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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 江伯倫(Bor-Luen Chiang) | |
| dc.contributor.author | Chi-Ping Liao | en |
| dc.contributor.author | 廖季萍 | zh_TW |
| dc.date.accessioned | 2021-06-15T05:47:46Z | - |
| dc.date.available | 2016-08-19 | |
| dc.date.copyright | 2010-09-09 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-08-19 | |
| dc.identifier.citation | 徐凡琪 (2004). 在小鼠氣喘模式中以口服給予Der p 2過敏原誘導抗原特異之耐受性.
陳建彰 (2006). 利用基因轉殖小鼠的氣喘模式研究口服耐受性之機制. 游詩怡 (2007). 探討過敏性氣喘小鼠引發口服耐受性之相關基因. Adkins, B., and Du, R.-Q. (1998). Newborn Mice Develop Balanced Th1/Th2 Primary Effector Responses In Vivo But Are Biased to Th2 Secondary Responses. J Immunol 160, 4217-4224. Ahmad, Z., and Singh, S.K. (2010). Relative and Additional Bronchodilator Response of Salbutamol and Ipratropium in Smoker and Nonsmoker Asthmatics. Journal of Asthma 47, 340-343. Appleman, L.J., and Boussiotis, V.A. (2003a). T cell anergy and costimulation. Immunological Reviews 192, 161-180. Appleman, L.J., and Boussiotis, V.A. (2003b). T cell anergy and costimulation. Immunol Rev 192, 161-180. Arnaboldi, P.M., Roth-Walter, F., and Mayer, L. (2009). Suppression of Th1 and Th17, but not Th2, responses in a CD8+ T cell-mediated model of oral tolerance. Mucosal Immunol 2, 427-438. Benson, J.M., Campbell, K.A., Guan, Z., Gienapp, I.E., Stuckman, S.S., Forsthuber, T., and Whitacre, C.C. (2000). T-cell activation and receptor downmodulation precede deletion induced by mucosally administered antigen. The Journal of Clinical Investigation 106, 1031-1038. Berin, M.C., and Mayer, L. (2008). Immunophysiology of experimental food allergy. Mucosal Immunol 2, 24-32. Bjorksten, B. (1999). Environment and infant immunity. Proceedings of the Nutrition Society 58, 729-732. Bousquet, J. (2008). Efficacy and Safety of Combination Inhaled Corticosteroids/Long-acting β2 -adrenergic Agonists in Single Inhaler Devices for Asthma. European Respiratory Disease Cao, L., Wang, J., Zhu, Y., Tseu, I., and Post, M. (2010). Maternal endotoxin exposure attenuates allergic airway disease in infant rats. Am J Physiol Lung Cell Mol Physiol 298, L670-677. Chen, Y., Kuchroo, V., Inobe, J., Hafler, D., and Weiner, H. (1994). Regulatory T cell clones induced by oral tolerance: suppression ofautoimmune encephalomyelitis. Science 265, 1237-1240. Chirico, G. (2005). Development of the Immune System in Neonates. Journal of the Arab Neonatology Forum 2, 5-11. Cockcroft, D.W., Killian, D.N., Mellon, J.J.A., and Hargreave, F.E. (1977). Bronchial reactivity to inhaled histamine: a method and clinical survey. Clinical & Experimental Allergy 7, 235-243. Coffman, R., Seymour, B., Hudak, S., Jackson, J., and Rennick, D. (1989). Antibody to interleukin-5 inhibits helminth-induced eosinophilia in mice. Science 245, 308-310. Fanta, C.H. (2009). Asthma. N Engl J Med 360, 1002-1014. Fernando, D.M. (1999). Maturation of immune responses at the beginning of asthma. The Journal of allergy and clinical immunology 103, 355-361. Forsthuber, T., Yip, H.C., and Lehmann, P.V. (1996). Induction of TH1 and TH2 Immunity in Neonatal Mice. Science 271, 1728-1730. Fowler, E., and Weiner, H.L. (1997a). Oral tolerance: elucidation of mechanisms and application to treatment of autoimmune diseases. Biopolymers 43, 323-335. Fowler, E., and Weiner, H.L. (1997b). Oral tolerance: Elucidation of mechanisms and application to treatment of autoimmune diseases. Peptide Science 43, 323-335. Friedman, A., and Weiner, H.L. (1994). Induction of anergy or active suppression