請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47802完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃鵬鵬(Pung-Pung Hwang) | |
| dc.contributor.author | Fang-Yu Liu | en |
| dc.contributor.author | 劉芳宇 | zh_TW |
| dc.date.accessioned | 2021-06-15T06:19:26Z | - |
| dc.date.available | 2011-01-01 | |
| dc.date.copyright | 2010-08-27 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-08-10 | |
| dc.identifier.citation | Blasiole, B., Canfield, V., Degrave, A., Thisse, C., Thisse, B., Rajarao, J., and Levenson, R. (2002). Cloning, mapping, and developmental expression of a sixth zebrafish Na, K-ATPase α1 subunit gene atp1a1a. 5. Mech. Dev. 119, S211-S214.
Bystriansky, J., Richards, J., Schulte, P., and Ballantyne, J. (2006). Reciprocal expression of gill Na+/K+-ATPase α-subunit isoforms α1a and α1b during seawater acclimation of three salmonid fishes that vary in their salinity tolerance. J. Exp. Biol. 209, 1848. Canfield, V.A., Loppin, B., Thisse, B., Thisse, C., Postlethwait, J.H., Mohideen, M.A., Rajarao, S.J., and Levenson, R. (2002). Na,K-ATPase alpha and beta subunit genes exhibit unique expression patterns during zebrafish embryogenesis. Mech. Dev. 116, 51-59. Chang, I., Lee, T., Yang, C., Wei, Y., Chou, F., and Hwang, P. (2001). Morphology and function of gill mitochondria rich cells in fish acclimated to different environments. Physiol. Biol. Zool. 74, 111-119. Chang, I., Wei, Y., Chou, F., and Hwang, P. (2003). Stimulation of Cl- uptake and morphological changes in gill mitochondria rich cells in freshwater tilapia (Oreochromis mossambicus). Physiol. Biol. Zool. 76, 544-552. Chen, Y., Lu, F., and Hwang, P. (2003). Comparisons of calcium regulation in fish larvae. J. Exp. Zool. Part A 295, 127-135. Cheng, K., Levenson, R., and Robishaw, J. (2003). Functional genomic dissection of multimeric protein families in zebrafish. Dev. Dynam. 228, 555-567. Crambert, G., Hasler, U., Beggah, A., Yu, C., Modyanov, N., Horisberger, J., Lelievre, L., and Geering, K. (2000). Transport and pharmacological properties of nine different human Na, K-ATPase isozymes. J. Biol. Chem. 275, 1976. Ellertsdottir, E., Ganz, J., Durr, K., Loges, N., Biemar, F., Seifert, F., Ettl, A., Kramer-Zucker, A., Nitschke, R., and Driever, W. (2006). A mutation in the zebrafish Na, K-ATPase subunit atp1a1a. 1 provides genetic evidence that the sodium potassium pump contributes to left-right asymmetry downstream or in parallel to nodal flow. Dev. Dynam. 235, 1794-1808. Esaki, M., Hoshijima, K., Kobayashi, S., Fukuda, H., Kawakami, K., and Hirose, S. (2007). Visualization in zebrafish larvae of Na+ uptake in mitochondria-rich cells whose differentiation is dependent on foxi3a. Am. J. Physiol. Integr. Comp. Physiol. 292, R470. Evans, D., Piermarini, P., and Choe, K. (2005). The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol. Rev. 85, 97. Feng, S., Leu, J., Yang, C., Fang, M., Huang, C., and Hwang, P. (2002). Gene expression of Na+-K+-ATPase alpha 1 and alpha 3 subunits in gills of the teleost Oreochromis mossambicus, adapted to different environmental salinities. Marine biotech. (New York, NY) 4, 379. Flik, G., Kaneko, T., Greco, A., Li, J., and Fenwick, J. (1997). Sodium dependent ion transporters in trout gills. Fish Physiol. Biochem. 