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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳俊任(Chun-Jen Chen) | |
dc.contributor.author | Jing-Huei Huang | en |
dc.contributor.author | 黃靖惠 | zh_TW |
dc.date.accessioned | 2021-06-15T05:44:28Z | - |
dc.date.available | 2012-08-20 | |
dc.date.copyright | 2010-08-20 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-19 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46976 | - |
dc.description.abstract | 自體免疫性肝炎(autoimmune hepatitis, AIH)是一種慢性肝炎,好發於年輕女性,且可能進展為肝硬化。罹患自體免疫性肝炎時,患者的免疫系統會以肝臟細胞為攻擊對象,持續地破壞肝臟。目前對於造成自體免疫性肝炎的機制尚不清楚,本研究以 Con A 建立自體免疫肝炎的模式,探討 tumor necrosis factor-α (TNF-α)及 IL-1 (interleukin-1) 在自體免疫性肝炎扮演的機制。本研究結果發現,在TNF-α-/-、TNFR1-/- 及 IL-1R-/- 小鼠,嗜中性球進入肝組織的情形會減少,並且肝受損指數 ALT 有顯著下降。因此結果顯示,Con A 造成小鼠的肝損傷會透過TNF-α及 IL-1 的參與,吸引嗜中性球進入肝組織,造成肝組織受損。在本研究也發現在 TNF-α-/-及 TNFR1-/- 小鼠,CD4+ T 細胞浸潤肝臟組織會減少。因此 Con A
會透過 TNF-α 的參與,誘發 CD4+ T 細胞浸潤肝臟組織,進而造成肝臟的損傷。 酒精性肝炎引起的病理特徵有脂肪肝、發炎及肝細胞壞死。但對於酒精如何 引起肝臟受損的機制並不清楚。因此本研究建立急性酒精性肝炎的模式,探討其 可能的機制。研究中發現,在TNFR1 缺陷小鼠中,嗜中性球浸潤肝臟組織的情形 會減少,且肝受損指數 ALT 及 AST 有顯著下降。本研究的結果證實在酒精誘發 的急性肝損傷模式中 TNFα-TNFR1 之作用途徑參與嗜中性球浸潤肝臟組織,以及 造成肝組織受損情形。 | zh_TW |
dc.description.abstract | Autoimmune hepatitis is a chronic inflammation in the liver that occurs when the body‘s immune system attacks the liver. Although the cause of autoimmune hepatitis is
not entirely clear, some diseases, toxins and drugs may trigger autoimmune hepatitis insusceptible people, especially in women. Concanavalin A (Con A)-induced hepatitis in mice is thought to mimic autoimmune hepatitis in human. In this study, we investigated the role of TNF-α and IL-1 in Con A-induced hepatitis. We found that Con A-triggered serum ALT and AST production and neutrophil infiltration in liver were markedly reduced in TNF-α-/- mice, TNFR1-/- mice and IL-1R-/- mice. Furthermore, the number of CD4+ T cells in liver was also reduced in mice deficient of TNF-α, and TNFR1. Overall our findings imply that both TNF-α and IL-1 play important roles in mediating Con A-induced hepatitis by regulating the recruitment of neutrophils into liver. Although the histopathology of early alcoholic liver disease, i.e., steatosis,inflammation, and necrosis, has been well documented, the exact mechanisms of pathogenesis of this devastating disease are still unknown. In this study, we established the model of acute-alcohol hepatitis to investigate the role of TNF-α in ethanol-induced acute liver injury. We found that serum ALT levels in TNFR1-deficient mice were markedly reduced in alcohol-induced liver Injury. Neutrophil infiltration in alcohol-induced liver injury was also reduced in TNFR1-deficient mice. These results demonstrated that TNF-α was involved in alcohol-induced hepatitis by mediating the recruitment of neutrophils into liver. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T05:44:28Z (GMT). No. of bitstreams: 1 ntu-99-R97b47120-1.pdf: 2521552 bytes, checksum: 5e179230daeb9f55e10c46dca3a54e44 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 中文摘要 ............................................................................................................... iii
Abstract .................................................................................................................. iv Abbreviation .......................................................................................................... vi 第一章 緒論 .......................................................................................................... 1 1.1 肝炎的現況 .................................................................................................... 1 1.1.1 病毒性肝炎 ......................................................................................... 1 1.1.2 非病毒肝炎 ........................................................................................ 1 1.2 肝臟構造及細胞組成 .................................................................................... 2 1.2.1 肝細胞 ................................................................................................. 3 1.2.2 內皮細胞( endothelial cell) ................................................................. 3 1.2.3 庫氏細胞 (Kupffer cell) ..................................................................... 3 1.2.4 淋巴細胞 ............................................................................................. 3 1.3 發炎反應 ..................................................................................................... 4 1.3.1 急性發炎反應 ...................................................................................... 4 1.3.2 慢性發炎反應 ..................................................................................... 5 1.4 參與發炎反應之細胞 ............................................................................... 5 1.4.1 巨噬細胞 .............................................................................................. 5 1.4.2 單核球細胞 ......................................................................................... 