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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 李士傑(Shyh-Jye Lee) | |
dc.contributor.author | I-Chen Hung | en |
dc.contributor.author | 洪苡蓁 | zh_TW |
dc.date.accessioned | 2021-06-15T05:53:38Z | - |
dc.date.available | 2015-08-19 | |
dc.date.copyright | 2010-08-19 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-18 | |
dc.identifier.citation | Aman, A., Piotrowski, T., 2008. Wnt/beta-catenin and Fgf signaling control collective cell migration by restricting chemokine receptor expression. Dev Cell 15, 749-761.
Baker, C.V., Bronner-Fraser, M., 2001. Vertebrate cranial placodes I. Embryonic induction. Dev Biol 232, 1-61. Blaser, H., Reichman-Fried, M., Castanon, I., Dumstrei, K., Marlow, F.L., Kawakami, K., Solnica-Krezel, L., Heisenberg, C.P., Raz, E., 2006. Migration of zebrafish primordial germ cells: a role for myosin contraction and cytoplasmic flow. Dev Cell 11, 613-627. Brosamle, C., Halpern, M.E., 2002. Characterization of myelination in the developing zebrafish. Glia 39, 47-57. Cruz, S., Shiao, J.C., Liao, B.K., Huang, C.J., Hwang, P.P., 2009. Plasma membrane calcium ATPase required for semicircular canal formation and otolith growth in the zebrafish inner ear. J Exp Biol 212, 639-647. Dambly-Chaudiere, C., Cubedo, N., Ghysen, A., 2007. Control of cell migration in the development of the posterior lateral line: antagonistic interactions between the chemokine receptors CXCR4 and CXCR7/RDC1. BMC Dev Biol 7, 23. Dambly-Chaudiere, C., Sapede, D., Soubiran, F., Decorde, K., Gompel, N., Ghysen, A., 2003. The lateral line of zebrafish: a model system for the analysis of morphogenesis and neural development in vertebrates. Biol Cell 95, 579-587. David, N.B., Sapede, D., Saint-Etienne, L., Thisse, C., Thisse, B., Dambly-Chaudiere, C., Rosa, F.M., Ghysen, A., 2002. Molecular basis of cell migration in the fish lateral line: role of the chemokine receptor CXCR4 and of its ligand, SDF1. Proc Natl Acad Sci U S A 99, 16297-16302. Denzel, A., Molinari, M., Trigueros, C., Martin, J.E., Velmurgan, S., Brown, S., Stamp, G., Owen, M.J., 2002. Early postnatal death and motor disorders in mice congenitally deficient in calnexin expression. Mol Cell Biol 22, 7398-7404. Ellgaard, L., Riek, R., Braun, D., Herrmann, T., Helenius, A. and Wuthrich, K. (2001). Three-dimensional structure topology of the calreticulin P-domain based on NMR assignment. FEBS Lett 488, 69-73. Garner, J.N., Joshi, B., Jagus, R., 2003. Characterization of rainbow trout and zebrafish eukaryotic initiation factor 2alpha and its response to endoplasmic reticulum stress and IPNV infection. Dev Comp Immunol 27, 217-231. Germana, A., Paruta, S., Germana, G.P., Ochoa-Erena, F.J., Montalbano, G., Cobo, J., Vega, J.A., 2007. Differential distribution of S100 protein and calretinin in mechanosensory and chemosensory cells of adult zebrafish (Danio rerio). Brain Res 1162, 48-55. Gilmour, D., Knaut, H., Maischein, H.M., Nusslein-Volhard, C., 2004. Towing of sensory axons by their migrating target cells in vivo. Nat Neurosci 7, 491-492. Gold, L.I., Eggleton, P., Sweetwyne, M.T., Van Duyn, L.B., Greives, M.R., Naylor, S.M., Michalak, M., Murphy-Ullrich, J.E., 2010. Calreticulin: non-endoplasmic reticulum functions in physiology and disease. FASEB J 24, 665-683. Harris, J.A., Cheng, A.G., Cunningham, L.L., MacDonald, G., Raible, D.W., Rubel, E.W., 2003. Neomycin-induced hair cell death and rapid regeneration in the lateral line of zebrafish (Danio rerio). J Assoc Res Otolaryngol 4, 