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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張惠婷 | |
| dc.contributor.author | Yuan-Lin Ferng | en |
| dc.contributor.author | 馮苑琳 | zh_TW |
| dc.date.accessioned | 2021-06-15T05:48:08Z | - |
| dc.date.available | 2016-08-22 | |
| dc.date.copyright | 2011-08-22 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-18 | |
| dc.identifier.citation | 1. 林安邦、范琪艷、李明潤、李一宏、吳龍源與蔡維人（1998）九節木之活血化瘀活性分析。中醫藥雜誌9(4): 239 – 245.
2. 莊榮輝（2005）生物化學課程講義。pp. 4-4 – 4-5. 3. 許榮麒（1993）九節木Psychortia rubra (Lour.) Poir.化學成分之研究。中國醫藥大學中國藥學研究所碩士論文。65頁。 4. 陳定信（2006）實用皮膚醫學，第二版。國立台灣大學醫學院皮膚科部。515頁。 5. 劉和義、楊遠波、呂勝由與施炳霖（2000）台灣維管束植物簡誌 第肆卷。行政院農業委員會，台北。pp. 91-92。 6. 蕭伊妙（2007）維他命C醣苷和熊果素對於 B16 黑色素瘤細胞抑制黑色素生成之研究。國立清華大學化學工程學系碩士論文。52頁。 7. 應紹舜（1998）台灣高等植物彩色圖誌 第六卷。台北。pp. 479 – 483。 8. Ross, M. H. and W. Pawlina (2006) ROSS組織學，第五版。合記圖書出版社，台北。909頁。 9. Benevides, P. J. C., M. C. M. Young, and V. S. Bolzani (2004) Biological activities of constituents from Psychotria spectabilis. Pharm. Biol. 42(8): 565 – 569. 10. Burdock, G. A., M. G. Soni, and I. G. Carabin (2001) Evaluation of health aspects of kojic acid in food. Regul. Toxicol. Pharmacol. 33(1): 80 – 101. 11. Chang, T. S. (2009) An updated review of tyrosinase inhibitors. Int. J. Mol. Sci. 10: 2440 – 2475. 12. Chen, C. H., H. C. Chan, Y. T. Chu, H. Y. Ho, P. Y. Chen, T. H. Lee, and C. K. Lee (2009) Antioxidant activity of some plant extracts towards xanthine oxidase, lipoxygenase and tyrosinase. Molecules 14: 2947 – 2958. 13. Cheng, S. L., L. R. Huang, J. N. Sheu, S. T. Chen, S. Sinchaikul, and G. J. Tsay (2006) Toxicogenomics of kojic acid on gene expression profiling of A375 human malignant melanoma cells. Biol. Pharm. Bull. 29(4): 655 – 669. 14. do Nascimento, N. C., V. Fragoso, D. J. Moura, A. C. e Silva, A. G. Fett-Neto, and J. Saffi (2007) Antioxidant and antimutagenic effects of the crude foliar extract and the alkaloid brachycerine of Psychotria brachyceras. Environ. Mol. Mutagen. 48: 728 – 734. 15. Dojindo Molecular Technologies, INC. (2009) Cell counting kit-8 technical manual. www.dojindo.com 16. Ennes, S. B. P., R. C. Paschoalick, and M. M. de Avelar Alchorne (2000) A double-blind, comparative, placebo-controlled study of the efficacy and tolerability of 4% hydroquinone as a depigmenting agent in melasma. J. Dermatol. Treat. 11(3): 173–179. 17. Espinal-Perez, L. E., B. Moncada, and J. P. Castanedo-Cazares (2004) A double-blind randomized trial of 5% ascorbic acid vs. 4% hydroquinone in melasma. Int. J. Dermatol. 43: 604 – 607. 18. Giang, P. M., H. V. Son, and P. T. Son (2007) Study on the chemistry and antimicrobial activity of Psychotria reevesii Wall. (Rubiaceae). Journal of Chemistry 45(5): 628 – 633. 19. Griffiths, C. E. M., L. J. Finkel, C. M. Ditre, T. A. Hamilton, C. N. Ellis, and J. J. Voorhees (1993) Topical tretinoin (retinoic acid) improves meslasma. A vehicle-controlled, clinical trial. Brit. J. Dermatol. 129: 415 – 421. 20. Gupta, A. K., M. D. Gover, K. Nouri, and S. Taylor (2006) The treatment of melasma: a review of clinical trials. J. Am. Acad. Dermatol. 55(6): 1048 – 1065. 