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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林讚標 | |
dc.contributor.author | Wei-Wen Kuo | en |
dc.contributor.author | 郭瑋文 | zh_TW |
dc.date.accessioned | 2021-06-15T05:51:33Z | - |
dc.date.available | 2012-08-19 | |
dc.date.copyright | 2010-08-19 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-17 | |
dc.identifier.citation | REFERENCES
Abe, H., Urao, T., Ito, T., Seki, M., Shinizaki., and Yamaguchi-Shinozaki, K. (2003). Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell 15: 63-78. Allen, M.D., Yamasaki, K., Ohem-Takagi, M., Tateno, M., and Suzuki, M. (1998). A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA. EMBO J. 17: 5484-5496. Banno, H., Ikeda, Y., Niu, Q.W., Chua, N.H. (2001). Overexpression of Arabidopsis ESR1 induces initiation of shoot regeneration. Plant Cell 13: 2609 –2618. Berrocal, M., and Molina, A. (2004). Ethylene Response Factor 1 Mediates Arabidopsis Resistance to the Soilborne Fungus Fusarium oxysporum. MPMI 17: 763-770. Broun, P., Poindexter, P., Osborne, E., Jiang, C.Z., Riechmann, J.L. (2004). WIN1, a transcriptional activator of epidermal wax accumulation in Arabidopsis. Proc. Natl. Acad. Sci. USA 101: 4706–4711. Cokol, M., Nair, R., and Rost, B. (2000). Finding nuclear localization signals. EMBO Rep. 1: 411-415. Deblaere, R., Reynaerts, A., Hofte, H., Hernalsteens, J.P., Leemans, J., and Van Montagu, M. (1987). Vectors for cloning in plant cells. Meth. Enzymol. 153: 277-292. Doyle, J.J., and Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. Focus 12: 13-15. Dubouzet, J.G., Sakuma, Y., Ito, Y., Kasuga, M., and Dubouzet, E.G. (2003). OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt and cold-responsive gene expression. Plant J. 33: 751-763. Earley, K.W., Hang, J.R., Pontes, O., Opper, K., Juehne, T., Song, K., and Pikaard, C.S. (2006). Gateway-compatible vectors for plant functional genomics and proteomics. Plant J. 45: 616-629. Elliott, R.C., Betzner, A.S., Huttner, E., Oakes, M.P., Tucker, W.Q., Gerentes, D., Perez, P., and Smyth, D.R. (1996). AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth. Plant Cell 8: 155-168. Fujimoto, S.Y., Ohta, M., Usui, A., Shinshi, H., and Ohne-Takagi, M. (2000). Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression. Plant Cell 12: 393-404. Fujita, M.F.Y., Maruyama, K., Seki, M., Hiratsu, K., Ohme-Takagi, M., Tran, L.S., Tamaguchi-Shinozaki, K., and Shinozaki, K. (2004). A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway. Plant J. 39: 863-876. Gilmour, S.J., and Thomashow, M.F. (1991). Cold acclimation and cold-regulated gene expression in ABA mutants of Arabidopsis thaliana. Plant Mol. Biol. 17: 1233-1240. Gosti, F., Beaudoin, N., Serizet, C., Webb, A.A., and Vartanian, N. (1999). ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling. Plant Cell 11: 1897-1910. Hao, D., Ohme-Takagi, M., and Sarai, A. (1998). Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant. J. Biol. Chem. 273: 26857-26861. Higo, K., Ugawa, Y., Iwamoto, M., and Korenaga, T. (1999). Plant cis-acting regulatory DNA elements (PLACE) database: 1999. Nucleic Acids Res. 27: 297-300. Hoekema, A., Hirsch, P.R., Hooykaas, P.J.J., and Schilperoort, R.A. (1983). Binary vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303: 179-180. Jaglo-Ottosen, K.R., Gilmour, S. J., Zarka, D.G., Schabenberger, O., and THomashow, M.F. (1998). Arabidopsis CBF1 overexpression induceds cor genes and enhances freezing tolerance. Science 280: 104-106. Jofuku, K.D., Den Boer, B.G., Van Montagu, M., and Okamuro, J.K. (1994). Control of Arabidopsis flower and seed development by the homeotic gene APETALA2. Plant Cell 6: 1211-1225. Kasuga, M., Liu, Q., Miura, S., Yamaguchi-Shinozaki, K., and Shinozaki, K. (1999). Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nat. Biotechnol. 17: 287-291. Kasuga, M., Seki, M., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2004). A combination of the Arabidopsis DREB1A gene and stress-inducible rd29A promoter improved drought- and low-temperature stress tolerance in tobacco by gene transfer. Plant Physiol. 45: 346-350. Kizis, D.L.V. and Pages, M. (2001). Role of AP2/REBP transcription factors in gene regulation during abiotic stress. FEBS. Letters 498: 187-189. Knight, H., Zarka, D.G., Okamoto, H., Thomashow, M.F., and Knight, M.R. (2004). Abscisic acid induces CBF gene transcription and subsequent induction of cold-regulated genes via the CRT promoter element. Plant Physiol. 135: 1710-1717. Koroleva, O.A., Tom;inson, M.L., Leader, D., Shaw, P., and Doonan, J.H. (2005). High-throughput protein localization in Arabidopsis using Agrobacterium-mediated transient expression of GFP-ORF fusions. Plant J. 41: 162-174. Krizek, B.A., Prost, V., and Macias, A. (2000). AINTEGUMENTA promotes petal identity and acts as a negative regulator of AGAMOUS. Plant Cell 12: 1357-1366. Lescot, M., Dehais, P., Thijs, G., Marchal, K., Moreau, Y., Peer, Y.V., Rouze, P., and Rombauts, S. (2002). PlantCare, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequence. Oxford University Press 30: 325-327. Lorenzo, O., Piqueras, R., Sanchez-Serrano, J.J., Solano, R. (2003). ETHYLENE RESPONSE FACTOR1 integrates signals from ethylene and jasmonate pathways in plant defense. Plant Cell 15: 165-178. Liu, Q.K.M., Sakuma, Y., Abe, H., Miura, S., Yamaguchi-Shinozaki, K., and Shinozaki, K. (1998). Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10: 1391-1406. Nakkano, T., Shinozaki, K., Fujimura, T., and Shinshi, H. (2006). Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol. 140: 411-432. Nole-Wilson, S., Tranby, T.L., and Krizek, B.A. (2005). AINTEGUMENTA-like (AIL) genes are expressed in young tissues and may specify meristematic or division-competent states. Plant Mol. Biol. 57: 613-628. Nordin, K., Heino, P., and Palva, E.T. (1991). Separate signal pathways regulate the expression of a low-temperature-induced gene in Arabidopsis thaliana (L.) Heynh. Plant Mol. Biol. 16: 1061-1074. Ohme-Takagi, M., and Shinshi, H. (1995). Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. Plant Cell 7: 173-182. Ohta, M., Matsui, K., Hiratsu, K., Shinshi, H., and Ohme-Takagi, M. (2001). Repression Domains of Class II ERF Transcriptional Repressors Share an Essential Motif for Active Repression. Plant Cell 13: 1959-1968. Qu, L.-J., and Zhu, Y.-X. (2006). Transcription factor families in Arabidopsis: major progress and outstanding issues for future research. Curr. Opin. Plant Biol. 9: 544-549. Reddy, A.R., Chaitanya, K.V., and Vivekanandan, M. (2004). Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J. Plant Phusiol. 