219-234. Harris, M.R., Yu, Y.Y., Kindle, C.S., Hansen, T.H., Solheim, J.C., 1998. Calreticulin and calnexin interact with different protein and glycan determinants during the assembly of MHC class I. J Immunol 160, 5404-5409. Hava, D., Forster, U., Matsuda, M., Cui, S., Link, B.A., Eichhorst, J., Wiesner, B., Chitnis, A., Abdelilah-Seyfried, S., 2009. Apical membrane maturation and cellular rosette formation during morphogenesis of the zebrafish lateral line. J Cell Sci 122, 687-695. Kerstetter, A.E., Azodi, E., Marrs, J.A., Liu, Q., 2004. Cadherin-2 function in the cranial ganglia and lateral line system of developing zebrafish. Dev Dyn 230, 137-143. Lecaudey, V., Cakan-Akdogan, G., Norton, W.H., Gilmour, D., 2008. Dynamic Fgf signaling couples morphogenesis and migration in the zebrafish lateral line primordium. Development 135, 2695-2705. Ledent, V., 2002. Postembryonic development of the posterior lateral line in zebrafish. Development 129, 597-604. Li, J., Puceat, M., Perez-Terzic, C., Mery, A., Nakamura, K., Michalak, M., Krause, K.H., Jaconi, M.E., 2002. Calreticulin reveals a critical Ca(2+) checkpoint in cardiac myofibrillogenesis. J Cell Biol 158, 103-113. Li, Q., Shirabe, K., Kuwada, J.Y., 2004. Chemokine signaling regulates sensory cell migration in zebrafish. Dev Biol 269, 123-136. Ma, E.Y., Raible, D.W., 2009. Signaling pathways regulating zebrafish lateral line development. Curr Biol 19, R381-386. Metcalfe, W.K., Kimmel, C.B., Schabtach, E., 1985. Anatomy of the posterior lateral line system in young larvae of the zebrafish. J Comp Neurol 233, 377-389. Michalak, M., Milner, R.E., Burns, K., Opas, M., 1992. Calreticulin. Biochem J 285 ( Pt 3), 681-692. Montgomery, J., Carton, G., Voigt, R., Baker, C., Diebel, C., 2000. Sensory processing of water currents by fishes. Philos Trans R Soc Lond B Biol Sci 355, 1325-1327. Nechiporuk, A., Raible, D.W., 2008. FGF-dependent mechanosensory organ patterning in zebrafish. Science 320, 1774-1777. Nicolson, T., 2005. The genetics of hearing and balance in zebrafish. Annu Rev Genet 39, 9-22. Nixon, S.J., Carter, A., Wegner, J., Ferguson, C., Floetenmeyer, M., Riches, J., Key, B., Westerfield, M., Parton, R.G., 2007. Caveolin-1 is required for lateral line neuromast and notochord development. J Cell Sci 120, 2151-2161. Olkku, A., Mahonen, A., 2009. Calreticulin mediated glucocorticoid receptor export is involved in beta-catenin translocation and Wnt signalling inhibition in human osteoblastic cells. Bone 44, 555-565. Paquet, M.E., Leach, M.R., Williams, D.B., 2005. In vitro and in vivo assays to assess the functions of calnexin and calreticulin in ER protein folding and quality control. Methods 35, 338-347. Park, M., Moon, R.T., 2002. The planar cell-polarity gene stbm regulates cell behaviour and cell fate in vertebrate embryos. Nat Cell Biol 4, 20-25. Raible, D.W., Kruse, G.J., 2000. Organization of the lateral line system in embryonic zebrafish. J Comp Neurol 421, 189-198. Robu, M.E., Larson, J.D., Nasevicius, A., Beiraghi, S., Brenner, C., Farber, S.A., Ekker, S.C., 2007. p53 activation by knockdown technologies. PLoS Genet 3, e78. Rosenbaum, E.E., Hardie, R.C., Colley, N.J., 2006. Calnexin is essential for rhodopsin maturation, Ca2+ regulation, and photoreceptor cell survival. Neuron 49, 229-241. Santos, F., MacDonald, G., Rubel, E.W., Raible, D.W., 2006. Lateral line hair cell maturation is a determinant of aminoglycoside susceptibility in zebrafish (Danio rerio). Hear Res 213, 25-33. Valentin, G., Haas, P., Gilmour, D., 2007. The