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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 梁博煌(Po-Huang Liang) | |
| dc.contributor.author | Chu-Chin Wang | en |
| dc.contributor.author | 王楚鈞 | zh_TW |
| dc.date.accessioned | 2021-06-15T05:25:54Z | - |
| dc.date.available | 2010-07-20 | |
| dc.date.copyright | 2010-07-20 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-07-15 | |
| dc.identifier.citation | REFERENCES
(1) Hutterer, K. M. and Jorgenson, J. W. (2005). Separation of hyaluronic acid by ultrahigh-voltage capillary gel electrophoresis. Electrophoresis. 26, 2027-2033. (2) Meyer, K. and Palmer, J. W. (1934). The polysaccharide of the vitreous humor. J. Biol. Chem. 107, 629–634. (3) Toole, B. P. (1990). Hyaluronan and its binding proteins, the hyaladherins. Curr. Opin. Cell Biol. 2, 839–844. (4) Laurent, T. C. and Fraser, J. R. (1992). Hyaluronan. FASEB J. 6, 2397–2404. (5) Hall, C. L. and Turley, E. A. (1995) J. Neuro-Oncology 26, 221–229. (6) Lesley, J. and Kincade, H. R. (1993). CD44 and its interaction with extracellular matrix. Adv. Immunol. 54, 271–335. (7) Balazs, E. A. (1993). Viscosupplementation: a new concept in the treatment of osteoarthritis. J. Rheumatol. 39, 3–9. (8) Goa, K. L., and Benfield, P. (1994). Hyaluronic acid. A review of its pharmacology and use as a surgical aid in ophthalmology, and its therapeutic potential in joint disease and wound healing. Drugs 47, 536–566. (9) Volpi, N. and Maccari, F. (2003) Biochimie 85, 618–624. (10) Heldin, P., Laurent, T. C. and Heldin, C. (1989). Effect of growth factors on hyaluronan synthesis in cultured human fibroblasts. Biochem. J. 258, 919–922. (11) West, D. C., Hampson, I. N., Arnold, F. and Kumar, S. (1985). Angiogenesis induced by degradation products of hyaluronic acid. Science 228, 1324–1326. (12) Underhill, C. B. and Toole, B. P. (1982) J. Cell Phys. 110, 123–128. (13) Luo, Y. and Prestwich, G. D. (1999). Synthesis and selective cytotoxicity of a hyaluronic acid-antitumor bioconjugate. Bioconj. Chem. 10, 755–763. (14) Kakehi, K., Kinoshita, M. and Yasueda, S. I. (2003) J. Chrom. B., 797, 347–355. (15) DeAngelis, P. L. (1999). Hyaluronan synthases: fascinating glycosyltransferases from vertebrates, bacterial pathogens, and algal viruses. Cell Mol. Life Sci. 56, 670-682. (16) Heldermon, C., DeAngelis, P. L. and Weigel, P. H. (2001). Topological organization of the hyaluronan synthase from Streptococcus pyogenes. J. Biol. Chem. 276, 2037-2046. (17) Kshama, K. et al. (2006). Mutation of two intramembrane polar residues conserved within the hyaluronan synthase family alters hyaluronan product size. J. Biol. Chem. 281, 11755–11760. (18) Bitter, T. and Muir, H. M. (1962). A modified uronic acid carbazole reaction. Anal. Biochem. 4, 330–334. (19) Chong, B. F. and Nielsen, L. K. (2003). Amplifying the cellular reduction potential of Streptococcus zooepidemicus. J Biotechnol. 100,33-41. (20) Widner et al. (2005). Hyaluronic acid production in Bacillus subtilis. Appl Environ Microbiol. 71,3747-3752. (21) Lee, H. G. and Cowman, M. K. (1994). An agarose gel electrophoretic method for analysis of hyaluronan molecular weight distribution. Anal. Biochem. 