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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 黃銓珍 | |
dc.contributor.author | Syue-Lin Lyu | en |
dc.contributor.author | 呂學霖 | zh_TW |
dc.date.accessioned | 2021-06-15T06:42:46Z | - |
dc.date.available | 2012-07-25 | |
dc.date.copyright | 2011-07-25 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-07-07 | |
dc.identifier.citation | Abdalla, S.A., Gallione, C.J., Barst, R.J., Horn, E.M., Knowles, J.A., Marchuk, D.A., Letarte, M., and Morse, J.H. (2004). Primary pulmonary hypertension in families with hereditary haemorrhagic telangiectasia. Eur Respir J 23, 373-377.
Abdalla, S.A., and Letarte, M. (2006). Hereditary haemorrhagic telangiectasia: current views on genetics and mechanisms of disease. J Med Genet 43, 97-110. Adams, J., and Lawler, J. (1993). Extracellular matrix: the thrombospondin family. Curr Biol 3, 188-190. Adams, J.C., and Lawler, J. (2004). The thrombospondins. Int J Biochem Cell Biol 36, 961-968. Adams, J.C., Monk, R., Taylor, A.L., Ozbek, S., Fascetti, N., Baumgartner, S., and Engel, J. (2003). Characterisation of Drosophila thrombospondin defines an early origin of pentameric thrombospondins. J Mol Biol 328, 479-494. Adams, R.H., and Alitalo, K. (2007). Molecular regulation of angiogenesis and lymphangiogenesis. Nat Rev Mol Cell Biol 8, 464-478. Alitalo, K., and Carmeliet, P. (2002). Molecular mechanisms of lymphangiogenesis in health and disease. Cancer Cell 1, 219-227. Alitalo, K., Tammela, T., and Petrova, T.V. (2005). Lymphangiogenesis in development and human disease. Nature 438, 946-953. Armulik, A., Abramsson, A., and Betsholtz, C. (2005). Endothelial/pericyte interactions. Circ Res 97, 512-523. Bautch, V.L. (2009). Endothelial cells form a phalanx to block tumor metastasis. Cell 136, 810-812. Benjamin, L.E., Golijanin, D., Itin, A., Pode, D., and Keshet, E. (1999). Selective ablation of immature blood vessels in established human tumors follows vascular endothelial growth factor withdrawal. J Clin Invest 103, 159-165. Bentley, K., Mariggi, G., Gerhardt, H., and Bates, P.A. (2009). Tipping the balance: robustness of tip cell selection, migration and fusion in angiogenesis. PLoS Comput Biol 5, e1000549. Bornstein, P. (1995). Diversity of function is inherent in matricellular proteins: an appraisal of thrombospondin 1. J Cell Biol 130, 503-506. Bornstein, P. (2001). Thrombospondins as matricellular modulators of cell function. J Clin Invest 107, 929-934. Bornstein, P., Armstrong, L.C., Hankenson, K.D., Kyriakides, T.R., and Yang, Z. (2000). Thrombospondin 2, a matricellular protein with diverse functions. Matrix Biol 19, 557-568. Bruneel, A., Labas, V., Mailloux, A., Sharma, S., Vinh, J., Vaubourdolle, M., and Baudin, B. (2003). Proteomic study of human umbilical vein endothelial cells in culture. Proteomics 3, 714-723. Carlson, C.B., Lawler, J., and Mosher, D.F. (2008). Structures of thrombospondins. Cell Mol Life Sci 65, 672-686. Carmeliet, P. (2005). Angiogenesis in life, disease and medicine. Nature 438, 932-936. Carmeliet, P., and Tessier-Lavigne, M. (2005). Common mechanisms of nerve and blood vessel wiring. Nature 436, 193-200. Chatila, K., Ren, G., Xia, Y., Huebener, P., Bujak, M., and Frangogiannis, N.G. (2007). The role of the thrombospondins in healing myocardial infarcts. Cardiovasc Hematol Agents Med Chem 5, 21-27. Clark, E.R. (1918). Studies on the growth of blood vessels in the tail of the frog larvae. Am J Anat 23, 37–88. Cleaver, O., Tonissen, K.F., Saha, M.S., and Krieg, P.A. (1997). Neovascularization of the Xenopus embryo. Dev Dyn 210, 66-77. Coffin, J.D., and Poole, T.J. (1988). Embryonic vascular development: immunohistochemical identification of the origin and subsequent morphogenesis of the major vessel primordia in quail embryos. Development 102, 735-748. Corless, C.L., Mendoza, A., Collins, T., and Lawler, J. (1992). Colocalization of thrombospondin and syndecan during murine development. Dev Dyn 193, 346-358. Culotti, J.G., Spence, A., Zhou, Y., Scott, L., Leung-Hagesteijn, B., and Hedgecook, E. (1991). The unc-5 axon guidance gene of C. elegans has features of a cell adhesion receptor. J Cell Biol 115, 122. Davis, S., Aldrich, T.H., Jones, P.F., Acheson, A., Compton, D.L., Jain, V., Ryan, T.E., Bruno, J., Radziejewski, C., Maisonpierre, P.C., et al. (1996). Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning. Cell 87, 1161-1169. de Vries, C., Escobedo, J.A., Ueno, H., Houck, K., Ferrara, N., and Williams, L.T. (1992). The fms-like tyrosine kinase, a receptor for vascular endothelial growth factor. Science 255, 989-991. Dixit, V.M., Hennessy, S.W., Grant, G.A., Rotwein, P., and Frazier, W.A. (1986). Characterization of a cDNA encoding the heparin and collagen binding domains of human thrombospondin. Proc Natl Acad Sci U S A 83, 5449-5453. Doetschman, T.C., Eistetter, H., Katz, M., Schmidt, W., and Kemler, R. (1985). The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J Embryol Exp Morphol 87, 27-45. Dumont, D.J., Gradwohl, G., Fong, G.H., Puri, M.C., Gertsenstein, M., Auerbach, A., and Breitman, M.L. (1994). Dominant-negative and targeted null mutations in the endothelial receptor tyrosine kinase, tek, reveal a critical role in vasculogenesis of the embryo. Genes Dev 8, 1897-1909. Engel, J. (1989). EGF-like domains in extracellular matrix proteins: localized signals for growth and differentiation? FEBS Lett 251, 1-7. Esemuede, N., Lee, T., Pierre-Paul, D., Sumpio, B.E., and Gahtan, V. (2004). The role of thrombospondin-1 in human disease. J Surg Res 122, 135-142. Fan, L., and Collodi, P. (2006). Zebrafish embryonic stem cells. Methods Enzymol 418, 64-77. Fernandez, L.A., Sanz-Rodriguez, F., Blanco, F.J., Bernabeu, C., and Botella, L.M. (2006). Hereditary hemorrhagic telangiectasia, a vascular dysplasia affecting the TGF-beta signaling pathway. Clin Med Res 4, 66-78. Ferrara, N. (1999). Molecular and biological properties of vascular endothelial growth factor. J Mol Med 77, 527-543. Ferrara, N., Gerber, H.P., and LeCouter, J. (2003). The biology of VEGF and its receptors. Nat Med 9, 669-676. Ferrell, R.E. (2002). Research perspectives in inherited lymphatic disease. Ann N Y Acad Sci 979, 39-51; discussion 76-39. Fong, G.H., Rossant, J., Gertsenstein, M., and Breitman, M.L. (1995). Role of the Flt-1 receptor tyrosine kinase in regulating the assembly of vascular endothelium. Nature 376, 66-70. Fong, G.H., Zhang, L., Bryce, D.M., and Peng, J. (1999). Increased hemangioblast commitment, not vascular disorganization, is the primary defect in flt-1 knock-out mice. Development 126, 3015-3025. Friedl, P., Vischer, P., and Freyberg, M.A. (2002). The role of thrombospondin-1 in apoptosis. Cell Mol Life Sci 59, 1347-1357. Frolova, E.G., Pluskota, E., Krukovets, I., Burke, T., Drumm, C., Smith, J.D., Blech, L., Febbraio, M., Bornstein, P., Plow, E.F., et al. (2010). Thrombospondin-4 regulates vascular inflammation and atherogenesis. Circ Res 107, 1313-1325. Gabrielsen, A., Lawler, P.R., Yongzhong, W., Steinbruchel, D., Blagoja, D., Paulsson-Berne, G., Kastrup, J., and Hansson, G.K. (2007). Gene expression signals involved in ischemic injury, extracellular matrix composition and fibrosis defined by global mRNA profiling of the human left ventricular myocardium. J Mol Cell Cardiol 42, 870-883. Gaengel, K., Genove, G., Armulik, A., and Betsholtz, C. (2009). Endothelial-mural cell signaling in vascular development and angiogenesis. Arterioscler Thromb Vasc Biol 29, 630-638. Gallione, C.J., Repetto, G.M., Legius, E., Rustgi, A.K., Schelley, S.L., Tejpar, S., Mitchell, G., Drouin, E., Westermann, C.J., and Marchuk, D.A. (2004). A combined syndrome of juvenile polyposis and