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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47027完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 宋孔彬(Kung-Bin Sung) | |
| dc.contributor.author | Nien-Chung Yu | en |
| dc.contributor.author | 于念中 | zh_TW |
| dc.date.accessioned | 2021-06-15T05:45:35Z | - |
| dc.date.available | 2011-08-20 | |
| dc.date.copyright | 2010-08-20 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-08-19 | |
| dc.identifier.citation | [1] Homola , S. S. Y., GuÈnter Gauglitz (1999). 'Surface plasmon resonance sensors: review.' Sensors and Actuators B 54: 3 - 15
[2] Lin, C.-C., J.-H. Wang, et al. (2010). 'Microfluidic Immunoassays.' Journal of the Association for Laboratory Automation 15(3): 253-274 [3] Nakajima, H., Y. Harada, Y. Asano, T. Nakagama, K. Uchiyama, T. Imato, N. Soh, A. Hemmi (2006). 'A palm-sized surface plasmon resonance sensor with microchip flow cell.' Talanta 70(2): 419-425 [4] 胡品文,應用表面電漿感測器於生物分子作用之動力學研究和構形分析,國立成功大學醫學工程研究所博士論文,中華民國九十五年九月 [5] Wood, R. W (1902). 'On a remarkable case of uneven distribution of light in a diffraction grating spectrum'. Philosophical Magazine 4: 396 [6] Sommerfeld, A ,Ann. Der physik, 28,665-736 [7] Otto, A. (1968) 'Excitation of surface plasma waves in silver by the method of frustrated total reflection'. Z. Physik, 216 ,398–410, [8] Kretschmann, E. H, Raether (1968) ' Radiative decay of nonradiative surface plasmons excited by light '. Z. Naturforsch. A, 23 ,2135–2136 [9] Homola. (2003). 'Present and future of surface plasmon resonance biosensors.' Analytical and Bioanalytical Chemistry 377(3): 528-539 [10] Raether, H. (1988) 'Surface Plasmon on Smooth and Rough Surfaces and on Gratings', Springer-Verlag, Berlin [11] Saleh, B.E.A. , Teich M.C 'Fundamentals of photonic' ,P 253 [12] Homola , I. K., Sinclair S. Yee (1999). 'Surface plasmon resonance sensors based on diffraction gratings and prism couplers: sensitivity comparison.' Sensors and Actuators B 54 16 - 24 [13] Gupta, G. and J. Kondoh (2007). 'Tuning and sensitivity enhancement of surface plasmon resonance sensor.' Sensors and Actuators B: Chemical 122(2): 381-388 [14] Chaiken, I., S. Rose, K.Robert. (1992). 'Analysis of Macromolecular Interactions Using Immobilized Ligands.' Analytical Biochemistry 201(2): 197-210 [15] Glaser, R.W (1993). 'Antigen-Antibody Binding and Mass Transport by Convection and Diffusion to a Surface: A Two-Dimensional Computer Model of Binding and Dissociation Kinetics.' Analytical Biochemistry 213(1): 152-161 [16] Gupta, G., M. Sugimoto,Y. Matsui, J. Kondoh. (2008). 'Use of a low refractive index prism in surface plasmon resonance biosensing.' Sensors and Actuators B: Chemical 130(2): 689-695 [17] Narang, U., P. R. Gauger, S. L. Frances . (1997). 'A Displacement Flow Immunosensor for Explosive Detection Using Microcapillaries.' Analytical Chemistry 69(14): 2779-2785 [18] Dai, Z., S. Serban, H. Ju, N. El Murr. (2007). 'Layer-by-layer hydroxymethyl ferrocene modified sensor for one-step flow/stop-flow injection amperometric immunoassay of α-fetoprotein.' Biosensors and Bioelectronics 22(8): 1700-1706 [19] Panini, N. V., F. A. Bertolino, E.Salinas, G,A, Messina, J. Raba, . (2010). 'Zearalenone determination in corn silage samples using an immunosensor in a continuous-flow/stopped-flow systems.' Biochemical Engineering Journal 51(1-2): 7-13 [20] Aaron, R. W.,C. Soonwoo, R. J. Whelan, R. N. Zare. (2004). 'Poly(dimethylsiloxane) microfluidic flow cells for surface plasmon resonance spectroscopy.' Sensors and Actuators B: Chemical 98(2-3): 208-214 [21] Pharmacia Biosensor AB product Catalog, 1995 [22] E. Stenberg. (1991), 'Quantitative determination of surface concentration of proteins with surface plasmon resonance using radiolabled proteins', J.Colloid .Interf. Sci. 143: 513-526 [23] Thorlab catalogs http://www.thorlabs.com/thorProduct.cfm?partNumber=LJ1622L1 [24] Homola, S. S. Y. (1998). 