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標題: | 設計胜肽模板誘導碳酸鈣之成晶 Peptide Template for Inducing the Nucleation of Calcium Carbonate |
作者: | Kan Wu 吳衎 |
指導教授: | 陳振中(Chun-Chung Chan) |
關鍵字: | 生物礦化,模板成晶,碳酸鈣,類澱粉狀蛋白纖維, biomineralization,nucleation,calcium carbonate,aragonite,peptide,amyloid fibril, |
出版年 : | 2015 |
學位: | 碩士 |
摘要: | 生物礦化的研究有許多重要的面向,像是生物礦物的多層次結構的鑑定,或是水溶液中之離子形成不同晶相或形貌之晶體的機制。在常溫常壓下生成的生物礦物的形貌比地質學上的礦物的形貌要複雜許多。所以我們希望發展一個仿生系統來研究無機礦物與有機軟組織之間的交互作用。在生物系統的啟發之下,我們設計了以胜肽組成的、β褶板結構的成晶模板,以此成晶模板來誘導碳酸鈣之成晶。目標胜肽的序列的正中間含有容易形成類澱粉狀蛋白纖維的片段,而在C端與N端則是有穀胺酸。我們將胜肽培養成類澱粉狀蛋白纖維,並使用氣體擴散沉澱法成功地在其上誘導碳酸鈣之成晶。透過穿透式電子顯微鏡,我們觀察到沿著纖維生長的碳酸鈣晶體。部分的晶體藉由選區電子散射被鑑定為霰石,而其他碳酸鈣常見的晶相皆未出現。碳酸鈣晶體與類澱粉狀蛋白纖維間的交互作用尚須固態核磁共振的量測才能知曉,也才能更進一步去了解其成晶的機制。 There are many important issues in biomineralization such as the characterization of the hierarchical structures of biominerals and the elucidation of the mechanisms of how ions from solution form the crystallites with specific crystalline phase and morphology. Biominerals formed in ambient environment have more delicate morphologies in comparison with their geological counterpart. It is highly desirable to develop a biomimetic system to study the interaction between the inorganic and organic species. As inspired by the nature, we have utilized a peptide-based beta-sheet template to induce calcium carbonate nucleation. The target peptide consists of a fibril forming sequence in the central region and glutamic acids at both C- and N-termini. We have successfully prepared the peptides in fibril state and the gas diffusion method is employed to induce the crystallization of calcium carbonate at the fibrils. Fibril-CaCO3 co-precipitation has been observed by TEM. The phase of the crystal has been confirmed to be aragonite by selected area electron diffraction. NMR measurements are underway to study the structural features at the organic-inorganic interface and to elucidate the mechanisms of the crystallization process. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/4657 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 化學系 |
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