hereditary haemorrhagic telangiectasia associated with mutations in MADH4 (SMAD4). Lancet 363, 852-859. Gerhardt, H., and Betsholtz, C. (2003). Endothelial-pericyte interactions in angiogenesis. Cell Tissue Res 314, 15-23. Gerhardt, H., Golding, M., Fruttiger, M., Ruhrberg, C., Lundkvist, A., Abramsson, A., Jeltsch, M., Mitchell, C., Alitalo, K., Shima, D., et al. (2003). VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia. J Cell Biol 161, 1163-1177. Gissel, C., Nierhoff, D., Fleischmann, B., Hescheler, J., and Sachinidis, A. (2005). Culture of Embryoid Bodies. Practical Methods in Cardiovascular Research 2, 577-591. Goldie, L.C., Nix, M.K., and Hirschi, K.K. (2008). Embryonic vasculogenesis and hematopoietic specification. Organogenesis 4, 257-263. Gong, Z., Ju, B., Wang, X., He, J., Wan, H., Sudha, P.M., and Yan, T. (2002). Green fluorescent protein expression in germ-line transmitted transgenic zebrafish under a stratified epithelial promoter from keratin8. Dev Dyn 223, 204-215. Goundis, D., and Reid, K.B. (1988). Properdin, the terminal complement components, thrombospondin and the circumsporozoite protein of malaria parasites contain similar sequence motifs. Nature 335, 82-85. Greco, S.A., Chia, J., Inglis, K.J., Cozzi, S.J., Ramsnes, I., Buttenshaw, R.L., Spring, K.J., Boyle, G.M., Worthley, D.L., Leggett, B.A., et al. (2010). Thrombospondin-4 is a putative tumour-suppressor gene in colorectal cancer that exhibits age-related methylation. BMC Cancer 10, 494. Hankenson, K.D., Hormuzdi, S.G., Meganck, J.A., and Bornstein, P. (2005). Mice with a disruption of the thrombospondin 3 gene differ in geometric and biomechanical properties of bone and have accelerated development of the femoral head. Mol Cell Biol 25, 5599-5606. Hedbom, E., Antonsson, P., Hjerpe, A., Aeschlimann, D., Paulsson, M., Rosa-Pimentel, E., Sommarin, Y., Wendel, M., Oldberg, A., and Heinegard, D. (1992). Cartilage matrix proteins. An acidic oligomeric protein (COMP) detected only in cartilage. J Biol Chem 267, 6132-6136. Hirakow, R., and Hiruma, T. (1981). Scanning electron microscopic study on the development of primitive blood vessels in chick embryos at the early somite-stage. Anat Embryol (Berl) 163, 299-306. Hirschi, K.K., Rohovsky, S.A., and D'Amore, P.A. (1998). PDGF, TGF-beta, and heterotypic cell-cell interactions mediate endothelial cell-induced recruitment of 10T1/2 cells and their differentiation to a smooth muscle fate. J Cell Biol 141, 805-814. Iruela-Arispe, M.L., Liska, D.J., Sage, E.H., and Bornstein, P. (1993). Differential expression of thrombospondin 1, 2, and 3 during murine development. Dev Dyn 197, 40-56. Jin, S.W., Beis, D., Mitchell, T., Chen, J.N., and Stainier, D.Y. (2005). Cellular and molecular analyses of vascular tube and lumen formation in zebrafish. Development 132, 5199-5209. Joutel, A., Corpechot, C., Ducros, A., Vahedi, K., Chabriat, H., Mouton, P., Alamowitch, S., Domenga, V., Cecillion, M., Marechal, E., et al. (1996). Notch3 mutations in CADASIL, a hereditary adult-onset condition causing stroke and dementia. Nature 383, 707-710. Klagsbrun, M., Takashima, S., and Mamluk, R. (2002). The role of neuropilin in vascular and tumor biology. Adv Exp Med Biol 515, 33-48. Kondo, Y., Shen, L., Yan, P.S., Huang, T.H., and Issa, J.P. (2004). Chromatin immunoprecipitation microarrays for identification of genes silenced by histone H3 lysine 9 methylation. Proc Natl Acad Sci U S A 101, 7398-7403. Korkola, J.E., DeVries, S., Fridlyand, J., Hwang, E.S., Estep, A.L., Chen, Y.Y., Chew, K.L., Dairkee, S.H., Jensen, R.M., and Waldman, F.M. (2003). Differentiation of lobular versus ductal breast carcinomas by expression microarray analysis. Cancer Res 63, 7167-7175. Laherty, C.D., O'Rourke, K., Wolf, F.W., Katz, R., Seldin, M.F., and Dixit, V.M. (1992). Characterization of mouse thrombospondin 2 sequence