2.3.2 相位匹配.......................... 24 2.4 二倍頻物質............................. 26 2.5 傅立葉轉換............................. 27 3 實驗方法 31 3.1 光學系統.............................. 31 3.2 自動化控制系統 .......................... 33 3.3 分析方法.............................. 34 3.3.1 取樣原則.......................... 34 3.3.2 傅立葉轉換 ........................ 35 3.3.3 閥值 ............................ 37 3.3.4 侵蝕與擴張 ........................ 37 3.3.5 橢圓擬合.......................... 38 3.4 不準度 ............................... 43 3.4.1 小區域下的數值誤差 ................... 43 3.4.2 距離標定與不準度 .................... 44 4 研究結果 47 4.1 扁平率AspectRatio(AR)隨Domain的變化 .................... 47 4.2 角度與AR的交叉分析 ...................... 53 5 結論 61 文 獻 63 | |
| dc.language.iso | zh-TW | |
| dc.title | 以傅立葉二倍頻顯微術分析膠原蛋白纖維的區塊特徵 | zh_TW |
| dc.title | Analysis of Collagen Fiber Domain Organization by Fourier-SHG Microscopy | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 譚欣媛(Shin-Yuan Tan),石明豐(Ming-Fung Shih),楊鴻昌(Hong-Chang Yang) | |
| dc.subject.keyword | 二倍頻,顯微術,纖維,傅立葉,膠原蛋白, | zh_TW |
| dc.subject.keyword | SHG,Microscopy,Collagen,Fiber,Fourier, | en |
| dc.relation.page | 65 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-06-28 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 物理研究所 | zh_TW |
| 顯示於系所單位： | 物理學系 | |
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| ntu-100-1.pdf 目前未授權公開取用 | 15.85 MB | Adobe PDF |
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