chemokine SDF1a coordinates tissue migration through the spatially restricted activation of Cxcr7 and Cxcr4b. Curr Biol 17, 1026-1031. Villablanca, E.J., Renucci, A., Sapede, D., Lec, V., Soubiran, F., Sandoval, P.C., Dambly-Chaudiere, C., Ghysen, A., Allende, M.L., 2006. Control of cell migration in the zebrafish lateral line: implication of the gene 'tumour-associated calcium signal transducer,' tacstd. Dev Dyn 235, 1578-1588. Wada, I., Ou, W.J., Liu, M.C., Scheele, G., 1994. Chaperone function of calnexin for the folding intermediate of gp80, the major secretory protein in MDCK cells. Regulation by redox state and ATP. J Biol Chem 269, 7464-7472. Walther, R.F., Lamprecht, C., Ridsdale, A., Groulx, I., Lee, S., Lefebvre, Y.A., Hache, R.J., 2003. Nuclear export of the glucocorticoid receptor is accelerated by cell fusion-dependent release of calreticulin. J Biol Chem 278, 37858-37864. Williams, J.A., Holder, N., 2000. Cell turnover in neuromasts of zebrafish larvae. Hear Res 143, 171-181. Yanicostas, C., Ernest, S., Dayraud, C., Petit, C., Soussi-Yanicostas, N., 2008. Essential requirement for zebrafish anosmin-1a in the migration of the posterior lateral line primordium. Dev Biol 320, 469-479. Zhang, X., Szabo, E., Michalak, M., Opas, M., 2007. Endoplasmic reticulum stress during the embryonic development of the central nervous system in the mouse. Int J Dev Neurosci 25, 455-463. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47287 | - |
dc.description.abstract | 側線是兩生類與魚類特有的器官,用以感知外界水流的方向與速率.此器官是由側線神經母細胞所組成,其中包含了纖毛細胞與支持細胞。為了研究可能參與在側線發育的候選基因,我們利用斑馬魚資料庫網站(ZFIN)搜尋在側線神經母細胞所表現的基因,並發現有一群鈣離子結合蛋白特定表現在這個位置。鈣離子是一個熟知的二級傳訊者,並參與許多細胞活動包括細胞黏合與移動。其中我們對calreticulin(鈣網蛋白) 和 calnexin(鈣聯蛋白) 特別感興趣. 鈣網蛋白和鈣聯蛋白為內質網中伴隨蛋白(chaperon)之一,其主要功能在於幫助新生成醣蛋白的疊合與品質管制。加上其可與鈣離子結合之特性,鈣聯蛋白可影響多種細胞活性如內質網壓力反應,細胞凋亡,細胞黏著及胞器生成等。而此等細胞活性為動物早期胚胎發育所必需,因此我們認為鈣網蛋白和鈣聯蛋白可能在側線發育過程扮演一重要之角色,並選用模式生物斑馬魚研究其功能。斑馬魚的鈣網蛋白和鈣聯蛋白基因與人類的基因具有高度相似度,藉由全固定原位雜合我們發現兩基因的mRNA廣泛表現於早期斑馬魚胚中,而在受精後36小時開始表現在側線神經母細胞初始細胞中,爾後更專一地表現在側線神經母細胞。藉由顯微注射反義吗啉寡聚核苷酸(morpholino oligonucleotides, MO)降低鈣網蛋白和鈣聯蛋白在斑馬魚胚之表現量,我們發現隨著鈣聯蛋白MO劑量的提高,利用各種染劑檢査側線神經母細胞叢、每單位內的神經母細胞細胞及纖毛,我們發現其數量均有逐漸減少的情形,而這些缺陷可以藉由同時注入體外合成的鈣聯蛋白mRNA 所部分回復。但這樣的情形在減少鈣網蛋白表現量減弱的斑馬魚中並沒有明顯的效果。再者以原位雜合觀察纖毛細胞的記號基因eya1與支持細胞的記號基因claudinb亦顯示兩者皆因鈣聯蛋白之弱化而降低,此等結果顯示鈣聯蛋白可能參與側線纖毛細胞與支持細胞形成的過程。而SDF1a/CXCR7已知為引導神經母細胞細胞初始細胞移動的訊息,然 SDF1a mRNA之表現卻因鈣聯蛋白之弱化而片段化,其顯示鈣聯蛋白可能經由影響SDF1a/CXCR7訊息來調節斑馬魚側線細胞之形成及發育過程。 | zh_TW |
dc.description.abstract | Lateral line is a mechanosensory system in fish and amphibian to detect local water flow and pressure. This system comprises a series of neuromast clusters, which are formed by sensory hair cells and support cells. To further investigate candidate genes which may be involved in lateral line development, we screened genes which are expressed in the lateral line of zebrafish by examining available expression patterns in the Zebrafish Information Network (ZFIN, http://zfin.org). We found a group of calcium binding proteins expressed specifically at the lateral line system. Calcium is a well known second messenger that exerts many cellular activities, including cell adhesion and migration, via its binding proteins. At these candidate genes we are particularly interested in calreticulin and calnexin. These genes are an endoplasmic reticulum (ER) calcium binding protein and known chaperon to ensure proper folding and quality control of newly synthesized glycoproteins that allow it to participate in cellular activities like ER stress, apoptosis, cell adhesion and organogenesis. Those cellular activities are