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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝松蒼 | |
dc.contributor.author | Chi-Ming Pu | en |
dc.contributor.author | 蒲啟明 | zh_TW |
dc.date.accessioned | 2021-06-15T06:02:58Z | - |
dc.date.available | 2011-09-09 | |
dc.date.copyright | 2010-09-09 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-16 | |
dc.identifier.citation | Brenner MJ, Hess JR, et al. (2006) Repair of motor nerve gaps with sensory nerve inhibits regeneration in rats. Laryngoscope 116(9):1685-92
Brown MC, Hopkins WG, et al. (1981) Role of degenerating axon pathways in regeneration of mouse soleus motor axons. J Physiol 318:365–73 Brown MC, Lunn ER, et al. (1992) Consequences of slow Wallerian degeneration for regenerating motor and sensory axons. J Neurobiology 23(5):521-36 Brown MC, Perry VH, et al. (1994) Further studies on motor and sensory nerve regeneration in mice with delayed Wallerian degeneration. Euro J Neurosci 6:420-28 Dyck PJ , et al. (1993) Peripheral neuropathy 3rd edition Philadelphia, PA : W.B. Saunders Gutmann BE, Guttmann L, et al. (1941) The rate of regeneration of nerve. Department of Zoology and Comparative Anatomy; Oxford 14-44 Hsieh YL, Lin WM, et al. (2009) Effects of 4-Methylcatechol on skin reinnervation: promotion of cutaneous nerve regeneration after crush injury. J Neuropathol Exp Neurol 68(12):1269-81 Hoke A, Redett R, et al. (2006) Schwann cells express motor and sensory phenotypes that regulate axon regeneration. J Neurosci 26(38):9646 –55 Jenq CB, Coggeshall RE, et al. (1985) Numbers of regenerating axons in parent and tributary peripheral nerves in the rat. Brain Res 326:27–40 Kawamura DH, Johnson PJ, et al. (2010) Matching of motor-sensory modality in the rodent femoral nerve model shows no enhanced effect on peripheral nerve regeneration. Exp Neurol 223(2):496-504 Kirsch M, Friz MC, et al. (2009) Wallerian degeneration and axonal regeneration after sciatic nerve crush are altered in ICAM-1-deficient mice. Cell Tissue Res 338(1):19-28 Krurup C, Loeb GE, et al. (1988) Conduction studies in peripheral cat nerve using implanted electrodes :II. The effects of prolonged constriction of prolonged construction on regeneration of crushed nerve fibers. Muscle and Nerve 11:933-44 Lago N, Navarro X, et al. (2006) Correlation between target reinnervation and distribution of motor axons in the injured rat sciatic nerve. J Neurotrauma 23(2):227-40 Madison RD, Archibald SJ, et al. (1996) Reinnervation accuracy of the rat femoral nerve by motor and sensory neurons. J Neurosci 16(18):5698–5703 Moradzadeh A, Borschel GH, et al. (2008) The impact of motor and sensory nerve architecture on nerve regeneration. Exp Neurol 212:370–76 Navarro X, Verdu E, t al. (1994) Comparison of regenerative and reinnervating capabilities of different functional types of nerve fibers. Exp Neurol 129:217-24 Navarro X, Vivo` M, et al. (2007) Neural plasticity after peripheral nerve injury and regeneration. Prog Neurobiol 82:163-201 Negredo P, Castro J, et al. (2004) Differential growth of axons from sensory and motor neurons through a regenerative electrode: A stereological, retrograde tracer, and functional study in the rat. Neuroscience 128:605-15 Neubauer D, Graham JB, et al. (2010) Nerve grafts with various sensory and motor fiber compositions are equally effective for the repair of a mixed nerve defect. Exp Neurol 223(1):203-6 Nichols CM, Brenner MJ, (2004) Effect of motor versus sensory nerve grafts on peripheral nerve regeneration. Exp Neurol 190 :347– 55 Schmalbruch H, (1986) Fiber composition of the rat sciatic nerve. Anatomical Record 215:71-81 Suzuki GI, Ochi M, et al. (1998) Sensory neurons regenerate more dominantly than motoneurons during the initial stage of the regenerating process after peripheral axotomy. NeuroReport 9:3487-91 Verdu E, Navarro X (1997) Comparison of immunohistochemical and functional reinnervation of skin and muscle after peripheral nerve injury. Exp Neurol 146: 187–98 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47498 | - |
