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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 江俊斌,郭彥彬 | |
| dc.contributor.author | Yi-Ching Liu | en |
| dc.contributor.author | 柳依青 | zh_TW |
| dc.date.accessioned | 2021-06-15T05:41:29Z | - |
| dc.date.available | 2012-10-05 | |
| dc.date.copyright | 2011-10-05 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-04 | |
| dc.identifier.citation | lthoefer H, Eversole-Cire P, Simon MI: Constitutively active Galphaq and Galpha13 trigger apoptosis through different pathways. J Biol Chem 272(39): 24380-6, 1997
Ballantyne AJ, McCarten AB, Ibanez ML: The extension of cancer of the head and neck through peripheral nerves, Am J Surg 106:657-67, 1963 Barrett K, Leptin M, Settleman J.: The Rho GTPase and a putative RhoGEF mediate a signaling pathway for the cell shape changes in Drosophila gastrulation. Cell 91(7): 905–15, 1997 Bureau of Health Promotion, Department of Health, R.O.C(行政院衛生署國民健康局癌症登記): https://cris.bhp.doh.gov.tw/pagepub/Home.aspx Berestetskaya YV, Faure MP, Ichijo H, Voyno-Yasenetskaya TA: Regulation of apoptosis by alpha-subunits of G12 and G13 proteins via apoptosis signal-regulating kinase-1, J Biol Chem 273 (43): 27816–23, 1998 Bernier J, Domenge C, Ozsahin M, et al: Postoperative irradiation with or without concomitant chemotherapy for locally advanced head and neck cancer, N Engl J Med 350:1945–1952, 2004 Bernier J, Cooper JS, et al: Defining risk levels in locally advanced head and neck cancers: a comparative analysis of concurrent postoperative radiation plus chemotherapy trials of the EORTC(#22931) and RTOG (#9501), Head Neck 27: 843– 50, 2005 Bian D, Mahanivong C, Yu J, Frisch SM, Pan ZK, Ye RD et al: The G12/13-RhoA signaling pathway contributes to efficient lysophosphatidic acid-stimulated cell migration. Oncogene 25 (15): 2234–44, 2006 Bouquot JE, Gnepp DR: Laryngeal precancer- a review of the literature, commentary and comparison with oral luekoplakia, Head Neck 13:488-97,1991 Buhl AM, Johnson NL, Dhanasekaran N, Johnson GL: G alpha 12 and G alpha 13 stimulate Rho-dependent stress fiber formation and focal adhesion assembly. J Biol Chem 270 (42): 24631–4,1995 Chan AM, Fleming TP, McGovern ES, Chedid M, Miki T, Aaronson SA: Expression cDNA cloning of a transforming gene encoding the wild-type G alpha 12 gene product, Mol Cell Biol 13 (2): 762–8,1993 Collins LR, Minden A, Karin M, Brown JH: Galpha12 stimulates c-Jun NH2-terminal kinase through the small G proteins Ras and Rac. J Biol Chem 271 (29): 17349–53,1996 Chen CJ et al: Cancer Epidemiology and control in Taiwan: A brief review, Jpn J Clin Oncol 32:S66-81, 2002 Chen YJ, Chang J. TC, Liao CT, Wang HM, Yen TC, Chiu CC, Lu YC, Li HS ,Cheng AJ et al: Head and neck cancer in the betel quid chewing area: recent advances in molecular carcinogenesis, Cancer Sci 99(8): 1507-14, 2008 Chen YK, Huang HC, Lin LM, Lin CC: Primary oral squamous cell carcinoma: an analysis of 703 cases in southern Taiwan, Oral