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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林達德 | |
dc.contributor.author | Yu-Chou Chen | en |
dc.contributor.author | 陳俞州 | zh_TW |
dc.date.accessioned | 2021-06-15T06:00:01Z | - |
dc.date.available | 2011-08-22 | |
dc.date.copyright | 2011-08-22 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-19 | |
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A fluorescence optic-fiber temperature sensor using phase-locked detection with pulse modulation single reference. Journal of Physics. 48: 101-105. 53. Wuister, S. F., C. M. Donega, and A. Meijerink. 2004. Luminescence temperature antiquenching of water-soluble CdTe quantum dots: role of the solvent. Journal of the American Chemical Society. 126(33): 10397-10402. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47442 | - |
dc.description.abstract | 溫度的量測一直是被重視的問題,近代甚至發展出以光學原理檢測溫度之技術,螢光測溫技術即為其中之一分支。利用螢光來量測溫度可以克服過去被量測物處於高電壓、高磁場環境下,一般溫度感測器無法順利量測到之情形。本研究應用螢光影像之方法來量測溫度,主要先藉由熱電致冷式低溫顯微鏡來模擬環境溫度,並利用低溫顯微鏡可即時控制溫度之功能,達到快速且即時模擬溫度之需求。研究中使用內含螢光溶液之微膠囊做為實驗樣本,並對其進行激發,然後觀察其在不同溫度下之螢光影像強度。實驗內容主要分成兩大部份,第一部份為分別對不同環境變因進行螢光微膠囊的實驗,以探討螢光在不同濃度與溶劑之激發情形,並進行不同螢光微膠囊大小與激發光源距離的螢光實驗,最後利用這些數據找出適合後續實驗之條件。第二部份則是對螢光影像強度進行溫度的量測,方法是利用所獲得之螢光影像經分析後建立的螢光量測模型,以此模型對螢光微膠囊所在之環境溫度進行量測,可量測溫度範圍為介於-15~ 90 ℃之間。本研究建立之螢光量測模型所使用之方法包含螢光隨時間衰減法、單一螢光強度法及螢光比值法,其均方根誤差分別為9.74 ℃、5.04 ℃及3.41 ℃;且在三次不同實驗中所得之迴歸判定係數分別為0.890、0.979及0.992。實驗結果顯示,利用螢光微膠囊所獲得之螢光影像在不同環境溫度下其強度有不同之變化,因此可應用於溫度的量測;而所建立的螢光量測模型中,使用螢光比值法有較佳的結果,實驗的重複性也較其他方法來得好。 | zh_TW |
dc.description.abstract | Measuring temperature is a very important issue. Recently, using optical technologies to measure temperature has been developed, such as fluorescent imaging. This method can overcome the problem that objects under the situation of high voltage or high magnetic field, which normal thermometer can’t apply. In this research we using fluorescent imaging technology to measure temperature. The main idea is using thermoelectric cooler cryomicroscope which can control temperature easily to simulates temperature we needed. The samples in this research are fluorescent microcapsules, then excited it by blue LED and observed intensity of fluorescent image in different temperature. This research can be divided into two parts. The first part is to investigate the effect of different conditions to fluorescent microcapsules, such as size of microcapsules, distance of excited light, concentration of fluorescent solution. The second part is to establish measurement models by experimental results, then using the models to measure the temperature. The measurable range is -15 ~ 90 ℃. The models in this research are based on method of fluorescent decay, method of single fluorescent intensity, and method of fluorescent ratio. Each method has best RMSE = 9.74 ℃, 5.04 ℃, and 3.41 ℃. And R2 = 0.890, 0.979, and 0.972. The results show fluorescent imaging technology can really apply to temperature measurement, also the best model is method of fluorescent ratio, and it more repeatable than other methods. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T06:00:01Z (GMT). No. of bitstreams: 1 ntu-100-R98631035-1.pdf: 2993512 bytes, checksum: 9e0544d31e854029aa52c4795a032e24 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 誌謝 i
摘要 ii ABSTRACT iii 目錄 iv 第一章 緒論 1 1.1前言 1 1.2研究目的 2 第二章 文獻探討 3 2.1溫度感測器 3 2.1.1熱電效應溫度感測器 3 2.1.2光纖傳感器 4 2.1.3螢光之溫度量測技術 5 2.1.4溫度感測器之比較 6 2.2螢光應用於溫度量測 7 2.2.1溫度改變抑制螢光發光之機制 7 2.2.2溫度改變抑制螢光衰減速度之機制 9 2.2.3螢光量測溫度之應用 11 2.3低溫顯微鏡 14 2.3.1低溫顯微鏡之類型 14 2.3.2熱電致冷式低溫顯微鏡 16 2.3.3低溫顯微鏡之討論與應用 17 2.4微膠囊(Microcapsules) 18 2.4.1微膠囊簡介 18 2.4.2微膠囊製作 19 第三章 研究設備與方法 20 3.1實驗設備 20 3.1.1整體系統架構 20 3.1.2冷凍台系統 21 3.1.3散熱系統 23 3.1.4溫度控制系統 23 3.1.5光源控制系統 25 3.1.6螢光影像擷取系統 27 3.1.7可溫控與燈控之影像擷取軟體 28 3.2螢光與激發光光譜 30 3.2.1螢光選擇 30 3.2.2激發光與螢光光譜 32 3.3樣本準備 36 3.3.1微膠囊製作方法 36 3.3.2微膠囊尺寸篩選與量測方法 38 3.3.3微膠囊製作變因與螢光微膠囊 39 3.4研究方法 40 3.4.1溫度校正 40 3.4.2螢光影像之處理 41 3.4.3溫度模型之建立與量測 43 第四章 結果與討論 44 4.1微膠囊與螢光微膠囊 44 4.1.1微膠囊之大小統計 44 4.1.2螢光微膠囊之顯微影像 48 4.2螢光微膠囊激發實驗 49 4.2.1螢光微膠囊於不同溫度之激發情形 49 4.2.2螢光隨溫度衰減情形 54 4.3實驗變因與重複性探討 65 4.3.1激發光源距離 65 4.3.2螢光溶液濃度 66 4.3.3螢光微膠囊之大小 68 4.3.4螢光微膠囊之內部溶液 69 4.3.5重複性實驗 71 4.4應用螢光強度量測溫度之分析 73 4.4.1螢光衰減量測 73 4.4.2單一螢光強度量測 75 4.4.3螢光比值量測 80 第五章 結論與建議 84 5.1結論 84 5.2建議 85 參考文獻 86 | |
dc.language.iso | zh-TW | |
dc.title | 以螢光微膠囊與低溫顯微鏡探討螢光影像之溫度效應 | zh_TW |
dc.title | Investigation of Temperature Effect on Fluorescent Imaging Using Fluorescent Microcapsules and Cryomicroscope | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳林祈,陳明汝 | |
dc.subject.keyword | 螢光溫度量測,螢光微膠囊,熱電致冷式低溫顯微鏡, | zh_TW |
dc.subject.keyword | Fluorescence Temperature Measurement,Fluorescent Microcapsules,Thermoelectric Cooler Cryomicroscope, | en |
dc.relation.page | 90 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-19 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物產業機電工程學研究所 | zh_TW |
顯示於系所單位: | 生物機電工程學系 |
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