請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47598完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 梁博煌 | |
| dc.contributor.author | Yu-Ping Weng | en |
| dc.contributor.author | 翁羽平 | zh_TW |
| dc.date.accessioned | 2021-06-15T06:07:54Z | - |
| dc.date.available | 2010-08-18 | |
| dc.date.copyright | 2010-08-18 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-08-13 | |
| dc.identifier.citation | REFERENCES
[1] Karatan E, Watnick P (June 2009). 'Signals, regulatory networks, and materials that build and break bacterial biofilms'. Microbiology and Molecular Biology Reviews : MMBR 73 (2): 310–47. [2] Hoffman LR, D'Argenio DA, MacCoss MJ, Zhang Z, Jones RA, Miller SI (August 2005). 'Aminoglycoside antibiotics induce bacterial biofilm formation'. Nature 436 (7054): 1171–5. [3] An D, Parsek MR (June 2007). 'The promise and peril of transcriptional profiling in biofilm communities'. Current Opinion in Microbiology 10 (3): 292–6. [4] 'Research on microbial biofilms (PA-03-047)'. NIH, National Heart, Lung, and Blood Institute. 2002-12-20. [5] Rogers A H (2008). Molecular Oral Microbiology. Caister Academic Press. [6] Imamura Y, Chandra J, Mukherjee PK, et al. (January 2008). 'Fusarium and Candida albicans biofilms on soft contact lenses: model development, influence of lens type, and susceptibility to lens care solutions'. Antimicrobial Agents and Chemotherapy 52 (1): 171–82. [7] Lewis K (April 2001). 'Riddle of biofilm resistance'. Antimicrobial Agents and Chemotherapy 45 (4): 999–1007. [8] Parsek MR, Singh PK (2003). 'Bacterial biofilms: an emerging link to disease pathogenesis'. Annual Review of Microbiology 57: 677–701. [9] Sanderson AR, Leid JG, Hunsaker D (July 2006). 'Bacterial biofilms on the sinus mucosa of human subjects with chronic rhinosinusitis'. The Laryngoscope 116 (7): 1121–6. [10] Hall-Stoodley L, Costerton JW, Stoodley P (February 2004). 'Bacterial biofilms: from the natural environment to infectious diseases'. Nature Reviews. Microbiology 2 (2): 95–108. [11] Looking for Chinks in the Armor of Bacterial Biofilms Monroe D PLoS Biology Vol. 5, No. 11. [12] Coserton, M. P., Lewandowski, Z., Caldwell, D. E., Korber, D.R., and Lappin-Scott, H.M. (1995) Microbial biofilms. Annu. Rev. Microbiol. 49, 711-745. [13] Dunny, G. M., and Leonard, B. A. (1997) Cell-cell communication in gram-positive bacteria. Annu. Rev. Microbiol. 51,527-564. [14] Fugua, C., Winans, S. C., and Greenberg, E. P. (1996) Census and consensus in bacterial ecosystems: The LuxR-LuxI Family of Quorum-Sensing Transcriptional Regulators. Annu. Rev. Microbiol. 50, 727-751 [15] Kleerebezem, M., Quadri, L. E. N., Kuipers, O. P., and deVos, W. M. (1997) Quorum sensing by peptide pheromones and two-component signal-transduction systems in Gram-positive bacteria. Mol. Microbiol. 24, 895-904. [16] Miller, M. B., and Bassler, B. L. (2001) Quorum sensing in bacteria. Annu. Rev. Microbiol. 55, 165-199. [17] Chen, X., Shauder, S., Potier, N., Van Dorsselaer, A., Pelczer, I., Bassler, B. L., and Hughson. F. M. (2002) Structural identification of a bacterial quorum-sensing signal containing boron. Nature 415, 545-549. [18] Pesci, E. C., Milbank, J. B., Pearson, J. P., McKnight, S., Kende, A. S., Greenberg, E. P., and Iglewski, B. H. (1999) Quinolone signaling in the cell-to-cell communication system of Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. 