Oncol 35(2): 173-9, 1999 Conacci-Sorrell M, Zhurinsky J, Ben-Ze’ev A: The cadherincatenin adhesion system in signaling and cancer. J Clin Invest 109 (8): 987–991,1993 Cooper JS, Pajak TF, Forastiere AA, et al: Postoperative concurrent radiotherapy and chemotherapy for high-risk squamous-cell carcinoma of the head and neck, N Engl J Med 350:1937–44, 2004 Dermott JM, Reddy MR, Onesime D, Reddy EP, Dhanasekaran N: Oncogenic mutant of Galpha12 stimulates cell proliferation through cycloxygenase-2 signaling pathway. Oncogene 18 (51): 7185–7189, 1999 Dhanasekaran N, Tsim ST, Dermott JM, Onesime D: Regulation of cell proliferation by G proteins. Oncogene 17: 1383–1394,1998 Dickenson AJ, Currie WJ, Avery BS: Screening for syphilis in patients with carcinoma of tongue, Br J Oral Maxillofac Surg 33:319-20, 1995 Diviani D, Soderling J, Scott JD (2001). AKAP-Lbc anchors protein kinase A and nucleates Galpha 12-selective Rho-mediated stress fiber formation. J Biol Chem 276 (47): 44247–57,2001 Dolezalova et al.: Biochemical Regulation of Breast Cancer Cell Expression of S1P2 (Edg-5) and S1P3 (Edg-3) G Protein-Coupled Receptors for Sphingosine 1-Phosphate, J. Cell. Biochem. 88(4):732–43 ,2003 Dorsam RT, Gutkind JS: G-protein-coupled receptors and cancer. Nat Rev Cancer 7 (2): 79–94,2007 Etienne-Manneville S, Hall A: Rho GTPases in cell biology. Nature 420 (6916): 629–35, 2002 Even-Ram S, Uziely B, Cohen P, Grisaru-Granovsky S, Maoz M, Ginzburg Y et al: Thrombin receptor overexpression in malignant and physiological invasion processes. Nat Med 4 (8): 909–14,1998 Flaitz CM, Hicks MJ: Molecular piracy: the viral link to carcinogenesis, Oral Oncol 34:448-53, 1998 Friedl P, Wolf K: Tumour-cell invasion and migration: diversity and escape mechanisms, Nat Rev Cancer 3 (5): 362–74,2003 Fritz G, Just I, Kaina B: Rho GTPases are over-expressed in human tumors, Int J Cancer 81 (5): 682–7,1999 Fromm C, Coso OA, Montaner S, Xu N, Gutkind JS:The small GTP-binding protein Rho links G protein-coupled receptors and Galpha12 to the serum response element and to cellular transformation, Proc Natl Acad Sci USA 94 (19): 10098–103,1997 Fukuhara S, Murga C, Zohar M, Igishi T, Gutkind JS: A novel PDZ domain containing guanine nucleotide exchange factor links heterotrimeric G proteins to Rho, J Biol Chem 274 (9): 5868–5879,1999 Fukuhara S, Marinissen MJ, Chiariello M, Gutkind JS: Signaling from G protein-coupled receptors to ERK5/Big MAPK 1 involves Galpha q and Galpha 12/13 families of heterotrimeric G proteins. Evidence for the existence of a novel Ras AND Rho-independent pathway, J Biol Chem 275 (28): 21730–21736,2000b Gohla A, Offermanns S, Wilkie TM, Schultz G: Differential involvement of Galpha12 and Galpha13 in receptor-mediated stress fiber formation, J Biol Chem 274 (25): 17901–7,1999 Gohla A, Schultz G, Offermanns S: Role for G(12)/G(13) in agonist-induced vascular smooth muscle cell contraction, Circ Res 87 (3): 221–227,2000 Gold KA, Lee HY, Kim ES: