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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 施明哲(Ming-Che Shih) | |
dc.contributor.author | Wei-Chu Su | en |
dc.contributor.author | 蘇煒筑 | zh_TW |
dc.date.accessioned | 2021-06-15T06:45:01Z | - |
dc.date.available | 2012-07-07 | |
dc.date.copyright | 2011-07-07 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-06-28 | |
dc.identifier.citation | Amor, Y., Haigler, C.H., Johnson, S., Wainscott, M., and Delmer, D.P. (1995). A membrane-associated form of sucrose synthase and its potential role in synthesis of cellulose and callose in plants. Proc Natl Acad Sci U S A 92: 9353-9357.
Anguenot, R., Nguyen-Quoc, B., Yelle, S., and Michaud, D. (2006). Protein phosphorylation and membrane association of sucrose synthase in developing tomato fruit. Plant Physiol Biochem. 44: 294-300. Armstrong, W. (1980). Aeration in higher plants. Advances in botanical research 7: 225-332. Babb, V.M., and Haigler, C.H. (2001). Sucrose phosphate synthase activity rises in correlation with high-rate cellulose synthesis in three heterotrophic systems. Plant Physiol. 127: 1234-1242. Bailey-Serres, J., and Voesenek, L.A. (2008). Flooding stress: acclimations and genetic diversity. Annu Rev Plant Biol. 59: 313-339. Baud, S., Vaultier, M.N., and Rochat, C. (2004). Structure and expression profile of the sucrose synthase multigene family in Arabidopsis. J Exp Bot. 55: 397-409. Bieniawska, Z., Paul Barratt, D.H., Garlick, A.P., Thole, V., Kruger, N.J., Martin, C., Zrenner, R., and Smith, A.M. (2007). Analysis of the sucrose synthase gene family in Arabidopsis. Plant J. 49: 810-828. Blom, C.W., and Voesenek, L.A. (1996). Flooding: the survival strategies of plants. Trends Ecol Evol. 11: 290-295. Branco-Price, C., Kawaguchi, R., Ferreira, R.B., and Bailey-Serres, J. (2005). Genome-wide analysis of transcript abundance and translation in arabidopsis seedlings subjected to oxygen deprivation. Ann Bot-London. 96: 1142-1142. Buckeridge, M.S., Vergara, C.E., and Carpita, N.C. (1999). The mechanism of synthesis of a mixed-linkage (1-->3), (1-->4)beta-D-glucan in maize. Evidence for multiple sites of glucosyl transfer in the synthase complex. Plant Physiol. 120: 1105-1116. Cai, G., Faleri, C., Del Casino, C., Emons, A.M., and Cresti, M. (2011). Distribution of callose synthase, cellulose synthase, and sucrose synthase in tobacco pollen tube is controlled in dissimilar ways by actin filaments and microtubules. Plant Physiol. 155: 1169-1190. Carlson, S.J., and Chourey, P.S. (1996). Evidence for plasma membrane-associated forms of sucrose synthase in maize. Mol Gen Genet. 252: 303-310. Chang, W.W., Huang, L., Shen, M., Webster, C., Burlingame, A.L., and Roberts, J.K. (2000). Patterns of protein synthesis and tolerance of anoxia in root tips of maize seedlings acclimated to a low-oxygen environment, and identification of proteins by mass spectrometry. Plant Physiol. 122: 295-318. Chourey, P.S., Taliercio, E.W., Carlson, S.J., and Ruan, Y.L. (1998). Genetic evidence that the two isozymes of sucrose synthase present in developing maize endosperm are critical, one for cell wall integrity and the other for starch biosynthesis. Mol Gen Genet. 259: 88-96. Chung, B.Y.W., Simons, C., Firth, A.E., Brown, C.M., and Hellens, R.P. (2006). Effect of 5'UTR introns on gene expression in Arabidopsis thaliana. Bmc Genomics 7: 120. Davies, D.D., Grego, S., and Kenworth.P. (1974). Control of Production of Lactate and Ethanol by Higher-Plants. Planta 118: 297-310. Dejardin, A., Sokolov, L.N., and Kleczkowski, L.A. (1999). Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis. Biochem J. 344: 503-509. Dejardin, A., Rochat, C., Maugenest, S., and Boutin, J.P. (1997). Purification, characterization and physiological role of sucrose synthase in the pea seed coat (Pisum sativum L.). Planta 201: 128-137. Dennis, E.S., Dolferus, R., Ellis, M., Rahman, M., Wu, Y., Hoeren, F.U., Grover, A., Ismond, K.P., Good, A.G., and Peacock, W.J. (2000). Molecular strategies for improving waterlogging tolerance in plants. Journal of Experimental Botany 51: 89-97. Drew, M.C. (1997). OXYGEN DEFICIENCY AND ROOT METABOLISM: Injury and Acclimation Under Hypoxia and Anoxia. Annu Rev Plant Physiol Plant Mol Biol. 48: 223-250. Etxeberria, E., and Gonzalez, P. (2003). Evidence for a tonoplast-associated form of sucrose synthase and its potential involvement in sucrose mobilization from the vacuole. J Exp Bot. 54: 1407-1414. Etxeberria, E., Gonzalez, P., and Pozueta-Romero, J. (2005). Sucrose transport into citrus juice cells: Evidence for an endocytic transport system. J Am Soc Hortic Sci. 130: 269-274. Feder, M.E., and Hofmann, G.E. (1999). Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology. Annu Rev Physiol. 61: 243-282. Fukao, T., and Bailey-Serres, J. (2008). Submergence tolerance conferred by Sub1A is mediated by SLR1 and SLRL1 restriction of gibberellin responses in rice. Proc Natl Acad Sci U S A 105: 16814-16819. Fukao, T., Xu, K., Ronald, P.C., and Bailey-Serres, J. (2006). A variable cluster of ethylene response factor-like genes regulates metabolic and developmental acclimation responses to submergence in rice. Plant Cell 18: 2021-2034. Geigenberger, P. (2003). Response of plant metabolism to too little oxygen. Curr Opin Plant Biol. 6: 247-256. Germain, V., Ricard, B., Raymond, P., and Saglio, P.H. (1997). The Role of Sugars, Hexokinase, and Sucrose Synthase in the Determination of Hypoxically Induced Tolerance to Anoxia in Tomato Roots. Plant Physiol. 114: 167-175. Gibbs, J., and Greenway, H. (2003). Mechanisms of anoxia tolerance in plants. I. Growth, survival and anaerobic catabolism. Funct Plant Biol. 30: 1-47. Gray, N.K., and Wickens, M. (1998). Control of translation initiation in animals. Annu Rev Cell Dev Biol. 14: 399-458. Hardin, S.C., Winter, H., and Huber, S.C. (2004). Phosphorylation of the amino terminus of maize sucrose synthase in relation to membrane association and enzyme activity. Plant Physiol. 134: 1427-1438. Hohnjec, N., Becker, J.D., Puhler, A., Perlick, A.M., and Kuster, H. (1999). Genomic organization and expression properties of the MtSucS1 gene, which encodes a nodule-enhanced sucrose synthase in the model legume Medicago truncatula. Mol Gen Genet. 261: 514-522. Huang, S., Colmer, T.D., and Millar, A.H. (2008). Does anoxia tolerance involve altering the energy currency towards PPi? Trends Plant Sci. 13: 221-227. Hughes, T.A. (2006). Regulation of gene expression by alternative untranslated regions. Trends Genet. 22: 119-122. Ismond, K.P., Dolferus, R., de Pauw, M., Dennis, E.S., and Good, A.G. (2003). Enhanced low oxygen survival in Arabidopsis through increased metabolic flux in the fermentative pathway. Plant Physiol. 132: 1292-1302. Kleines, M., Elster, R.C., Rodrigo, M.J., Blervacq, A.S., Salamini, F., and Bartels, D. (1999). Isolation and expression analysis of two stress-responsive sucrose-synthase genes from the resurrection plant Craterostigma plantagineum (Hochst.). Planta 209: 13-24. Klok, E.J., Wilson, I.W., Wilson, D., Chapman, S.C., Ewing, R.M., Somerville, S.C., Peacock, W.J., Dolferus, R., and Dennis, E.S. (2002). Expression profile analysis of the low-oxygen response in Arabidopsis root cultures. Plant Cell 14: 2481-2494. Liu, F., Vantoai, T., Moy, L.P., Bock, G., Linford, L.D., and Quackenbush, J. (2005). Global transcription profiling reveals comprehensive insights into hypoxic response in Arabidopsis. Plant Physiol. 137: 1115-1129. Marana, C., Garcia-Olmedo, F., and Carbonero, P. (1990). Differential expression of two types of sucrose synthase-encoding genes in wheat in response to anaerobiosis, cold shock and light. Gene 88: 167-172. Martin, T., Frommer, W.B., Salanoubat, M., and Willmitzer, L. (1993). Expression of an Arabidopsis sucrose synthase gene indicates a role in metabolization of sucrose both during phloem loading and in sink organs. Plant J. 4: 367-377. Mommer, L., Pons, T.L., Wolters-Arts, M., Venema, J.H., and Visser, E.J.W. (2005). Submergence-induced morphological, anatomical, and biochemical responses in a terrestrial species affect gas diffusion resistance and photosynthetic performance. Plant Physiology 139: 497-508. Mustroph, A., Lee, S.C., Oosumi, T., Zanetti, M.E., Yang, H., Ma, K., Yaghoubi-Masihi, A., Fukao, T., and Bailey-Serres, J. (2010). Cross-kingdom comparison of transcriptomic adjustments to low-oxygen stress highlights conserved and plant-specific responses. Plant Physiol. 