96, 11229-11234. [19] Schauder, S., Shokat, K., Surette, M. G., and Bassler, B. L. (2001) The LuxS family of bacterial autoinducers: biosynthesis of a novel quorum-sensing signal molecule. Mol. Microbiol. 41, 463-476. [20] Wang, D.m Ding, X., and Rather, P. N. (2001) Indole can act as an extracellular signal in Escherichia coli. J. Bacteriol. 183, 4210-4216. [21] Sturgill, G., Toutain, C. M., Komperda, J., O’toole, G. A., and Rather, P. N. (2004) Role of cysE in production of an extracellular signaling molecule in Providencia stuartii and Escherichia coli: Loss of cysE Enhances Biofilm Formation in Escherichia coli. J. Bacteriol. 186, 7610-7617. [22] Kawamura-Sato K, Shibayama K, Horii T, Iimuma Y, Arakawa Y, Ohta M. (1999) Role of multiple efflux pumps in Escherichia coli in indole expulsion. FEMS Microbiol Lett. 179,345-52. [23] Vuong, C., Kocianova, S., Voyich, J. M., Yao, Y., Fisher, E. R., DeLeo, F. R., and Otto, M. (2004). A crucial role for exopolysaccharide modification in bacterial biofilm formation, immune evasion, and virulence. J. Biol. Chem. 279, 54881-54886. [24] Nobuhisa Masuda and Church, G. M.(2003) Regulatory network of acid resistance genes in Escherichia coli. Mol. Microbiol. 48, 699-712 [25] Haitian Liu, ThomasK. Ritter, Reiko Sadamoto, Pamela S. Sears, Min Wu, and Chi-Huey Wong (2003). Acceptor Specificity and Inhibition of the Bacterial Cell-Wall Glycosyltransferase MurG. ChemBioChem, 4, 603–609. [26] Christiane Gerke, Angelika Kraft, Roderich Su¨ ßmuth, Oliver Schweitzer,and Friedrich Go¨ tz (1998). Characterization of the N-Acetylglucosaminyltransferase Activity Involved in the Biosynthesis of the Staphylococcus epidermidis Polysaccharide Intercellular Adhesin. Vol. 273, No. 29, Issue of July 17, pp. 18586–18593. [27] Birgit M. Pru¨ß, Christopher Besemann, Anne Denton, and Alan J. Wolfe. A Complex Transcription Network Controls the Early Stages of Biofilm Development by Escherichia coli. JOURNAL OF BACTERIOLOGY, June 2006, p. 3731–3739. [28] Cynthia B. Whitchurch, Scott A. Beatson, James C. Comolli1, Thania Jakobsen, Jennifer L. Sargent, Jacob J. Bertrand, Joyce West, Mikke Klausen, Leslie L. Waite1, Pil Jung Kang,Tim Tolker-Nielsen, John S. Mattick, and Joanne N. Engel. Pseudomonas aeruginosa fimL regulates multiple virulence functions by intersecting with Vfr-modulated pathways, Mol Microbiol. 2005 March ; 55(5): 1357–1378. [29] Wen Chen, Kiyonobu Honma, Ashu Sharma & Howard K. Kuramitsu, (2006) Auniversal stress protein ofPorphyromonas gingivalis is involved in stress responses and bio¢lm formation, FEMS Microbiol Lett 264 15–21. [30] Kenneth F Blount1 & Ronald R Breaker, (2006) Riboswitches as antibacterial drug targets. DECEMBERNATURE BIOTECHNOLOGY. VOLUME 24 NUMBER 12 1558-1564. [31] Hidetada Hirakawa, Yoshihiko Inazumi, Takeshi Masaki, Takahiro Hirata and Akihito Yamaguchi, Indole induces the expression of multidrug exporter genes in Escherichia coli. Molecular Microbiology (2005) 55 (4), 1113–1126. [32] Kunihiko Nishino and Akihito Yamaguchi, EvgA of the Two-Component Signal Transduction System Modulates Production of the YhiUV Multidrug Transporter in Escherichia coli. JOURNAL OF BACTERIOLOGY, Apr. 2002, p. 2319–2323. [33] Elizabeth Burrowes, Christine Baysse, Claire Adams and Fergal O'Gara, Influence of the regulatory protein RsmA on cellular functions in Pseudomonas aeruginosa PAO1, as revealed by transcriptome analysis. Microbiology 152 (2006), 405-418. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47598 | - |
| dc.description.abstract | ABSTRACT