following oral tolerance is determined by antigen dosage. Proceedings of the National Academy of Sciences of the United States of America 91, 6688-6692. Garside, P., Steel, M., Worthey, E., Kewin, P., Howie, S., Harrison, D., Bishop, D., and Mowat, A. (1996a). Lymphocytes from orally tolerized mice display enhanced susceptibility to death by apoptosis when cultured in the absence of antigen in vitro. Am J Pathol 149, 1971-1979. Garside, P., Steel, M., Worthey, E.A., Kewin, P.J., Howie, S.E., Harrison, D.J., Bishop, D., and Mowat, A.M. (1996b). Lymphocytes from orally tolerized mice display enhanced susceptibility to death by apoptosis when cultured in the absence of antigen in vitro. Am J Pathol 149, 1971-1979. Georas, S.N., Guo, J., De Fanis, U., and Casolaro, V. (2005). T-helper cell type-2 regulation in allergic disease. Eur Respir J 26, 1119-1137. Gerritsen, J., Koeter, G., Postma, D., Schouten, J., and Knol, K. (1989).Prognosis of asthma from childhood to adulthood. Am Rev Respir Dis 140, 1325-1330. Gibson, P.G., Powell, H., and Ducharme, F.M. (2007). Differential effects of maintenance long-acting [beta]-agonist and inhaled corticosteroid on asthma control and asthma exacerbations. Journal of Allergy and Clinical Immunology 119, 344-350. Gonnella, P.A., Waldner, H.P., Kodali, D., and Weiner, H.L. (2004). Induction of low dose oral tolerance in IL-10 deficient mice with experimental autoimmune encephalomyelitis. Journal of Autoimmunity 23, 193-200. Goriely, S., Vincart, B., Stordeur, P., Vekemans, J., Willems, F., Goldman, M., and De Wit, D. (2001). Deficient IL-12(p35) Gene Expression by Dendritic Cells Derived from Neonatal Monocytes. J Immunol 166, 2141-2146. Gounni, A.S., Gregory, B., Nutku, E., Aris, F., Latifa, K., Minshall, E., North, J., Tavernier, J., Levit, R., Nicolaides, N., et al. (2000). Interleukin-9 enhances interleukin-5 receptor expression, differentiation, and survival of human eosinophils. Blood 96, 2163-2171. Hanson, D. (1981). Ontogeny of orally induced tolerance to soluble proteins in mice. I. Priming and tolerance in newborns. J Immunol 127, 1518-1524. Hellman, A., and Chess, A. (2007). Gene Body-Specific Methylation on the Active X Chromosome. Science 315, 1141-1143. Holgate, S.T., and Polosa, R. (2008). Treatment strategies for allergy and asthma. Nat Rev Immunol 8, 218-230. Jianguo Liu, S.C., Lisa M. Herman, Xiaojing Ma (2003). Differential Regulation of Interleukin (IL)-12 p35 and p40 Gene Expression and Interferon (IFN)-γ–primed IL-12 Production by IFN Regulatory Factor 1. Journal of Experimental Medicine 198, 1265-1276. Jones, P.A. (1999). The DNA methylation paradox. Trends in Genetics 15, 34-37. Kalousek, F., and Morris, N.R. (1968). Deoxyribonucleic Acid Methylase Activity in Rat Spleen. Journal of Biological Chemistry 243:, 2440-2442. Kang, B., Kim, K., and Kang, C. (1999). Oral tolerance by a high dose OVA in BALB/c mice is more pronounced and persistent in Th2-mediatedimmune responses than in Th1 responses. Immunobiology 200, 264-276. Kim, J., Samaranayake, M., and Pradhan, S. (2009). Epigenetic mechanisms in mammals. Cellular and Molecular Life Sciences 66, 596-612. Klouwenberg, K., and Bont, P.L. (2008). Neonatal and Infantile Immune Responses to Encapsulated Bacteria and Conjugate Vaccines. Clin Dev Immunol 2008, 628963. Kumar, R.K., Hitchins, M.P., and Foster, P.S. (2009). Epigenetic changes in childhood asthma. Disease Models & Mechanisms 2, 549-553. Laird, P.W. (2010). Principles and challenges of genome-wide DNA methylation analysis. Nat Rev Genet 11, 191-203. Law, J.A., and Jacobsen, S.E. (2010). Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat Rev Genet 11, 204-220. Maegawa, S., Hinkal, G., Kim, H.S., Shen, L., Zhang, L., Zhang, J., Zhang, N., Liang, S., Donehower, L.A., and Issa, J.-P.J. (2010). Widespread and tissue specific age-related DNA methylation changes in mice. Genome Research 20, 332-340. Martinez, F.D., Wright, A.L., Taussig, L.M., Holberg, C.J., Halonen, M., Morgan, W.J., and The Group Health Medical Associates (1995). Asthma and Wheezing in the First Six Years of Life. N Engl J Med 332, 133-138. Mayer, L., and Shao, L. (2004). Therapeutic potential of oral tolerance. Nat Rev Immunol 4, 407-419. McKenzie, A.N.J. (2000). Regulation of T helper type 2 cell immunity by interleukin-4 and interleukin-13. Pharmacology & Therapeutics 88, 143-151. Milgrom, H., Fick, R.B., Su, J.Q., Reimann, J.D., Bush, R.K., Watrous, M.L., Metzger, W.J., and The rhuMAb-E25 Study Group (1999). Treatment of Allergic Asthma with Monoclonal Anti-IgE Antibody. N Engl J Med 341, 1966-1973. Molimard, M., Naline, E., Zhang, Y., Le Gros, V., Begaud, B., and Advenier, C. (1998). Long- and short-acting beta2 adrenoceptor agonists: interactions in human contracted bronchi. Eur Respir J 11, 583-588. Nicolaides, N.C., Holroyd, K.J., Ewart, S.L., Eleff, S.M., Kiser, M.B., Dragwa, C.R., Sullivan, C.D., Grasso, L., Zhang, L.-Y., Messler, C.J., et al.(1997). Interleukin 9: A candidate gene for asthma. Proceedings of the National Academy of Sciences of the United States of America 94, 13175-13180. Ohki, Y., Tokuyama, K., Mayuzumi, H., Sato, A., Koyama, H., Takizawa, T., Arakawa, H., Mochizuki, H., and Morikawa, A. (2005). Characteristic Features of Allergic Airway Inflammation in a Murine Model of Infantile Asthma. International Archives of Allergy and Immunology 138, 51-58. Palmer, M.T., and Weaver, C.T. (2010). Autoimmunity: increasing suspects in the CD4+ T cell lineup. Nat Immunol 11, 36-40. Paul, W.E., and Zhu, J. (2010). How are TH2-type immune responses initiated and amplified? Nat Rev Immunol 10, 225-235. Peng, H.-J., Turner, M.W., and Strobel, S. (1989). Failure to Induce Oral Tolerance to Protein Antigens in Neonatal Mice Can Be Corrected by Transfer of Adult Spleen Cells. Pediatric Research 26, 486-490. Placek, K., Coffre, M., Maiella, S., Bianchi, E., and Rogge, L. (2009). Genetic and epigenetic networks controlling T helper 1 cell differentiation. Immunology 127, 155-162. Ray, A., and Cohn, L. (1999). Th2 cells and GATA-3 in asthma: new insights into the regulation of airway inflammation. The Journal of Clinical Investigation 104, 985-993. Ridge, J.P., Fuchs, E.J., and Matzinger, P. (1996). Neonatal Tolerance Revisited: Turning on Newborn T Cells with Dendritic Cells. Science 271, 1723-1726. Rodenhiser, D., and Mann, M. (2006). Epigenetics and human disease: translating basic biology into clinical applications. CMAJ 174, 341-348. Russo, M., Nahori, M.-A., Lefort, J., Gomes, E., de Castro Keller, A., Rodriguez, D., Ribeiro, O.G., Adriouch, S., Gallois, V., de Faria, A.M.C., et al. (2001). Suppression of Asthma-like Responses in Different Mouse Strains by Oral Tolerance. Am J Respir Cell Mol Biol 24, 518-526. Sakaguchi, S., Yamaguchi, T., Nomura, T., and Ono, M. (2008). Regulatory T Cells and Immune Tolerance. Cell 133, 775-787. Schaub, B., Lauener, R., and von Mutius, E. (2006). The many faces of the hygiene hypothesis. Journal of Allergy and Clinical Immunology 117, 969-977. Scott, P. (1993). IL-12: initiation cytokine for cell-mediated immunity.Science 260, 