Targeted therapies in squamous cell carcinoma of the head and neck, Cancer; 115:922–35, 2009 Goldsmith ZG, Dhanasekaran DN: G protein regulation of MAPK networks, Oncogene 26 (22): 3122–3142,2007 Goulimari P, Kitzing TM, Knieling H, Brandt DT, Offermanns S, Grosse R: Galpha12/13 is essential for directed cell migration and localized Rho-Dia1 function. J Biol Chem 280 (51): 42242–51,2005 Gu JL, Muller S, Mancino V, Offermanns S, Simon MI: Interaction of G alpha(12) with G alpha(13) and G alpha(q) signaling pathways, Proc Natl Acad Sci USA 99 (14): 9352–7,2002 Henrikson KP, Salazar SL, Fenton JW II, Pentecost BT: Role of thrombin receptor in breast cancer invasiveness, Br J Cancer 79 (3–4): 401–6, 1999 Hersch E, Huang J, Grider JR, Murthy KS:Gq/G13 signaling by ET-1 in smooth muscle: MYPT1 phosphorylation via ETA and CPI-17 dephosphorylation via ETB, Am J Physiol Cell Physiol 287(5): C1209–18, 2004 Hitoshi Kurose: Ga12 and Ga13 as key regulatory mediator in signal transduction, Life Sciences 74: 155–61, 2003 Howard A. Rockman, Walter J. Koch and Robert J. Lefkowitz: Seven-transmembrane-spanning receptors and heart function, Nature 405: 206-12, 2002 Iguchi T, Sakata K, Yoshizaki K, Tago K, Mizuno N, Itoh H: Orphan G protein-coupled receptor GPR56 regulates neural progenitor cell migration via a G alpha 12/13 and Rho pathway, J Biol Chem 283 (21): 14469–78,2008 JJ Lee, HC hung, SJ Cheng, et al: Carcinoma and dysplasia in oral leukoplakias in Taiwan: Prevalence and risk factors, Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:472-80, 2006 J Juneja and PJ Casey: Role of G12 proteins in oncogenesis and metastasis, Br. J. Pharmacol 158:32-40, 2009 Kawalski LP, Bagietto R, Lara JR, et al: Prognostic significance of the distribution of neck node metastasis from oral carcinoma Head Neck 22:207-14, 2000 Kelly P, Moeller BJ, Juneja J, Booden MA, Der CJ, Daaka Y et al: The G12 family of heterotrimeric G proteins promotes breast cancer invasion and metastasis, Proc Natl Acad Sci USA 103(21): 8173–8, 2006a Kelly P, Stemmle LN, Madden JF, Fields TA, Daaka Y, Casey PJ A role for the G12 family of heterotrimeric G proteins in prostate cancer invasion, J Biol Chem 281 (36): 26483–90,2006b Kelly P, Casey PJ, Meigs TE: Biologic functions of the G12 subfamily of heterotrimeric G proteins: growth, migration, and metastasis, Biochemistry 46 (23): 6677–87, 2007 Kitzing TM, Sahadevan AS, Brandt DT, Knieling H, Hannemann S, Fackler OT et al: Positive feedback between Dia1, LARG, and RhoA regulates cell morphology and invasion, Genes Dev 21 (12): 1478–83, 2007 Ko YC, Huang YL, Lee CH et al: Betal quid chewing, smoking and alcohol consumption realted to oral cancer in Taiwan, J Oral Pathol Med 24: 450-3, 1995 Krogh P, Hald B, Holmstrup P: Possible mycological etiology of oral mucosal cancer. Catalytic potential of infecting Candida albicans and other yeasts in production of N-nitrosobenzylmethylamine, Carcinogenesis 8:1543-8, 1987 Kumar RN, Ha