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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳進庭(Chin-Tin Chen) | |
dc.contributor.author | Pei-Shin Fang | en |
dc.contributor.author | 方佩馨 | zh_TW |
dc.date.accessioned | 2021-06-15T05:56:14Z | - |
dc.date.available | 2011-08-20 | |
dc.date.copyright | 2010-08-20 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-17 | |
dc.identifier.citation | 參考文獻
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47354 | - |
dc.description.abstract | 中文摘要
細胞骨架重組在細胞移動、細胞分裂、吞噬作用與形態發生的過程中扮演一相當重要的角色。在許多不同的細胞型態中,Rho family GTPases 的成員是主要調控細胞骨架,而Rnd1為Rho family GTPases 新的成員之一。五-氨基酮戊酸光動力效應 (ALA-PDT) 為一新興癌症治療方式,其作用為能夠選擇性地作用於粒線體產生單態氧與活性氧分子,因而導致細胞的死亡。在本研究中我們利用五-氨基酮戊酸光動力效應做為一可特定造成粒線體傷害的工具,並採用老鼠嗜鉻細胞瘤 (Rat Pheochromocytoma cells, PC12 cells) 做為類似神經細胞之模式細胞株,探討 Rnd1 在 ALA-PDT 抑制 PC12 細胞神經軸突延長的角色。 我們發現,利用Rnd1 siRNA 降低粒腺體功能缺失的 PC12 變異株細胞 Rnd1 表現,當存在有神經生長因子(nerve growth factor)的狀況下,細胞軸突外生現象減少並且可以正常延展。在其他相關訊息調控因子中,以ALA-PDT處理後,我們發現細胞內ROS與Rnd1 表現量增加,同時觀察到MAPKs與AMPK均被活化,抑制細胞內JNK, ERK 與 AMPK後Rnd1表現量減少,而抑制p38分子活性則對Rnd1無明顯影響。 | zh_TW |
dc.description.abstract | Abstract
Cytoskeletal rearrangements play a crucial role in cell motility, cytokinesis, phagocytosis and morphogenesis. Much progress has been made in elucidating the molecular mechanisms that control the organization of the actin cytoskeleton. It has been known that members of the Rho families are key regulators of the actin cytoskeleton in various cell types. Rnd1 is a new member of Rho families. 5- aminolevulinic acid mediated photodynamic therapy (ALA-PDT) can generate single oxygen and reactive oxygen species at mitochondria. In this study, ALA-PDT was used as a tool to damage the mitochondria in Rat Pheochromocytoma cells (PC12 cells). The purpose of this study is to address the role of Rnd1 in ALA-PDT- induced suppression of neurite elongation in PC12 cell. Here we show that, in the presence of NGF, downregulation of Rnd1 by Rnd1 siRNA suppressed neurite outgrowth and increase neurite elongation in PC12 variants. Furthermore, elevated content of ROS and Rnd1 was correlated with the activation of MAPKs and AMPK in ALA-PDT-treated PC12 cells. The upregulation of Rnd1 induced by ALA-PDT was suppressed by the inhibition of JNK, ERK and AMPK. These results indicate that the increased expression of Rnd1 might relate to the activation of ERK, JNK and AMPK, but not p38. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T05:56:14Z (GMT). No. of bitstreams: 1 ntu-99-R97b47411-1.pdf: 2547660 bytes, checksum: ca788819869e8a12c26db644149c4792 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 目錄
中文摘要………………….…………………………………………………….….…... i 英文摘要…………………………………………………………………………….… ii 目錄………………………………………………………………………………….… iii 圖目錄……………………………………………………………………………….… v 縮寫表………………….…………………………………………………………….…vi 第一章 緒論 1.1五胺基酮戊酸光動力療法…………………………………………..…... 1 1.2 活性氧分子……………………...…………………………………..…... 3 1.3 MAPKs .………………... …...…………………………………….…........7 1.4 AMPK…………….…………...…………………………………...…....... 9 1.5 Rho family.……………...……………………………………………...…10 1.6 Rnd1………………...………………………………………………….....10 1.7 Gsn………………...……………………………………………………....11 1.8老鼠嗜鉻細胞瘤 (Rat pheochromocytoma cells, PC12 cells)……..….....11 1.9 研究動機與目的………………...……………………………….............12 1.10 研究架構..........………………...……………………………….............14 第二章 材料與方法 2.1 藥品與儀器…………………………………………………………..… 15 2.2 細胞株……………………………………………………………….…. 17 2.3 細胞計數……………………………………………………….…….… 19 2.4 光動力治療………………………………………………………….…. 19 2.5 細胞存活率測定……………………………………………………..… 19 2.6反覆多次ALA- PDT 處理方法(ALA-PDT Selection)………….…20 2.7干擾RNA (RNA interference)……………………….………..……… 20 2.8 分化能力比較.………………………………………………………....21 2.9活性氧分子測定……………………………………………..………... 22 2.10 mRNA定量分析 ………….…………………………….…………....22 2.11 西方墨點法 (Western blot) ………………………..………….......….25 2 .12 統計方法………………………………………………......….......…..27 第三章 結果 3.1 Rnd1 在 ALA-PDT 抑制 PC12 細胞神經軸突延長的角色探討…..28 3.2 SH-SY5Y 變異株細胞Rnd1表現及分化能力……….……….………30 3.3 ROS影響 Rnd1 表現………………………………………….…......…32 3.4不同訊息分子 對於 ALA-PDT後 PC12 細胞Rnd1表現……..…...38 第四章 討論………………………………………………..……………………..…...41 第五章 結論………………………………………………………………..……….....46 附圖一~九……………………………………………………………………………..47 Figure 1- 21………………………………………………………………………….....56 參考文獻……………………………………………………………………………....88 附錄…………………………………………………………………………….............97 | |
dc.language.iso | zh-TW | |
dc.title | Rnd1 在 ALA-PDT 抑制 PC12 細胞神經軸突延長的角色探討 | zh_TW |
dc.title | The role of Rnd1 in ALA-PDT- induced suppression of neurite elongation in PC12 cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳儀莊,許瑞祥,黃慶璨 | |
dc.subject.keyword | PC12細胞,Rnd1,活性氧分子,五-氨基酮戊酸,光動力效應, | zh_TW |
dc.subject.keyword | C12 cells,Rnd1,ROS,5-Aminolevulinic acid,photodynamic treatment, | en |
dc.relation.page | 99 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-08-18 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
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