152: 1484-1500. Nakazawa, M., and Matsui, M. (2003). Selection of hygromycin-resistant Arabidopsis seedlings. Biotechniques 34, 28-30. Nolte, K.D., and Koch, K.E. (1993). Companion-Cell Specific Localization of Sucrose Synthase in Zones of Phloem Loading and Unloading. Plant Physiol. 101: 899-905. Olive, M.R., Peacock, W.J., and Dennis, E.S. (1991). The anaerobic responsive element contains two GC-rich sequences essential for binding a nuclear protein and hypoxic activation of the maize Adh1 promoter. Nucleic Acids Res. 19: 7053-7060. Olive, M.R., Walker, J.C., Singh, K., Dennis, E.S., and Peacock, W.J. (1990). Functional properties of the anaerobic responsive element of the maize Adh1 gene. Plant Mol Biol. 15: 593-604. Ruan, Y.L., Llewellyn, D.J., and Furbank, R.T. (2003). Suppression of sucrose synthase gene expression represses cotton fiber cell initiation, elongation, and seed development. Plant Cell 15: 952-964. Sachs, M.M., Subbaiah, C.C., and Saab, I.N. (1996). Anaerobic gene expression and flooding tolerance in maize. Journal of Experimental Botany 47, 1-15. Salnikov, V.V., Grimson, M.J., Delmer, D.P., and Haigler, C.H. (2001). Sucrose synthase localizes to cellulose synthesis sites in tracheary elements. Phytochemistry 57: 823-833. Subbaiah, C.C., and Sachs, M.M. (2003). Molecular and cellular adaptations of maize to flooding stress. Ann Bot. 91: 119-127. Sun, J., Loboda, T., Sung, S.J., and Black, C.C. (1992). Sucrose Synthase in Wild Tomato, Lycopersicon chmielewskii, and Tomato Fruit Sink Strength. Plant Physiol. 98: 1163-1169. Voesenek, L.A.C.J., Rijnders, J.H.G.M., Peeters, A.J.M., Van de Steeg, H.M.V., and De Kroon, H. (2004). Plant hormones regulate fast shoot elongation under water: From genes to communities. Ecology 85: 16-27. Wilkie, G.S., Dickson, K.S., and Gray, N.K. (2003). Regulation of mRNA translation by 5'- and 3'-UTR-binding factors. Trends Biochem Sci. 28: 182-188. Winter, H., Huber, J.L., and Huber, S.C. (1997). Membrane association of sucrose synthase: changes during the graviresponse and possible control by protein phosphorylation. FEBS Lett. 420: 151-155. Xu, K., Xu, X., Fukao, T., Canlas, P., Maghirang-Rodriguez, R., Heuer, S., Ismail, A.M., Bailey-Serres, J., Ronald, P.C., and Mackill, D.J. (2006). Sub1A is an ethylene-response-factor-like gene that confers submergence tolerance to rice. Nature 442: 705-708. Zeng, Y., Wu, Y., Avigne, W.T., and Koch, K.E. (1998). Differential regulation of sugar-sensitive sucrose synthases by hypoxia and anoxia indicate complementary transcriptional and posttranscriptional responses. Plant Physiol 116: 1573-1583. Zeng, Y., Wu, Y., Avigne, W.T., and Koch, K.E. (1999). Rapid repression of maize invertases by low oxygen. Invertase/sucrose synthase balance, sugar signaling potential, and seedling survival. Plant Physiol. 121: 599-608. Zhang, Z., Zhang, D., and Zheng, Y. (2009). Transcriptional and post-transcriptional regulation of gene expression in submerged root cells of maize. Plant Signal Behav. 4: 132-135. Zrenner, R., Salanoubat, M., Willmitzer, L., and Sonnewald, U. (1995). Evidence of the crucial role of sucrose synthase for sink strength using transgenic potato plants (Solanum tuberosum L.). Plant J. 7: 97-107. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48043 | - |
dc.description.abstract | 因氧氣在水中的擴散速率約為在大氣中的萬分之一,故植物遭受水患時,即對細胞造成缺氧逆境。氧氣不足,使得細胞代謝作用由有氧呼吸轉換為發酵反應來產生能量,因此參予在醣類移動與發酵反應相關的基因均被活化而大量表現,其中Sucrose synthase (SUS)酵素負責催化蔗糖分解為果糖與尿嘧啶雙磷酸葡萄糖,提供基質予下游的糖解作用與發酵反應。雖前人研究對SUS的功能已進行詳細的分析,但針對缺氧時,SUS基因的活化機制仍尚未釐清。故先使用semi-quantitative PCR的方式檢測SUS基因在植物根部遭遇水患時的表現情況,結果指出根部組織的SUS1與SUS4基因表現確實在缺氧逆境下被活化,且作用於轉錄層次的調控。接著利用5’ deletion 的方式分析SUS.11與SUS4的啟動子,並以luciferase做為報導基因,企圖找出參予缺氧調控機制的cis-element ,進一步了解其細胞訊息傳導的路徑。實驗結果指出距離轉錄起始點-1000到-600的區域,可能具有cis-element參予缺氧時調控SUS4基因表現的機制; 而針對SUS1.1基因,則是-1000到-300區域。 | zh_TW |