JH, Radhakrishnan R, Dhanasekaran DN: Transactivation of platelet-derived growth factor receptor alpha by the GTPase-deficient activated mutant of Galpha12, Mol Cell Biol 26(1): 50–62,2006a Kumar RN, Shore SK, Dhanasekaran N : Neoplastic transformation by the gep oncogene, Galpha12, involves signaling by STAT3, Oncogene 25 (6): 899–906, 2006b Kurose H:Galpha12 and Galpha13 as key regulatory mediator in signal transduction. Life Sci 74 (2–3): 155–61, 2003 Leemans CR, Boudewijn J. M. Braakhuis and Ruud H. Brakenhoff: The molecular biology of head and neck cancer, Rev Nat Cancer 11:9-22, 2011 Llewellyn CD, Linklater K, Bell J, Johns NW, Warnakulasuriya S :An analysis of risk factors for oral cancer in young people: a case-control study, Oral Oncol, 40(3): 304-13, 2004 Liao CT, Chang J. CT, Wang HM, Yen TC et al: Analysis of risk factors of predictive local tumor control in oral cavity cancer, annals of surgical oncology 15(3):915–922, 2008 Liu SC, Jen YM, Jiang SS, et al: Nasopharyngeal Carcinoma by Modulating Actin Ga12-Mediated Pathway Promotes Invasiveness of Cytoskeleton Reorganization, Cancer Res 69:6122-6130,2009 Machtay et al: Factors Associated With Severe Late Toxicity After Concurrent Chemoradiation for Locally Advanced Head and Neck Cancer: An RTOG Analysis, J Clin Oncol 26:3582-3589, 2008 Marchese A, George S, Kolakowski LF, Lynch KR, O’Dowd BF: Novel GPCRs and their endogenous ligands: expanding the boundariesof physiology and pharmacology, TiPS : 370-5, 1999 Marinissen MJ, Gutkind JS: G-protein-coupled receptors and signaling networks: emerging paradigms, Trends Pharmacol Sci 22(7): 368–76, 2001 Marinissen MJ, Servitja JM, Offermanns S, Simon MI, Gutkind JS: Thrombin protease-activated receptor-1 signals through Gq and G13-initiated MAPK cascades regulating c-Jun expression to induce cell transformation, J Biol Chem 278 (47): 46814– 25, 2003 Martin CB, Mahon GM, Klinger MB, Kay RJ, Symons M, Der CJ et al: The thrombin receptor, PAR-1, causes transformation by activation of Rho-mediated signaling pathways, Oncogene 20 (16): 1953–63, 2001 Marius Grzelinski, Olaf Pinkenburg, Thomas Buch, et al: Critical Role of Ga12 and Ga13 for Human Small Cell Lung Cancer Cell Proliferation In vitro and Tumor Growth In vivo, Clin Cancer Res 16:1402-15, 2010 Maruyama Y, Nishida M, Sugimoto Y, Tanabe S, Turner JH, Kozasa T et al: Galpha(12/13) mediates alpha(1)-adrenergic receptorinduced cardiac hypertrophy, Circ Res 91 (10): 961–9, 2002 Meigs TE, Fields TA, McKee DD, Casey PJ: Interaction of Galpha12 and Galpha 13 with the cytoplasmic domain of cadherin provides a mechanism for beta -catenin release, Proc Natl Acad Sci USA 98 (2): 519–24, 2001 Meigs TE, Fedor-Chaiken M, Kaplan DD, Brackenbury R, Casey PJ: Galpha12 and Galpha13 negatively regulate the adhesive functions of cadherin, J Biol Chem 277 (27): 24594–600, 2002 Meigs TE, Juneja J, DeMarco CT, Stemmle LN, Kaplan DD, Casey PJ : Selective uncoupling of G alpha 12 from Rho-mediated