496-497. Sears, M., Burrows, B., Flannery, E., Herbison, G., Hewitt, C., and Holdaway, M. (1991). Relation between airway responsiveness and serum IgE in children with asthma and in apparently normal children. N Engl J Med 325, 1067-1071. Spear, P.G., and Edelman, G.M. (1974). Maturation of the humoral immune response in mice. The Journal of Experimental Medicine 139, 249-263. Subbarao, P., Mandhane, P.J., and Sears, M.R. (2009). Asthma: epidemiology, etiology and risk factors. CMAJ 181, E181-190. Sunyer, J., Anto, J.M., Sabria, J., Roca, J., Morell, F., Rodriguez-Roisin, R., and Rodrigo, M.J. (1995). Relationship between serum IgE and airway responsiveness in adults with asthma. Journal of Allergy and Clinical Immunology 95, 699-706. Trinchieri, G. (2003). Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat Rev Immunol 3, 133-146. Tsuji, N.M., and Kosaka, A. (2008). Oral tolerance: intestinal homeostasis and antigen-specific regulatory T cells. Trends in Immunology 29, 532-540. Weiner, H.L. (1994). Oral tolerance. Proceedings of the National Academy of Sciences of the United States of America 91, 10762-10765. Weiner, H.L. (2000). Oral tolerance, an active immunologic process mediated by multiple mechanisms. The Journal of Clinical Investigation 106, 935-937. Wills-Karp, M. (1999). immunologic basis of antigen-induced airway hyperresponsiveness. Annual Review of Immunology 17, 255-281. Wills-Karp, M. (2004). Interleukin-13 in asthma pathogenesis. Immunological Reviews 202, 175-190. Zaghouani, H., Hoeman, C.M., and Adkins, B. (2009). Neonatal immunity: faulty T-helpers and the shortcomings of dendritic cells. Trends in Immunology 30, 585-591. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47107 | - |
| dc.description.abstract | 利用口服特定的抗原可以誘導口服耐受性(oral tolerance)的發生，以降低抗原特異性免疫反應。此口服耐受性的現象可以應用於過敏性疾病的治療。過去許多研究發現，輔助T細胞(T helper cells)在幼年時期會傾向於過敏反應的第二型輔助T細胞(Th2)，而IL-12則被普遍認為是參與第一型輔助T細胞的分化有關的細胞激素。另外，有些研究認為DNA 甲基化在附基因調控(epigenetic regulation)中與影響基因的表現及細胞生長分化等功能有關。因此，此研究中，利用氣喘動物模式誘發口服耐受性的機制比較三週齡小鼠和八週齡小鼠之間的差異性。首先，在帶有OVA-T細胞受器(OVA-T cell receptor)基因的基因轉殖小鼠之六至八週齡鼠中成功建立氣喘動物模式。在連續五天口服餵食成鼠OVA 抗原(10 mg/day或100 mg/day)後，結果顯示能有效緩解呼吸道阻力、減輕肺部發炎細胞浸潤現象及降低血清中抗原特異性IgE抗體的產生。然而，相較於八週齡小鼠過敏性氣喘組別，所有的三週齡小鼠皆顯著地降低過敏氣喘現象。此外，三週齡小鼠的脾臟細胞在體外刺激培養後，第二型輔助T細胞細胞激素IL-4的分泌顯著減少，而有些研究推斷幼年時期可能會產生保護性的反應而偏向第一型輔助T細胞(Th1)。然而在IL-12基因甲基化的研究中發現三週齡小鼠與八週齡小鼠於IL-12基因啟動子(promoter)位置皆有甲基化的產生，因此推論IL-12基因啟動子的甲基化可能並非造成小鼠在幼年時期降低過敏反應的主要機制。由本實驗結果推論三週齡的小鼠無法誘發過敏反應可能與免疫系統在不同年齡的成熟度有關，因而造成不同發展的傾向，而更多的推論仍需待更進一步的研究證實。 | zh_TW |
| dc.description.abstract | Oral tolerance has been defined as the lack of systemic immune response that formerly exposed to the same antigen and this characteristic phenomenon might be used as a potential therapeutic approach for allergic diseases, such as asthma. Several studies indicate that T helper cells in early life induce a strong bias towards Th2 function which plays a role in allergic immune response. DNA methylaion is an important epigenetic regulation involved in regulating gene expression and development of cell lineages. Therefore, in this study we would like to compare the different effects on orally delivered antigen to the murine model of asthma between 3-week-old and 8-week-old mice. In this study, we established the animal model of asthma in adult OVA-TCR transgenic mice. Then oral tolerance was induced by feeding 10 mg/day and 100 mg/day of OVA in 8-week-old mice could suppress the response of asthma, such as airway hyperresponsiveness, antigen specific IgE production and eosinophilia, by contrast, were obviously lower than in all 3-week-old mice groups compared to asthmatic group of 8-week-old mice. The levels of IL-4 produced by splenocyte waslower significantly in all 3-week-old groups which probably prone to the protective Th1 response in early life. Whereas methylation of IL-12 gene promoter occurs in either 3-week-old or 8-week-old mice suggested that is not a main mechanism to decrease the