dc.description.abstract | Oxygen diffusion is about 10 000 times slower in water than that in air. When plants were subjected to flooding the root cells switch from aerobic respiration to anaerobic fermentation in response to oxygen deprivation (hypoxia). Cellular regulation was performed to increase the transcript levels of genes that are involved in mobilization of sugars, glycolysis and fermentation pathway. During submergence the expression of sucrose synthase (SUS) gene was up-regulated to catalyze degradation of sucrose into fructose and UDP-glucose, which are substrates for glycolysis. Although a large number of studies have been made on sucrose synthase gene function, little is known about the mechanism of transcriptional regulation of SUS genes under flooding. Here, we characterize gene expression patterns of the SUS gene family in Arabidopsis during flooding. The transcript levels of SUS1 and SUS4 increased in roots but in different manners during flooding. Expression cassettes with truncated promoter fragments of SUS1 and SUS4 linked to firefly (Photinus pyralis) luciferase coding sequence were also developed to detect promoter activity. The results from the 5’ promoter deletion analyses suggested that the region between -1000 and -600 (from transcription start site) in promoter of SUS4 was essential for flooding-induced gene expression. For SUS1 gene, the critical cis-element(s) may locate on -600~-300 region for transcriptional regulation under flooding. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T06:45:01Z (GMT). No. of bitstreams: 1 ntu-100-R98b42012-1.pdf: 3864513 bytes, checksum: 16c1da1ae8467238feec7baa35360c0d (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 口試委員審定書
誌謝 中文摘要 i Abstract ii Contents iv Index of tables vii Index of figures viii Chapter 1: Background knowledge 1 1.1 Flood is a worldwide stress for plants 1 1.2 The strategies for plants to survive flooding 2 1.3 Cellular acclimation in response to hypoxia 4 1.4 Sucrose synthase is the pivot of sucrose catabolism 7 1.5 The sucrose synthase gene family in Arabidopsis 9 Chapter 2: Materials and methods 11 1.1 Plants material and growth condition 11 1.2 Flooding treatment 11 1.3 Total RNA preparation and reverse transcription 12 1.4 Semi-quantification polymerase chain reaction (PCR) 13 1.5 Construction of 1kbp promoter of genes fusion to b-glucurodinase (GUS) gene 13 1.6 Construction of chimeric genes and binary vectors for transformation 14 1.7 Agrobacterium tumefaciens-competent cell preparation and electroporation 14 1.8 Agrobacterium-mediated transformation 15 1.9 Histochemical GUS assay 16 1.10 Luciferase activity assay 17 1.11 Analysis of transformants and interpretation of results 18 Chapter 3: Results and discussions 19 3.1 Induction of SUS1 and SUS4 gene expression in roots during flooding 19 3.2 The transcript levels of two splice variants of SUS1 increased in roots under flooding 20 3.3 The spatial expression pattern of SUS1 and SUS4 by histochemical GUS assay 21 3.4 A 1000 bp fragment of SUS1.1 promoter conferred the flooding induced expression 22 3.5 5’ Promoter deletion assay of SUS1.1 23 3.6 The 1000 bp fragment of SUS1.2 promoter can’t confer the flooding induction of luciferus reporter gene 24 3.7 A 1000 bp fragment of SUS4 promoter conferred flooding inducd expression pattern 25 3.8 5’ Promoter deletion assay of SUS4 26 Chapter 4: Concluding Remarks and future respective 27 Chapter 5: Tables and figures 29 References 49 | |
dc.language.iso | en | |
dc.title | 探討淹水逆境於阿拉伯芥SUS1與SUS4基因之轉錄調控 | zh_TW |
dc.title | Investigation of Transcriptional Regulation of AtSUS1 and AtSUS4 in Arabidopsis during Flooding | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 葉開溫(Kai-Wun Yeh),王淑美(Shue-Mei Wang) | |
dc.subject.keyword | 水患,阿拉伯芥,轉錄調控,sucrose synthase,5’promoter deletion assay, | zh_TW |
dc.subject.keyword | hypoxia,Arabidopsis,transcriptional regulation,sucrose synthase,5’promoter deletion assay, | en |
dc.relation.page | 53 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-06-28 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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