17, 385-396. Hirata, T., Kaneko, T., Ono, T., Nakazato, T., Furukawa, N., Hasegawa, S., Wakabayashi, S., Shigekawa, M., Chang, M., and Romero, M. (2003). Mechanism of acid adaptation of a fish living in a pH 3.5 lake. Am. J. Physiol. Integr. Comp. Physiol. 284, 1199. Hoenderop, J., Nilius, B., and Bindels, R. (2005). Calcium absorption across epithelia. Physiol. Rev. 85, 373. Horng, J., Lin, L., Huang, C., Katoh, F., Kaneko, T., and Hwang, P. (2007). Knockdown of V-ATPase subunit A (atp6v1a) impairs acid secretion and ion balance in zebrafish (Danio rerio). Am. J. Physiol. Integr. Comp. Physiol. 292, R2068. Hwang, P. (2009). Ion uptake and acid secretion in zebrafish (Danio rerio). J. Exp. Biol. 212, 1745. Hwang, P., and Lee, T. (2007). New insights into fish ion regulation and mitochondrion-rich cells. Comp. Biochem. Physiol. Part A 148, 479-497. James, P., Grupp, I., Grupp, G., Woo, A., Askew, G., Croyle, M., Walsh, R., and Lingrel, J. (1999). Identification of a Specific Role for the Na, K-ATPase α2 Isoform as a Regulator of Calcium in the Heart. Mol. cell 3, 555-563. Jewell, E., and Lingrel, J. (1991). Comparison of the substrate dependence properties of the rat Na, K-ATPase alpha 1, alpha 2, and alpha 3 isoforms expressed in HeLa cells. J. Biol. Chem. 266, 16925. Lang, F., Capasso, G., Schwab, M., and Waldegger, S. (2005). Renal tubular transport and the genetic basis of hypertensive disease. Clin. Exp. Nephrol. 9, 91-99. Lee, T., Tsai, J., Fang, M., Yu, M., and Hwang, P. (1998). Isoform expression of Na+-K+-ATPase alpha-subunit in gills of the teleost Oreochromis mossambicus. Am. J. Physiol. Integr. Comp. Physiol. 275, 926. Liao, B., Chen, R., and Hwang, P. (2009). Expression regulation of Na+-K+-ATPase α 1-subunit subtypes in zebrafish gill ionocytes. Am. J. Physiol. Integr. Comp. Physiol. 296, R1897. Liao, B., Deng, A., Chen, S., Chou, M., and Hwang, P. (2007). Expression and water calcium dependence of calcium transporter isoforms in zebrafish gill mitochondrion-rich cells. BMC genomics 8, 354. Lin, C., and Lee, T. (2005). Sodium or potassium ions activate different kinetics of gill Na, K-ATPase in three seawater-and freshwater-acclimated euryhaline teleosts. J. Exp. Zool. Part A 303, 57-65. Lin, L., Horng, J., Kunkel, J., and Hwang, P. (2006). Proton pump-rich cell secretes acid in skin of zebrafish larvae. Am. J. Physiol. Cell Physiol. 290, C371. Lin, T., Liao, B., Horng, J., Yan, J., Hsiao, C., and Hwang, P. (2008). Carbonic anhydrase 2-like a and 15a are involved in acid-base regulation and Na+ uptake in zebrafish H+-ATPase-rich cells. Am. J. Physiol. Cell Physiol. 294, C1250. McCormick, S., Regish, A., and Christensen, A. (2009). Distinct freshwater and seawater isoforms of Na+/K+-ATPase in gill chloride cells of Atlantic salmon. J. Exp. Biol. 212, 3994. Miyazaki, H., Kaneko, T., Uchida, S., Sasaki, S., and Takei, Y. (2002). Kidney-specific chloride channel, OmClC-K, predominantly expressed in the diluting segment of freshwater-adapted tilapia kidney. Proc. Natl. Acad. Sci. USA 99, 15782. Mobasheri, A., Avila, J., Cozar-Castellano, I., Brownleader, M., Trevan, M., Francis, M., Lamb, J., and Martin-Vasallo, P. (2000). Na+, K+-ATPase isozyme diversity; comparative biochemistry and physiological implications of novel functional interactions. Bioscience reports 20, 51-91. Pagliarani, A., Ventrella, V., Ballestrazzi, R., Trombetti, F., Pirini, M., and Trigari, G. (1991). Salinity-dependence of the properties of gill (Na++ K+)-ATPase in rainbow trout (Oncorhynchus mykiss). Comp. Biochem. Physiol. Part B 100, 229-236. Pan, T., Liao, B., Huang, C., Lin, L., and Hwang, P. (2005). Epithelial Ca2+ channel expression and Ca2+ uptake in developing zebrafish. Am. J. Physiol. Integr. Comp. Physiol. 