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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳家揚 | |
dc.contributor.author | Chiung-Wen Chang | en |
dc.contributor.author | 張瓊文 | zh_TW |
dc.date.accessioned | 2021-06-15T06:43:15Z | - |
dc.date.available | 2013-10-03 | |
dc.date.copyright | 2011-10-03 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-07-06 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47940 | - |
dc.description.abstract | 赭麴毒素A(Ochratoxin A, OTA)具腎毒性,廣泛存在於食物中,例如咖啡豆。因咖啡豆烘焙過程無法有效除去當中原有的OTA,故飲用咖啡飲品可能會暴露到OTA。
本研究開發以電灑游離(electrospray ionization, ESI)串聯式質譜儀搭配極致液相層析與同位素稀釋技術定量咖啡飲品中OTA之方法。經質譜儀參數適化並偵測OTA最佳母離子(precursor ion, [M+H]+)和兩個訊號最強子離子(product ion)。 前處理部分,添加經同位素標定之OTA內標準品至10 mL咖啡樣本中,離心後取上清液,以三倍體積磷酸緩衝液稀釋,續以每秒1滴之流速通過免疫親合性管柱(immunity affinity column, IAC),然後用去離子蒸餾水流洗管柱,再用甲醇沖提。沖提液經過濾並減壓離心濃縮至近乾,回溶後上機分析。層析管柱為Ascentis Express RP-amide,流速1.0 mL/min,移動相為0.1% 甲酸水溶液與乙腈,層析溫度為40℃,使用梯度流析,連同管柱再平衡之層析時間為3分鐘。 本研究樣本回收率範圍為84.5–117%,相對標準差(RSD)小於15%。基質效應結果顯示樣本訊號強度受到43%的抑制,而同位素稀釋技術可有效準確定量並消除基質效應對於定量之影響。咖啡樣本於同日異日重複分析之RSD皆低於15%。偵測極限和定量極限分別為0.52 pg/mL及1.73 pg/mL。 為瞭解市售咖啡飲品中OTA含量情形,本研究於2010年三月底到五月份中旬至便利商店和連鎖咖啡店購入現煮咖啡40件、罐裝即飲咖啡24件、即溶咖啡6件,共70件樣本,其中檢出66件,濃度範圍為2.0–131 pg/mL;現煮咖啡平均濃度較低,即溶咖啡平均濃度較高。一般而言,建議攝取咖啡飲品時儘量選擇由新鮮咖啡豆沖泡之現煮咖啡;若飲用罐裝即飲咖啡或即溶咖啡,建議一天以不超過4–5杯為宜。 | zh_TW |
dc.description.abstract | Ochratoxin A (OTA) is common contaminant in food resulting from a secondary metabolite of fungi, and the International Agency for Research on Cancer (IARC) has classified OTA as a possible human carcinogen (2B). The processes of baking coffee beans are not able to remove OTA completely. Humans are exposed to OTA via drinking coffee drinks.
This study developed and validated a method to determine the concentrations of OTA in coffee drinks using immunoaffinity column extraction and detection of ultra-high performance liquid chromatography/tandem mass spectrometry (UHPLC-MS/MS) with isotope-dilution techniques by 13C20-OTA as the internal standard. The OTA was analyzed on an Ascentis Express RP-amide column (50 | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T06:43:15Z (GMT). No. of bitstreams: 1 ntu-100-R98844006-1.pdf: 783014 bytes, checksum: b2e223ae7ad5ed6429a671599b872491 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 誌謝 I
摘要 II Abstract III 目錄 V 圖目錄 VII 表目錄 VIII 第一章 前言 1 第一節 研究源起 1 第二節 研究目的 3 第二章 文獻回顧 4 第一節 赭麴毒素A簡介 4 第二節 赭麴毒素A的汙染範圍與暴露途徑 5 第三節 赭麴毒素A之毒性與法規限值 6 第四節 黴菌毒素赭麴毒素A的檢測分析方法 8 第五節 液相層析串聯式質譜儀作用機制 10 第三章 材料與方法及實驗設計 14 第一節 試劑與材料 14 第二節 儀器設備 15 第三節 樣本前處理與儀器分析 16 第四節 分析方法確效 18 3-4-1分析樣本的回收率 18 3-4-2基質效應評估 18 3-4-3 咖啡樣本同日異日分析的準確性與精密度 19 3-4-4 方法偵測極限與定量極限 19 第五節 品保與品管(QA/QC) 20 第六節 資料處理 20 第四章 結果與討論 21 第一節 儀器分析條件最適化 21 第二節 樣本前處理和方法確效 24 第三節 咖啡樣本分析濃度 25 第四節 估算國人透過飲用咖啡可能攝食OTA毒素含量 26 第五章 結論與建議 29 參考文獻 31 圖 42 表 50 | |
dc.language.iso | zh-TW | |
dc.title | 以免疫親和管柱與極致液相層析/串聯式質譜儀搭配同位素稀釋技術分析市售咖啡飲品中赭麴毒素A | zh_TW |
dc.title | Determination of Ochratoxin A in Coffee Drinks using Immunoaffinity Cleanup and Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with Isotope-Dilution Techniques | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 呂廷璋,吳焜裕 | |
dc.subject.keyword | 免疫親合性管柱,液相層析/質譜/質譜儀,同位素稀釋技術,電灑游離, | zh_TW |
dc.subject.keyword | Ochratoxin A,immunoaffinity column,UHPLC-MS/MS,coffee, | en |
dc.relation.page | 55 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-07-06 | |
dc.contributor.author-college | 公共衛生學院 | zh_TW |
dc.contributor.author-dept | 環境衛生研究所 | zh_TW |
顯示於系所單位: | 環境衛生研究所 |
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