and expression during cell growth and development. J Biol Chem 267, 3274-3281. Langley, R.R., and Fidler, I.J. (2007). Tumor cell-organ microenvironment interactions in the pathogenesis of cancer metastasis. Endocr Rev 28, 297-321. Latham, A.M., Molina-Paris, C., Homer-Vanniasinkam, S., and Ponnambalam, S. (2010). An integrative model for vascular endothelial growth factor A as a tumour biomarker. Integr Biol (Camb) 2, 397-407. Latker, C.H., Feinberg, R.N., and Beebe, D.C. (1986). Localized vascular regression during limb morphogenesis in the chicken embryo: II. Morphological changes in the vasculature. Anat Rec 214, 410-417, 392-413. Lawler, J., and Detmar, M. (2004). Tumor progression: the effects of thrombospondin-1 and -2. Int J Biochem Cell Biol 36, 1038-1045. Lawler, J., Duquette, M., Ferro, P., Copeland, N.G., Gilbert, D.J., and Jenkins, N.A. (1991). Characterization of the murine thrombospondin gene. Genomics 11, 587-600. Lawler, J., Duquette, M., Urry, L., McHenry, K., and Smith, T.F. (1993a). The evolution of the thrombospondin gene family. J Mol Evol 36, 509-516. Lawler, J., Duquette, M., Whittaker, C.A., Adams, J.C., McHenry, K., and DeSimone, D.W. (1993b). Identification and characterization of thrombospondin-4, a new member of the thrombospondin gene family. J Cell Biol 120, 1059-1067. Lawler, J., and Hynes, R.O. (1986). The structure of human thrombospondin, an adhesive glycoprotein with multiple calcium-binding sites and homologies with several different proteins. J Cell Biol 103, 1635-1648. Lawler, J., and Simons, E.R. (1983). Cooperative binding of calcium to thrombospondin. The effect of calcium on the circular dichroism and limited tryptic digestion of thrombospondin. J Biol Chem 258, 12098-12101. Lawler, J., Sunday, M., Thibert, V., Duquette, M., George, E.L., Rayburn, H., and Hynes, R.O. (1998). Thrombospondin-1 is required for normal murine pulmonary homeostasis and its absence causes pneumonia. J Clin Invest 101, 982-992. Lawson, N.D., Scheer, N., Pham, V.N., Kim, C.H., Chitnis, A.B., Campos-Ortega, J.A., and Weinstein, B.M. (2001). Notch signaling is required for arterial-venous differentiation during embryonic vascular development. Development 128, 3675-3683. Lawson, N.D., Vogel, A.M., and Weinstein, B.M. (2002). sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiation. Dev Cell 3, 127-136. Lawson, N.D., and Weinstein, B.M. (2002). In vivo imaging of embryonic vascular development using transgenic zebrafish. Dev Biol 248, 307-318. Levy, S., Todd, S.C., and Maecker, H.T. (1998). CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system. Annu Rev Immunol 16, 89-109. Lindahl, P., Johansson, B.R., Leveen, P., and Betsholtz, C. (1997). Pericyte loss and microaneurysm formation in PDGF-B-deficient mice. Science 277, 242-245. Lindsay, E.A., Vitelli, F., Su, H., Morishima, M., Huynh, T., Pramparo, T., Jurecic, V., Ogunrinu, G., Sutherland, H.F., Scambler, P.J., et al. (2001). Tbx1 haploinsufficieny in the DiGeorge syndrome region causes aortic arch defects in mice. Nature 410, 97-101. Lucitt, M.B., Price, T.S., Pizarro, A., Wu, W., Yocum, A.K., Seiler, C., Pack, M.A., Blair, I.A., Fitzgerald, G.A., and Grosser, T. (2008). Analysis of the zebrafish proteome during embryonic development. Mol Cell Proteomics 7, 981-994. Lv, B., Wang, H., Tang, Y., Fan, Z., Xiao, X., and Chen, F. (2009). High-mobility group box 1 protein induces tissue factor expression in vascular endothelial cells via activation of NF-kappaB and Egr-1. Thromb Haemost 102, 352-359. Ma, C., Fan, L., Ganassin, R., Bols, N., and Collodi, P. (2001). Production of zebrafish germ-line chimeras from embryo cell cultures. Proc Natl Acad Sci U S A 98, 2461-2466. Makino, S., Whitehead, G.G., Lien, C.L., Kim, S., Jhawar, P., Kono, A., Kawata, Y., and Keating, M.T. (2005). Heat-shock protein 60 is required for blastema