inflammatory response in early life of infant mice. In this study, we demonstrated that 3-week-old mice had less immune response with OVA sensitization. More are needed to clarify the phenomenon, however, mutation of immune system might be different with ages. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T05:47:46Z (GMT). No. of bitstreams: 1 ntu-99-R97450017-1.pdf: 955172 bytes, checksum: 9370de45cc1842e0f11d87eec894739c (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 中文摘要 ………………………………………………………………II
Abstract …………………………………………………………… III Contents ………………………………………………………………V Introduction ……………………………………………………… 1 1 Asthma …………………………………………………………… 2 2 Oral tolerance …………………………………………………… 5 3 Infantile immune response ……………………………………… 9 4 Epigenetic ……………………………………………………… 10 5 Biological role of IL-12 ……………………………………… 11 6 Specific aim of study ………………………………………… 13 Materials and Methods …………………………………………… 14 1. Animal …………………………………………………………… 15 2. Sensitization and challenge of mice ………………………… 15 3. Oral administration of mice ……………………………………………… 16 4. Measurement of airway hyperresponsiveness, AHR ……………………… 16 5. Analysis of the cellular compositions in the bronchoalveolar lavage fluid (BALF) …………………………………………………………………… 17 6. Measurement of cytokine from BALF …………………………………… 17 7. Determination of OVA-specific antibodies in serum …………………… 18 8. Preparation of single cell suspensions of spleen ………………………… 19 9. Antigen-specific splenocyte proliferation ………………………………… 20 10. Determination of cytokines secretion of splenocytes by ELISA …………… 21 11. Isolation of peritoneal macrophage ………………………………………… 21 12. DNA extraction, Bisulfite conversion and PCR (polymerase chain reaction ) …………………………………………………………………… 21 13. Statistical analysis ………………………………………………………… 22 Results ……………………………………………………………………………… 23 1. Establish the murine model of oral tolerance …………………………… 24 2. Oral tolerance in 3-week-old mice ………………………………………… 26 3. DNA methylation on IL-12 gene promoter ……………………………… 30 Discussion …………………………………………………………………………… 32 Figures ……………………………………………………………………………… 39 References ………………………………………………………………………… 63 | |
| dc.language.iso | en | |
| dc.subject | 三週齡小鼠 | zh_TW |
| dc.subject | 介質12 | zh_TW |
| dc.subject | 氣喘 | zh_TW |
| dc.subject | DNA甲基化 | zh_TW |
| dc.subject | 口服耐受性 | zh_TW |
| dc.subject | IL-12 | en |
| dc.subject | 3-week-old mice | en |
| dc.subject | asthma | en |
| dc.subject | DNA methylation | en |
| dc.subject | oral tolerance | en |
| dc.title | 研究幼鼠和成鼠在氣喘動物模式誘發口服耐受性的差異性 | zh_TW |
| dc.title | Study on the Differences between Infant and Adult Mice during the Development of Oral Tolerance in A Murine Model of Allergic Asthma | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李岳倫(Yueh-Lun Lee),郭敏玲(Ming-Ling Kuo) | |
| dc.subject.keyword | 三週齡小鼠,氣喘,DNA甲基化,介質12,口服耐受性, | zh_TW |
| dc.subject.keyword | 3-week-old mice,asthma,DNA methylation,IL-12,oral tolerance, | en |
| dc.relation.page | 69 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-08-19 | |
| dc.contributor.author-college | 牙醫專業學院 | zh_TW |
| dc.contributor.author-dept | 口腔生物科學研究所 | zh_TW |
| 顯示於系所單位： | 口腔生物科學研究所 | |
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