signaling, J Biol Chem 280 (18): 18049–55, 2005 Michael P. Strathmann and Melvin I Simon: Gal2 and Ga13 subunits define a fourth class of G protein a subunits, Proc. Nall. Acad. Sci. USA 88: 5582-6, 1991 Milligan G, Mullaney I, Mitchell FM: Immunological identification of the alpha subunit of G13, a novel guanine nucleotide binding protein, FEBS Lett 297 (1–2): 186–188, 1992 Mills GB, Moolenaar WH : The emerging role of lysophosphatidic acid in cancer. Nat Rev Cancer 3 (8): 582–591, 2003 MJ Marinissen and J. Silvio Gutkind: G-protein-coupled receptors and signaling networks: emerging paradigms, TRENDS in Pharmacological Sciences, 22:368-76, 2002 Moers A, Nurnberg A, Goebbels S, Wettschureck N, Offermanns S: Galpha12/Galpha13 deficiency causes localized overmigration of neurons in the developing cerebral and cerebellar cortices, Mol Cell Biol 28 (5): 1480–1488, 2008 Nagao M, Kaziro Y, Itoh H: The Src family tyrosine kinase is involved in Rho-dependent activation of c-Jun N-terminal kinase by Galpha12, Oncogene 18 (31): 4425–4434, 1999 Neville et al: Oral and Maxillofacial pathology, 2nd edi, 315-76, 2002 Nikolaidou KK, Barrett K: A Rho GTPase signaling pathway is used reiteratively in epithelial folding and potentially selects the outcome of Rho activation, Curr Biol 14 (20): 1822–1826, 2004 Niv A, Sion-Vardi N, Gatot A et al: Identification and typing of human papillomavirus (HPV) in squamous cell carcinoma of the oral cavity and oropharynx, J Laryngol Otol 114: 41-6, 2000 Parks S, Wieschaus E: The Drosophila gastrulation gene concertina encodes a G alpha-like protein, Cell 64 (2): 447–458, 1991 Parkin DM, Bray F, Ferlay J, Pisani P.: Global cancer statistics, 2002, CA Cancer J Clin 55:74-108, 2002 Partridge M, Kiguwa S, Emilion G et al: New insights in p53 protein stabilization in oral squamous cell carcinoma, Oral Oncol 35:45-55, 1999 Pierce KL, Premont RT, Lefkowitz RJ : Seven-transmembrane receptors, Nat Rev Mol Cell Biol 3 (9): 639–50, 2002 Sankaranarayanan R, Mathew B, Varghese C, et al: Chemoprevention of oral leukoplakia with vitamin A and beta carotene: an assessment, Oral Oncol 33:231-6, 1977 Stemmle LN, Fields TA, Casey PJ (2006). The regulator of G protein signaling domain of axin selectively interacts with Galpha12 but not Galpha13, Mol Pharmacol 70 (4): 1461–8, 2006 Suwa H, Ohshio G, Imamura T, Watanabe G, Arii S, Imamura M et al: Overexpression of the rhoC gene correlates with progression of ductal adenocarcinoma of the pancreas, Br J Cancer 77 (1):147–152, 1998 Takashima S, Sugimoto N, Takuwa N, Okamoto Y, Yoshioka K, Takamura M et al: G12/13 and Gq mediate S1P2-induced inhibition of Rac and migration in vascular smooth muscle in a manner dependent on Rho but not Rho kinase. Cardiovasc Res 79 (4): 689–97, 2008 Radeff-Huang et al: G Protein Mediated Signaling Pathways in Lysophospholipid Induced Cell Proliferation and Survival, J. Cell. Biochem. 