formation and maintenance during regeneration. Proc Natl Acad Sci U S A 102, 14599-14604. Martinez-Pinna, R., Ramos-Mozo, P., Madrigal-Matute, J., Blanco-Colio, L.M., Lopez, J.A., Calvo, E., Camafeita, E., Lindholt, J.S., Meilhac, O., Delbosc, S., et al. (2011). Identification of peroxiredoxin-1 as a novel biomarker of abdominal aortic aneurysm. Arterioscler Thromb Vasc Biol 31, 935-943. Matsui, J., Wakabayashi, T., Asada, M., Yoshimatsu, K., and Okada, M. (2004). Stem cell factor/c-kit signaling promotes the survival, migration, and capillary tube formation of human umbilical vein endothelial cells. J Biol Chem 279, 18600-18607. Matsumoto, T., and Claesson-Welsh, L. (2001). VEGF receptor signal transduction. Sci STKE 2001, re21. Mazzone, M., Dettori, D., Leite de Oliveira, R., Loges, S., Schmidt, T., Jonckx, B., Tian, Y.M., Lanahan, A.A., Pollard, P., Ruiz de Almodovar, C., et al. (2009). Heterozygous deficiency of PHD2 restores tumor oxygenation and inhibits metastasis via endothelial normalization. Cell 136, 839-851. McKenzie, P., Chadalavada, S.C., Bohrer, J., and Adams, J.C. (2006). Phylogenomic analysis of vertebrate thrombospondins reveals fish-specific paralogues, ancestral gene relationships and a tetrapod innovation. BMC Evol Biol 6, 33. Meier, S. (1980). Development of the chick embryo mesoblast: pronephros, lateral plate, and early vasculature. J Embryol Exp Morphol 55, 291-306. Mustonen, E., Aro, J., Puhakka, J., Ilves, M., Soini, Y., Leskinen, H., Ruskoaho, H., and Rysa, J. (2008). Thrombospondin-4 expression is rapidly upregulated by cardiac overload. Biochem Biophys Res Commun 373, 186-191. Newton, G., Weremowicz, S., Morton, C.C., Copeland, N.G., Gilbert, D.J., Jenkins, N.A., and Lawler, J. (1994). Characterization of human and mouse cartilage oligomeric matrix protein. Genomics 24, 435-439. Nilsen, N.O. (1981). Microangiography in explanted chick embryos. Microvasc Res 22, 156-170. Nomura, N., Takahashi, M., Matsui, M., Ishii, S., Date, T., Sasamoto, S., and Ishizaki, R. (1988). Isolation of human cDNA clones of myb-related genes, A-myb and B-myb. Nucleic Acids Res 16, 11075-11089. O'Shea, K.S., and Dixit, V.M. (1988). Unique distribution of the extracellular matrix component thrombospondin in the developing mouse embryo. J Cell Biol 107, 2737-2748. Oldberg, A., Antonsson, P., Lindblom, K., and Heinegard, D. (1992). COMP (cartilage oligomeric matrix protein) is structurally related to the thrombospondins. J Biol Chem 267, 22346-22350. Oren, R., Takahashi, S., Doss, C., Levy, R., and Levy, S. (1990). TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins. Mol Cell Biol 10, 4007-4015. Paranko, J., Seitz, J., and Meinhardt, A. (1996). Developmental expression of heat shock protein 60 (HSP60) in the rat testis and ovary. Differentiation 60, 159-167. Pardanaud, L., Altmann, C., Kitos, P., Dieterlen-Lievre, F., and Buck, C.A. (1987). Vasculogenesis in the early quail blastodisc as studied with a monoclonal antibody recognizing endothelial cells. Development 100, 339-349. Phng, L.K., and Gerhardt, H. (2009). Angiogenesis: a team effort coordinated by notch. Dev Cell 16, 196-208. Pluskota, E., Stenina, O.I., Krukovets, I., Szpak, D., Topol, E.J., and Plow, E.F. (2005). Mechanism and effect of thrombospondin-4 polymorphisms on neutrophil function. Blood 106, 3970-3978. Poole, T.J., and Coffin, J.D. (1989). Vasculogenesis and angiogenesis: two distinct morphogenetic mechanisms establish embryonic vascular pattern. J Exp Zool 251, 224-231. Pusztaszeri, M.P., Seelentag, W., and Bosman, F.T. (2006). Immunohistochemical expression of endothelial markers CD31, CD34, von Willebrand factor, and Fli-1 in normal human tissues. J Histochem Cytochem 54, 385-395. Qabar, A.N., Lin, Z., Wolf, F.W., O'Shea, K.S., Lawler, J., and Dixit, V.M. (1994). Thrombospondin 3 is a developmentally regulated heparin binding protein. J Biol Chem 269, 1262-1269. Ramalho-Santos, M., Yoon, S., Matsuzaki, Y., Mulligan, R.C., and Melton, D.A. (2002). 