之前我們已經以二維膠片的分析技術發現當大腸桿菌的重要病原菌O157被處以抗生素枯草桿菌素時會經由EvgS/EvgA雙蛋白質系統引起Tryptophanase基因的表現，並進而分解Tryptophan引起Indole的產生，接著引起生物膜的形成以及一系列相關蛋白質的表達。 在這篇論文中我們以被跳躍子基因破壞的大腸桿菌DH5α突變株作為研究材料，進一步研究哪些基因為由Indole引起大腸桿菌形成生物膜所必須，結果發現當yhiP、cyaA、trkA、phr、fimE突變時大腸桿菌無法為Indole引起生物膜的形成。經由互補實驗我們也進一步證實這些基因確實為Indole引起大腸桿菌形成生物膜所必須。我們也利用報導基因分析再進一步證實Indole確實能引起這些基因的表現，其中又以yhiP、cyaA活性增加至200% 以上最為明顯﹔另外經由互補實驗和報導基因分析我們也部分的闡明了Indole與這些基因上游的轉錄調控子之間的關聯﹔以及各種常見抗生素對這些基因表現的影響。除此之外，在這篇論文中也以butanol等實驗材料分析了這些基因對大腸桿菌形成生物膜以對抗逆境的貢獻。 另外由於我們之前發現了在大腸桿菌中蛋白質B0363能為autoinducer (indole)引發表現且為金黃色葡萄球菌IcaA的類似物，因為它被預測是被分泌至細胞膜外側的部分水溶性蛋白質較易於分析，所以在這篇論文中我們也表達純化了這個蛋白質並做了相關的活性分析並且得到了高純度且具有活性的蛋白質。 | zh_TW |
| dc.description.abstract | ABSTRACT
By using the two-dimensional gel method, we already discovered that when E. coli O157 was exposed to bacitracin, the expression of tryptophanase gene was induced through the EvgS/EvgA two-component system. Tryptophanase then converted tryptophan to indole,which caused biofilm formation as well as a series of related protein expression. In this study, we screened the E. coli DH5α transposon library mutants to find out which genes are crucial for indole induced biofilm formation. These results show that when yhiP, cyaA, trkA, phr, and fimE genes are disrupted by transposon, indole can not induce these mutant strains to form biofilm. By using complementary assays, we confirmed that these genes are truly necessary for Indole induced biofilm formation in E. coli DH5α. Besides, we also used the reporter gene analysis method to further confirm that indole can cause these genes expressions with the promoter activity of yhiP, cyaA increased up to 200%. Moreover, we also clarified the relationship between indole and expressions of some transcription factor genes upstream to these genes, as well as the influences of each kind of common antibiotic to the expressions of these genes. In addition, we also used other experiment material such as butanol to test if expressions of these genes also contribute to the resistence to the adverse circumstance when E. coli formed biofilm. Besides, the expression of B0363 can be induced byindole in E.coli. B0363 is an analogous protein of IcaA of staphylococcus, which is secreted to outside of cell after transcription. I also expressed and purified this protein successfully and made sure it has catalytic activity. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T06:07:54Z (GMT). No. of bitstreams: 1 ntu-99-R94b46027-1.pdf: 1053858 bytes, checksum: c86c28bef4497690475dee2341caa379 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | TABLE OF CONTENTS
ABSTRACT……………………………………………………………3 ABBREVIATIONS…………………………………………………… 5 INTRODUCTION………………………………………………………6 MATERIALS AND METHODS………………………………………… 8 RESULTS……………………………………………………………17 DISCUSSION……………………………………………………… 22 TABLES…………………………………………………………… 27 FIGURES……………………………………………………………31 REFERENCES……………………………………………………… 63 APPENDICES……………………………………………………… 68 | |
| dc.language.iso | zh-TW | |
| dc.title | 在大腸桿菌DH5α中由吲哚引起生物膜形成所必需的蛋白質及其功能 | zh_TW |
| dc.title | The proteins needed for indole induced biofilm formation and their functions in E. coli DH5α | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張震東,朱善德 | |
| dc.subject.keyword | 吲,哚,生物膜, | zh_TW |
| dc.subject.keyword | indole,biofilm, | en |
| dc.relation.page | 70 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-08-15 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-99-1.pdf 目前未授權公開取用 | 1.03 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。