5 1.4.3 嗜中性球 .............................................................................................. 6 1.4.4 肥大細胞 (mast cell) ........................................................................... 6 1.5 參與發炎反應的細胞激素及接受器 ...................................................... 6 1.5.1 TNF-α ................................................................................................... 6 1.5.2 IL-6 ....................................................................................................... 7 1.5.3 IFN-γ ..................................................................................................... 7 ix 1.5.4 MCP-1 (monocyte chemoattractant protein 1) ..................................... 7 1.5.5 KC ......................................................................................................... 8 1.5.6 IL-1 ....................................................................................................... 8 1.6 Concanavalin A (Con A) 誘發小鼠急性肝炎 ................................................ 8 1.7 酒精性肝炎 .................................................................................................... 9 1.8 實驗目的 ......................................................................................................... 9 1.8.1 Concanavalin A (Con A) 誘發小鼠急性肝炎模式 ........................... 10 1.8.2 誘導小鼠急性酒精肝炎模式 ............................................................ 10 第二章 材料與方法 ............................................................................................ 11 2.1 以Con A 誘發小鼠急性肝炎 ...................................................................... 11 2.1.1 動物種類 ............................................................................................ 11 2.1.2 Con A 誘發小鼠急性肝炎 ................................................................ 11 2.1.3 分析 ALT 及 AST .......................................................................... 11 2.1.4 Myeloperoxidase (MPO) activity assay .............................................. 11 2.1.5 蘇木紫 (hematoxylin)及伊紅 (eosin)免疫組織染色觀察 (H&E 染色) ..................................................................................................................... 12 2.1.6 免疫細胞浸潤分析 (immune cells infiltrate assay) ......................... 12 2.1.7 分析肝臟組織中的細胞激素 ........................................................... 13 2.1.8 血清中細胞激素的釋放 ................................................................... 13 2.1.9 以流式細胞儀探討 CD4+ T 細胞、CD8+ T 細胞浸潤肝臟的情形14 2.2 以酒精誘發小鼠急性肝損傷 ....................................................................... 15 第三章 研究結果 ................................................................................................ 16 3.1. TNF-α 在Con A 誘發小鼠急性肝炎模式扮演之角色 ............................. 16 3.1.1 小鼠肝損傷情形 ................................................................................ 16 3.1.2 嗜中性球浸潤肝臟的情形 ............................................................... 16 x 3.1.3 在 Con A 誘導小鼠急性肝炎,對血清中及肝組織中參與發炎反應 之細胞激素產生的影響 ............................................................................. 17 3.1.4 在 Con A 誘導小鼠急性肝炎,對血清中及肝組織中參與發炎反應 之趨化因子產生的影響 ............................................................................. 18 3.1.5 探討浸潤肝臟免疫細胞的情形 ..................................................... 18 3.1.6 以Enbrel 抑制TNF-α 作用,探討對於Con A 引起的肝炎的影響19 3.2 IL-1R 在Con A 誘發小鼠急性肝炎模式扮演之角色................................ 19 3.2.1 小鼠肝損傷情形-血清中ALT 活性表現 ......................................... 19 3.2.2 嗜中性球浸潤肝臟的情形-MPO 活性表現 ................................... 20 3.2.3 小鼠以 Con A 刺激後,肝組織中細胞激素產生的情形 ............. 20 3.3 酒精誘發小鼠急性肝炎模式 ....................................................................... 20 3.3.1 建立酒精誘發小鼠急性肝炎模式 ................................................... 20 3.3.2 血清中ALT、AST 的活性.............................................................. 21 3.3.3 肝組織中骨髓過氧化酶 ( Myeloperoxidase, MPO )的表現 .......... 21 第四章 討論 ........................................................................................................ 22 4.1 探討Con A 引起肝受損的機制 .................................................................. 22 4.2 酒精性肝炎 .................................................................................................. 25 參考文獻 .............................................................................................................. 27 | |
dc.language.iso | zh-TW | |
dc.title | TNF-α 及IL-1 分別在Concanavalin A 及酒精誘導的
急性肝炎所扮演之角色 | zh_TW |
dc.title | The role of TNF-α and IL-1 in Con A-induced hepatitis
and acute alcohol-induced hepatitis | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 許秉寧(Ping-Ning Hsu),陳念榮(Nien-Jung Chen) | |
dc.subject.keyword | 嗜中性球,巨噬細胞,刀豆素,發炎反應,單核球, | zh_TW |
dc.subject.keyword | Concanavalin A,tumor necrosis factor (TNF)-α,inflammation, | en |
dc.relation.page | 55 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-08-19 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
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