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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 趙本秀(Pen-Hsiu Chao) | |
| dc.contributor.author | Shih-Fan Yu | en |
| dc.contributor.author | 游仕帆 | zh_TW |
| dc.date.accessioned | 2021-06-15T05:42:38Z | - |
| dc.date.available | 2011-08-24 | |
| dc.date.copyright | 2010-08-24 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-08-20 | |
| dc.identifier.citation | 1. Chao, P.-h. G., Lu, H. H., Hung, C. T., Nicoll, S. B. & Bulinski, J. C. (2007). Effects of Applied DC Electric Field on Ligament Fibroblast Migration and Wound Healing. Connective Tissue Research 48, 188-197.
2. Van de Velde, S. K., DeFrate, L. E., Gill, T. J., Moses, J. M., Papannagari, R. & Li, G. (2007). The effect of anterior cruciate ligament deficiency on the in vivo elongation of the medial and lateral collateral ligaments. Am J Sports Med 35, 294-300. 3. Boden, B. P., Griffin, L. Y. & Garrett, W. E., Jr. (2000). Etiology and Prevention of Noncontact ACL Injury. Phys Sportsmed 28, 53-60. 4. Kadler, K. E., Holmes, D. F., Trotter, J. A. & Chapman, J. A. (1996). Collagen fibril formation. Biochem J 316 1-11. 5. Gelse K, P. E., Aigner T. (2000). Collagens--structure, function, and biosynthesis. Advanced Drug Delivery Reviews 55, 1531-1546. 6. Weiss, J. A., Woo, S. L.-Y., Ohland, K. J., Horibe, S. & Newton, P. O. (1991). Evaluation of a new injury model to study medial collateral ligament healing: Primary repair versus nonoperative treatment. Journal of Orthopaedic Research 9, 516-528. 7. Wang, J. H. C., Jia, F., Gilbert, T. W. & Woo, S. L. Y. (2003). Cell orientation determines the alignment of cell-produced collagenous matrix. Journal of Biomechanics 36, 97-102. 8. Brunette, D. M. (1986). Fibroblasts on micromachined substrata orient hierarchically to grooves of different dimensions. Experimental Cell Research 164, 11-26. 9. Baker, B. M. & Mauck, R. L. (2007). The effect of nanofiber alignment on the maturation of engineered meniscus constructs. Biomaterials 28, 1967-1977. 10. Rajnicek, A. M., Foubister, L. E. & McCaig, C. D. (2006). Temporally and spatially coordinated roles for Rho, Rac, Cdc42 and their effectors in growth cone guidance by a physiological electric field. J Cell Sci 119, 1723-1735. 11. Rajnicek, A. M., Foubister, L. E. & McCaig, C. D. (2008). Alignment of corneal and lens epithelial cells by co-operative effects of substratum topography and DC electric fields. Biomaterials 29, 2082-2095. 12. Choi, J. S., Lee, S. J., Christ, G. J., Atala, A. & Yoo, J. J. (2008). The influence of electrospun aligned poly([var epsilon]-caprolactone)/collagen nanofiber meshes on the formation of self-aligned skeletal muscle myotubes. Biomaterials 29, 2899-2906. 13. Ferrier, J., Ross, S. M., Kanehisa, J. & Aubin, J. E. (1986). Osteoclasts and osteoblasts migrate in opposite directions in response to a constant electrical field. Journal of Cellular Physiology 129, 283-288. 14. Zhao, M., McCaig, C. D., Agius-Fernandez, A., Forrester, J. V. & Araki-Sasaki, K. (1997). Human corneal epithelial cells reorient and migrate cathodally in a small applied electric field. Current Eye Research 16, 973 - 984. 15. Lohmann, C. H., Schwartz, Z., Liu, Y., Guerkov, H., Dean, D. D., Simon, B. & Boyan, B. D. (2000). Pulsed electromagnetic field stimulation of MG63 osteoblast-like cells affects differentiation and local factor production. Journal of Orthopaedic Research 18, 637-646. 16. Cooper, M. S. & Keller, R. E. (1984). Perpendicular orientation and directional migration of amphibian neural crest cells in dc electrical fields. Proc Natl Acad Sci U S A 81, 160-4. 17. Zhao M, F. J., McCaig CD. (1999). A small, physiological electric field orients cell division. Proc Natl Acad Sci USA 96, 4942-4946. 18. Chao, P.