dc.description.abstract | 當周邊神經受傷時,軸索(axon)被切斷時,在軸索的遠端會進行一種陳規性的退化現象,稱為瓦勒氏退化(Wallerian degeneration),此退化現象將提供適當的細胞及分子環境做為神經再生的環境。但是對於有神經缺損(尤其是運動神經缺損)的周邊神經損傷來說,無法以直接端對端的方法直接縫合,神經移植(nerve graft)則是一個標準的手術方法。不同種類的神經再生速度也不盡相同。許多研究報告指出:運動神經和感覺神經的再生速度存在著差異,但是何種神經有較快的再生速度仍有許多爭議。本實驗的目的是希望利用坐骨神經截斷的老鼠,以自體神經原位移植(orthotopically re-implantation)的方式將一小段神經(6mm)縫回原來截斷的位置,在不同的時間點(術後一週、一、三及六個月)以型態學的方法觀察脛神經和腓腸神經中具髓鞘神經軸索恢復的密度和軸索直徑大小的變化來比較運動神經和感覺神經的再生速度。本研究中,我們發現:(1)脛神經內再生之具髓鞘神經軸索數目和腓腸神經相等。(2)神經移植使得再生神經軸索直徑變小(shift to left),數目也比原來少,功能的恢復會比壓傷或截斷傷直接縫合不好。(3)脛神經與腓腸神經增生的神經軸索在神經受傷的三個月內即增生完成。由於脛神經屬於混合型神經(mix nerve),而腓腸神經為純感覺神經(sensory nerve),之前學者推論脛神經內運動神經軸索的比例應該小於全部軸索30% (Schmalbruch , 1986; Brown et al., 1992),根據我們的實驗,在六個月時脛神經再生軸索恢復的比例為1.67±0.28,腓腸神經為1.21±0.19,推算出運動神經:感覺神經的再生神經軸索為0.67:0.2,因此我們初步認為運動神經的再生效率比感覺神經快速;如此的推論仍需更進一步的實驗驗證。 | zh_TW |
dc.description.abstract | Peripheral nerves have inherent potential for regeneration because of the ideal microenvironment for axonal regeneration provided by the distal nerve stump after Wallerian degeneration. If primary repair is not possible when the nerve is transected, clinical use of autologous nerve grafts has become the gold standard for repairing the injured nerve. The recovery of function after nerve graft not only depends on types of nerve graft but also depends on types of injured nerves. Sensory or motor nerve inherents better nerve regeneration efficiency still exists debates in previous literatures. In the present study, we use morphometric analysis to compare the myelinated nerve regeneration efficiency between tibia nerve and sural nerve in sciatic nerve graft mice. There are significant difference in regenerated axon number and density ratio between post operative seven days (POD7), one (POM1) and three (POM3) months in both tibia and sural nerve (p<0.05). In post operative six months (POM6), the regeneration axon density ratio is higher in tibia nerve than in sural nerve (p<0.05). The histogram of myelinated axon fiber in POM3 and POM6 showed a shift-to-left phenomenon in both tibia and sural nerves. These datas indicate regenerated myelinated axon number and density increased from POD7 to POM3 but maintained from POM3 to POM6. The size of regenerated axon is smaller than control side in POM3 and POM6 mice. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T06:02:58Z (GMT). No. of bitstreams: 1 ntu-99-R96446001-1.pdf: 2513228 bytes, checksum: a1ae35040847e7113a537805db506826 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 謝辭 .....................................................i
中文摘要 .................................................ii 英文摘要.................................................iii 圖目錄 ...................................................3 表目錄 ...................................................4 第一章 引言 .............................................5 1.1 瓦勒氏退化與神經再生 .............................5 1.2 神經移植 .........................................5 1.3 神經損傷種類 .....................................6 1.4 感覺神經與運動神經再生速度之差異 .................7 1.5 研究目的 .........................................7 第二章 實驗材料與方法 ...................................8 2.1 動物飼養 .........................................8 2.2 動物手術 .........................................8 2.3 脛神經及腓腸神經厚片切片之觀察 ...................8 2.4 統計分析 .........................................9 第三章 實驗結果 ........................................10 3.1脛神經及腓腸神經厚片切片之觀察 ..................10 3.2各個不同時間點,比較實驗側脛神經及腓腸神經再生神經軸索數目、神經纖維面積及神經軸索密度與對照側之比值差異 ....................................10 3.3再生神經軸索數目比值比較 ........................11 3.4神經纖維面積比值比較 ............................11 3.5再生神經軸索密度比值比較 ........................11 3.6再生神經軸索直徑大小變化 ........................12 第四章 討論 ............................................13 4.1比較各個不同時間點,實驗側脛神經及腓腸神經再生神經軸索數目、神經纖維面積及神經軸索密度與對照側之比值差異 ....................................13 4.2再生神經軸索數目比值比較 ........................14 4.3再生神經軸索密度比值比較 ........................14 4.4脛神經具髓鞘神經軸索數量 ........................15 4.5結論 ............................................15 第五章 參考文獻 ........................................17 附錄 ....................................................20 表格 圖片及圖片說明 | |
dc.language.iso | zh-TW | |
dc.title | 坐骨神經移植老鼠其具髓鞘神經生長速率之比較 | zh_TW |
dc.title | Comparison of Myelinated Nerve Regeneration Efficiency after Sciatic Nerve Graft in Mice | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 簡雄飛,謝侑霖 | |
dc.subject.keyword | 具髓鞘神經軸索再生效率,周邊神經損傷,瓦勒氏退化,神經移植,感覺神經,運動神經, | zh_TW |
dc.subject.keyword | regenerated myelinated axon efficiency,peripheral nerve injury,Wallerian degeneration,nerve graft,motor nerve,sensory nerve, | en |
dc.relation.page | 46 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-08-17 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 解剖學暨生物細胞學研究所 | zh_TW |
顯示於系所單位: | 解剖學暨細胞生物學科所 |
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