21. Heneka, M. T., P. A. Löschmann, M. Gleichmann, M. Weller, J. B. Schulz, U. Wullner, and T. Klockgether (1998) Induction of nitric oxide synthase and nitric oxide-mediated apoptosis in neuronal PC12 cells after stimulation with tumor necrosis factor-alpha/lipopolysaccharide. J. Neurochem. 71: 88 – 94. 22. Hudnall, P. M. (2000) Hydroquinone. Ullmann's Encyclopedia of Industrial Chemistry, USA. pp. 1 – 8. 23. Hui, W. H. and C. W. Yee (1967) An examination of the Rubiaceae of Hong Kong-III. Phytochemistry 6: 441 – 442. 24. Iwai, K., N. Kishimoto, Y. Kakino, K. Mochida, and T. Fujita (2004) In vitro antioxidative effects and tyrosinase inhibitory activities of seven hydroxycinnamoyl derivatives in green coffee beans. J. Agric. Food Chem. 52: 4893 – 4898. 25. Jia, Z.S., M.C. Tang, and J.M. Wu (1999) The determination of flavonoid contents in mulberry and their scavenging effects on radicals. J. Agric. Food Chem. 64: 555-559. 26. Khan, M. R., M. Kihara, and A. D. Omoloso (2001) Antimicrobial activity of Psychotria microlabastra. Fitoterapia 72: 818 – 821. 27. Kim, Y. J. and H. Uyama (2005) Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future. Cell. Mol. Life Sci. 62: 1707 – 1723. 28. Kujala, T. S., J. M. Loponen, K. D. Klika, and K. Pihlaja (2000) Phenolics and betacyanins in red beetroot (Beta vulgaris) root: distribution and effect of cold storage on the content of total phenolics and three individual compounds. J. Agric. Food Chem. 48: 5338-5342. 29. Lim, T. E. and S. N. Tham (1997) Glycolic acid peels in the treatment of melasma among Asian women. Dermatol. Surg. 23: 17 – 179. 30. Liu, H. Y., T. Y. Yang, S. P. Li, and H. J. Li (1998) Rubiaceae In Flora of Taiwan 4 ( 2nd ed), Taiwan. pp. 315. 31. Locher, C. P., M. T. Burch, H. F. Mower, J. Berestecky, H. Davis, B. Van Poel, A. Lasure, D. A. Vanden Berghe, A. J. Vlietinck (1995) Anti-microbial activity and anti-complement activity of extracts obtained from selected Hawaiian medicinal plants. J. Ethnopharmacol. 49: 23 – 32. 32. Lowe, N. J., D. Rizk, P. Grimes, M. Billips, and S. Pincus (1998) Azelaic acid 20% cream in the treatment of facial hyperpigmentation in darker-skinned patients. Clin. Ther. 20(5): 945 – 959. 33. Maeda, K. and M. Fukuda (1996) Arbutin: Mechanism of its depigmenting action in human melanocyte culture. J. Pharmacol. Exp. Ther. 276: 765 – 769. 34. Maeda, K. and M. Naganuma (1998) Topical trans-4-aminomethylcyclohexane- carboxylic acid prevents ultraviolet radiation-induced pigmentation. J. Photochem. Photobiol. B: Biol. 47: 136 – 141. 35. Masuda, T., D. Yamashita, Y. Takeda, and S. Yonemori (2005) Screening for tyrosinase inhibitors among extracts of seashore plants and identification of potent inhibitors from Garcinia subelliptica. Biosci. Biotechnol. Biochem. 69(1): 197 – 201. 36. Masuda, T., N. Fujuta, Y. Odaka, Y. Takeda, S. Yonemori, K. Nakamoto, and H. Kuninaga (2007) Tyrosinase inhibitory activity of ethanol extracts from medicinal and edible plants cultivated in Okinawa and identification of a water-soluble inhibitor from the leaves of Nandina domestica. Biosci. Biotechnol. Biochem. 