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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 林讚標 | |
| dc.contributor.author | Yin-Chu Chen | en |
| dc.contributor.author | 陳映竹 | zh_TW |
| dc.date.accessioned | 2021-06-15T06:42:56Z | - |
| dc.date.available | 2013-07-25 | |
| dc.date.copyright | 2011-07-25 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-07-07 | |
| dc.identifier.citation | Apel, K., and Hirt, H. (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology 55: 373-399.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47917 | - |
| dc.description.abstract | 麩胺基硫轉移酶 (glutathione S-transferases; GST) 被熟知參與植物面對氧化逆境。然而，對於GST基因在植物體中所扮演的調控功能，目前所知仍然有限。近來我們實驗室團隊發現當GST基因tau家族中的AtGSTU17基因缺失時，植株對於鹽及乾旱的耐受性相較於野生型更為提升。有此外表型的產生主要因為AtGSTU17基因缺失時，植物體中glutathione和abscisic acid增加，對外表型產生影響。因此我們推測AtGSTU17 於植物體面對非生物性逆境時，扮演負調節者的角色。我們希望了解atgstu17所產生的外表型，是否也會出現於tau家族其他基因缺失時。我們使用atgstu7, atgstu8, atgstu18進行研究，觀察其在非生物性逆境下之功能。經由一系列非生物性逆境測試後，發現此三個基因缺失之植株於乾旱及鹽處理下，表現出和atgstu17 不同的外表型。然而在abscisic acid處理、滲透壓逆境及鹽逆境下，相對於野生型植株則有較高的發芽率。推論AtGSTU7、AtGSTU8、AtGSTU18可能於逆境之下浸潤後的種子以及幼苗發育初期，扮演負調控者的角色。此外篩選atgstu25 homozygote植株的過程中，發現取用atgstu25 hemizygote產生的子代進行分析，得到WT : hemizygote : homozygote = 1:2:0顯示當AtGSTU25缺失時，可能造成致死現象。 | zh_TW |
| dc.description.abstract | Glutathione S-transferases (GSTs) have been well-documented to be involved in oxidative stress metabolism. However, reports on GST gene participating in regulatory function are limited. Previously our lab had found that glutathione S-transferase U17 (AtGSTU17) knock-out plants were more tolerant to drought and salt stresses than wild-type plants. The mechanism causing these phenotypes of atgstu17 can be explained mostly by the combined effect of elevated contents of both glutathione and abscisic acid. Thus, AtGSTU17 plays a negative role in regulating abiotic stress tolerance. Three homozygous knockout mutants, atgstu7, atgstu8, atgstu18, were chosen for further study with their function in response to abiotic stress. Preliminary stress experiments indicated that the mentioned above atgstu mutants showed different phenotypes from the atgstu17 mutant plants in terms of salt and drought tolerance. However, the germination rates of the three mutants were higher than wild-type plants when they were treated with abscisic acid, salt, and osmotic stress. We suggested that these three genes might play a negative regulatory role during seed imbibitions and seedling development under stress conditions. Genetic analysis of F2 segergating population indicated that wild-type (AtGSTU25/AtGSTU25), hemizygous (AtGSTU25/atgstu25), and homozygous mutant (atgstu25/atgstu25) plants were in a ratio of 1:2:0, indicating that the homozygous mutant allele is lethal. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T06:42:56Z (GMT). No. of bitstreams: 1 ntu-100-R98b42003-1.pdf: 9827487 bytes, checksum: 6bda84c7e65e20a9a7bf77cf92dd640c (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 誌謝 III