essential for embryogenesis. Therefore, we hypothesized that calnexin may play a role during lateral line development. We have identified zebrafish calreticulin and calnexin with high similarity to their human homologs. Whole-mount in situ hybridization (WISH) and RT-PCR analyses revealed that calreticulin and calnexin mRNAs are ubiquitously expressed during early embryogenesis in zebrafish. WISH analysis further showed that the expression domain of calreticulin and calnexin was first observed at the migrating primordium of lateral line at 36 h post fertilization and the calreticulin and calnexin mRNAs were specifically localized to the neuromasts. Knockdown of calnexin by antisense morphonino oligonucleotides (MOs) resulted in a dose-dependent reduction in numbers of neuromast clusters, neuromasts per cluster and hair cells in lateral line that could be rescued by over-expressing respective mRNAs. WISH analysis by neuromast maker eya1and neuromast support cell marker claudinb, both showed reduced cluster numbers in calnexin morphants. It indicated that calnexin may be involved in both neuromast and support cell deposition. But these phenotypes were not obvious in the calreticulin morphants. Furthermore, the abolition of calnexin expression resulted in fragmented SDF1a expression, which implies that loss of calnexin might interfere with the SDF/CXCR7 cue for neuromast primodium migration. These results suggest that calnexin is essential for neuromast formation during lateral line development. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T05:53:38Z (GMT). No. of bitstreams: 1 ntu-99-R97b41007-1.pdf: 2014928 bytes, checksum: 33ccdb29b8c38a4b237bcda67011d331 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 中文摘要 I
Abstract III Contents V List of Figures VI Introduction 1 Materials and Methods 5 Fish breeding and embryo collection 5 Total RNA isolation and RT-PCR analysis 5 Antisense morpholinos and constructs for ectopic gene expression 6 Sequence analysis of calnexin 8 Calnexin translation blocking MO efficiency check 9 Microinjection 9 Lateral line hair cells staining 10 Whole mount in situ hybridization 10 Statistics 12 Results 13 In silico cloning and gene analysis 13 Expression of calreticulin and calnexin in zebrafish lateral line system 15 Knockdown of calnexin causes posterior lateral line developmental defect 17 p53 MO partially rescued the survival rate of the calnexin morphant but could not restore the neuromast defect 19 Knockdowm of calnexin affect the migration and deposition of posterior lateral line primodium 20 Depletion of calnexin disrupted sdf1a/cxcr7b signaling 22 Discussion 23 The expression of calnexin and calreticulin during development 23 Functional characterization of calreticulin and calnexin 24 Calnexin is required for posterior lateral line development 25 Knockdown calnexin affect the cxcr7b/sdf1a signaling 28 References 32 Figures & Legends 39 Tables 61 | |
dc.language.iso | en | |
dc.title | 鈣聯蛋白(calnexin)弱化抑制斑馬魚側線發育 | zh_TW |
dc.title | Knockdown of calnexin inhibits lateral line development in zebrafish | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蕭崇德(Chung-Der Hsiao),顏裕庭(Yu-Ting Yan),許文明(Wen-Ming Hsu) | |
dc.subject.keyword | 側線,鈣聯蛋白,鈣網蛋白,側線神經母細胞, | zh_TW |
dc.subject.keyword | lateral line,neuromast,calreticulin,calnexin,promodium,deposition, | en |
dc.relation.page | 63 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-08-18 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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