161: 1189-1202. Riechmann, J.L., and Meyerowitz, E.M. (1998). The AP2/EREBP family of plant transcription facors. Biol. Chem. 379: 633-646. Riechmann, J.L., and Ratcliffe, O.J. (2000). A genomic perspective on plant transcription factors. Curr. Opin. Plant Biol. 3: 423-434. Rozema, J., and Flowers, T. (2008). Crop for a Salinized World. Science 322: 1478-1480. Sakuma, Y., Maruyama, K., Qin, F., Osakabe, Y., Shinozaki, K., and Yamaguchi-Shinizali, K. (2006a). Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression. Plant Cell 18: 1292-1309. Sakuma, Y., Maruyama, K., Qin, F., Osakabe, Y., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2006b). Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression. Proc. Natl. Acad. Sci. USA 103: 18822-18827. Shinozaki, K., and Yamaguchi-Shinozaki, K. (1997). Gene Expression and Signal Transduction in Water-Stress Response. Plant Physiol. 115: 327-334. Shinozaki. K., and Yamaguchi-Shinozaki, K. (2007). Gene networks involved in drought stress response and tolerance. J. Exp. Bot. 58: 221-227. Solano, R., Stepanovsa, A., Qimin, C., and Ecker, J.R. (1998). Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1. Gene & Dev. 12: 3703-3714. Stockinger, E.J., Gilmour, S.J., and Thomashow, M.F. (1997). Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-reoeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc. Natl. Acad. Sci. USA 94: 1035-0140. Sun, S., Yu, J,-P., Chen, F., Zhao, T.-J., Fang, X.-H., Li, Y.-Q., and Sui, S.-F. (2008). TINY, a Dehydration-responsive Element (DRE)-binding Protein-like Transcription Factor Connecting the DRE- and Ethylene-responsive Element-mediated Signaling Pathways in Arabidopsis. J. Biol. Chem. 283: 6261-6271. Uno, Y.F.T., Abe, H., Yoshida, R., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2000). Arabidopsis basic leucine zipper transcriptional transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proc. Natl. Acad. Sci. USA 97: 11632-11637. van der Graaff, E., Dulk-Ras, AD., Hooykaas, P.J., Keller, B. (2000). Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana. Dev. 127: 4971–4980. van der Fits, L., and memelink, J. (2001). The jasmonate-inducible AP2/ERF-domain transcription factor ORCA3 activates gene expression via interaction with a jasmonate-responsive promoter element. Plant J. 25: 43-53. Vorosmarty, C.J., Green, P., Salisbury, J., and Lammers, R.B. (2000). Global water resources: vulnerability from climate change and population growth. Science 289: 284-288. Weiste, C., Iven, T., Fischer, U., Onate-Sanchez, L., and Droge-Laser, W. (2007). In planta ORFeome analysis by large-scale over-expression of GATEWAY-compatible cDNA clones: screening of ERF transcription factors involved in abiotic stress defense. Plant J. 52: 382-390. Wu, L., Zhang, Z., Zhang, H., Wang, X.C., and Huang, R. (2008). Transcriptional Modulation of Ethylene Response Facto Protein JERF3 in the Oxidative Stress Response Enhances Tolerance of Tobacco Seedlings to Salt, Drought, and Freezing. Plant Physiol. 