中文摘要 IV Abstract V 縮寫表 VI 第一章 序論 1 前言 1 1.1 植物於逆境下之氧化還原反應及常見抗氧化物 1 1.2 GSH與GST的關係 2 1.3 GSH於植物遭受逆境時所扮演的角色 2 1.4 植物GSTs 的歷史及分類 3 1.5 植物GSTs 的功能 4 1.6 GST tau家族與非生物逆境的關係 5 1.7 研究目標 5 第二章 材料與方法 7 2.1 植物材料、生長條件 7 2.2 AtGSTU7、AtGSTU8、 AtGSTU18、 AtGSTU25 基因序列分析 7 2.3 DNA 萃取及Polymerase Chain Reaction (PCR) 擴大基因片段 7 2.4 分析T-DNA homozygous lines 9 2.5 RNA 萃取，RNase-free DNase I 處理及 cDNA 合成，和 RT-PCR 9 2.6 氧化逆境下的生長情況測試 11 2.7 非生物性逆境下之發芽測試 11 2.8 ABA處理下根部延長試驗 11 2.9 突變株的非生物性逆境耐受性分析 11 2.10 Glutathione萃取 12 2.11 去除果莢葉綠素 12 第三章 結果 14 3.1 atgstu7、atgstu8、atgstu18、atgstu25植株之取得 14 3.2 阿拉伯芥裡的AtGSTU7、AtGSTU8、AtGSTU18及AtGSTU25基因 14 3.3 atgstu7、atgstu8、atgstu18在甲基紫精(MV)處理下之生長情形 14 3.4 atgstu7、atgstu8、atgstu18於鹽逆境下之種子發芽率 15 3.5 atgstu7、atgstu8、atgstu18在滲透壓逆境下之發芽率測試 15 3.6 atgstu7、atgstu8、atgstu18在ABA處理下之種子發芽率 16 3.7 atgstu7、atgstu8、atgstu18在ABA處理下之根長度 16 3.8 atgstu7、atgstu8、atgstu18在乾旱逆境下之耐受性測試 17 3.9 atgstu7、atgstu8、atgstu18在高鹽下之耐受性測試 17 3.10 atgstu7、atgstu8、atgstu18與野生型之GSH及GSH/GSSG 18 3.11 篩選AtGSTU25 T-DNA 插入品系 18 3.12 T-DNA插入AtGSTU25異型合子果莢結實狀況 18 第四章 討論 19 4.1 AtGSTU7、 AtGSTU8、 AtGSTU18及AtGSTU25於阿拉伯芥表現的位置 19 4.2 atgstu7、 atgstu8、atgstu18在甲基紫精 (MV) 處理下之生長情形和野生型間差異不明顯 19 4.3 atgstu7、 atgstu8、atgstu18之發芽率 19 4.4 atgstu7、atgstu8、atgstu18與野生型於ABA處理下之根長未出現明顯差異 20 4.5 atgstu7、atgstu8、atgstu18中GSH含量及在乾旱、鹽逆境下之耐受性 20 4.6 atgstu25 基因型分析的結果及果莢結實狀況 21 第五章 參考文獻 23 第六章 圖表 30 附錄 44 | |
| dc.language.iso | zh-TW | |
| dc.subject | 耐鹽 | zh_TW |
| dc.subject | 麩胺基硫轉移酶 | zh_TW |
| dc.subject | 穀胱甘肽 | zh_TW |
| dc.subject | 氧化逆境 | zh_TW |
| dc.subject | 耐乾旱 | zh_TW |
| dc.subject | salt tolerance | en |
| dc.subject | Glutathione S-transferases | en |
| dc.subject | glutathione | en |
| dc.subject | oxidative stress | en |
| dc.subject | drought tolerance | en |
| dc.title | 分析阿拉伯芥AtGSTU7、AtGSTU8、AtGSTU18及AtGSTU25
等基因失去功能時在非生物逆境下之反應 | zh_TW |
| dc.title | Characterization of loss-of-function mutants of AtGSTU7, AtGSTU8, AtGSTU18 and AtGSTU25 of Arabidopsis thaliana in response to abiotic stresses | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鄭石通,靳宗洛,陳仁治,鄭萬興 | |
| dc.subject.keyword | 麩胺基硫轉移酶,穀胱甘肽,氧化逆境,耐乾旱,耐鹽, | zh_TW |
| dc.subject.keyword | Glutathione S-transferases,glutathione,oxidative stress,drought tolerance,salt tolerance, | en |
| dc.relation.page | 57 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-07-07 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| Appears in Collections: | 植物科學研究所 | |
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