請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47364
標題: | 以光譜影像技術檢測葉菜類蔬菜之硝酸鹽含量 Evaluation of Nitrate Content in Leafy Vegetable Using Spectral Imaging Techniques |
作者: | Jin-Shi Huang 黃君席 |
指導教授: | 陳世銘 |
關鍵字: | 光譜影像技術,近紅外光技術,硝酸鹽檢測, Spectral Imaging,Near-Infrared,Nitrate Measurement, |
出版年 : | 2011 |
學位: | 碩士 |
摘要: | 蔬菜為人類日常飲食中不可或缺之食物,然而蔬菜容易因天氣、施氮肥過度等因素,而殘留過多有害健康之硝酸鹽,因此本研究應用光譜影像檢測技術,建立出可快速檢測蔬菜中硝酸鹽濃度之方法。
本研究以皺葉萵苣為樣本,在種植過程中施加不同濃度之硝酸鹽溶液,以增加實驗樣本間硝酸鹽濃度之差異性,實驗進行時,樣本先以實驗型分光光度計NIRS 6500掃描及自行開發之高光譜影像系統對樣本進行取像,並將取得之影像經處理得樣本之吸收光譜資訊。最後將光譜資訊及實際硝酸鹽濃度進行MLR迴歸分析,結果顯示NIRS 6500搭配波長740, 496, 460, 680, 468 nm時之結果,rc可達0.66、SEC= 467 ppm,而驗證組之rv為0.57、SEV=470 ppm,顯示硝酸鹽與近紅外光光譜吸收度有一定程度之相關性。 以研究中自行開發的高光譜影像系統檢測硝酸鹽濃度,並搭配波長480, 690, 790, 930, 760 nm時,其結果rc更可高達0.91、SEC= 307 ppm,而驗證組之rv為0.59、SEV=589 ppm,此結果推測是高光譜影像系統具備空間解析能力,使吸收光譜與成份值間之關係更加吻合。研究最後將高光譜影像系統之檢測結果,與市售硝酸鹽檢測器進行比較,實驗過程中,市售之硝酸鹽檢測器在採樣時必須以蔬菜汁液進行量測,乃是破壞性之量測,相較於高光譜影像之非侵入、非破壞性,優劣其實已顯而易見,而實驗結果也證明高光譜影像系統的檢測能力明顯超越了市售硝酸鹽度計(SEC= 307 vs 534 ppm),高光譜影像系統同時也具備了其他技術所沒有的空間解析資訊,故本研究成果具有蔬菜產業之應用性。 Vegetable is an indispensable food. However, excessive nitrate in vegetable due to over-fertilization or weather is become a health problem to human. In this study, the spectral imaging technique is applied to establish a method, which can rapidly measure nitrate concentration in vegetables. Both NIRS 6500 spectrophotometer and hyperspectral imaging system (HIS) which was designed and developed in this study were used to measure the spectra and spectral images of loose-leaf lettuce. The lettuce was cultured under different nitrate concentrations. Multiple linear regression (MLR) was adopted to establish calibration models. The best model for NIRS 6500, was found at wavelengths 480, 602, 1,210, 1,580, 1,688 nm, and the results showed that rc= 0.66, SEC= 467 ppm, rv= 0.57, and SEV= 470 ppm. The best model for hyperspectral imaging system with wavelengths 480, 690, 790, 930, 760 nm indicated that rc= 0.91, SEC= 307 ppm, rv= 0.59, and SEV= 589 ppm. The result is much better than that of NIRS 6500 spectrophotometer, mainly because hyperspectral imaging system has spatial resolutions while spectrophotometer can only measure single spot. The hyperspectral imaging system was then compared with commercial nitrate meter, and HSIS still had better results (SEC= 307 vs 533 ppm). Since hyperspectral imaging system is a non-invasive non-destructive and rapid measurement method, it has great potential for applications to vegetable cultivation. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47364 |
全文授權: | 有償授權 |
顯示於系所單位: | 生物機電工程學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-100-1.pdf 目前未授權公開取用 | 2.5 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。