289, R1202. Parks, S., Tresguerres, M., and Goss, G. (2007). Interactions between Na+ channels and Na+-HCO3- cotransporters in the freshwater fish gill MR cell: a model for transepithelial Na+ uptake. Am. J. Physiol. Cell Physiol. 292, C935. Purkerson, J., and Schwartz, G. (2006). The role of carbonic anhydrases in renal physiology. Kidney Int. 71, 103-115. Reilly, R., and Ellison, D. (2000). Mammalian distal tubule: physiology, pathophysiology, and molecular anatomy. Physiol. Rev. 80, 277. Richards, J., Semple, J., Bystriansky, J., and Schulte, P. (2003). Na+/K+-ATPase α-isoform switching in gills of rainbow trout (Oncorhynchus mykiss) during salinity transfer. J. Exp. Biol. 206, 4475. Tang, C., Hwang, L., and Lee, T. (2010). Chloride channel ClC-3 in gills of the euryhaline teleost, Tetraodon nigroviridis: expression, localization and the possible role of chloride absorption. J. Exp. Biol. 213, 683. Wang, Y., Tseng, Y., Yan, J., Hiroi, J., and Hwang, P. (2009). Role of SLC12A10. 2, a Na-Cl cotransporter-like protein, in a Cl uptake mechanism in zebrafish (Danio rerio). Am. J. Physiol. Integr. Comp. Physiol. 296, R1650. Yan, J., Chou, M., Kaneko, T., and Hwang, P. (2007). Gene expression of Na+/H+ exchanger in zebrafish H+-ATPase-rich cells during acclimation to low-Na+ and acidic environments. Am. J. Physiol. Cell Physiol.293, C1814. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47802 | - |
| dc.description.abstract | 魚類的鰓與皮膚上的離子細胞對於維持體內離子平衡與酸鹼平衡扮演著一個非常重要的角色。先前的研究中在斑馬魚(Danio rerio)上發現了三種離子細胞分別為富含鈉鉀幫浦細胞(NaR cells)、富含氫離子幫浦細胞 (HR cells)，以及鈉氯離子運輸細胞(NCC cells)。這三種離子細胞分別負責鈣離子吸收、鈉離子吸收及酸的排出，和氯離子吸收。研究報告指出鈉鉀幫浦其次單元 (NKA subunit) 有三種同功異構物atp1a1a.1, atp1a1a.2, 以及 atp1a1a.5 分別表現在富含鈉鉀幫浦細胞、鈉氯離子運輸細胞，以及富含氫離子幫浦細胞。本實驗的主要目的是測試上述的三種同功異構物是否對斑馬魚上的三種離子細胞扮演著重要的角色。
本實驗主要利用基因專一反股核酸(morpholino-modified antisense oligonucleotide) 抑制上述三種的同功異構物的蛋白質表現。實驗結果發現當atp1a1a.1被抑制後鈣離子的流入有減少的情況，表示atp1a1a.1對於魚體鈣離子吸收有著一定的程度的影響。抑制atp1a1a.2後會造成氯離子在魚的整體量減少，同時發現鈉氯離子運輸蛋白的基因表現量降低，推測atp1a1a.2在鈉氯離子運輸細胞的電化學平衡扮演著重要的角色，使得鈉氯離子運輸蛋白無法正常的表現造成整體的氯離子含量下降。在atp1a1a.5的抑制實驗中發現鈉離子的整體量會下降，顯示atp1a1a.5對於富含氫離子幫浦細胞在鈉離子吸收的機制上是十分重要的。 綜合以上結果，本實驗首次提供在生物體(in vivo)層次上的分子生理證據，證明了不同的鈉鉀幫浦次單元同功異構物在斑馬魚皮膚及鰓上離子細胞的離子調控機制中所扮演的角色。 | zh_TW |
| dc.description.abstract | Gill and skin ionocytes are the major cell types which play an essential role in the transepithelial transport of ions and maintenance of the acid-base balance in fish. Previous studies found three subtypes of ionocytes in zebrafish (Danio rerio), Na+-K+ ATPase-Rich Cells (NaR cells), H+-ATPase-Rich Cells (HR cells) and Na+-Cl- Co-transporter Cells (NCC cells). These subtypes are responsible for Ca2+ uptake, Na+ uptake/acid secretion and Cl- uptake, respectively. A recent study on zebrafish has reported three distinct Na+-K+ ATPase (NKA) α1-subunit genes, atp1a1a.1, atp1a1a.2, and atp1a1a.5, which are expressed in NaR cells, NCC cells and HR cells, respectively.