92: 949–66, 2004. Radhika V, Hee Ha J, Jayaraman M, Tsim ST, Dhanasekaran N:Mitogenic signaling by lysophosphatidic acid (LPA) involves Gα12, Oncogene 24:4597–603, 2005 Schoelch ML, Sekandari N, Regezi JA, et al: Laser management of oral leukoplakias: a follow-up study of 70 patients, Laryngoscope 109:949-53, 1999 Spicher K, Kalkbrenner F, Zobel A, Harhammer R, Nurnberg B, Soling A et al: G12 and G13 alpha-subunits are immunochemically detectable in most membranes of various mammalian cells and tissues. Biochem Biophys Res Commun 198 (3): 906–14, 1994 Shah JP et al: Head and neck surgery 2nd edi, 143-234, 355-93, 2002 Shiu MN, Chen TH, Chang SH, et al: Risk factors for leukoplakia and malignant transformation to oral carcinoma: a leukoplakia cohort in Taiwan, Br J Cancer 82:1871-4, 2000 Susana R. Neves, et al.: G Protein Pathways, Science 296, 1636-9,2002 Thiery JP: Epithelial-mesenchymal transitions in tumour progression, Nat Rev Cancer 2 (6): 442–454, 2002 Tralongo V, Rodolico V, Luciani A, et al: Prognostic factors in oral squamous cell carcinoma. A review of the literature, Anticancer Res 19:3503-10, 1999 K.A.A.S Warnakulasuriya et al: oral cancer: An alarming lack of public awareness towards oral cancer, Br Dent J 187(6):319-22,1999 Xu J, Wang F, Van Keymeulen A, Herzmark P, Straight A, Kelly K et al: Divergent signals and cytoskeletal assemblies regulate selforganizing polarity in neutrophils, Cell 114 (2): 201–14, 2003 Xu N, Bradley L, Ambdukar I, Gutkind JS (1993). A mutant alpha subunit of G12 potentiates the eicosanoid pathway and is highly oncogenic in NIH 3T3 cells, Proc Natl Acad Sci USA 90 (14): 6741–5, 1993 Yanamadala V, Negoro H, Gunaratnam L, Kong T, Denker BM: Galpha12 stimulates apoptosis in epithelial cells through JNK1-mediated Bcl-2 degradation and up-regulation of IkappaBalpha, J Biol Chem 282 (33): 24352–63, 2007 Yehia Daaka: G proteins in cancer: the prostate cancer paradigm, Sci. STKE 206: re2, 2004 Yu et al: Gene expression alterations in prostate cancer predicting tumor aggression and preceding development of malignancy, J Clin Oncol 22:2790-9, 2004 Zhu D, Kosik KS, Meigs TE, Yanamadala V, Denker BM: Galpha12 directly interacts with PP2A: evidence FOR Galpha12-stimulated PP2A phosphatase activity and dephosphorylation ofmicrotubule-associated protein, tau, J Biol Chem 279 (53): 54983–6, 2004 Zhu D, Tate RI, Ruediger R, Meigs TE, Denker BM: Domains necessary for Galpha12 binding and stimulation of protein phosphatase-2A (PP2A): Is Galpha12 a novel regulatory subunit of PP2A? Mol Pharmacol 71 (5): 1268–76, 2007 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46785 | - |
| dc.description.abstract | 背景
