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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 謝文陽 | |
| dc.contributor.author | Hao-Han Chang | en |
| dc.contributor.author | 張皓涵 | zh_TW |
| dc.date.accessioned | 2021-06-15T06:05:16Z | - |
| dc.date.available | 2010-09-15 | |
| dc.date.copyright | 2010-08-19 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-08-15 | |
| dc.identifier.citation | 古森本(2008). 生質能源作物之開發與潛力. 農業生技產業季刊。植物種苗生技 13:46-53.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47546 | - |
| dc.description.abstract | 本研究於2009年8月間，在台灣北部沿海採集四個樣點的水樣，利用GPYS-TC固態培養基加以進行分離、純化後，一共分離出二十六株酵母菌，將分離株的5.8S–ITS rDNA 區域片段定序，並藉由National Center for Biotechnology Information (NCBI)網站內序列比對，結果顯示二十六株酵母菌分別屬於Pichia, Candida, Cryptococcus, Rhodotorula, Issatchenkia五個屬，進一步探討分離株的生長特性、生理特性、菌落的形態以及26S rDNA D1/D2區域片段序列後，初步判定B4、C2、D1與D7分別為Cryptococcus liquefaciens、Cryptococcus flavus、Issatchenkia orientalis、Rhodotorula mucilaginosa，而D17、A4、A5、A1、D3、B2與C3七株分離株則可能為新種酵母菌；另外，各群代表株均具有葡萄糖發酵產乙醇的能力，其中，分離株D3在GY100培養基培養60小時有最高乙醇產率25.3 g/L，但在24小時，有每小時最高乙醇轉換率0.72%。 | zh_TW |
| dc.description.abstract | Twenty six yeast strains were isolated from four sampling sites of north Taiwan in August ,2009. According to 5.8S–ITS rDNA sequence analyses by NCBI GeneBank, these strains could be divided into nine genotypes. The results indicate that these twenty six yeast strains could be belonged to Pichia, Candida, Cryptococcus, Rhodotorula and Issatchenkia, respectively. In polyphasic studies, such as physiological and biochemical characteristics and 26S rDNA D1/D2 sequence analyses, these four representative strains, B4, C2, D1and D7 were blonged to Cryptococcus liquefaciens, Cryptococcus flavus, Issatchenkia orientalis and Rhodotorula mucilaginosa, respectively. D17, A4, A5, A1, D3, B2 and C3 should be classified into novel species. D3, the highest ethanol production efficiency strain, could produce 25.3 g/L ethanol in GY100 broth in 60 hours. In 24 hours, the highest ethanol conversion rate of D3 was 0.72% per hour. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T06:05:16Z (GMT). No. of bitstreams: 1 ntu-99-R97241212-1.pdf: 1536930 bytes, checksum: 1ec9ca758159a05c29ac06061c83f34d (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 第一章 前言 1
一、酵母菌 1 二、環境變遷，替代能源興起 2 第二章 材料和方法 6 第一節、海水樣本採集 6 第二節、酵母菌分離與純化培養基配製 6 第三節、分子生物學鑑定 6 第四節、形態學鑑定 8 第五節、粗蛋白與粗脂肪分析 9 第六節、菌株生理與生化試驗 10 第三章 結果 16 第一節、酵母菌之分離 16 第二節、酵母菌株之5.8S - ITS rDNA 基因序列分析 16 第三節、分離株之形態與細胞組成成份分析 16 第四節、菌株生理與生化分析 19 第五節、各群代表株26S rDNA D1/D2基因序列分析 21 第六節、測定乙醇濃度 22 第四章、討論 24 第一節、海洋酵母菌 24 第二節、依據分子生物學、菌落形態與生理生化測試進行菌株鑑定 24 第三節、與分群方法代表株選定 28 第四節、發酵產乙醇能力 28 五、結論 30 參考文獻 31 | |
| dc.language.iso | zh-TW | |
| dc.title | 台灣北部沿海酵母菌之分離及特性研究 | zh_TW |
| dc.title | Isolation and characterization of yeasts in coastal seawaters of north Taiwan | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳卓昇,劉玫英,陳瑞芬,呂誌翼 | |
| dc.subject.keyword | 酵母菌,畢赤酵母屬,念珠菌屬,隱球菌屬,紅酵母屬,依薩酵母屬,乙醇轉換率, | zh_TW |
| dc.subject.keyword | yeast,Pichia,Candida,Cryptococcus,Rhodotorula,Issatchenkia,ethanol conversion rate, | en |
| dc.relation.page | 79 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-08-16 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 海洋研究所 | zh_TW |
| 顯示於系所單位： | 海洋研究所 | |
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