219,278–287. (22) Stoolmiller and Dorfman. (1969). The biosynthesis of hyaluronic acid by Streptococcus. J Biol Chem. 244, 236-246. (23) Tlapak-Simmons et al. (1999). Purification and lipid dependence of the recombinant hyaluronan synthases from Streptococcus pyogenes and Streptococcus equisimilis. J Biol Chem. 274, 4239-4245. (24) Huimin, Yu and Gregory, Stephanopoulos. (2007). Metabolic engineering of Escherichia coli for biosynthesis of hyaluronic acid. Metab Eng. 10, 24-32. (25) DeAngelis, P. L. and Weigel, P. H. (1994). Immunochemical confirmation of the primary structure of streptococcal hyaluronan synthase and synthesis of high molecular weight product by the recombinant enzyme. Biochemistry 33, 9033–9039. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46728 | - |
| dc.description.abstract | 玻尿酸是一個重複β(1,3)-GlcNAc-β(1,4)- GlcA單元的線型多醣，可被發現於哺乳動物組織細胞外及細菌孢子內。玻尿酸由玻尿酸合成酶催化而成，其為具有二重功能把GlcNAc和GlcA二種醣的帶UDP先質接到延長中的玻尿酸長鏈。既然最近的醫藥級化妝品對於玻尿酸的需求甚殷，我們將Streptococcus pyogenes c203S之玻尿酸合成酶在大腸桿菌膜上表達來產生分泌的高分子量玻尿酸，並企圖使用隨意突變來增加其產量。 | zh_TW |
| dc.description.abstract | Hyaluronan (HA), a linear polysaccharide composed of β(1,3)-GlcNAc-β(1,4)- GlcA repeats, is found in the extracellular matrix of vertebrate tissues as well as the capsule of several pathogenic bacteria. HAs are synthesized by HA synthases (HASs), which are dual-action glycosyltransferases that catalyze the addition of two different sugars GlcA and GlcNAc from UDP-linked precursors to the growing HA chain. Since the recent medical and cosmetic uses of HA have largely increased its demand, as described in this invention, we have overexpressed spHAS from Gram positive Streptococcus pyogenes c203S on the membrane of E. coli to produce and secrete HA with high molecular weight into the media. Random mutagenesis by error-prone PCR was performed attempting to isolate the mutants with improved yield of HA. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T05:25:54Z (GMT). No. of bitstreams: 1 ntu-99-R97b46008-1.pdf: 1148333 bytes, checksum: 6e1b9fb40946bc5f627ea9593d685296 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | TABLE OF CONTENTS
中文摘要…………………………………………………………………………….1 ABSTRACT…….…………………………………………………………………….2 ABBREVIATION……………………………………………………………………3 INTRODUCTION…………………………………………………………………...4 MATERIAL AND METHODS……………………………………………………...8 RESULTS……………………………………………………………………………13 DISCUSSION……………………………………………………………………….19 REFERENCE……………………………………………………………………….22 FIGURE……………………………………………………………………………..25 POSTSCRIPT……………………………………………………………….......49 | |
| dc.language.iso | en | |
| dc.subject | 隨意突變 | zh_TW |
| dc.subject | 玻尿酸 | zh_TW |
| dc.subject | 大腸桿菌 | zh_TW |
| dc.subject | E. coli | en |
| dc.subject | Random mutagenesis | en |
| dc.subject | Hyaluronan | en |
| dc.title | 隨意突變表現於大腸桿菌之Streptococcus pyogenes玻尿酸合成酶企圖提高玻尿酸產量 | zh_TW |
| dc.title | Attempt of Increasing Yield of Hyaluronan by Randomly Mutating Streptococcus pyogenes Hyaluronan Synthase Expressed in E. coli | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 吳世雄,陳昭岑 | |
| dc.subject.keyword | 玻尿酸,大腸桿菌,隨意突變, | zh_TW |
| dc.subject.keyword | Hyaluronan,E. coli,Random mutagenesis, | en |
| dc.relation.page | 50 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-07-16 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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