'Novel polarization control scheme for spectral surface plasmon resonance sensors.' Sensors and Actuators B 51: 331 - 339 [25] Lin C-W (Mar 2010), 'Method for improving surface plasmon resonance byusing conducting metal oxide as adhesive layer' UNITED STATES PATENT AND TRADEMARK OFFICE GRANTED PATENT, patno:US07671995 [26] 留連晉,”表面電漿共振感測系統應用於為奈米流體晶片之蛋白質檢測”國立台灣大學電機工程學系碩士論文,中華民國九十七年七月 [27] http://asemblon.com/node/60/print [28] pierce catalog. http://www.piercenet.con/Produccts/BBrowse.cfm?fldID=0204114&WT.mc_id=keyname [29] Yanase, Y., A. Araki, H. Suzuki, T. Tsutsui, T. Kimura, K. Okamoto, T. Nakatani, T. Hiragun, Hide, Michihiro. (2010). 'Development of an optical fiber SPR sensor for living cell activation.' Biosensors and Bioelectronics 25(5): 1244-1247 [30] Besselink, G. (2004). 'Signal amplification on planar and gel-typesensor surfaces in surface plasmon resonance-based detection of prostate-specific antigen.' Analytical Biochemistry 333(1): 165-173 [31] Ahyai, S. A., M. Graefen, T. Steuber, A. Haese, T. Schlomm, J. Walz, J. Köllermann, A. Briganti, M. Zacharias, M.G. Friedrich. (2008). 'Contemporary Prostate Cancer Prevalence among T1c Biopsy-Referred Men with a Prostate-Specific Antigen Level ≤ 4.0ng per Milliliter.' European Urology 53(4): 750-757 [32] Lee, K.-H., Y.-D. Su, et al. (2007). 'Microfluidic systems integrated with two-dimensional surface plasmon resonance phase imaging systems for microarray immunoassay.' Biosensors and Bioelectronics 23(4): 466-47 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/47027 | - |
| dc.description.abstract | 表面電漿共振系統近年被大量運用於生物分子的感測，由於其具有非標定，高敏感度。利用生物分子免疫分析的特殊選擇性，可以在複雜的混和物中檢測到特定的低濃度蛋白質。再配合微流道的使用，可以提昇蛋白質鍵結能力而觀測較強的訊號。本研究主要在於實際運用一套自行架設的表面電漿共振感測器，從系統的校正，簡易的蛋白質鍵結反應觀測，並比較在流道的使用與否對蛋白質鍵結的差異。同時分析不同的流道環境，對蛋白質鍵結所產生的影響。最後實際分析臨床上具有意義能檢驗出前列腺癌的癌症標記前列腺特異性抗原PSA，並模擬實際在抽血檢測時的檢測環境。 | zh_TW |
| dc.description.abstract | Surface plasmon resonance (SPR) is a kind of optical detecting method and popular to use in biosensor recent years. SPR biosensor has several advantages such as label-free and high sensitivity. Many biomolecules have special selectivity to bind with another biomolecule in complex biological media. Efficiency of binding can be improved by using micro-channel.
In this thesis , we use a home-made SPR system and find its sensitivity and resolution . Compare the difference of conducing immunoassay in micro-channel and on gold thin film. Then, we find the good condition by different flow rate and channel size. At last, we detect the cancer maker protein ,prostate–specific antigen (PSA), and mix PSA in serum to simulation real exsanguinate test. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T05:45:35Z (GMT). No. of bitstreams: 1 ntu-99-R97945022-1.pdf: 1533059 bytes, checksum: 376500156c4e82a2380ef549c1ecdb34 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 致謝 III
中文摘要 IV Abstract V 目錄 VI 圖目錄 VIII 表目錄 XI 第一章 導論 1 1.1研究背景 1 1.2 研究動機及目的 3 1.3本文結構 3 第二章 實驗理論 4 2.1 表面電漿理論背景 4 2.2表面電漿共振現象原理簡介 5 2.3 稜鏡耦合式表面電漿共振原理 9 2.4 相關文獻 14 第三章 實驗材料與方法 17 3.1 實驗系統架設 18 3.2金屬薄膜的選擇與製備 21 3.3 待測樣本的製備 23 3.3.1 蔗糖水溶液及折射率分析 23 3.3.2 待測蛋白質的固化及免疫分析流程 24 3.4 微流道晶片的置備 32 3.5表面電漿共振訊號量測方式與分析方法 34 第四章 實驗結果與討論 36 4.1 系統效能測試及分析 36 4.2微流道晶片的樣本訊號分析結果 39 4.2.1普通金膜晶片上的蛋白質樣本訊號分析結果 39 4.2.2微流道晶片上的蛋白質樣本訊號分析結果 41 4.3不同吸附層金膜的表面電漿共振差異 45 4.4不同微流道參數條件下的免疫分析結果比較 47 4.5癌症標記蛋白質樣本訊號分析結果 52 4.5.1 純化癌症標記蛋白樣本分析 52 4.5.2 複雜環境中的蛋白質樣本分析 56 第五章 結論與未來發展 59 參考文獻 60 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用表面電漿共振感測微米流體晶片中的蛋白質免疫分析反應 | zh_TW |
| dc.title | Using surface plasmon resonance sensing for protein immunoassay in a micro-fluidic chip | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林啟萬(Chii-Wann Lin),林致廷(Chih-Ting Lin) | |
| dc.subject.keyword | 表面電漿共振,生物分子感測器,免疫分析,微米流道晶片, | zh_TW |
| dc.subject.keyword | surface plasmon resonance,biosensor,immunoassay,micro-channel chip, | en |
| dc.relation.page | 63 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-08-19 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
| 顯示於系所單位： | 生醫電子與資訊學研究所 | |
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| ntu-99-1.pdf 目前未授權公開取用 | 1.5 MB | Adobe PDF |
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