The present study aims to test the hypothesis of whether the NKA α1 isoforms play distinct roles in the ion regulation pathways in the three types of ionocytes. Knockdown experiments with specific morpholinos against the three NKA α1 genes were conducted. The results showed that knockdown of atp1a1a.1 decreased the Ca2+ influx in the morphants. This indicates the role of atp1a1a.1 in Ca2+ uptake mechanism in NaR cells is important. Knockdown of atp1a1a.2 decreased the Cl- content and the mRNA expression of NCC in the morphants. These results suggest that loss-of-function of ATP1a1a.2 has may impact the intracellular electrochemistry and thus affect the transcription/translation of other transporters, consequently resulting in impairment to Cl- uptake function in NCC cells. Knockdown of atp1a1a.5 in zebrafish embryos showed a significant decrease of whole body Na+ content, implying an importance of ATP1a1a.5 in Na+ uptake mechanisms in HR cells. These results for the first time provide in vivo molecular physiological evidence for the different roles of NKA α1-subunit isoforms in zebrafish gill/skin ion regulation mechanisms. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T06:19:26Z (GMT). No. of bitstreams: 1 ntu-99-R97B45018-1.pdf: 1271918 bytes, checksum: e44fbf85974413ee66440cbcee759d00 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | Introduction………………………………….………………………. 1
Na+-K+-ATPase (NKA) alpha 1 subunit isoforms……….……… 1 Ion regulation in FW fish………..……………………………… 2 Fish gill and ionocytes………..……………….………………… 2 ATPase-rich (NaR) cells………….……..……………… 3 Na+-Cl- co-transporter (NCC) cells……….……..……………… 4 H+-ATPase-rich (HR) cells…………………..…..……………… 5 Purpose of the study….……………………………………………….. 8 Materials and Methods……………………………………………….. 9 Animal……….…………………………………...………………… 9 Morpholino oligonucleotides (MO) design and injection……...… 9 Measurement of whole body ion content……………………… 10 Measurement of whole body Ca2+ influx…………………..…… 10 Preparation of total RNA…………………...…………………… 11 RT-PCR analysis……………...………………………………… 11 Quantitative real-time PCR…...………………………………… 12 Immunocytochemistry…...……………………………………… 12 Statistical analysis…..…...……………………………………… 13 Results …………………………………………………………….. 14 Results of atp1a1a.1 knockdown zebrafish embryos…………… 14 Results of atp1a1a.2 knockdown zebrafish embryos…………… 16 Results of atp1a1a.5 knockdown zebrafish embryos…………… 16 Discussion …………………………………………………………… 18 References………………………………………………….….…… 25 Tables and figures…………………………………………..………. 33 | |
| dc.language.iso | en | |
| dc.subject | 鈉鉀幫浦 | zh_TW |
| dc.subject | 離子調控 | zh_TW |
| dc.subject | 斑馬魚 | zh_TW |
| dc.subject | ion regulation | en |
| dc.subject | Sodium pump | en |
| dc.subject | zebrafish | en |
| dc.title | 鈉鉀幫浦次單元在斑馬魚離子調控機制中的角色 | zh_TW |
| dc.title | Roles of Na-K ATPase α1-subunit isoforms in zebrafish ion regulation mechanism | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張清風,李宗翰,韓玉山 | |
| dc.subject.keyword | 鈉鉀幫浦,斑馬魚,離子調控, | zh_TW |
| dc.subject.keyword | Sodium pump,zebrafish,ion regulation, | en |
| dc.relation.page | 44 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-08-10 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-99-1.pdf 未授權公開取用 | 1.24 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。