71(9): 2316 – 2320. 37. Matsuura, R., H. Ukeda, and M. Sawamura (2006) Tyrosinase inhibitory activity of citrus essential oils. J. Agric. Food Chem. 54: 2309 – 2313. 38. Muhammad, I., D. C. Dunbar, S. I. Khan, B. L. Tekwani, E. Bedir, S. Takamatsu, D. Ferreira, and L. A. Walker (2003) Antiparasitic alkaloids from Psychotria klugii. J. Nat. Prod. 66: 962 – 967. 39. Nelson, D. L. and M. M. Cox (2005) Lehninger principles of biochemistry, 4th Ed. Freeman, New York. pp. 202 – 213. 40. No, J. K., D. Y. Soung, Y. J. Kim, K. H. Shim, Y. S. Jun, S. H. Rhee, T. Yokozawa, and H. Y. Chung (1999) Inhibition of tyrosinase by green tea components. Life Sci. 65(21): 241 – 246. 41. Oyaizu, M. (1986) Antioxidative activity of browning products of glucosamine fractionated by organic solvent and thin-layer chromatography. Nippon Shokuhin Kogyo Gakkaishi. 35: 771. 42. Parvez, S., M. Kang, H. S. Chung, C. Cho, M. C. Hong, M. K. Shin, and H. Bae (2006) Survey and mechanism of skin depigmenting and lightening agents. Phytother. Res. 20: 921 – 934. 43. Perluigi, M. F. D. Marco, C. Foppoli, R. Coccia, C. Blarzino, M. L. Marcante, and C. Cini (2003) Tyrosinase protects human melanocytes from ROS-generating compounds. Biochem. Bioph. Res. Co. 305: 250 – 256. 44. Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. RiceEvans (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine. 26: 1231-1237. 45. Rescigno, A., F. Sollai, B. Pisu, A. Rinadi, and E. Sanjust (2002) Tyrosinase inhibition: general and applied aspects. J. Enzym. Inhib. Med. Chem. 17(4): 207 – 218. 46. Riley, P. A. (1969). Hydroxyanisole depigmentation: in-vivo studies. J. Pathol. 97(2): 185 – 191. 47. Saruno, R. F. Kato, and T. Ikeno (1979) Kojic acid, a tyrosinase inhibitor from Aspergillus albus. Agric. Biol. Chem. 43(6): 1337 – 1338. 48. Seo, S. Y., V. K. Sharma, and N. Sharma (2003) Mushroom tyrosinase: recent prospects. J. Agric. Food Chem. 51: 2837 – 2853. 49. Shimada, K., K. Fujikawa, K. Yahara, and T. Nakamura (1992) Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J. Agric. Food Chem. 40: 945. 50. Shimogaki, H., Y. Tanaka, H. Tamai, and M. Masuda (2000) In vitro and in vivo evaluation of ellagic acid on melanogenesis inhibition. Int. J. Cosmet. Sci. 22: 291 – 303. 51. Slominski, A., D. J. Tobin, S. Shibihara, and J. Wortsman (2003) Melanin pigmentation in mammalian skin and its hormonal regulation. Physiol. Rev. 84: 1155 – 1228. 52. Smit, N., J. Vicanova, and S. Pavel (2009) The hunt for natural skin whitening agents. Int. J. Mol. Sci. 10: 5326 – 5349. 53. Solano, F., S. Briganti, M. Picardo, and G. Ghanem (2006) Hypopigmenting agents: an updated review on biological, chemical and clinical aspects. Pigment Cell Res. 19: 550 – 571. 54. Tan, S. K., C. S. Sim, and C. L. Goh (2008) Hydroquinone-induced exogenous ochronosis in Chinese – two case reports and a review. Int. J. Dermatol. 47(6): 639 – 640. 55. Yoshimura, M., Y. Watanabe, K. Kasai, J. Yamakoshi, and T. Koga (2005) Inhibitory effect of an ellagic acid-rich pomegranate extract on tyrosinase activity. Biosci. Biotechnol. Biochem. 69(12): 2368 – 2373. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47120 | - |