Gα12為G蛋白家族中屬於Gα12/13次家族的一類，在人體正常的生理機能如血壓調控、肌肉收縮及胚胎發育過程中細胞正常的遷移，在神經系統的發育扮演重要角色。Gα12已知可能促進腫瘤生長(proliferation)及轉移(metastasis)，在人體癌症如乳癌、攝護腺癌及鼻咽癌等的表現量都有提高的情形。本研究欲探討口腔癌中Gα12蛋白於病理組織中的表現量及與臨床各參數和存活時間的關係。 材料與方法 本研究利用免疫組織化學染色方法，探討Gα12於100例口腔鱗狀細胞癌(OSCC)、45例口腔上皮變異(OED)及36例正常口腔黏膜(NOM)中的表現，並以陽性染色指標Labeling index紀錄其染色程度。利用卡方檢定(Chi-square test)、Kaplan-Meier 存活率方法及Cox proportional hazard regression model來分析Gα12的表現與口腔鱗狀細胞癌患者臨床病理參數及存活率之相關性，並試圖尋找影響存活時間的獨立預後因子。 結果 在正常口腔黏膜Gα12的平均陽性標記指數為6.78% (最低)，口腔上皮變異組的平均陽性標記指數為21.78%，口腔癌組的平均陽性標記指數為 51.27% (最高)，可以看出從正常口腔黏膜、上皮變異至口腔癌，這個癌化的過程，Gα12的表現增加的情形，且達統計意義(P < 0.001)。Gα12的表現和患者的腫瘤大小與侵犯深度(T status)、淋巴結轉移與否(N status)、癌症臨床分期(Stage)有統計上相關。 各項臨床參數在Cox proportional hazard regression model分析中，以單變項分析後發現，腫瘤大小與侵犯深度、淋巴結轉移與否、腫瘤臨床分期及Gα12的陽性染色指標(LI)都與存活率有關；且以上四者經多變項分析均為影響存活時間的獨立因子(independent factor)(p<0.05)；Gα12陽性染色指標若>50%，影響病人存活的危險率(hazard ration)高達5.289倍(p=0.045)。同樣地以Cox proportional hazard regression model分析組織病理中五項風險因子，由單變項分析中發現，原發腫瘤侵犯血管淋巴系統(lymphovascular invasion)、具有兩顆以上之轉移淋巴結(metastatic lymph nodes≧2)兩項與存活率有關，再經多變項分析後，發現此二者也是影響病患存活率的獨立因子。 若以Kaplan-Meier plots來觀察臨床及組織病理各項參數、Gα12的陽性染色指標與病人總存活時間(overall survival)，經Log-Rank Test分析後，腫瘤大小與侵犯深度、淋巴結轉移與否、腫瘤臨床分期、原發腫瘤是否侵犯血管淋巴系統及是否具有兩顆以上之轉移淋巴結均影響病人的存活時間，且達統計上之意義(p<0.05)。而本研究探討的Gα12的陽性染色指標(LI)若>50%，則明顯降低了口腔癌病人的存活率(p=0.0009)。 結論 在本研究中，Gα12的陽性染色指標在口腔癌病理標本中的表現較高且具統計上意義，且與口腔細胞癌患者在臨床參數、組織病理的風險因子及存活時間有關。在口腔癌患者而言，Gα12的表現量指向較短的存活時間；而Gα12在切片組織中的表現程度，或許可以做為口腔癌的預後指標(prognostic marker)。 | zh_TW |
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| dc.description.tableofcontents | 中文摘要V
英文摘要VII 第一章 序論及文獻回顧 9 1.1.口腔鱗狀細胞癌概論 9 1.1.1口腔鱗狀細胞癌簡介 9 1.1.2口腔鱗狀細胞癌之流行病學 10 1.1.2.1口腔鱗狀細胞癌之全球流行病學 10 1.1.2.2.口腔鱗狀細胞癌之台灣地區流行病學及疾病特色11 1.1.3口腔鱗狀細胞癌之危險因子及基因變異 11 1.1.4口腔鱗狀細胞癌之臨床分期 16 1.1.5現行對口腔鱗狀細胞之治療及預後 16 1.1.6口腔癌前病變 16 1.2 Gα12蛋白概論 17 1.2.1.1 G蛋白家族(G protein family) 17 1.2.1.2 G蛋白於細胞內之訊息傳遞(signaling tranduction)機制 19 1.2.2 Gα12在胚胎發育過程之角色19 1.2.3 Gα12之生理作用與其他疾病的關係 21 1.3 Gα12與癌症之關係 22 1.3.1 Gα12於細胞株(cell lines)及動物實驗(animal models)之研究 23 1.3.2 Gα12於人類癌症之發現 25 第二章 材料與方法 28 2.1實驗樣本 28 2.2標本之固定與包埋 28 2.3免疫組織化學染色(immunohistochemistry stain) 28 2.3.1反應原理28 2.3.2初級抗體的選擇 29 2.3.3 抗原抗體結合之偵測系統 29 2.3.4實驗步驟 30 2.4免疫組織化學染色之觀察與記錄 32 2.4.1觀察 32 2.4.2染色程度之定量 32 2.4.3臨床變數之紀錄 32 2.4.4統計分析 33 第三章 結果 35 3.1 Gα12於口腔鱗狀細胞癌(OSCC)、口腔上皮變異(DOE)及正常口腔上皮(NOM)之表現 35 3.2 Gα12免疫組織化學染色結果與患者臨床及組織病理各項參數之關係36 3.3 Gα12免疫組織化學染色與臨床、組織病理參數之單變量、多變量分析 40 3.4Gα12表現與存活時間分析 41 第四章 討論 42 第五章 結論 42 第六章 附表與附圖 47 參考文獻 62 | |
| dc.language.iso | zh-TW | |
| dc.subject | 口腔上皮變異 | zh_TW |
| dc.subject | Gα12蛋白 | zh_TW |
| dc.subject | 口腔鱗狀細胞癌 | zh_TW |
| dc.subject | oral epithelial dysplasia | en |
| dc.subject | Gα12 | en |
| dc.subject | oral squamous cell carcinoma | en |
| dc.title | Gα12蛋白於口腔鱗狀細胞癌中之表現 | zh_TW |
| dc.title | Expression of Gα12 in oral squamous cell carcinoma | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張龍昌 | |
| dc.subject.keyword | Gα12蛋白,口腔鱗狀細胞癌,口腔上皮變異, | zh_TW |
| dc.subject.keyword | Gα12,oral squamous cell carcinoma,oral epithelial dysplasia, | en |
| dc.relation.page | 72 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-05 | |
| dc.contributor.author-college | 牙醫專業學院 | zh_TW |
| dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
| 顯示於系所單位： | 臨床牙醫學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-100-1.pdf 未授權公開取用 | 1.35 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。