'Stemness': transcriptional profiling of embryonic and adult stem cells. Science 298, 597-600. Riessen, R., Fenchel, M., Chen, H., Axel, D.I., Karsch, K.R., and Lawler, J. (2001). Cartilage oligomeric matrix protein (thrombospondin-5) is expressed by human vascular smooth muscle cells. Arterioscler Thromb Vasc Biol 21, 47-54. Risau, W. (1997). Mechanisms of angiogenesis. Nature 386, 671-674. Robles, V., Marti, M., and Izpisua Belmonte, J.C. (2011). Study of pluripotency markers in zebrafish embryos and transient embryonic stem cell cultures. Zebrafish 8, 57-63. Robson, K.J., Hall, J.R., Jennings, M.W., Harris, T.J., Marsh, K., Newbold, C.I., Tate, V.E., and Weatherall, D.J. (1988). A highly conserved amino-acid sequence in thrombospondin, properdin and in proteins from sporozoites and blood stages of a human malaria parasite. Nature 335, 79-82. Ruchoux, M.M., Domenga, V., Brulin, P., Maciazek, J., Limol, S., Tournier-Lasserve, E., and Joutel, A. (2003). Transgenic mice expressing mutant Notch3 develop vascular alterations characteristic of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy. Am J Pathol 162, 329-342. Ruhrberg, C., Gerhardt, H., Golding, M., Watson, R., Ioannidou, S., Fujisawa, H., Betsholtz, C., and Shima, D.T. (2002). Spatially restricted patterning cues provided by heparin-binding VEGF-A control blood vessel branching morphogenesis. Genes Dev 16, 2684-2698. Rysa, J., Leskinen, H., Ilves, M., and Ruskoaho, H. (2005). Distinct upregulation of extracellular matrix genes in transition from hypertrophy to hypertensive heart failure. Hypertension 45, 927-933. Sabin, F.C. (1920). Studies on the origin of blood-vessels and of red blood corpuscles as seen in the living blastoderm of chicks on the second day of incubation. Contrib Embryol 36, 213-261. Sato, T.N., Tozawa, Y., Deutsch, U., Wolburg-Buchholz, K., Fujiwara, Y., Gendron-Maguire, M., Gridley, T., Wolburg, H., Risau, W., and Qin, Y. (1995). Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation. Nature 376, 70-74. Schleicher, M., Shepherd, B.R., Suarez, Y., Fernandez-Hernando, C., Yu, J., Pan, Y., Acevedo, L.M., Shadel, G.S., and Sessa, W.C. (2008). Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence. J Cell Biol 180, 101-112. Shalaby, F., Rossant, J., Yamaguchi, T.P., Gertsenstein, M., Wu, X.F., Breitman, M.L., and Schuh, A.C. (1995). Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice. Nature 376, 62-66. Shepard, J.L., Amatruda, J.F., Stern, H.M., Subramanian, A., Finkelstein, D., Ziai, J., Finley, K.R., Pfaff, K.L., Hersey, C., Zhou, Y., et al. (2005). A zebrafish bmyb mutation causes genome instability and increased cancer susceptibility. Proc Natl Acad Sci U S A 102, 13194-13199. Si, Z., Palkama, A., Gebhardt, B.M., Velasquez, D., Galeano, M.J., and Beuerman, R.W. (2003). Distribution of thrombospondin-4 in the bovine eye. Curr Eye Res 27, 165-173. Stalmans, I., Lambrechts, D., De Smet, F., Jansen, S., Wang, J., Maity, S., Kneer, P., von der Ohe, M., Swillen, A., Maes, C., et al. (2003). VEGF: a modifier of the del22q11 (DiGeorge) syndrome? Nat Med 9, 173-182. Stenina, O.I., Desai, S.Y., Krukovets, I., Kight, K., Janigro, D., Topol, E.J., and Plow, E.F. (2003). Thrombospondin-4 and its variants: expression and differential effects on endothelial cells. Circulation 108, 1514-1519. Stratman, A.N., Malotte, K.M., Mahan, R.D., Davis, M.J., and Davis, G.E. (2009). Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation. Blood 114, 5091-5101. Suri, C., Jones, P.F., Patan, S., Bartunkova, S., Maisonpierre, P.C., Davis, S., Sato, T.N., and Yancopoulos, G.D. (1996). Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis. Cell 87, 1171-1180. Swift, M.R., and Weinstein, B.M. (2009). Arterial-venous specification during development. Circ Res 104, 576-588. Tan, F.L., Moravec, C.S., Li, J., Apperson-Hansen, C., McCarthy, P.M., Young, J.B., and Bond, M. (2002). The gene expression fingerprint of human heart failure. Proc Natl Acad Sci U S A 99, 11387-11392. Tanaka, Y., Patestos, N.P., Maekawa, T., and Ishii, S. (1999). B-myb is required for inner cell mass formation at an early stage of development. J Biol Chem 274, 28067-28070. Tarasov, K.V., Tarasova, Y.S., Tam, W.L., Riordon, D.R., Elliott, S.T., Kania, G., Li, J., Yamanaka, S., Crider, D.G., Testa, G., et al. (2008). B-MYB is essential for normal cell cycle progression and chromosomal stability of embryonic stem cells. PLoS One 3, e2478. Thompson, M.A., Ransom, D.G., Pratt, S.J., MacLennan, H., Kieran, M.W., Detrich, H.W., 3rd, Vail, B., Huber, T.L., Paw, B., Brownlie, A.J., et al. (1998). The cloche and spadetail genes differentially affect hematopoiesis and vasculogenesis. Dev Biol 197, 248-269. Tucker, R.P. (1993). The in situ localization of tenascin splice variants and thrombospondin 2 mRNA in the avian embryo. Development 117, 347-358. Tucker, R.P., Adams, J.C., and Lawler, J. (1995). Thrombospondin-4 is expressed by early osteogenic tissues in the chick embryo. Dev Dyn 203, 477-490. van Doorn, R., Zoutman, W.H., Dijkman, R., de Menezes, R.X., Commandeur, S., Mulder, A.A., van der Velden, P.A., Vermeer, M.H., Willemze, R., Yan, P.S., et al. (2005). Epigenetic profiling of cutaneous T-cell lymphoma: promoter hypermethylation of multiple tumor suppressor genes including BCL7a, PTPRG, and p73. J Clin Oncol 23, 3886-3896. Vogeli, K.M., Jin, S.W., Martin, G.R., and Stainier, D.Y. (2006). A common progenitor for haematopoietic and endothelial lineages in the zebrafish gastrula. Nature 443, 337-339. Vos, H.L., Devarayalu, S., de Vries, Y., and Bornstein, P. (1992). Thrombospondin 3 (Thbs3), a new member of the thrombospondin gene family. J Biol Chem 267, 12192-12196. Wang, H., Yang, H., Czura, C.J., Sama, A.E., and Tracey, K.J. (2001). HMGB1 as a late mediator of lethal systemic inflammation. Am J Respir Crit Care Med 164, 1768-1773. Witte, M.H., Bernas, M.J., Martin, C.P., and Witte, C.L. (2001). Lymphangiogenesis and lymphangiodysplasia: from molecular to clinical lymphology. Microsc Res Tech 55, 122-145. Yamaguchi, T.P., Dumont, D.J., Conlon, R.A., Breitman, M.L., and Rossant, J. (1993). flk-1, an flt-related receptor tyrosine kinase is an early marker for endothelial cell precursors. Development 118, 489-498. Zheng, P.P., Severijnen, L.A., van der Weiden, M., Willemsen, R., and Kros, J.M. (2009). A crucial role of caldesmon in vascular development in vivo. Cardiovasc Res 81, 362-369. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47902 | - |
dc.description.abstract | 血管內皮是一層緊密排列在各種血管內壁的表面細胞層,在血管空腔的循環血流與血管壁中形成一個交界面,內皮細胞在血管發育、凝血、免疫系統甚至在動脈硬化與癌症中都扮演著重要的角色。血管的正常發育明顯是胚胎發育的關鍵,然而在成人中血管發育的異常則容易導致疾病的產生,例如: 腫瘤的生長。另一方面,在人類疾病的診斷上生物標誌是一個被普遍運用的工具。Fli-1: EGFP基因轉殖斑馬魚在發育過程中,fli-1啟動子可以驅動EGFP表現在血管組織。在此研究中,我們希望能藉由Fli-1: EGFP基因轉殖斑馬魚胚胎來探索新的血管內皮細胞生物標誌。首先,我們個別收集並分離 4 hpf 與120 hpf 的Fli-1: EGFP基因轉殖斑馬魚胚胎,接著利用流式細胞分選儀 (FACSAria) 分選出帶有綠色螢光的血管內皮細胞。在進行分選前,先將受精後4小時Fli-1: EGFP的胚胎細胞與餵養細胞進行短暫共同培養來增加細胞數量。接著利用SDS-PAGE分離細胞萃取液後將蛋白質片段切下並進行膠內水解後再以 LC/MS/MS 分析,最後利用 MSCOT 資料庫(http://www.matrixscience.com ) 分析所得 MS 數據。血管內皮細胞在兩個不同發育階段中蛋白質型態呈現著不同的分布,比較實驗的數據,我們可以發現一些發育階段專一性蛋白的表現與可能的血管內皮細胞生物標誌,Thrombospondin-4A、4B。更進一步,我們製備針對斑馬魚Thrombospondin-4A、4B的抗體並透過免疫染色分析發現Thrombospondin-4A與已知的血管內皮細胞生物標誌 (VEGFR1、VEGFR2 與 Tie-2) 表達在相同的位置。Thrombospondin-4A具有可以在人類疾病管理上作為臨床診斷與預後生物標誌的潛能。 | zh_TW |