| dc.description.abstract | 本研究以九節木（Psychotria rubra）枝條和葉部乙醇抽出物進行酪胺酸酶抑制效果評估，結果顯示以九節木葉部抽出物乙酸乙酯可溶部的抑制活性最佳，使用酪胺酸與多巴為基質時的IC50值分別為41.04和30.26 μg/ml，活性較目前常用美白成分熊果素佳。以酵素動力學試驗分析得知乙酸乙酯可溶部抑制酪胺酸酶催化黑色素生成的方式，無論使用酪胺酸或多巴為基質時皆為混合型抑制。次分離部中，以G07具有最好的酪胺酸酶抑制效果，使用酪胺酸與多巴為基質時的IC50值分別為46.72和24.00 μg/ml。此外，經細胞毒性試驗發現，九節木葉部抽出物在濃度低於50 μg/ml時，對小鼠黑色素瘤B16-F0細胞無顯著細胞毒性。
分析九節木抽出物與次分離部的抗氧化物質含量，發現以乙酸乙酯可溶部的總酚類含量和總黃酮類含量最高。抗氧化活性評估方面，DPPH自由基捕捉試驗結果顯示以乙酸乙酯可溶部之效果最佳，次分離部中則以G04和G05的自由基捕捉活性最佳，半數抑制濃度分別為5.73和4.75 μg/ml，抑制活性與兒茶素相近。在還原能力與總抗氧化活性評估方面，同樣以乙酸乙酯可溶部及G04∼G09次分離部有最好的活性。 綜合上述結果顯示，九節木葉部乙醇抽出物為良好的酪胺酸酶抑制物並具有極佳的抗氧化能力，值得進一步開發與利用，以提昇九節木的經濟價值。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-15T05:48:08Z (GMT). No. of bitstreams: 1 ntu-100-R98625009-1.pdf: 2638828 bytes, checksum: 0a30f7017ae187abb2230a61bd5637d0 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 摘要 I
Abstract II 目錄 III 圖目錄 VII 表目錄 XII 壹、前言 1 貳、文獻回顧 3 一、黑色素的生成機制 3 (一)酪胺酸酶及其作用機制 3 1. 酪胺酸酶之作用機制 3 2. 酪胺酸酶的基質 4 (二)黑色素生成機制 5 1. 黑色素細胞 5 2. 黑色素之生成機制 6 二、植物天然物的美白機制 9 (一)酪胺酸酶之抑制機制 9 (二)黑色素形成途徑的調控方式 10 1. 抑制黑色素合成的相關酵素活性 10 2. 降低MC1R活性 11 3. 干擾黑色素細小體之成熟與轉移 12 4. 黑色素細胞之死亡 12 三、常見美白成分及其機制 13 (一)植物天然物 13 1. 熊果素 13 2. 沒食子酸及其衍生物 13 3. 鞣花酸 14 4. 維生素C 15 5. 維生素A酸 16 6. 果酸 18 (二)其他化合物 19 1. 對苯二酚及其衍生物 19 2. 麴酸 19 3. 壬二酸 20 4. 傳明酸 21 四、九節木屬植物及其生物活性探討 23 (一)九節木屬植物 23 (二)抗微生物活性 24 (三)抗寄生蟲活性 26 (四)抗腫瘤活性 26 (五)活血化瘀活性 26 (六)抗氧化活性 27 參、材料與方法 29 一、試驗材料 29 (一)試材 29 (二)試驗藥品與溶劑 29 1. 試驗藥品 29 2. 溶劑 29 3. 細胞培養 29 二、試驗方法 30 (一)乙醇抽出物之萃取 30 (二)液相—液相分配萃取 30 (三)管柱層析 30 (四)抑制酪胺酸酶活性之試驗 31 (五)酵素動力學試驗 32 (六)細胞試驗 34 1. 細胞培養 34 2. 細胞計數法 35 3. 細胞毒性試驗 35 (七)抗氧化活性試驗 36 1. 總酚類含量測定試驗 36 2. 總黃酮類含量測定試驗 37 3. DPPH自由基捕捉活性試驗 38 4. 還原能力測定試驗 39 5.總抗氧化活性試驗 39 (八)統計分析 40 肆、結果與討論 41 一、九節木抽出物之酪胺酸酶抑制效果評估 41 (一)九節木枝條與葉部抽出物的酪胺酸酶抑制效果 41 (二)九節木葉部抽出物各可溶部之酪胺酸酶抑制效果評估 45 (三)九節木葉乙酸乙酯次分離部之酪胺酸酶抑制效果評估 48 (四)乙酸乙酯次分離部的酪胺酸酶抑制效果與酵素抑制動力學探討 50 1. 次分離部之酪胺酸酶半數抑制濃度評估 50 2. 次分離部對酵素親和力的影響 53 二、九節木葉部抽出物之細胞毒性評估 62 三、九節木葉部抽出物之抗氧化活性評估 63 (一)總酚類含量分析 63 (二)總黃酮類含量評估 64 (三)DPPH自由基捕捉活性評估 66 (四)還原能力之評估 68 (五)總抗氧化活性評估 69 伍、結論 72 陸、參考文獻 74 | |
| dc.language.iso | zh-TW | |
| dc.subject | 抗氧化活性 | zh_TW |
| dc.subject | 九節木 | zh_TW |
| dc.subject | 酪胺酸酶 | zh_TW |
| dc.subject | 抑制活性 | zh_TW |
| dc.subject | 黑色素 | zh_TW |
| dc.subject | 黑色素瘤細胞 | zh_TW |
| dc.subject | tyrosinase inhibition activity | en |
| dc.subject | antioxidant activity | en |
| dc.subject | melanoma cells | en |
| dc.subject | melanin | en |
| dc.subject | Psychotria rubra | en |
| dc.title | 九節木葉部抽出物抑制黑色素形成與抗氧化活性之研究 | zh_TW |
| dc.title | Studies on Antimelanogenesis Effect and Antioxidant Activities of Psychotria rubra Leaf Extract | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張上鎮,張美鈴 | |
| dc.subject.keyword | 九節木,酪胺酸酶,抑制活性,黑色素,黑色素瘤細胞,抗氧化活性, | zh_TW |
| dc.subject.keyword | Psychotria rubra,tyrosinase inhibition activity,melanin,melanoma cells,antioxidant activity, | en |
| dc.relation.page | 78 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-19 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
| 顯示於系所單位： | 森林環境暨資源學系 | |
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