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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊台鴻 | |
dc.contributor.author | Kai-Hsin Huang | en |
dc.contributor.author | 黃楷欣 | zh_TW |
dc.date.accessioned | 2021-06-15T05:12:29Z | - |
dc.date.available | 2015-07-28 | |
dc.date.copyright | 2010-07-28 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-07-22 | |
dc.identifier.citation | Alonso, L. and E. Fuchs (2006). 'The hair cycle.' J Cell Sci 119(Pt 3): 391-393.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46503 | - |
dc.description.abstract | 老化(aging)是一個哺乳類動物生長必經的過程,老化會降低器官及組織的功能。老化最主要的原因是由代謝所產生的氧化壓力所引起,它會降低組織及器官修復及再生的能力。
毛囊的發育相當的複雜,主要是由相當密切的上皮─間質交互作用來調控。真皮乳突細胞在毛囊再生扮演更重要的角色,真皮乳突細胞主要形成毛囊中的真皮乳突,而真皮乳突的功能為調控毛髮的生長及週期。雄性禿被認為是一種因為真皮乳突早熟老化的疾病,雄性禿導致真皮乳突細胞的早熟性老化。 將真皮乳突細胞暴露在不會致死的H2O2壓力下,會使真皮乳突細胞提早進入老化的狀態,並表現出一些細胞老化會表現的特徵,如細胞增生停止,細胞型態發生改變及表現高活性的senescence-associated β-galactosidase。H2O2誘導真皮乳突細胞老化後,會使p21蛋白質表現量上升,這使真皮乳突細胞進入細胞週期終止的狀態;並且提高p16蛋白質的表現,這主要是要維持真皮乳突細胞在細胞週期終止的細胞老化狀態。 在功能性上,老化的真皮乳突細胞在signature gene的mRNA表現上和未老化的真皮乳突細胞並沒有差異,但是老化的真皮乳突細胞在鹼性磷酸酶的活性及自我聚集能力上,皆比未老化的真皮乳突細胞還要差。這或許影響了真皮乳突細胞在誘導毛囊生成的能力。 這個誘導真皮乳突細胞老化的方式提供了我們在研究真皮乳突細胞的細胞老化對於其誘導毛囊生成的能力的關係上,提供了一種可行的方法。 | zh_TW |
dc.description.abstract | Aging is an inevitable process in mammals that impairs overall organ function. Aging mainly caused by oxidative stress and declines the ability of repair and regeneration.
The development of hair follicles is very complicated and depends on intimate epithelial- mesenchymal interaction. Dermal papilla cells (DPC) play an important role in hair follicle regeneration. DPCs form dermal papillae (DP) which regulate hair growth and hair cycle. Androgenetic alopecia (AGA) is considered a premature aging of dermal papillae. AGA results in premature senescence of dermal papilla cells. Premature senescence of dermal papilla cells is established by exposure to sublethal H2O2 stress. It is characterized by many biomarkers: cell proliferation arrest, cellular morphology, and senescence-associated β-galactosidase activity. H2O2-treated cells showing high level of p21 suggest cells enter a cell cycle arrest. Upregulation of p16 may be necessary for maintenance of the senescent cell cycle arrest. Functionally, senescent dermal papilla cells show no difference on DPC signature gene mRNA level, but decrease alkaline phosphatase activity and the ability of aggregation. These may have effect on the ability of hair follicle induction. This model provides a good way to investigate the relationship between cellular senescence of dermal papilla cells and the ability of hair follicle induction. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T05:12:29Z (GMT). No. of bitstreams: 1 ntu-99-R97548029-1.pdf: 1930130 bytes, checksum: ef0fdb99cbb88a052bac968b852a293a (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 目錄
誌謝 I 中文摘要 II Abstract III 第一章、序論 1 1.1.老化(aging) 1 1.1.1 老化理論 1 1.1.2 幹細胞老化(stem cell aging)及皮膚老化 2 1.2. 細胞老化(cellular senescence) 5 1.3. 雄性禿(androgenetic alopecia, AGA) 7 1.4. 上皮─間質交互作用(Epithelial-mesenchymal interaction) 9 1.4.1. 毛囊之發育 9 1.4.2. 毛囊之結構 11 1.4.3. 毛髮週期 13 1.4.4. 真皮乳突細胞之研究 14 第二章、實驗目的與設計 15 2.1. 實驗目的 15 2.2. 實驗假設 16 2.3. 實驗設計 16 第三章、材料與方法 18 3.1. 真皮乳突細胞的培養 18 3.2. 過氧化氫的處理 18 3.3. 細胞增生實驗 18 3.4. BrdU嵌入實驗 (BrdU Incorporation) 19 3.5. SA-β-Gal activity 19 3.6. 細胞週期分析 19 3.7. 西方墨點法 (Western blot) 19 3.8. 反轉錄聚合酶連鎖反應 (RT-PCR) 20 3.9. 鹼性磷酸酶之活性 (alkaline phosphatase activity) 22 3.10. 懸滴培養(hanging-drop)測試再聚集能力 22 3.11. 統計方法 22 第四章、實驗結果 23 4.1. 細胞增生 23 4.2. 細胞型態 25 4.3. BrdU嵌入實驗 27 4.4. SA-β-Gal activity 29 4.5. 細胞週期分析 31 4.6. 西方墨點法分析 33 4.7. 反轉錄聚合酶連鎖反應 (RT-PCR) 35 4.8. 鹼性磷酸酶之活性 37 4.9. 懸滴培養測試再聚集能力 40 第五章、討論 42 第六章、結論 45 第七章、參考文獻 46 圖目錄 圖1.1.2.1. 幹細胞老化模型 3 圖1.1.2.2. 幹細胞的功能性老化 4 圖1.4.1 毛囊的發育過程 10 圖1.4.2.毛囊的構造 12 圖2.2 實驗流程 17 圖4.1. 真皮乳突細胞(P4)之細胞增生 24 圖4.2. 細胞型態 26 圖4.3. BrdU incorporation 28 圖4.4 SA-β-Gal之活性 30 圖4.5. 細胞週期分析 32 圖4.6. 蛋白質表現 34 圖4.7. 未老化及H2O2誘導老化之真皮乳突細胞之mRNA表現 36 圖4.8.1. 鹼性磷酸酶之活性定量 38 圖4.8.2. 老化與不老化真皮乳突細胞之鹼性磷酸酶活性 39 圖4.9. 懸滴培養 41 表目錄 表1. 引子設計及序列 21 | |
dc.language.iso | zh-TW | |
dc.title | 利用過氧化氫誘導真皮乳突細胞之早熟性老化及功能性分析 | zh_TW |
dc.title | Premature Senescence of Dermal Papilla Cells Induced by Hydrogen Peroxide and Functional Analysis | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 紀秀華,廖怡華,吳君泰 | |
dc.subject.keyword | 老化,氧化壓力,上皮─間質交互作用,真皮乳突細胞,早熟性細胞老化,p21,p16, | zh_TW |
dc.subject.keyword | aging,oxidative stress,epithelial- mesenchymal interaction,dermal papilla cell,premature senescence,p21,p16, | en |
dc.relation.page | 54 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-07-23 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
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