148: 1953-1963. Yamaguchi-Shinozaki, K., and Shinozaki, K. (1994). A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell 6: 251-264. Yamaguchi-Shinazaki, K., and Shinozaki,K. (2005). Oranization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters. Trends Plant Sci. 10: 88-94. Yamamoto, S., Suzaki, K., and Shinshi, H. (1999). Elicitor-responsive, ethylene-independent activation of GCC box-mediated transcription that is regulated by both protein phosphorylation and dephosphorylation in cultured tobacco cells. Plant J. 20: 571-579. Yang, Z., Tian, L., Latoszak-Green, M., Brown, D., and Wu, K. (2005). Arabidopsis ERF4 is a transcriptional repressor capable of modulating ethylene and abscisic acid responses. Plant Mol. Biol. 25: 585-596. Zhang, J.Y., Broeckling, C.D., Blancaflor, E.B., Sledge, M.K., Sumner, L.W., Wang, Z.Y. (2005). Overexpression of WXP1, a putative Medicago truncatula AP2domain-containing transcription factor gene, increases cuticular wax accumulation and enhances drought tolerance in transgenic alfalfa (Medicago sativa). Plant J. 42: 689–707. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47228 | - |
dc.description.abstract | 環境中的許多不利因子皆會影響植物的生長,其中非生物性逆境諸如乾旱、高鹽、高低溫等。以乾旱逆境為例,在水分缺乏的情形下會干擾植物的正常生長,然而植物會利用各種生理與生化機制來抵抗乾旱而達到生存的目的。前人研究顯示植物會於轉錄層面進行基因的調控,促使下游基因使之活化或抑制以因應環境逆境。我們在2008年9月從Dr. Wolfgang Droge-Laser 實驗室獲得ERF轉錄因子的大量表現之阿拉伯芥種子。而在前人的研究已知一些ERF轉錄因子會參予在非生物性逆境的調控上,然而還有許多成員都還不清楚其功能。所以我們篩選具有抗旱的植株並收其後代種子而進行這些轉錄因子之功能研究。經由定序結果比對後得到2個阿拉伯芥的基因,Ethylene Response Factor 1 (ERF1)及 Aintegumenta-like 7 (AIL7)。此轉錄因子皆屬於AP2/ERF superfamily的成員,分別含有一個及二個高度保留性的AP2 DNA binding domain。根據eFP的資料庫數據得知,ERF1會受高鹽所誘導,尤其在根部的位置;而AIL7則會受乾旱、高鹽及滲透壓所誘導,但在高低溫逆境下則無誘導表現。此外,ERF1及AIL7皆不受ABA誘導表現,故可能參予在ABA-independent pathway。首先,我們測試了在高鹽底下的種子發芽率,發現二個大量表現轉殖品系發芽率都較野生型品系高出許多;接著觀察在高鹽環境下的根系發展情形,結果顯示出野生型阿拉伯芥的根長都較二個突變株來的短;而進一步直接使用鹽水澆灌觀察其存活率,有趣的是我們看到了在ERF1的大量表現轉殖株有耐鹽的情形;然而在AIL7片段基因大量表現的轉殖株卻有相反的結果,也就是對鹽逆境較為敏感。最後我們處理缺水逆境也看到了ERF1轉殖株有較高的存活率;反之,AIL7轉殖株的存活植株相較野生型植株來的低許多。而我們構築了ERF1及AIL7與GFP的結合蛋白,透過原生質體表現後,在CaMV35S啟動子調控下,可看到綠色螢光訊號位於細胞核中。在loss-of-function研究中,T-DNA insertion knockout突變株與野生型植株間並沒有看到有明顯的差異性。因為篩選到的AIL7大量表現轉植株只有插入部分的片段,所以我們利用了轉錄活性分析探討其功能。短暫表現轉錄活性分析結果顯示全長(1/1497)及C端片段(688/1497)的AIL7並無轉錄活性;而N端部分片段(175/687)卻有顯著的轉錄活性。根據以上實驗結果我們首度發現在非生物性逆境下ERF1扮演著正向調控角色;而部分片段的AIL7是具有轉錄上的活性,並且在非生物性逆境下扮演著負調控的角色。 | zh_TW |
dc.description.abstract | Various abiotic stresses including drought, high salinity, and extreme temperatures affect plant growth. To survive, plants have to react and adapt to these stresses. Exposure to these unfavorable conditions leads to various physiological and biochemical alternations in the process of acquiring stress tolerance. Regulation of genes at transcriptional level has been described in response to environmental stresses. Particularly, many transcription factor (TF) genes are stress inducible, and function in regulating stress signal transduction pathways. Here we screened ERF TF overexpressed transgenic seeds by drought stress, and two lines, containing Ethylene Response Factor 1 (ERF1) and Aintegumenta-Like 7 (AIL7), showed transgene respectively phenotype from the WT plants. These two genes have highly conserved AP2 DNA binding domain and are induced by salt and/or osmotic stresses but not by abscisic acid (ABA), implicated they are involved in the ABA-independent pathway. We have found that the germination rate and root elongation in these transgenics are greater than those of wild type. In salt and drought stress experiments, ERF1 overexpression (OE) transgenic plants were more tolerant to salt and drought stresses, but AIL7 partial