dc.description.abstract | The vascular endothelium is a thin layer of cells that lines the interior surface of blood vessels, forming an interface between circulating blood in the lumen and the rest of the vessel wall. Vascular endothelial cells play an important role in vascular development, blood coagulation, immune system and even in atherosclerosis and cancer. It has been reported that normal blood vessel formation is critical for embryonic development, whereas the aberrant blood vessel formation in the adult contributes to pathologies such as tumor growth. On the other hand, biomarker is a prevalent tool for early diagnosis of human diseases. Zebrafish fli-1 promoter is able to drive expression of EGFP in all blood vessels throughout embryogenesis. In this study, we intend to identify some novel biomarker of vascular endothelial cells by using Fli-1: EGFP transgenic zebrafish embryos. First, we collected and isolated cells of Fli-1: EGFP transgenic zebrafish embryo at 4hpf and 120hpf and then the EGFP positive vascular endothelial cells were sorted by Flow cytometry (FACSAria). The cells from 4hpf Fli-1 embryos were shortly co-cultured with feeder layer to increase the cell number (for expanding cell population) before sorting process. After resolving the total lysate from the sorted cells by SDS-PAGE, the separated proteins were subjected to trypsin in gel digestion followed by LC/MS/MS analysis. The data of proteomics analysis present the different protein patterns in these two stages. According to these data, we could identify some developmental stage-specific proteins and potential vascular endothelial cell biomarkers, Thrombospondin-4A and 4B. Furthermore, we clone the zebrafish TSP4A and TSP4B for the His-TSP4A and His-TSP4B fusion proteins and generate the antibodies against these antigens. By using immuno-staining, we could find out zebrafish Thrombospondin-4A respectively co-localize with VEGFR1、VEGFR2 and Tie-2. Thrombospondin-4A may be a biomarker for clinical diagnosis or prognosis in human disease management. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T06:42:46Z (GMT). No. of bitstreams: 1 ntu-100-R98b46011-1.pdf: 5757837 bytes, checksum: fa2450660f075149a82ba4b52873332a (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 章節目錄…………………………………………………………..……………..…… I
圖表目錄……………………………………………………………………………. III 中文摘要…………………………………………………………………….……… IV 英文摘要…………………………………………………………………………….. VI 第一章 序言 第一節、血管內皮細胞層……………………………………………….………… 1 第二節、血管發育與新生在病理學上的影響…………………….……………… 3 第三節、人類臍帶靜脈內皮細胞之蛋白質體學分析……………….…………… 4 第四節、血管生物標誌 ………………………………………………..….…… 5 第五節、基因轉殖血管螢光斑馬魚 Fli-1…………………………...…………… 7 第六節、研究目的及策略……………………………………………...……..…… 8 第二章 實驗材料與方法 第一節、實驗材料……………………………………………………………….… 9 第二節、實驗方法 一、斑馬魚的初代細胞培養………………………………...………………… 10 二、斑馬魚初代細胞的蛋白質體學分析……………………..………………. 12 三、目標基因之選殖……………………………………………...…………… 16 四、斑馬魚目標蛋白質之表現與純化…………………………..……………. 19 五、斑馬魚目標蛋白質之全覆式免疫染色……………………..……….…… 22 第三章 實驗結果 第一節、基因轉殖血管螢光斑馬魚初代細胞培養 一、從 wild type 斑馬魚胚胎的初代細胞培養建立餵養細胞…………….... 27 二、基因轉殖血管螢光斑馬魚 Fli-1:EGFP細胞與餵養細胞的共同培養..… 27 第二節、Fli-1:EGFP 血管螢光內皮細胞蛋白質體學分析 一、利用流式細胞分選儀篩選出 Fli-1:EGFP 血管螢光內皮細胞….……… 28 二、利用蛋白質體學分析不同發育階段血管內皮細胞之蛋白質表達型態... 30 第三節、斑馬魚 Thrombospondin 4A、4B 基因選質與序列分析 一、Thrombospondin 4A、4B基因選質與序列分析……………………...…… 31 二、利用大腸桿菌大量誘導 His-thrombospondin 4A、4B 融合抗原蛋白質的表現 ( Induction of recombinant protein ) ……………………………………….… 32 三、全覆性免疫螢光染色分析……………………………………..…….…… 33 第四章、實驗討論 第一節、斑馬魚胚胎幹細胞的驗證與應用…………………………..……….… 33 第二節、血管內皮細胞蛋白質體學分析比對…………………………..……… 35 第三節、血管內皮細胞新生物標誌的探尋……………………………..….…… 39 第五章、參考文獻…………………………………………………………...……… 44 第六章、備註……………………………………………………………………...… 55 | |
dc.language.iso | zh-TW | |
dc.title | 基因轉殖血管螢光斑馬魚蛋白質體學分析 | zh_TW |
dc.title | Proteomics analyses of Fli-1 transgenic zebrafish | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李明亭,張茂山 | |
dc.subject.keyword | 斑馬魚,血管內皮細胞,生物標誌, | zh_TW |
dc.subject.keyword | zebrafish,vascular endothelial cells,biomarker, | en |
dc.relation.page | 80 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-07-07 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科學研究所 | zh_TW |
顯示於系所單位: | 生化科學研究所 |
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