-H. G., Roy, R., Mauck, R. L., Liu, W., Valhmu, W. B. & Hung, C. T. (2000). Chondrocyte Translocation Response to Direct Current Electric Fields. Journal of Biomechanical Engineering 122, 261-267. 19. Aaron, R. K., Ciombor, D. M. & Simon, B. J. (2004). Treatment of nonunions with electric and electromagnetic fields. Clin Orthop Relat Res, 21-9. 20. Peters, E. J., Lavery, L. A., Armstrong, D. G. & Fleischli, J. G. (2001). Electric stimulation as an adjunct to heal diabetic foot ulcers: A randomized clinical trial. Archives of Physical Medicine and Rehabilitation 82, 721-725. 21. Tandon, N., Cannizzaro, C., Chao, P. H., Maidhof, R., Marsano, A., Au, H. T., Radisic, M. & Vunjak-Novakovic, G. (2009). Electrical stimulation systems for cardiac tissue engineering. Nat Protoc 4, 155-73. 22. Date H, F. T., Sakoma Y, Yoshida A, Hayashi Y, Abe N, Ozaki T. (2009). GDF-5/7 and bFGF activate integrin alpha2-mediated cellular migration in rabbit ligament fibroblasts. J Orthop Res, 28, 225-231. 23. Burgess, B. T., Myles, J. L. & Dickinson, R. B. (2000). Quantitative Analysis of Adhesion-Mediated Cell Migration in Three-Dimensional Gels of RGD-Grafted Collagen. Annals of Biomedical Engineering 28, 110-118. 24. http://www.taosortho.com/patientinfo/Medical/knees/ACL.htm | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46879 | - |
| dc.description.abstract | 十字韌帶具有穩定關節功用，因癒合能力極小，而導致日後關節疼痛。本實驗利用外加電場促進十字韌帶癒合。在正常生理上細胞遷移扮演重要角色，包括傷口癒合以及型態發生。使用豬膝關節韌帶細胞外加電場，研究細胞在自然的基質遷移及纖維排列走向影響。我們發現纖維走向和電場會影響到細胞的移動。細胞型態在沒有電場時方向性是呈現任意走向，纖維排列與電場方向垂直細胞型態呈現圓形。我們進一步利用排列膠原蛋白模擬基質的結構，膠原蛋白纖維和電場垂直時會降低細胞的遷移。因此在纖維排列上確實會造成細胞移動的影響。 | zh_TW |
| dc.description.abstract | Electric fields (EFs) induce and enhance cell migration in various tissues. We applied electric field to enhance the migration of the ligament fibroblasts on its native matrix, and the alignment of the collagen fibers also affects the migration of the fibroblasts. Cell migration plays an important role in normal physiological activities, including wound healing and morphogenesis. Cruciate ligament is mainly used to stabilize the knee joint, and ligaments are the most common injury sites. When subject to applied EF, ligament fibroblasts enhance migration along their native matrix when fibers were oriented parallel to the direction of the field. We further simplify ligament structure to use collagen alignment to test structure effect. When collagen fibers were oriented perpendicular to the direction of the field, cells migrate slowly. Therefore, collagen fiber alignment effects cells migration. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T05:42:38Z (GMT). No. of bitstreams: 1 ntu-99-R97548020-1.pdf: 617844 bytes, checksum: ab4be609b811aa3adab64414b6a3a56f (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 中文摘要....................4
英文摘要.................5 Introduction................6 Materials and methods................12 Results....................16 Discussion.................20 Reference...................24 附錄...................26 | |
| dc.language.iso | en | |
| dc.subject | 韌帶 | zh_TW |
| dc.subject | 膠原纖維排列 | zh_TW |
| dc.subject | 電刺激 | zh_TW |
| dc.subject | collagen fiber alignment | en |
| dc.subject | electric field | en |
| dc.subject | knee ligament | en |
| dc.title | 細胞外基質排列以及電場對韌帶細胞遷移的影響 | zh_TW |
| dc.title | Effects of ECM alignment on migration of ligament fibroblast with applied DC EF | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 楊台鴻(Tai-Horng Young),朱士維(Shi-Wei Chu) | |
| dc.subject.keyword | 電刺激,韌帶,膠原纖維排列, | zh_TW |
| dc.subject.keyword | electric field,knee ligament,collagen fiber alignment, | en |
| dc.relation.page | 34 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-08-20 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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