fragment OE transgenic plants were more sensitive. Localization assay of ERF1-GFP and AIL7-GFP showed ERF1 and AIL7 were located in the nucleus. Loss-of-function study of T-DNA knockout mutants did not show significant differences from wild-type plant. Functional domain study of AIL7 by the transient expression assay exhibited that full length (1/1497) and C-terminal (688/1497) of AIL7 had no transcriptional activity, while N-terminal partial fragment (175/687) showed distinct transcriptional activity. These results suggested that ERF1 may play a positive role to enhance tolerance in abiotic stress, while partial fragment of AIL7 have transcriptional activity and act as a repressor in abiotic stress. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T05:51:33Z (GMT). No. of bitstreams: 1 ntu-99-R97b42005-1.pdf: 34007886 bytes, checksum: 0e241fb6b384e7b879ba16eadbcba91e (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 目錄
口試委員審定書 致謝 中文摘要………………………………………………………………………………1 ABSTRACT…………………………………………………………………………..2 縮寫對照表……………………………...…………………………………………….3 第一章 序論…………………………………………………………………………..4 1.1 乾旱逆境的訊息傳導………………………………………………………….4 1.2 GCC box和DRE/CRT element………………………………………………..4 1.3 在阿拉伯芥中的AP2/ERF轉錄因子………………………………………...5 1.4 AP2/ERF轉錄因子在逆境反應調控上的功能……………………………….6 1.5 ERF1及AIL7的背景………………………………………………………….6 1.6 實驗策略與目標……………………………………………………………….7 第二章 材料與方法…………………………………………………………………..8 2.1植物材料、生長條件及轉殖株的篩選............................................................8 2.2 ERF1及AIL7基因序列分析…………………………………………………..8 2.3 DNA萃取、PCR及基因定序…………………………………………………8 2.4 RNA萃取及cDNA合成之RT-PCR…………………………………………..9 2.5南方轉漬分析 (Southern Blot)………………………………………………...9 2.6鹽逆境下的發芽與根部延長試驗……………………………………………..9 2.7突變株的非生物性逆境容忍分析……………………………………………..9 2.8構築及短暫表現的融合蛋白之細胞位置……………………………………..9 2.9植物轉形構築及轉基因植物的建立…………………………………………10 2.10報導質體(Reporter)與作用質體(Effector)之構築………………………...10 2.11短暫表現之轉錄活性分析…………………………………………………..11 第三章 結果 3.1 ERF轉錄因子家族的大量表現轉殖種子之篩選……………………………12 3.2阿拉伯芥裡的ERF1及AIL7基因……………………………………………12 3.3在非生物性逆境與荷爾蒙處理下ERF1及AIL7的表現情形………………12 3.4 ERF1和AIL7片段基因大量表現轉殖株之T-DNA插入數目及mRNA表現程度……………………………………………………………………………12 3.5 ERF1promoter::GUS轉基因植物…………………………………………….13 3.6 ERF1與AIL7片段基因大量表現轉殖株之鹽逆境下種子發芽…………….13 3.7 ERF1與AIL7片段基因大量表現轉殖株之鹽逆境下的根長度…………….13 3.8 ERF1大量表現轉殖株在高鹽和乾旱逆境下有較高的耐受性……………..13 3.9 AIL7片段基因大量表現轉殖株在高鹽和乾旱逆境下有較低的耐受性…..14 3.10 ERF1及AIL7蛋白質在細胞內的位置……………………………………..14 3.11 erf1及ail7突變株在非生物性逆境耐受分析………………………….......14 3.12 AIL7的轉錄活性分析………………………………………………………15 第四章 討論 4.1 ERF1及AIL7受到高鹽或乾旱逆境誘導表現……………………………..16 4.2 ERF1及AIL7表現位置位於細胞核……………………………………….16 4.3 ERF1及AIL7在種子及幼苗階段對鹽逆境較為不敏感………………….16 4.4 ERF1及AIL7在非生物性逆境上的耐受能力…………………………….17 4.5 erf1及ail7突變株在非生物性逆境下並無顯著差異……………………..17 4.6 AIL7的N端部分之功能探討……………………………………………..18 第五章 圖表………………………………………………………………………..20 參考文獻……………………………………………………………………………36 附錄…………………………………………………………………………………41 | |
dc.language.iso | zh-TW | |
dc.title | AtERF1及AIL7在非生物性逆境耐受性上分別扮演的角色 | zh_TW |
dc.title | Roles of AtERF1 and AIL7 in Abiotic Stress Tolerance | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 謝明勳,陳仁治,鄭石通 | |
dc.subject.keyword | 阿拉伯芥,非生物性逆境,耐受性, | zh_TW |
dc.subject.keyword | Arabidopsis,Abiotic stress,Tolerance, | en |
dc.relation.page | 62 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-08-18 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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